This is a Validated Antibody Database (VAD) review about human CAPN1, based on 30 published articles (read how Labome selects the articles), using CAPN1 antibody in all methods. It is aimed to help Labome visitors find the most suited CAPN1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CAPN1 synonym: CANP; CANP1; CANPL1; SPG76; muCANP; muCL

Knockout validation
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 4e
Cell Signaling Technology CAPN1 antibody (Cell Signaling, 2556) was used in western blot knockout validation on mouse samples at 1:1000 (fig 4e). Cell Rep (2016) ncbi
Invitrogen
mouse monoclonal (15C10)
  • western blot; human; 1:1000; loading ...; fig 3a
In order to determine the role of calcium regulation in sporadic inclusion body myositis, Invitrogen CAPN1 antibody (Thermo Fischer, MA1-12434) was used in western blot on human samples at 1:1000 (fig 3a). Acta Neuropathol Commun (2017) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; domestic sheep; 1:1000; loading ...; fig 3
In order to report the effects of phosphorylation on the activity of calpain and its sensitivity to temperature and calcium ions, Invitrogen CAPN1 antibody (Thermo Scientific, MA3-940) was used in western blot on domestic sheep samples at 1:1000 (fig 3). Food Chem (2017) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; domestic sheep; 1:5000
In order to study the effects of tannins on meat quality, Invitrogen CAPN1 antibody (Fisher Scientific, MA3-940) was used in western blot on domestic sheep samples at 1:5000. J Sci Food Agric (2016) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; pigs
Invitrogen CAPN1 antibody (Thermo Scientific, 9A4H8D3) was used in western blot on pigs samples . Int J Legal Med (2016) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; pigs ; 1:10000
In order to investigate the differences in calpain system, desmin degradation, pH values and water holding capacity between muscles of commercial Meishan and Duroc x Landrace x Yorkshire crossbred pigs, Invitrogen CAPN1 antibody (Affinity Bioreagents, MA3-940) was used in western blot on pigs samples at 1:10000. Anim Sci J (2016) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; bovine; 1:5000
In order to determine the effect of calcium lactate/phosphate enhancement on the quality of beef round cuts in high-oxygen modified atmosphere, Invitrogen CAPN1 antibody (Thermo, MA3-940) was used in western blot on bovine samples at 1:5000. Meat Sci (2015) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; pigs ; 1:10000
In order to investigate the differences in protein modifications between pale, soft, and exudative and red, firm, and nonexudative pork during postmortem aging, Invitrogen CAPN1 antibody (Affinity Bioreagents, MA3-940) was used in western blot on pigs samples at 1:10000. J Anim Sci (2014) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; pigs ; 1:10000
In order to investigate the effect of nitric oxide (NO) on calpain activation, protein proteolysis, and oxidation in porcine muscles, Invitrogen CAPN1 antibody (Aynity Bioreagents, MA3-940) was used in western blot on pigs samples at 1:10000. J Agric Food Chem (2014) ncbi
mouse monoclonal (9A4H8D3)
  • immunocytochemistry; rat
  • western blot; rat
In order to study the distinct roles of different calpain isoforms in synaptic neuroprotection and extrasynaptic neurodegeneration mediated by the NMDA receptor, Invitrogen CAPN1 antibody (Thermo Scientific, MA3-940) was used in immunocytochemistry on rat samples and in western blot on rat samples . J Neurosci (2013) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5a
Abcam CAPN1 antibody (Abcam, ab39170) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 5a). J Comp Neurol (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1a
In order to suggest that calpain promotes the dynamic flux of Atg9/Bif-1 vesicles from the Golgi toward the budding autophagosome, Abcam CAPN1 antibody (Abcam, ab28257) was used in western blot on human samples (fig 1a). Biol Open (2017) ncbi
domestic rabbit polyclonal
  • western blot; pigs ; loading ...; fig 1e
Abcam CAPN1 antibody (abcam, ab28257) was used in western blot on pigs samples (fig 1e). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 3
Abcam CAPN1 antibody (Abcam, 28257) was used in western blot on human samples (fig 3). Acta Neuropathol Commun (2016) ncbi
domestic rabbit monoclonal (EPR3319)
  • immunohistochemistry - paraffin section; rat
In order to identify the mechanism of colistin-induced nephropathy, Abcam CAPN1 antibody (Abcam, ab108400) was used in immunohistochemistry - paraffin section on rat samples . Antimicrob Agents Chemother (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (28F3)
  • western blot; human; fig 13c
Santa Cruz Biotechnology CAPN1 antibody (Santa Cruz, 28F3) was used in western blot on human samples (fig 13c). J Pineal Res (2018) ncbi
mouse monoclonal (P-6)
  • western blot; human; loading ...; fig 2d
In order to use SILAC immunoprecipitation to identify antibody targets expressed by vaccinated cancer patients, Santa Cruz Biotechnology CAPN1 antibody (Santa Cruz, sc-81171) was used in western blot on human samples (fig 2d). Cancer Immunol Res (2016) ncbi
mouse monoclonal (D-11)
  • western blot; mouse; 1:2000; fig 3
Santa Cruz Biotechnology CAPN1 antibody (Santa Cruz, sc-271313) was used in western blot on mouse samples at 1:2000 (fig 3). ASN Neuro (2013) ncbi
Enzo Life Sciences
mouse monoclonal (9A4H8D3)
  • western blot; bovine; 1:1000; loading ...; tbl 1
In order to examine the relationships among a list of 23 protein biomarkers with CIE-L*a*b* meat color traits and ultimate pH on muscles of Maine-Anjou cows, Enzo Life Sciences CAPN1 antibody (Alexis, 9A4H8D3) was used in western blot on bovine samples at 1:1000 (tbl 1). J Agric Food Chem (2017) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; mouse; 1:1000; fig 4
Enzo Life Sciences CAPN1 antibody (Enzo, ALX-804-050-R100) was used in western blot on mouse samples at 1:1000 (fig 4). Nat Commun (2015) ncbi
mouse monoclonal (9A4H8D3)
  • western blot; bovine; 1:1000
Enzo Life Sciences CAPN1 antibody (Alexis, 9A4H8D3) was used in western blot on bovine samples at 1:1000. J Agric Food Chem (2014) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:2000; fig 5a
Cell Signaling Technology CAPN1 antibody (Cell Signaling, 2556) was used in western blot on human samples at 1:2000 (fig 5a). Nat Commun (2021) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:500; loading ...; fig 2
Cell Signaling Technology CAPN1 antibody (Ozyme, 2556S) was used in western blot on rat samples at 1:500 (fig 2). elife (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 3a
Cell Signaling Technology CAPN1 antibody (Cell Signaling, 2556) was used in western blot on mouse samples (fig 3a). Nature (2019) ncbi
domestic rabbit polyclonal
Cell Signaling Technology CAPN1 antibody (Cell Signaling, 2556S) was used . J Appl Physiol (1985) (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig 5
Cell Signaling Technology CAPN1 antibody (CST, 2556) was used in western blot on human samples at 1:1000 (fig 5). Cell Death Dis (2016) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 4e
Cell Signaling Technology CAPN1 antibody (Cell Signaling, 2556) was used in western blot knockout validation on mouse samples at 1:1000 (fig 4e). Cell Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:500; fig 3
Cell Signaling Technology CAPN1 antibody (Cell Signaling, 2556) was used in western blot on rat samples at 1:500 (fig 3). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:1000; fig 4
Cell Signaling Technology CAPN1 antibody (Cell signaling, 2556S) was used in western blot on rat samples at 1:1000 (fig 4). Cell Death Dis (2016) ncbi
Leica Biosystems
  • western blot; human; loading ...
Leica Biosystems CAPN1 antibody (Novocastra, NCL-CALP-12A2) was used in western blot on human samples . Muscle Nerve (2017) ncbi
  • western blot; human; loading ...
Leica Biosystems CAPN1 antibody (Novocastra, NCL-CALP-2C4) was used in western blot on human samples . Muscle Nerve (2017) ncbi
  • proximity ligation assay; human; 1:50; fig 6
In order to investigate the deficiency that affects SERCA expression and function in the skeletal muscle by Calpain 3, Leica Biosystems CAPN1 antibody (Leica Biosystems, NCL-CALP-12A2) was used in proximity ligation assay on human samples at 1:50 (fig 6). Expert Rev Mol Med (2016) ncbi
Articles Reviewed
  1. Hu D, Sun X, Magpusao A, Fedorov Y, Thompson M, Wang B, et al. Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease. Nat Commun. 2021;12:5305 pubmed publisher
  2. Plantier V, Sanchez Brualla I, Dingu N, Brocard C, Liabeuf S, Gackiere F, et al. Calpain fosters the hyperexcitability of motoneurons after spinal cord injury and leads to spasticity. elife. 2019;8: pubmed publisher
  3. Power M, Rogerson L, Schubert T, Berens P, Euler T, Paquet Durand F. Systematic spatiotemporal mapping reveals divergent cell death pathways in three mouse models of hereditary retinal degeneration. J Comp Neurol. 2019;: pubmed publisher
  4. Faraco G, Hochrainer K, Segarra S, Schaeffer S, Santisteban M, Menon A, et al. Dietary salt promotes cognitive impairment through tau phosphorylation. Nature. 2019;: pubmed publisher
  5. Ma X, Chang H, Wang Z, Xu S, Peng X, Zhang J, et al. Differential activation of the calpain system involved in individualized adaptation of different fast-twitch muscles in hibernating Daurian ground squirrels. J Appl Physiol (1985). 2019;127:328-341 pubmed publisher
  6. Proietti S, Catizone A, Masiello M, Dinicola S, Fabrizi G, Minini M, et al. Increase in motility and invasiveness of MCF7 cancer cells induced by nicotine is abolished by melatonin through inhibition of ERK phosphorylation. J Pineal Res. 2018;64:e12467 pubmed publisher
  7. Gagaoua M, Couvreur S, Le Bec G, Aminot G, Picard B. Associations among Protein Biomarkers and pH and Color Traits in Longissimus thoracis and Rectus abdominis Muscles in Protected Designation of Origin Maine-Anjou Cull Cows. J Agric Food Chem. 2017;65:3569-3580 pubmed publisher
  8. Amici D, Pinal Fernández I, Mázala D, Lloyd T, Corse A, Christopher Stine L, et al. Calcium dysregulation, functional calpainopathy, and endoplasmic reticulum stress in sporadic inclusion body myositis. Acta Neuropathol Commun. 2017;5:24 pubmed publisher
  9. Du M, Li X, Li Z, Li M, Gao L, Zhang D. Phosphorylation inhibits the activity of ?-calpain at different incubation temperatures and Ca2+ concentrations in vitro. Food Chem. 2017;228:649-655 pubmed publisher
  10. Marcassa E, Raimondi M, Anwar T, Eskelinen E, Myers M, Triolo G, et al. Calpain mobilizes Atg9/Bif-1 vesicles from Golgi stacks upon autophagy induction by thapsigargin. Biol Open. 2017;6:551-562 pubmed publisher
  11. Papadopoulos C, Laforet P, Nectoux J, Stojkovic T, Wahbi K, Carlier R, et al. Hyperckemia and myalgia are common presentations of anoctamin-5-related myopathy in French patients. Muscle Nerve. 2017;56:1096-1100 pubmed publisher
  12. Wan X, Wang D, Xiong Q, Xiang H, Li H, Wang H, et al. Elucidating a molecular mechanism that the deterioration of porcine meat quality responds to increased cortisol based on transcriptome sequencing. Sci Rep. 2016;6:36589 pubmed publisher
  13. Anta B, Pérez Rodríguez A, Castro J, García Domínguez C, Ibiza S, Martínez N, et al. PGA1-induced apoptosis involves specific activation of H-Ras and N-Ras in cellular endomembranes. Cell Death Dis. 2016;7:e2311 pubmed publisher
  14. Wang Y, Hersheson J, López D, Hammer M, Liu Y, Lee K, et al. Defects in the CAPN1 Gene Result in Alterations in Cerebellar Development and Cerebellar Ataxia in Mice and Humans. Cell Rep. 2016;16:79-91 pubmed publisher
  15. Toral Ojeda I, Aldanondo G, Lasa Elgarresta J, Lasa Fernández H, Fernandez Torron R, Lopez de Munain A, et al. Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle. Expert Rev Mol Med. 2016;18:e7 pubmed publisher
  16. Kurbatskaya K, Phillips E, Croft C, Dentoni G, Hughes M, Wade M, et al. Upregulation of calpain activity precedes tau phosphorylation and loss of synaptic proteins in Alzheimer's disease brain. Acta Neuropathol Commun. 2016;4:34 pubmed publisher
  17. Talbert E, Smuder A, Kwon O, Sollanek K, Wiggs M, Powers S. Blockage of the Ryanodine Receptor via Azumolene Does Not Prevent Mechanical Ventilation-Induced Diaphragm Atrophy. PLoS ONE. 2016;11:e0148161 pubmed publisher
  18. Shinde V, Kotla P, Strang C, Gorbatyuk M. Unfolded protein response-induced dysregulation of calcium homeostasis promotes retinal degeneration in rat models of autosomal dominant retinitis pigmentosa. Cell Death Dis. 2016;7:e2085 pubmed publisher
  19. Jhaveri D, Kim M, Thompson E, Huang L, Sharma R, Klein A, et al. Using Quantitative Seroproteomics to Identify Antibody Biomarkers in Pancreatic Cancer. Cancer Immunol Res. 2016;4:225-33 pubmed publisher
  20. Girard M, Dohme Meier F, Silacci P, Ampuero Kragten S, Kreuzer M, Bee G. Forage legumes rich in condensed tannins may increase n-3 fatty acid levels and sensory quality of lamb meat. J Sci Food Agric. 2016;96:1923-33 pubmed publisher
  21. Foditsch E, Saenger A, Monticelli F. Skeletal muscle proteins: a new approach to delimitate the time since death. Int J Legal Med. 2016;130:433-40 pubmed publisher
  22. Wang J, Yan X, Liu R, Fu Q, Zhou G, Zhang W. Differences in calpain system, desmin degradation and water holding capacity between commercial Meishan and Duroc × Landrace × Yorkshire crossbred pork. Anim Sci J. 2016;87:109-16 pubmed publisher
  23. Sato S, Kawamata Y, Takahashi A, Imai Y, Hanyu A, Okuma A, et al. Ablation of the p16(INK4a) tumour suppressor reverses ageing phenotypes of klotho mice. Nat Commun. 2015;6:7035 pubmed publisher
  24. Cruzen S, Kim Y, Lonergan S, Grubbs J, Fritchen A, Huff Lonergan E. Effect of early postmortem enhancement of calcium lactate/phosphate on quality attributes of beef round muscles under different packaging systems. Meat Sci. 2015;101:63-72 pubmed publisher
  25. Picard B, Gagaoua M, Micol D, Cassar Malek I, Hocquette J, Terlouw C. Inverse relationships between biomarkers and beef tenderness according to contractile and metabolic properties of the muscle. J Agric Food Chem. 2014;62:9808-18 pubmed publisher
  26. Yin Y, Zhang W, Zhou G, Guo B. Comparison of protein degradation, protein oxidation, and ?-calpain activation between pale, soft, and exudative and red, firm, and nonexudative pork during postmortem aging. J Anim Sci. 2014;92:3745-52 pubmed publisher
  27. Li Y, Liu R, Zhang W, Fu Q, Liu N, Zhou G. Effect of nitric oxide on ?-calpain activation, protein proteolysis, and protein oxidation of pork during post-mortem aging. J Agric Food Chem. 2014;62:5972-7 pubmed publisher
  28. Wang Y, Briz V, Chishti A, Bi X, Baudry M. Distinct roles for ?-calpain and m-calpain in synaptic NMDAR-mediated neuroprotection and extrasynaptic NMDAR-mediated neurodegeneration. J Neurosci. 2013;33:18880-92 pubmed publisher
  29. Li H, Zhang N, Sun G, Ding S. Inhibition of the group I mGluRs reduces acute brain damage and improves long-term histological outcomes after photothrombosis-induced ischaemia. ASN Neuro. 2013;5:195-207 pubmed publisher
  30. Ozkan G, Ulusoy S, Orem A, Alkanat M, Mungan S, Yulug E, et al. How does colistin-induced nephropathy develop and can it be treated?. Antimicrob Agents Chemother. 2013;57:3463-9 pubmed publisher