This is a Validated Antibody Database (VAD) review about human C5, based on 14 published articles (read how Labome selects the articles), using C5 antibody in all methods. It is aimed to help Labome visitors find the most suited C5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
C5 synonym: C5D; C5a; C5b; CPAMD4; ECLZB

Santa Cruz Biotechnology
mouse monoclonal (aE11)
  • immunohistochemistry; human; 1:50; loading ...; fig 2g
Santa Cruz Biotechnology C5 antibody (Santa Cruz, SC-58935) was used in immunohistochemistry on human samples at 1:50 (fig 2g). J Immunol Res (2018) ncbi
mouse monoclonal (2952)
  • immunohistochemistry; human; 1:50; loading ...; fig 2f
Santa Cruz Biotechnology C5 antibody (Santa Cruz, SC-52634) was used in immunohistochemistry on human samples at 1:50 (fig 2f). J Immunol Res (2018) ncbi
Abcam
mouse monoclonal
  • blocking or activating experiments; human; loading ...; fig 2d
Abcam C5 antibody (Abcam, ab135187) was used in blocking or activating experiments on human samples (fig 2d). Oncogene (2018) ncbi
mouse monoclonal (2952)
  • blocking or activating experiments; human; loading ...
In order to investigate the contribution of NLRP3 inflammasome activity to the T helper cell 1 response, Abcam C5 antibody (Abcam, ab11878) was used in blocking or activating experiments on human samples . Science (2016) ncbi
R&D Systems
mouse monoclonal (295003)
  • blocking or activating experiments; human; fig s7d
R&D Systems C5 antibody (R&D Systems, MAB2037) was used in blocking or activating experiments on human samples (fig s7d). Cell (2018) ncbi
Enzo Life Sciences
mouse monoclonal (aE11)
  • immunohistochemistry - frozen section; African green monkey; loading ...; fig 2d
  • ELISA; African green monkey; loading ...; fig 2c
Enzo Life Sciences C5 antibody (Enzo Life Sciences, aE11) was used in immunohistochemistry - frozen section on African green monkey samples (fig 2d) and in ELISA on African green monkey samples (fig 2c). Proc Natl Acad Sci U S A (2017) ncbi
BioLegend
mouse monoclonal (G25/2)
  • ELISA; African green monkey; loading ...; fig 2b
BioLegend C5 antibody (BioLegend, G25/2) was used in ELISA on African green monkey samples (fig 2b). Proc Natl Acad Sci U S A (2017) ncbi
LifeSpan Biosciences
mouse monoclonal (2952)
  • immunohistochemistry - frozen section; primate
  • immunohistochemistry - frozen section; rhesus macaque
LifeSpan Biosciences C5 antibody (Lifespan Biosciences, LS-C22320) was used in immunohistochemistry - frozen section on primate samples and in immunohistochemistry - frozen section on rhesus macaque samples . Hum Reprod (2011) ncbi
Invitrogen
mouse monoclonal (2952)
  • ELISA; human; 0.1 ug/ml; fig 9
In order to study the essential alternative complement pathway components regulated by TNF and how they impair activation of protein c in human glomerular endothelial cells, Invitrogen C5 antibody (Thermo Fisher, MA1-40162) was used in ELISA on human samples at 0.1 ug/ml (fig 9). J Immunol (2016) ncbi
Dako
mouse monoclonal (aE11)
  • immunohistochemistry; human; 1:100; loading ...; fig 2b
In order to assess the diagnostic value of myxovirus resistance A expression for dermatomyositis, Dako C5 antibody (Dako, M 0777) was used in immunohistochemistry on human samples at 1:100 (fig 2b). Neurology (2017) ncbi
mouse monoclonal (aE11)
  • immunohistochemistry - frozen section; mouse; 1:200; tbl 3
  • immunohistochemistry - frozen section; human; 1:200; tbl 3
In order to analyze amyotrophic lateral sclerosis and complement activation at the motor-end plates, Dako C5 antibody (Dako, M0777) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (tbl 3) and in immunohistochemistry - frozen section on human samples at 1:200 (tbl 3). J Neuroinflammation (2016) ncbi
mouse monoclonal (aE11)
  • immunohistochemistry; human; fig 6
Dako C5 antibody (DAKO, M0777/aE11) was used in immunohistochemistry on human samples (fig 6). Mol Vis (2016) ncbi
mouse monoclonal (aE11)
  • immunohistochemistry - frozen section; human; fig 1
In order to characterize dermatomyositis with or without anti-melanoma differentiation-associated gene 5 antibodies that show distinct NOS2 expression but a common interferon signature, Dako C5 antibody (Dako, aE11) was used in immunohistochemistry - frozen section on human samples (fig 1). Am J Pathol (2016) ncbi
mouse monoclonal (aE11)
  • immunocytochemistry; swine; 1:50; fig 3
Dako C5 antibody (Dako, M0777) was used in immunocytochemistry on swine samples at 1:50 (fig 3). J Immunol (2015) ncbi
mouse monoclonal (aE11)
  • flow cytometry; human; fig 3
Dako C5 antibody (DAKO, aE11) was used in flow cytometry on human samples (fig 3). Biochem Biophys Res Commun (2015) ncbi
Quidel
mouse monoclonal
  • neutralization; human
Quidel C5 antibody (Quidel, #A217) was used in neutralization on human samples . Am J Transplant (2015) ncbi
Articles Reviewed
  1. Melgaço J, Veloso C, Pacheco Moreira L, Vitral C, Pinto M. Complement System as a Target for Therapies to Control Liver Regeneration/Damage in Acute Liver Failure Induced by Viral Hepatitis. J Immunol Res. 2018;2018:3917032 pubmed publisher
  2. Zhao C, Li Y, Qiu W, He F, Zhang W, Zhao D, et al. C5a induces A549 cell proliferation of non-small cell lung cancer via GDF15 gene activation mediated by GCN5-dependent KLF5 acetylation. Oncogene. 2018;37:4821-4837 pubmed publisher
  3. Su S, Chen J, Yao H, Liu J, Yu S, Lao L, et al. CD10+GPR77+ Cancer-Associated Fibroblasts Promote Cancer Formation and Chemoresistance by Sustaining Cancer Stemness. Cell. 2018;172:841-856.e16 pubmed publisher
  4. Keshari R, Silasi R, Popescu N, Patel M, Chaaban H, Lupu C, et al. Inhibition of complement C5 protects against organ failure and reduces mortality in a baboon model of Escherichia coli sepsis. Proc Natl Acad Sci U S A. 2017;: pubmed publisher
  5. Uruha A, Nishikawa A, Tsuburaya R, Hamanaka K, Kuwana M, Watanabe Y, et al. Sarcoplasmic MxA expression: A valuable marker of dermatomyositis. Neurology. 2017;88:493-500 pubmed publisher
  6. Arbore G, West E, Spolski R, Robertson A, Klos A, Rheinheimer C, et al. T helper 1 immunity requires complement-driven NLRP3 inflammasome activity in CD4⁺ T cells. Science. 2016;352:aad1210 pubmed publisher
  7. Bahia El Idrissi N, Bosch S, Ramaglia V, Aronica E, Baas F, Troost D. Complement activation at the motor end-plates in amyotrophic lateral sclerosis. J Neuroinflammation. 2016;13:72 pubmed publisher
  8. Kochounian H, Zhang Z, Spee C, Hinton D, Fong H. Targeting of exon VI-skipping human RGR-opsin to the plasma membrane of pigment epithelium and co-localization with terminal complement complex C5b-9. Mol Vis. 2016;22:213-23 pubmed
  9. Allenbach Y, Leroux G, Suárez Calvet X, Preusse C, Gallardo E, Hervier B, et al. Dermatomyositis With or Without Anti-Melanoma Differentiation-Associated Gene 5 Antibodies: Common Interferon Signature but Distinct NOS2 Expression. Am J Pathol. 2016;186:691-700 pubmed publisher
  10. Sartain S, Turner N, Moake J. TNF Regulates Essential Alternative Complement Pathway Components and Impairs Activation of Protein C in Human Glomerular Endothelial Cells. J Immunol. 2016;196:832-45 pubmed publisher
  11. Georgiannakis A, Burgoyne T, Lueck K, Futter C, Greenwood J, Moss S. Retinal Pigment Epithelial Cells Mitigate the Effects of Complement Attack by Endocytosis of C5b-9. J Immunol. 2015;195:3382-9 pubmed publisher
  12. Thomas K, Valenzuela N, Gjertson D, Mulder A, Fishbein M, Parry G, et al. An Anti-C1s Monoclonal, TNT003, Inhibits Complement Activation Induced by Antibodies Against HLA. Am J Transplant. 2015;15:2037-49 pubmed publisher
  13. Stratton D, Moore C, Antwi Baffour S, Lange S, Inal J. Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9. Biochem Biophys Res Commun. 2015;460:589-95 pubmed publisher
  14. Keator C, Mah K, Ohm L, Slayden O. Estrogen and progesterone regulate expression of the endothelins in the rhesus macaque endometrium. Hum Reprod. 2011;26:1715-28 pubmed publisher