This is a Validated Antibody Database (VAD) review about human CARM1, based on 7 published articles (read how Labome selects the articles), using CARM1 antibody in all methods. It is aimed to help Labome visitors find the most suited CARM1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CARM1 synonym: PRMT4

Knockout validation
Cell Signaling Technology
mouse monoclonal (3H2)
  • western blot knockout validation; mouse; 1:2000; loading ...; fig 1c
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1b
  • immunoprecipitation; mouse; loading ...; fig 1c
Cell Signaling Technology CARM1 antibody (Cell Signaling, 12495) was used in western blot knockout validation on mouse samples at 1:2000 (fig 1c), in immunohistochemistry - frozen section on mouse samples (fig 1b) and in immunoprecipitation on mouse samples (fig 1c). G3 (Bethesda) (2022) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C31G9)
  • western blot; mouse; 1:1000; fig 7a
  • western blot knockout validation; human; 1:1000; fig 2a
  • chromatin immunoprecipitation; human; fig 3a
  • immunoprecipitation; human; fig 4a
Cell Signaling Technology CARM1 antibody (CST, 3379S) was used in western blot on mouse samples at 1:1000 (fig 7a), in western blot knockout validation on human samples at 1:1000 (fig 2a), in chromatin immunoprecipitation on human samples (fig 3a) and in immunoprecipitation on human samples (fig 4a). Nat Commun (2021) ncbi
Cell Signaling Technology
mouse monoclonal (3H2)
  • western blot knockout validation; human; 1:1000; loading ...; fig s1d
Cell Signaling Technology CARM1 antibody (Cell Signaling Technology, 12495) was used in western blot knockout validation on human samples at 1:1000 (fig s1d). Nat Commun (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C31G9)
  • western blot knockout validation; mouse; loading ...; fig s1d
  • western blot; human; loading ...; fig 1b
Cell Signaling Technology CARM1 antibody (Cell Signaling, 3379) was used in western blot knockout validation on mouse samples (fig s1d) and in western blot on human samples (fig 1b). Nature (2016) ncbi
Cell Signaling Technology
mouse monoclonal (3H2)
  • western blot knockout validation; mouse; 1:2000; loading ...; fig 1c
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1b
  • immunoprecipitation; mouse; loading ...; fig 1c
Cell Signaling Technology CARM1 antibody (Cell Signaling, 12495) was used in western blot knockout validation on mouse samples at 1:2000 (fig 1c), in immunohistochemistry - frozen section on mouse samples (fig 1b) and in immunoprecipitation on mouse samples (fig 1c). G3 (Bethesda) (2022) ncbi
domestic rabbit monoclonal (C31G9)
  • western blot; mouse; 1:1000; fig 7a
  • western blot knockout validation; human; 1:1000; fig 2a
  • chromatin immunoprecipitation; human; fig 3a
  • immunoprecipitation; human; fig 4a
Cell Signaling Technology CARM1 antibody (CST, 3379S) was used in western blot on mouse samples at 1:1000 (fig 7a), in western blot knockout validation on human samples at 1:1000 (fig 2a), in chromatin immunoprecipitation on human samples (fig 3a) and in immunoprecipitation on human samples (fig 4a). Nat Commun (2021) ncbi
mouse monoclonal (3H2)
  • proximity ligation assay; human; 1:1000; fig 4c
Cell Signaling Technology CARM1 antibody (CST, 12495) was used in proximity ligation assay on human samples at 1:1000 (fig 4c). Nat Commun (2021) ncbi
mouse monoclonal (3H2)
  • western blot knockout validation; human; 1:1000; loading ...; fig s1d
Cell Signaling Technology CARM1 antibody (Cell Signaling Technology, 12495) was used in western blot knockout validation on human samples at 1:1000 (fig s1d). Nat Commun (2021) ncbi
domestic rabbit monoclonal (C31G9)
  • western blot; human; loading ...; fig 5b, 5f
Cell Signaling Technology CARM1 antibody (Cell Signaling, C31G9) was used in western blot on human samples (fig 5b, 5f). elife (2019) ncbi
domestic rabbit monoclonal (C31G9)
  • immunohistochemistry - paraffin section; human; loading ...; fig 1a
  • western blot; human; loading ...; fig 2f
Cell Signaling Technology CARM1 antibody (Cell Signaling, 3379) was used in immunohistochemistry - paraffin section on human samples (fig 1a) and in western blot on human samples (fig 2f). Tumour Biol (2017) ncbi
mouse monoclonal (3H2)
  • chromatin immunoprecipitation; human; loading ...; fig 3d
  • immunoprecipitation; human; loading ...; fig 3c
Cell Signaling Technology CARM1 antibody (CST, 12495) was used in chromatin immunoprecipitation on human samples (fig 3d) and in immunoprecipitation on human samples (fig 3c). Oncogene (2017) ncbi
domestic rabbit monoclonal (C31G9)
  • western blot; human; loading ...; fig 3c
Cell Signaling Technology CARM1 antibody (Cell Signaling, 3379) was used in western blot on human samples (fig 3c). Oncogene (2017) ncbi
mouse monoclonal (3H2)
  • ChIP-Seq; mouse; loading ...; fig s6e
  • immunoprecipitation; mouse; loading ...; fig 4a
  • immunocytochemistry; mouse; loading ...; fig 4b
Cell Signaling Technology CARM1 antibody (Cell Signaling, 12495) was used in ChIP-Seq on mouse samples (fig s6e), in immunoprecipitation on mouse samples (fig 4a) and in immunocytochemistry on mouse samples (fig 4b). Nature (2016) ncbi
domestic rabbit monoclonal (C31G9)
  • western blot knockout validation; mouse; loading ...; fig s1d
  • western blot; human; loading ...; fig 1b
Cell Signaling Technology CARM1 antibody (Cell Signaling, 3379) was used in western blot knockout validation on mouse samples (fig s1d) and in western blot on human samples (fig 1b). Nature (2016) ncbi
Articles Reviewed
  1. Jamet S, Ha S, Ho T, Houghtaling S, Timms A, Yu K, et al. The Arginine Methyltransferase Carm1 is Necessary for Heart Development. G3 (Bethesda). 2022;: pubmed publisher
  2. Lin J, Liu H, Fukumoto T, Zundell J, Yan Q, Tang C, et al. Targeting the IRE1α/XBP1s pathway suppresses CARM1-expressing ovarian cancer. Nat Commun. 2021;12:5321 pubmed publisher
  3. Yin S, Liu L, Brobbey C, PALANISAMY V, Ball L, Olsen S, et al. PRMT5-mediated arginine methylation activates AKT kinase to govern tumorigenesis. Nat Commun. 2021;12:3444 pubmed publisher
  4. Cai X, Zhang T, Kim E, Jiang M, Wang K, Wang J, et al. A chemical probe of CARM1 alters epigenetic plasticity against breast cancer cell invasion. elife. 2019;8: pubmed publisher
  5. Zhang M, Wu W, Gao M, Zhang J, Ding X, Zhu R, et al. Coactivator-associated arginine methyltransferase 1 promotes cell growth and is targeted by microRNA-195-5p in human colorectal cancer. Tumour Biol. 2017;39:1010428317694305 pubmed publisher
  6. Hiken J, McDonald J, Decker K, Sanchez C, Hoog J, VanderKraats N, et al. Epigenetic activation of the prostaglandin receptor EP4 promotes resistance to endocrine therapy for breast cancer. Oncogene. 2017;36:2319-2327 pubmed publisher
  7. Shin H, Kim H, Oh S, Lee J, Kee M, Ko H, et al. AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy. Nature. 2016;534:553-7 pubmed publisher