This is a Validated Antibody Database (VAD) review about human BTN3A1, based on 5 published articles (read how Labome selects the articles), using BTN3A1 antibody in all methods. It is aimed to help Labome visitors find the most suited BTN3A1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
BTN3A1 synonym: BT3.1; BTF5; BTN3.1; CD277; butyrophilin subfamily 3 member A1; dJ45P21.3 (butyrophilin, subfamily 3, member A1)

BioLegend
mouse monoclonal (BT3.1)
  • flow cytometry; human; loading ...; fig 4b
In order to analyze the capacity of monocytes, neutrophils, and CD4 T cells to serve as accessory cells for gamma delta T cell activation, BioLegend BTN3A1 antibody (BioLegend, BT3.1) was used in flow cytometry on human samples (fig 4b). J Immunol (2016) ncbi
mouse monoclonal (BT3.1)
  • flow cytometry; human; loading ...; fig 3d
BioLegend BTN3A1 antibody (BioLegend, BT3.1) was used in flow cytometry on human samples (fig 3d). J Immunol (2016) ncbi
Proteintech Group
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 5a
  • western blot; human; 1:500; loading ...; fig 5d
Proteintech Group BTN3A1 antibody (Proteintech, 25221-1-AP) was used in immunoprecipitation on human samples (fig 5a) and in western blot on human samples at 1:500 (fig 5d). Nat Commun (2017) ncbi
Invitrogen
mouse monoclonal (eBioBT3.1 (20.1, BT3.1))
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, Invitrogen BTN3A1 antibody (e- Bioscienc e, 14-2779-71) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (eBioBT3.1 (20.1, BT3.1))
  • flow cytometry; human
  • immunohistochemistry; human; loading ...; fig 5
In order to discuss the role of CD277 in ovarian cancer, Invitrogen BTN3A1 antibody (eBioscience, eBioBT3.1) was used in flow cytometry on human samples and in immunohistochemistry on human samples (fig 5). Oncotarget (2010) ncbi
Articles Reviewed
  1. Castella B, Kopecka J, Sciancalepore P, Mandili G, Foglietta M, Mitro N, et al. The ATP-binding cassette transporter A1 regulates phosphoantigen release and V?9V?2 T cell activation by dendritic cells. Nat Commun. 2017;8:15663 pubmed publisher
  2. Nerdal P, Peters C, Oberg H, Zlatev H, Lettau M, Quabius E, et al. Butyrophilin 3A/CD277-Dependent Activation of Human ?? T Cells: Accessory Cell Capacity of Distinct Leukocyte Populations. J Immunol. 2016;197:3059-3068 pubmed
  3. Kilcollins A, Li J, Hsiao C, Wiemer A. HMBPP Analog Prodrugs Bypass Energy-Dependent Uptake To Promote Efficient BTN3A1-Mediated Malignant Cell Lysis by V?9V?2 T Lymphocyte Effectors. J Immunol. 2016;197:419-28 pubmed publisher
  4. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  5. Cubillos Ruiz J, Martinez D, Scarlett U, Rutkowski M, Nesbeth Y, Camposeco Jacobs A, et al. CD277 is a negative co-stimulatory molecule universally expressed by ovarian cancer microenvironmental cells. Oncotarget. 2010;1:329-38 pubmed