This is a Validated Antibody Database (VAD) review about human BUB1B, based on 23 published articles (read how Labome selects the articles), using BUB1B antibody in all methods. It is aimed to help Labome visitors find the most suited BUB1B antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
BUB1B synonym: BUB1beta; BUBR1; Bub1A; MAD3L; MVA1; SSK1; hBUBR1

Knockout validation
BD Biosciences
mouse monoclonal (9/BUBR1)
  • immunocytochemistry knockout validation; mouse; 1:250; fig 3
  • western blot knockout validation; mouse; 1:1000; fig 6
  • immunoprecipitation; mouse; fig 6
BD Biosciences BUB1B antibody (BD Transduction, 612503) was used in immunocytochemistry knockout validation on mouse samples at 1:250 (fig 3), in western blot knockout validation on mouse samples at 1:1000 (fig 6) and in immunoprecipitation on mouse samples (fig 6). elife (2016) ncbi
Abcam
domestic rabbit monoclonal (EPR12259(2))
  • immunocytochemistry; human; 1:100; loading ...; fig s8d
Abcam BUB1B antibody (Abcam, ab183496) was used in immunocytochemistry on human samples at 1:100 (fig s8d). Proc Natl Acad Sci U S A (2020) ncbi
domestic rabbit monoclonal
  • western blot; human; 1:1000; loading ...; fig 4d
Abcam BUB1B antibody (Abcam, ab200062) was used in western blot on human samples at 1:1000 (fig 4d). Cell Death Differ (2019) ncbi
mouse monoclonal
  • western blot; human; 1:1000; loading ...; fig 4d
Abcam BUB1B antibody (Abcam, ab54894) was used in western blot on human samples at 1:1000 (fig 4d). Cell Death Differ (2019) ncbi
mouse monoclonal
  • immunocytochemistry; mouse; 1:50; fig 4
Abcam BUB1B antibody (Abcam, ab54894) was used in immunocytochemistry on mouse samples at 1:50 (fig 4). Sci Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1c
Abcam BUB1B antibody (Abcam, Ab-70544) was used in western blot on human samples (fig 1c). Cell Cycle (2016) ncbi
mouse monoclonal
  • immunohistochemistry; mouse; 1:200; fig 5
Abcam BUB1B antibody (Abcam, ab54894) was used in immunohistochemistry on mouse samples at 1:200 (fig 5). Aging (Albany NY) (2015) ncbi
Novus Biologicals
mouse monoclonal (8G1)
  • western blot; human
Novus Biologicals BUB1B antibody (NovusBio, NB100-353BR1) was used in western blot on human samples . Nat Commun (2014) ncbi
LifeSpan Biosciences
mouse monoclonal (8G1)
  • immunocytochemistry; human; loading ...; fig 8l
  • western blot; human
LifeSpan Biosciences BUB1B antibody (LifeSpan BioSciences, LS-C2771) was used in immunocytochemistry on human samples (fig 8l) and in western blot on human samples . J Biol Chem (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D32E8)
  • immunohistochemistry; human; 1:200; fig s18
Cell Signaling Technology BUB1B antibody (Cell signaling, 5421) was used in immunohistochemistry on human samples at 1:200 (fig s18). Nat Commun (2015) ncbi
domestic rabbit monoclonal (D32E8)
  • immunocytochemistry; human; 1:100
Cell Signaling Technology BUB1B antibody (Cell Signaling Technology, D32E8) was used in immunocytochemistry on human samples at 1:100. Nat Commun (2014) ncbi
BD Biosciences
mouse monoclonal (9/BUBR1)
  • immunocytochemistry; human; loading ...; fig s2
In order to assess the effects of CCT271850 on MPS1 kinase activity in cancer cells, BD Biosciences BUB1B antibody (BD Bioscience, 612503) was used in immunocytochemistry on human samples (fig s2). Br J Cancer (2017) ncbi
mouse monoclonal (9/BUBR1)
  • immunocytochemistry; human; 1:100; fig 5c
BD Biosciences BUB1B antibody (BD Biosciences, 612503) was used in immunocytochemistry on human samples at 1:100 (fig 5c). Sci Rep (2016) ncbi
mouse monoclonal (9/BUBR1)
  • immunocytochemistry knockout validation; mouse; 1:250; fig 3
  • western blot knockout validation; mouse; 1:1000; fig 6
  • immunoprecipitation; mouse; fig 6
BD Biosciences BUB1B antibody (BD Transduction, 612503) was used in immunocytochemistry knockout validation on mouse samples at 1:250 (fig 3), in western blot knockout validation on mouse samples at 1:1000 (fig 6) and in immunoprecipitation on mouse samples (fig 6). elife (2016) ncbi
mouse monoclonal (9/BUBR1)
  • immunocytochemistry; human; fig 4a
BD Biosciences BUB1B antibody (BD Transduction, 612503) was used in immunocytochemistry on human samples (fig 4a). Exp Mol Med (2016) ncbi
mouse monoclonal (9/BUBR1)
  • ELISA; human; 1:1000; fig 1c
  • western blot; human; 1:1000
BD Biosciences BUB1B antibody (BD Bioscience, BubR1) was used in ELISA on human samples at 1:1000 (fig 1c) and in western blot on human samples at 1:1000. MAbs (2016) ncbi
mouse monoclonal (9/BUBR1)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences BUB1B antibody (BD, 9) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (9/BUBR1)
  • western blot; mouse; loading ...; fig 5b
In order to present the role of nuclear pore protein nup88 on anaphase-promoting complex, BD Biosciences BUB1B antibody (BD Transduction Laboratories, 612503) was used in western blot on mouse samples (fig 5b). J Clin Invest (2016) ncbi
mouse monoclonal (9/BUBR1)
  • immunocytochemistry; human; fig 3
  • western blot; human; fig 2
BD Biosciences BUB1B antibody (BD Transduction Laboratories, 612503) was used in immunocytochemistry on human samples (fig 3) and in western blot on human samples (fig 2). PLoS ONE (2015) ncbi
mouse monoclonal (9/BUBR1)
  • immunocytochemistry; human
In order to study the relationship between CENP-H and kinetochore microtubules, BD Biosciences BUB1B antibody (BD Biosciences, 612503) was used in immunocytochemistry on human samples . J Biol Chem (2015) ncbi
mouse monoclonal (9/BUBR1)
  • western blot; human; fig 1
BD Biosciences BUB1B antibody (BD Biosciences, 612503) was used in western blot on human samples (fig 1). Genetics (2015) ncbi
mouse monoclonal (9/BUBR1)
  • western blot; human; 1:1000; fig s1
BD Biosciences BUB1B antibody (MD Transduction, 612503) was used in western blot on human samples at 1:1000 (fig s1). Nat Commun (2015) ncbi
mouse monoclonal (9/BUBR1)
  • immunocytochemistry; human
  • western blot; human
In order to elucidate the mechanism by which BI2536 induces defects in cell proliferation of non-small cell lung cancer, BD Biosciences BUB1B antibody (bd, 612503) was used in immunocytochemistry on human samples and in western blot on human samples . Cancer Lett (2015) ncbi
mouse monoclonal (9/BUBR1)
  • immunoprecipitation; human; fig 3
BD Biosciences BUB1B antibody (BD transduction laboratories, 612503) was used in immunoprecipitation on human samples (fig 3). Nature (2015) ncbi
mouse monoclonal (9/BUBR1)
  • immunocytochemistry; human; 1:1000
BD Biosciences BUB1B antibody (BD Biosciences, 612503) was used in immunocytochemistry on human samples at 1:1000. FEBS Lett (2014) ncbi
mouse monoclonal (9/BUBR1)
  • western blot; human; fig 2b
In order to report that nuclear pore complexes in interphase cells function as scaffolds for anaphase-inhibitory signaling, BD Biosciences BUB1B antibody (BD, 612503) was used in western blot on human samples (fig 2b). Cell (2014) ncbi
mouse monoclonal (9/BUBR1)
  • western blot; human
BD Biosciences BUB1B antibody (BD biosciences, 612503) was used in western blot on human samples . Cell Cycle (2014) ncbi
Articles Reviewed
  1. Nielsen C, Zhang T, Barisic M, Kalitsis P, Hudson D. Topoisomerase IIα is essential for maintenance of mitotic chromosome structure. Proc Natl Acad Sci U S A. 2020;117:12131-12142 pubmed publisher
  2. Frank T, Tuppi M, Hugle M, Dötsch V, van Wijk S, Fulda S. Cell cycle arrest in mitosis promotes interferon-induced necroptosis. Cell Death Differ. 2019;: pubmed publisher
  3. Faisal A, Mak G, Gurden M, Xavier C, Anderhub S, Innocenti P, et al. Characterisation of CCT271850, a selective, oral and potent MPS1 inhibitor, used to directly measure in vivo MPS1 inhibition vs therapeutic efficacy. Br J Cancer. 2017;116:1166-1176 pubmed publisher
  4. Ikawa Yoshida A, Matsumoto T, Okano S, Aoyagi Y, Matsubara Y, Furuyama T, et al. BubR1 Insufficiency Impairs Liver Regeneration in Aged Mice after Hepatectomy through Intercalated Disc Abnormality. Sci Rep. 2016;6:32399 pubmed publisher
  5. Weaver R, Limzerwala J, Naylor R, Jeganathan K, Baker D, van Deursen J. BubR1 alterations that reinforce mitotic surveillance act against aneuploidy and cancer. elife. 2016;5: pubmed publisher
  6. Park Y, Nam H, Do M, Lee J. The p90 ribosomal S6 kinase 2 specifically affects mitotic progression by regulating the basal level, distribution and stability of mitotic spindles. Exp Mol Med. 2016;48:e250 pubmed publisher
  7. Yuan Y, Ren Y, Yuan P, Yan L, Qiao J. TRAIP is involved in chromosome alignment and SAC regulation in mouse oocyte meiosis. Sci Rep. 2016;6:29735 pubmed publisher
  8. Chung H, Park J, Lee N, Kim H, Jang C. Phosphorylation of Astrin Regulates Its Kinetochore Function. J Biol Chem. 2016;291:17579-92 pubmed publisher
  9. Salsi V, Fantini S, Zappavigna V. NUP98 fusion oncoproteins interact with the APC/C(Cdc20) as a pseudosubstrate and prevent mitotic checkpoint complex binding. Cell Cycle. 2016;15:2275-87 pubmed publisher
  10. Sedgwick G, Larsen M, Lischetti T, Streicher W, Jersie Christensen R, Olsen J, et al. Conformation-specific anti-Mad2 monoclonal antibodies for the dissection of checkpoint signaling. MAbs. 2016;8:689-97 pubmed publisher
  11. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  12. Naylor R, Jeganathan K, Cao X, van Deursen J. Nuclear pore protein NUP88 activates anaphase-promoting complex to promote aneuploidy. J Clin Invest. 2016;126:543-59 pubmed publisher
  13. Ohashi A, Ohori M, Iwai K, Nambu T, Miyamoto M, Kawamoto T, et al. A Novel Time-Dependent CENP-E Inhibitor with Potent Antitumor Activity. PLoS ONE. 2015;10:e0144675 pubmed publisher
  14. Papaconstantinou J, Wang C, Zhang M, Yang S, DeFord J, Bulavin D, et al. Attenuation of p38α MAPK stress response signaling delays the in vivo aging of skeletal muscle myofibers and progenitor cells. Aging (Albany NY). 2015;7:718-33 pubmed
  15. Zhu L, Wang Z, Wang W, Wang C, Hua S, Su Z, et al. Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis. J Biol Chem. 2015;290:27053-66 pubmed publisher
  16. Bailey M, Singh T, Mero P, Moffat J, Hieter P. Dependence of Human Colorectal Cells Lacking the FBW7 Tumor Suppressor on the Spindle Assembly Checkpoint. Genetics. 2015;201:885-95 pubmed publisher
  17. Ohashi A, Ohori M, Iwai K, Nakayama Y, Nambu T, Morishita D, et al. Aneuploidy generates proteotoxic stress and DNA damage concurrently with p53-mediated post-mitotic apoptosis in SAC-impaired cells. Nat Commun. 2015;6:7668 pubmed publisher
  18. Choi M, Kim W, Cheon M, Lee C, Kim J. Polo-like kinase 1 inhibitor BI2536 causes mitotic catastrophe following activation of the spindle assembly checkpoint in non-small cell lung cancer cells. Cancer Lett. 2015;357:591-601 pubmed publisher
  19. Brownlow N, Pike T, Zicha D, Collinson L, Parker P. Mitotic catenation is monitored and resolved by a PKCε-regulated pathway. Nat Commun. 2014;5:5685 pubmed publisher
  20. Izawa D, Pines J. The mitotic checkpoint complex binds a second CDC20 to inhibit active APC/C. Nature. 2015;517:631-4 pubmed publisher
  21. Silva P, Reis R, Bolanos Garcia V, Florindo C, Tavares A, Bousbaa H. Dynein-dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles. FEBS Lett. 2014;588:3265-73 pubmed publisher
  22. Rodriguez Bravo V, Maciejowski J, Corona J, Buch H, Collin P, Kanemaki M, et al. Nuclear pores protect genome integrity by assembling a premitotic and Mad1-dependent anaphase inhibitor. Cell. 2014;156:1017-31 pubmed publisher
  23. Singh A, Zapata M, Choi Y, Yoon S. GSI promotes vincristine-induced apoptosis by enhancing multi-polar spindle formation. Cell Cycle. 2014;13:157-66 pubmed publisher