This is a Validated Antibody Database (VAD) review about human C9orf72, based on 5 published articles (read how Labome selects the articles), using C9orf72 antibody in all methods. It is aimed to help Labome visitors find the most suited C9orf72 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
C9orf72 synonym: ALSFTD; DENNL72; FTDALS; FTDALS1; guanine nucleotide exchange C9orf72; protein C9orf72

Knockout validation
GeneTex
mouse monoclonal
  • western blot knockout validation; human; loading ...; fig 2
  • immunohistochemistry; mouse; loading ...; fig 5b
GeneTex C9orf72 antibody (GeneTex, GTX632041) was used in western blot knockout validation on human samples (fig 2) and in immunohistochemistry on mouse samples (fig 5b). elife (2019) ncbi
GeneTex
mouse monoclonal (GT1553)
  • immunocytochemistry knockout validation; human; loading ...; fig 4s1
  • western blot knockout validation; human; loading ...; fig 2
GeneTex C9orf72 antibody (GeneTex, GTX634482) was used in immunocytochemistry knockout validation on human samples (fig 4s1) and in western blot knockout validation on human samples (fig 2). elife (2019) ncbi
Proteintech Group
mouse monoclonal (3D2H6)
  • immunocytochemistry knockout validation; human; loading ...; fig 4s1
  • western blot knockout validation; human; loading ...; fig 2
Proteintech Group C9orf72 antibody (Proteintech, PT66140) was used in immunocytochemistry knockout validation on human samples (fig 4s1) and in western blot knockout validation on human samples (fig 2). elife (2019) ncbi
GeneTex
mouse monoclonal (GT1553)
  • immunocytochemistry knockout validation; human; loading ...; fig 4s1
  • western blot knockout validation; human; loading ...; fig 2
GeneTex C9orf72 antibody (GeneTex, GTX634482) was used in immunocytochemistry knockout validation on human samples (fig 4s1) and in western blot knockout validation on human samples (fig 2). elife (2019) ncbi
mouse monoclonal
  • western blot knockout validation; human; loading ...; fig 2
  • immunohistochemistry; mouse; loading ...; fig 5b
GeneTex C9orf72 antibody (GeneTex, GTX632041) was used in western blot knockout validation on human samples (fig 2) and in immunohistochemistry on mouse samples (fig 5b). elife (2019) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:50
GeneTex C9orf72 antibody (Genetex, GTX119776) was used in immunohistochemistry - paraffin section on human samples at 1:50. Acta Neuropathol (2011) ncbi
Proteintech Group
mouse monoclonal (3D2H6)
  • immunocytochemistry knockout validation; human; loading ...; fig 4s1
  • western blot knockout validation; human; loading ...; fig 2
Proteintech Group C9orf72 antibody (Proteintech, PT66140) was used in immunocytochemistry knockout validation on human samples (fig 4s1) and in western blot knockout validation on human samples (fig 2). elife (2019) ncbi
mouse monoclonal (3D2H6)
  • western blot; mouse; 1:3000; loading ...; fig 3a
In order to elucidate the mechanism by which intronic hexanucleotide expansions in C9ORF72 contribute to amyotrophic lateral sclerosis, Proteintech Group C9orf72 antibody (Proteintech, 66140-1-Ig) was used in western blot on mouse samples at 1:3000 (fig 3a). Nat Neurosci (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 2
Proteintech Group C9orf72 antibody (Proteintech, 22637-1-AP) was used in western blot on mouse samples (fig 2). EMBO J (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:500; loading ...; fig 1c
  • western blot; human; 1:1000; loading ...; fig 1a
  • immunocytochemistry; mouse; 1:500; loading ...; fig 1c
  • western blot; mouse; 1:1000; loading ...; fig 1a
Proteintech Group C9orf72 antibody (Proteintech, 22637-1-AP) was used in immunocytochemistry on human samples at 1:500 (fig 1c), in western blot on human samples at 1:1000 (fig 1a), in immunocytochemistry on mouse samples at 1:500 (fig 1c) and in western blot on mouse samples at 1:1000 (fig 1a). Mol Neurobiol (2017) ncbi
MilliporeSigma
rabbit polyclonal
  • immunocytochemistry; mouse; loading ...; fig 2d
  • western blot; mouse; 1:2000; loading ...; fig 3a
In order to elucidate the mechanism by which intronic hexanucleotide expansions in C9ORF72 contribute to amyotrophic lateral sclerosis, MilliporeSigma C9orf72 antibody (SigmaAldrich, HPA023873) was used in immunocytochemistry on mouse samples (fig 2d) and in western blot on mouse samples at 1:2000 (fig 3a). Nat Neurosci (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:50
MilliporeSigma C9orf72 antibody (Sigma, HPA023873) was used in immunohistochemistry - paraffin section on human samples at 1:50. Acta Neuropathol (2011) ncbi
Articles Reviewed
  1. Laflamme C, McKeever P, Kumar R, Schwartz J, Kolahdouzan M, Chen C, et al. Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. elife. 2019;8: pubmed publisher
  2. Sivadasan R, Hornburg D, Drepper C, Frank N, Jablonka S, Hansel A, et al. C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons. Nat Neurosci. 2016;19:1610-1618 pubmed publisher
  3. Sellier C, Campanari M, Julie Corbier C, Gaucherot A, Kolb Cheynel I, Oulad Abdelghani M, et al. Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death. EMBO J. 2016;35:1276-97 pubmed publisher
  4. Maharjan N, Künzli C, Buthey K, Saxena S. C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress. Mol Neurobiol. 2017;54:3062-3077 pubmed publisher
  5. Al Sarraj S, King A, Troakes C, Smith B, Maekawa S, Bodi I, et al. p62 positive, TDP-43 negative, neuronal cytoplasmic and intranuclear inclusions in the cerebellum and hippocampus define the pathology of C9orf72-linked FTLD and MND/ALS. Acta Neuropathol. 2011;122:691-702 pubmed publisher