This is a Validated Antibody Database (VAD) review about human C3, based on 36 published articles (read how Labome selects the articles), using C3 antibody in all methods. It is aimed to help Labome visitors find the most suited C3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
C3 synonym: AHUS5; ARMD9; ASP; C3a; C3b; CPAMD1; HEL-S-62p

Knockout validation
Abcam
domestic rabbit monoclonal (EPR19394)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1a
Abcam C3 antibody (Abcam, ab200999) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1a). Front Immunol (2022) ncbi
Abcam
domestic rabbit monoclonal (EPR19394)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 3a
Abcam C3 antibody (Abcam, ab200999) was used in immunohistochemistry - paraffin section on mouse samples (fig 3a). Front Pharmacol (2022) ncbi
domestic rabbit monoclonal (EPR19394)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1a
Abcam C3 antibody (Abcam, ab200999) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1a). Front Immunol (2022) ncbi
domestic rabbit monoclonal (EPR19394)
  • western blot; mouse; 1:1000; loading ...; fig 2d
Abcam C3 antibody (Abcam, ab200999) was used in western blot on mouse samples at 1:1000 (fig 2d). Redox Biol (2022) ncbi
domestic rabbit monoclonal
  • immunohistochemistry; mouse; 1:100; fig 1c
  • western blot; mouse; 1:1000; loading ...; fig 2c
Abcam C3 antibody (Abcam, ab181147) was used in immunohistochemistry on mouse samples at 1:100 (fig 1c) and in western blot on mouse samples at 1:1000 (fig 2c). J Neuroinflammation (2021) ncbi
domestic rabbit monoclonal (EPR19394)
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig s16a
Abcam C3 antibody (Abcam, ab200999) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig s16a). Theranostics (2021) ncbi
domestic rabbit monoclonal (EPR19394)
  • western blot; mouse; 1:1000; loading ...; fig 4b
Abcam C3 antibody (Abcam, ab200999) was used in western blot on mouse samples at 1:1000 (fig 4b). Acta Neuropathol Commun (2021) ncbi
domestic rabbit monoclonal (EPR19394)
  • immunohistochemistry - free floating section; rat; 1:200; loading ...; fig 4c
  • western blot; rat; 1:1000; loading ...; fig 4b
Abcam C3 antibody (Abcam, ab200999) was used in immunohistochemistry - free floating section on rat samples at 1:200 (fig 4c) and in western blot on rat samples at 1:1000 (fig 4b). Theranostics (2020) ncbi
mouse monoclonal (7C10)
  • flow cytometry; human; fig 7e
Abcam C3 antibody (Abcam, 7C10) was used in flow cytometry on human samples (fig 7e). Sci Rep (2017) ncbi
mouse monoclonal (755)
  • western blot; human; fig 2c
In order to describe an exosome-mediated mechanism of drug-induced acquired chemoresistance in pancreatic cancer cells, Abcam C3 antibody (Abcam, ab11871) was used in western blot on human samples (fig 2c). Br J Cancer (2017) ncbi
mouse monoclonal (7C10)
  • flow cytometry; human; loading ...; fig 3A
Abcam C3 antibody (Abcam, 7C10) was used in flow cytometry on human samples (fig 3A). Proc Natl Acad Sci U S A (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:100; fig 2
In order to compare activation of complement both on their own NETs and bacteria via non-alternative and alternative pathways by NETosing neutrophils, Abcam C3 antibody (Abcam, ab97462) was used in western blot on human samples at 1:100 (fig 2). Front Immunol (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:50; tbl 3
  • immunohistochemistry - frozen section; mouse; 1:50; tbl 3
In order to analyze amyotrophic lateral sclerosis and complement activation at the motor-end plates, Abcam C3 antibody (Abcam, ab4212) was used in immunohistochemistry - frozen section on human samples at 1:50 (tbl 3) and in immunohistochemistry - frozen section on mouse samples at 1:50 (tbl 3). J Neuroinflammation (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; fig 2a
Abcam C3 antibody (Abcam, ab204121) was used in immunohistochemistry - paraffin section on human samples (fig 2a). PLoS ONE (2016) ncbi
mouse monoclonal (755)
  • western blot; human
Abcam C3 antibody (Abcam, ab11871) was used in western blot on human samples . Biomaterials (2011) ncbi
Santa Cruz Biotechnology
mouse monoclonal (B-9)
  • western blot; mouse; 1:200; loading ...; fig 5b
Santa Cruz Biotechnology C3 antibody (Santa Cruz, sc-28294) was used in western blot on mouse samples at 1:200 (fig 5b). J Neuroinflammation (2021) ncbi
mouse monoclonal (B-9)
  • immunohistochemistry - paraffin section; human; 1:50; loading ...; fig 5b
  • western blot; human; loading ...; fig 1a
Santa Cruz Biotechnology C3 antibody (Santa Cruz, sc-28294) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 5b) and in western blot on human samples (fig 1a). Front Immunol (2020) ncbi
mouse monoclonal (B-9)
  • western blot; human; 1:1000; loading ...; fig 2b, 3b
Santa Cruz Biotechnology C3 antibody (Santa Cruz, SC-28294) was used in western blot on human samples at 1:1000 (fig 2b, 3b). BMC Cancer (2019) ncbi
mouse monoclonal (K13/16)
  • immunohistochemistry; human; 1:50; loading ...; fig 2e
Santa Cruz Biotechnology C3 antibody (Santa Cruz, SC-47688) was used in immunohistochemistry on human samples at 1:50 (fig 2e). J Immunol Res (2018) ncbi
mouse monoclonal (K13/16)
  • western blot; human; loading ...; fig 2a
Santa Cruz Biotechnology C3 antibody (Santa Cruz, sc-47688) was used in western blot on human samples (fig 2a). Infect Dis Poverty (2016) ncbi
mouse monoclonal (B-9)
  • western blot; mouse; 1:10; loading ...; fig 4a
Santa Cruz Biotechnology C3 antibody (Santa Cruz Biotechnology, Sc-28294) was used in western blot on mouse samples at 1:10 (fig 4a). J Neuroinflammation (2014) ncbi
mouse monoclonal (B-9)
  • western blot; human
  • western blot; mouse
Santa Cruz Biotechnology C3 antibody (Santa Cruz Biotechnology, sc-28294) was used in western blot on human samples and in western blot on mouse samples . Age (Dordr) (2014) ncbi
Novus Biologicals
rat monoclonal (11H9)
  • immunohistochemistry; mouse; loading ...; fig 3c
  • immunohistochemistry; human; loading ...; fig 2a
Novus Biologicals C3 antibody (Novus Biologicals, NB200-540AF594) was used in immunohistochemistry on mouse samples (fig 3c) and in immunohistochemistry on human samples (fig 2a). Arthritis Res Ther (2021) ncbi
rat monoclonal (11H9)
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1g
Novus Biologicals C3 antibody (Novus, NB200-540) was used in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 1g). elife (2020) ncbi
domestic rabbit monoclonal (JF10-30)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s4e
Novus Biologicals C3 antibody (Novus, JF10-30) was used in immunohistochemistry - paraffin section on mouse samples (fig s4e). Nature (2019) ncbi
Invitrogen
mouse monoclonal (10C7)
  • flow cytometry; mouse; loading ...
In order to measure cell size and cell surface markers of human MSCs after intravenous injection in immune competent mice, Invitrogen C3 antibody (Thermo Scientific, MA1-70054) was used in flow cytometry on mouse samples . Cytotherapy (2017) ncbi
mouse monoclonal (6C9)
  • flow cytometry; human; loading ...; fig 2a
In order to study complement C1s and C1q binding in apoptotic cell phagocytosis, Invitrogen C3 antibody (Thermo Scientific, 6C9) was used in flow cytometry on human samples (fig 2a). Clin Immunol (2016) ncbi
mouse monoclonal (K13/16-5.7)
  • ELISA; human; 0.1 ug/ml; fig 9
In order to study the essential alternative complement pathway components regulated by TNF and how they impair activation of protein c in human glomerular endothelial cells, Invitrogen C3 antibody (Thermo Fisher, GAU 013-16-02) was used in ELISA on human samples at 0.1 ug/ml (fig 9). J Immunol (2016) ncbi
mouse monoclonal (6C9)
  • flow cytometry; human; 20 ug/ml
In order to study the efficacy of the serine protease C1s inhibitor TNT003 in the treatment of cold agglutinin disease, Invitrogen C3 antibody (Thermo Scientific, 6C9) was used in flow cytometry on human samples at 20 ug/ml. Blood (2014) ncbi
mouse monoclonal (D17/1)
  • ELISA; human
In order to investigate the effect of peanuts on anaphylactic shock and its mechanism, Invitrogen C3 antibody (Affinity BioReagents, GAU 017-01-02B) was used in ELISA on human samples . J Allergy Clin Immunol (2009) ncbi
mouse monoclonal (K13/16-5.7)
  • ELISA; mouse
  • ELISA; human
In order to investigate the effect of peanuts on anaphylactic shock and its mechanism, Invitrogen C3 antibody (Affinity BioReagents, GAU 013-16-02) was used in ELISA on mouse samples and in ELISA on human samples . J Allergy Clin Immunol (2009) ncbi
BioLegend
mouse monoclonal (3E7/C3b)
  • flow cytometry; hamsters; 1:50; loading ...; fig 2d
BioLegend C3 antibody (Biolegend, 846106) was used in flow cytometry on hamsters samples at 1:50 (fig 2d). Front Immunol (2021) ncbi
R&D Systems
domestic goat polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 5c
R&D Systems C3 antibody (R&D, AF2655) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 5c). Mol Neurobiol (2022) ncbi
domestic goat polyclonal
R&D Systems C3 antibody (R&D systems, AF2655) was used . Front Immunol (2021) ncbi
Dako
  • immunohistochemistry - frozen section; human; 1:10; loading ...; fig 2
Dako C3 antibody (DAKO, F0201) was used in immunohistochemistry - frozen section on human samples at 1:10 (fig 2). J Cell Mol Med (2019) ncbi
  • flow cytometry; human; loading ...; fig 7f
Dako C3 antibody (Dako, F0201) was used in flow cytometry on human samples (fig 7f). J Immunol (2018) ncbi
  • immunohistochemistry - frozen section; human
Dako C3 antibody (Dako Cytomation, F0201) was used in immunohistochemistry - frozen section on human samples . Transl Res (2014) ncbi
MP Biochemicals
polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 1b
MP Biochemicals C3 antibody (MP Biomedicals, 55500) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 1b). Cancer Res (2018) ncbi
Quidel
mouse monoclonal
  • flow cytometry; human; fig 5
Quidel C3 antibody (Quidel, A207) was used in flow cytometry on human samples (fig 5). PLoS ONE (2016) ncbi
Articles Reviewed
  1. Hong J, Zhang M, He Y, Jin Y, He Q, Zhang Y, et al. Qinghao-Biejia Herb Pair Alleviates Pristane-Induced Lupus-Like Disease and Associated Renal and Aortic Lesions in ApoE-/- Mice. Front Pharmacol. 2022;13:897669 pubmed publisher
  2. Torp M, Ranheim T, Schjalm C, Hjorth M, Heiestad C, Dalen K, et al. Intracellular Complement Component 3 Attenuated Ischemia-Reperfusion Injury in the Isolated Buffer-Perfused Mouse Heart and Is Associated With Improved Metabolic Homeostasis. Front Immunol. 2022;13:870811 pubmed publisher
  3. Huang S, Liu C, Wang Z, Fu Z, Britton W, Blomfield A, et al. REV-ERBα regulates age-related and oxidative stress-induced degeneration in retinal pigment epithelium via NRF2. Redox Biol. 2022;51:102261 pubmed publisher
  4. Pellegrini P, Hervera A, Varea O, Brewer M, L xf3 pez Soldado I, Guitart A, et al. Lack of p62 Impairs Glycogen Aggregation and Exacerbates Pathology in a Mouse Model of Myoclonic Epilepsy of Lafora. Mol Neurobiol. 2022;59:1214-1229 pubmed publisher
  5. Guo H, Fan Z, Wang S, Ma L, Wang J, Yu D, et al. Astrocytic A1/A2 paradigm participates in glycogen mobilization mediated neuroprotection on reperfusion injury after ischemic stroke. J Neuroinflammation. 2021;18:230 pubmed publisher
  6. Liu T, Yang M, Xia Y, Jiang C, Li C, Jiang Z, et al. Microarray-based analysis of renal complement components reveals a therapeutic target for lupus nephritis. Arthritis Res Ther. 2021;23:223 pubmed publisher
  7. Qin Z, Liu F, Blair R, Wang C, Yang H, Mudd J, et al. Endothelial cell infection and dysfunction, immune activation in severe COVID-19. Theranostics. 2021;11:8076-8091 pubmed publisher
  8. Takahashi K, Nakamura S, Otsu W, Shimazawa M, Hara H. Progranulin deficiency in Iba-1+ myeloid cells exacerbates choroidal neovascularization by perturbation of lysosomal function and abnormal inflammation. J Neuroinflammation. 2021;18:164 pubmed publisher
  9. Qiu W, Luo S, Ma S, Saminathan P, Li H, Gunnersen J, et al. The Sez6 Family Inhibits Complement by Facilitating Factor I Cleavage of C3b and Accelerating the Decay of C3 Convertases. Front Immunol. 2021;12:607641 pubmed publisher
  10. Park J, Kam T, Lee S, Park H, Oh Y, Kwon S, et al. Blocking microglial activation of reactive astrocytes is neuroprotective in models of Alzheimer's disease. Acta Neuropathol Commun. 2021;9:78 pubmed publisher
  11. Griffin P, Sheehan P, Dimitry J, Guo C, Kanan M, Lee J, et al. REV-ERBα mediates complement expression and diurnal regulation of microglial synaptic phagocytosis. elife. 2020;9: pubmed publisher
  12. Shahulhameed S, Vishwakarma S, Chhablani J, Tyagi M, Pappuru R, Jakati S, et al. A Systematic Investigation on Complement Pathway Activation in Diabetic Retinopathy. Front Immunol. 2020;11:154 pubmed publisher
  13. Zhang L, Pan J, Mamtilahun M, Zhu Y, Wang L, Venkatesh A, et al. Microglia exacerbate white matter injury via complement C3/C3aR pathway after hypoperfusion. Theranostics. 2020;10:74-90 pubmed publisher
  14. Aykut B, Pushalkar S, Chen R, Li Q, Abengozar R, Kim J, et al. The fungal mycobiome promotes pancreatic oncogenesis via activation of MBL. Nature. 2019;574:264-267 pubmed publisher
  15. van den Hoogen P, de Jager S, Huibers M, Schoneveld A, Puspitasari Y, Valstar G, et al. Increased circulating IgG levels, myocardial immune cells and IgG deposits support a role for an immune response in pre- and end-stage heart failure. J Cell Mol Med. 2019;23:7505-7516 pubmed publisher
  16. Zhao P, Wu J, Lu F, Peng X, Liu C, Zhou N, et al. The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer. BMC Cancer. 2019;19:201 pubmed publisher
  17. Melgaço J, Veloso C, Pacheco Moreira L, Vitral C, Pinto M. Complement System as a Target for Therapies to Control Liver Regeneration/Damage in Acute Liver Failure Induced by Viral Hepatitis. J Immunol Res. 2018;2018:3917032 pubmed publisher
  18. Spirig R, Campbell I, Koernig S, Chen C, Lewis B, BUTCHER R, et al. rIgG1 Fc Hexamer Inhibits Antibody-Mediated Autoimmune Disease via Effects on Complement and FcγRs. J Immunol. 2018;200:2542-2553 pubmed publisher
  19. Kwak J, Laskowski J, Li H, McSharry M, Sippel T, Bullock B, et al. Complement Activation via a C3a Receptor Pathway Alters CD4+ T Lymphocytes and Mediates Lung Cancer Progression. Cancer Res. 2018;78:143-156 pubmed publisher
  20. Shahini N, Michelsen A, Nilsson P, Ekholt K, Gullestad L, Broch K, et al. The alternative complement pathway is dysregulated in patients with chronic heart failure. Sci Rep. 2017;7:42532 pubmed publisher
  21. Patel G, Khan M, Bhardwaj A, Srivastava S, Zubair H, Patton M, et al. Exosomes confer chemoresistance to pancreatic cancer cells by promoting ROS detoxification and miR-155-mediated suppression of key gemcitabine-metabolising enzyme, DCK. Br J Cancer. 2017;116:609-619 pubmed publisher
  22. Harboe M, Johnson C, Nymo S, Ekholt K, Schjalm C, Lindstad J, et al. Properdin binding to complement activating surfaces depends on initial C3b deposition. Proc Natl Acad Sci U S A. 2017;114:E534-E539 pubmed publisher
  23. Leibacher J, Dauber K, Ehser S, Brixner V, Kollar K, Vogel A, et al. Human mesenchymal stromal cells undergo apoptosis and fragmentation after intravenous application in immune-competent mice. Cytotherapy. 2017;19:61-74 pubmed publisher
  24. Yuen J, Pluthero F, Douda D, Riedl M, Cherry A, Ulanova M, et al. NETosing Neutrophils Activate Complement Both on Their Own NETs and Bacteria via Alternative and Non-alternative Pathways. Front Immunol. 2016;7:137 pubmed publisher
  25. Chen C, Lv Y, Shi Q, Zhou W, Xiao K, Sun J, et al. Low activity of complement in the cerebrospinal fluid of the patients with various prion diseases. Infect Dis Poverty. 2016;5:35 pubmed publisher
  26. Bahia El Idrissi N, Bosch S, Ramaglia V, Aronica E, Baas F, Troost D. Complement activation at the motor end-plates in amyotrophic lateral sclerosis. J Neuroinflammation. 2016;13:72 pubmed publisher
  27. Li J, Ge X, Wang X, Liu X, Ma J. Complement System in the Pathogenesis of Benign Lymphoepithelial Lesions of the Lacrimal Gland. PLoS ONE. 2016;11:e0148290 pubmed publisher
  28. Khoory J, Estanislau J, Elkhal A, Lazaar A, Melhorn M, Brodsky A, et al. Ligation of Glycophorin A Generates Reactive Oxygen Species Leading to Decreased Red Blood Cell Function. PLoS ONE. 2016;11:e0141206 pubmed publisher
  29. Colonna L, Parry G, Panicker S, Elkon K. Uncoupling complement C1s activation from C1q binding in apoptotic cell phagocytosis and immunosuppressive capacity. Clin Immunol. 2016;163:84-90 pubmed publisher
  30. Sartain S, Turner N, Moake J. TNF Regulates Essential Alternative Complement Pathway Components and Impairs Activation of Protein C in Human Glomerular Endothelial Cells. J Immunol. 2016;196:832-45 pubmed publisher
  31. Neher M, Rich M, Keene C, Weckbach S, Bolden A, Losacco J, et al. Deficiency of complement receptors CR2/CR1 in Cr2?/? mice reduces the extent of secondary brain damage after closed head injury. J Neuroinflammation. 2014;11:95 pubmed publisher
  32. Shi J, Rose E, Singh A, Hussain S, Stagliano N, Parry G, et al. TNT003, an inhibitor of the serine protease C1s, prevents complement activation induced by cold agglutinins. Blood. 2014;123:4015-22 pubmed publisher
  33. Moliva J, Rajaram M, Sidiki S, Sasindran S, Guirado E, Pan X, et al. Molecular composition of the alveolar lining fluid in the aging lung. Age (Dordr). 2014;36:9633 pubmed publisher
  34. Meng H, Zhang L, E X, Ye F, Li H, Han C, et al. Application of Oxford classification, and overexpression of transforming growth factor-?1 and immunoglobulins in immunoglobulin A nephropathy: correlation with World Health Organization classification of immunoglobulin A nephropathy in a Chinese pati. Transl Res. 2014;163:8-18 pubmed publisher
  35. Thomas S, van der Vlies A, O Neil C, Reddy S, Yu S, Giorgio T, et al. Engineering complement activation on polypropylene sulfide vaccine nanoparticles. Biomaterials. 2011;32:2194-203 pubmed publisher
  36. Khodoun M, Strait R, Orekov T, Hogan S, Karasuyama H, Herbert D, et al. Peanuts can contribute to anaphylactic shock by activating complement. J Allergy Clin Immunol. 2009;123:342-51 pubmed publisher