This is a Validated Antibody Database (VAD) review about human BTK, based on 27 published articles (read how Labome selects the articles), using BTK antibody in all methods. It is aimed to help Labome visitors find the most suited BTK antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
BTK synonym: AGMX1; AT; ATK; BPK; IGHD3; IMD1; PSCTK1; XLA

Knockout validation
Abcam
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 5a
Abcam BTK antibody (Abcam, ab25971) was used in western blot knockout validation on mouse samples at 1:1000 (fig 5a). Int J Mol Med (2021) ncbi
BD Biosciences
mouse monoclonal (53/BTK)
  • western blot knockout validation; mouse; fig s2
BD Biosciences BTK antibody (BD Biosciences, 558528) was used in western blot knockout validation on mouse samples (fig s2). J Immunol (2015) ncbi
Abcam
domestic rabbit polyclonal
  • immunocytochemistry; mouse; loading ...
Abcam BTK antibody (Abcam, ab52192) was used in immunocytochemistry on mouse samples . Clin Transl Med (2022) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 5a
Abcam BTK antibody (Abcam, ab25971) was used in western blot knockout validation on mouse samples at 1:1000 (fig 5a). Int J Mol Med (2021) ncbi
domestic rabbit monoclonal (EP420Y)
  • western blot; human
Abcam BTK antibody (Abcam, ab68217) was used in western blot on human samples . Cell Death Dis (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E-9)
  • immunocytochemistry; human; 1:400; fig 3
  • western blot; human; 1:700; fig 2
Santa Cruz Biotechnology BTK antibody (Santa Cruz, sc-28387) was used in immunocytochemistry on human samples at 1:400 (fig 3) and in western blot on human samples at 1:700 (fig 2). Oncotarget (2015) ncbi
mouse monoclonal (7F12H4)
  • immunohistochemistry - paraffin section; human; 1:400; fig 1
  • western blot; human; 1:600; fig 1
Santa Cruz Biotechnology BTK antibody (Santa Cruz, SC-81159) was used in immunohistochemistry - paraffin section on human samples at 1:400 (fig 1) and in western blot on human samples at 1:600 (fig 1). Oncotarget (2015) ncbi
OriGene
mouse monoclonal (OTI10E10)
  • flow cytometry; human; 2 ug/ml; loading ...; fig 3a
OriGene BTK antibody (OriGene Technologies, 10E10) was used in flow cytometry on human samples at 2 ug/ml (fig 3a). J Hematol Oncol (2016) ncbi
Invitrogen
mouse monoclonal (7F12H4)
  • immunohistochemistry; human; 1:20,000; tbl 2
In order to evaluate the reliance on EBV or NF-kappaB signaling instead of B-cell receptor signaling in regards to complete phenotypic characterization of PTLD, Invitrogen BTK antibody (Thermo, MA5-15337) was used in immunohistochemistry on human samples at 1:20,000 (tbl 2). Hematol Oncol (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 2d
Cell Signaling Technology BTK antibody (Cell Signaling, 5082) was used in western blot on mouse samples (fig 2d). Leukemia (2022) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; mouse; loading ...; fig 2d
Cell Signaling Technology BTK antibody (Cell Signaling, 8547) was used in western blot on mouse samples (fig 2d). Leukemia (2022) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; mouse; loading ...; fig 2h
Cell Signaling Technology BTK antibody (Cell Signaling Technology, 8547S) was used in western blot on mouse samples (fig 2h). Clin Transl Med (2022) ncbi
domestic rabbit monoclonal (D3H5)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1b
Cell Signaling Technology BTK antibody (Cell Signaling Technology, 8547) was used in immunohistochemistry on mouse samples at 1:200 (fig 1b). Int J Mol Med (2021) ncbi
domestic rabbit monoclonal (D3H5)
  • mass cytometry; human; 1:200; loading ...; fig s2
Cell Signaling Technology BTK antibody (Cell Signaling, D3H5) was used in mass cytometry on human samples at 1:200 (fig s2). Int J Mol Sci (2021) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; human; 1:1000; loading ...; fig 3b, 3e
Cell Signaling Technology BTK antibody (Cell Signaling and Technology, D3H5) was used in western blot on human samples at 1:1000 (fig 3b, 3e). Mol Oncol (2020) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; mouse; loading ...; fig s1a
Cell Signaling Technology BTK antibody (Cell Signaling, 8547S) was used in western blot on mouse samples (fig s1a). Oncogene (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig s1a
Cell Signaling Technology BTK antibody (Cell Signaling, 5082S) was used in western blot on mouse samples (fig s1a). Oncogene (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 6a
Cell Signaling Technology BTK antibody (Cell Signaling, 5082S) was used in western blot on human samples at 1:1000 (fig 6a). Gut (2018) ncbi
domestic rabbit monoclonal (C82B8)
  • western blot; human; loading ...; fig 3f
Cell Signaling Technology BTK antibody (Cell Signaling, 3533) was used in western blot on human samples (fig 3f). Oncotarget (2017) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; human; loading ...; fig 5d
In order to report that therapeutic antibody-dependent tumor cell killing was FcgammaR-dependent and mediated by macrophages, Cell Signaling Technology BTK antibody (Cell Signalling, 8547) was used in western blot on human samples (fig 5d). Oncogene (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 5d
In order to report that therapeutic antibody-dependent tumor cell killing was FcgammaR-dependent and mediated by macrophages, Cell Signaling Technology BTK antibody (Cell Signalling, 5082) was used in western blot on human samples (fig 5d). Oncogene (2017) ncbi
domestic rabbit monoclonal (C82B8)
  • western blot; human
Cell Signaling Technology BTK antibody (Cell Signaling, 3533) was used in western blot on human samples . Nature (2016) ncbi
domestic rabbit monoclonal (C82B8)
  • western blot; mouse; 1:1000; fig 4
Cell Signaling Technology BTK antibody (Cell Signaling, 3533) was used in western blot on mouse samples at 1:1000 (fig 4). Leukemia (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 5b
In order to elucidate how hepatitis C virus infection alters B cell proliferation, Cell Signaling Technology BTK antibody (cell signalling technology, 5082) was used in western blot on human samples (fig 5b). Oncogene (2016) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; human; fig 5b
In order to elucidate how hepatitis C virus infection alters B cell proliferation, Cell Signaling Technology BTK antibody (cell signalling technology, 8547) was used in western blot on human samples (fig 5b). Oncogene (2016) ncbi
domestic rabbit monoclonal (C82B8)
  • western blot; human; 1:1000; fig 5
Cell Signaling Technology BTK antibody (Cell Signaling Tech, 3533) was used in western blot on human samples at 1:1000 (fig 5). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; rat; fig 5
Cell Signaling Technology BTK antibody (Cell Signaling, 8547) was used in western blot on rat samples (fig 5). Nat Chem Biol (2015) ncbi
domestic rabbit monoclonal (C82B8)
  • western blot; human; fig 1a
  • western blot; mouse; fig 2b
Cell Signaling Technology BTK antibody (Cell Signaling, 3533) was used in western blot on human samples (fig 1a) and in western blot on mouse samples (fig 2b). Nature (2015) ncbi
domestic rabbit monoclonal (D3H5)
  • western blot; mouse; 1:1000; fig 8c
In order to explore a macrophage TLR9-BTK-calcineurin-NFAT signaling pathway involved in impair fungal immunity, Cell Signaling Technology BTK antibody (Cell signaling, 8547) was used in western blot on mouse samples at 1:1000 (fig 8c). EMBO Mol Med (2015) ncbi
domestic rabbit monoclonal (C82B8)
  • western blot; mouse
Cell Signaling Technology BTK antibody (Cell Signaling Technology, 3533) was used in western blot on mouse samples . Molecules (2014) ncbi
BD Biosciences
mouse monoclonal (N35-86)
  • immunohistochemistry; mouse; loading ...; fig s2b
BD Biosciences BTK antibody (BD, 564848) was used in immunohistochemistry on mouse samples (fig s2b). Oncogene (2019) ncbi
mouse monoclonal (N35-86)
  • flow cytometry; mouse; loading ...; fig 6e
BD Biosciences BTK antibody (BD Biosciences, 564848) was used in flow cytometry on mouse samples (fig 6e). Cell Rep (2018) ncbi
mouse monoclonal (N35-86)
  • flow cytometry; mouse; loading ...; fig 1f
BD Biosciences BTK antibody (BD Biosciences, N35-86) was used in flow cytometry on mouse samples (fig 1f). Front Immunol (2018) ncbi
mouse monoclonal (N35-86)
  • flow cytometry; human; loading ...; fig 1b
BD Biosciences BTK antibody (BD, 562753) was used in flow cytometry on human samples (fig 1b). J Exp Med (2018) ncbi
mouse monoclonal (24a/BTK)
BD Biosciences BTK antibody (BD Biosciences, 558034) was used . Sci Rep (2016) ncbi
mouse monoclonal (53/BTK)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences BTK antibody (BD, 53) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (24a/BTK)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences BTK antibody (BD, 24A/BTK) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (53/BTK)
  • western blot knockout validation; mouse; fig s2
BD Biosciences BTK antibody (BD Biosciences, 558528) was used in western blot knockout validation on mouse samples (fig s2). J Immunol (2015) ncbi
mouse monoclonal (53/BTK)
  • western blot; human; 1:500; fig 3
BD Biosciences BTK antibody (BD Biosciences, 611117) was used in western blot on human samples at 1:500 (fig 3). Nat Chem Biol (2015) ncbi
Articles Reviewed
  1. Lee A, Pingali S, Pinilla Ibarz J, Atchison M, Koumenis C, Argon Y, et al. Loss of AID exacerbates the malignant progression of CLL. Leukemia. 2022;36:2430-2442 pubmed publisher
  2. Zhu Y, Gu H, Yang L, Li N, Chen Q, Kang D, et al. Involvement of MST1/mTORC1/STAT1 activity in the regulation of B-cell receptor signalling by chemokine receptor 2. Clin Transl Med. 2022;12:e887 pubmed publisher
  3. Zhao J, Chen J, Li Y, Xia L, Wu Y. Bruton's tyrosine kinase regulates macrophage‑induced inflammation in the diabetic kidney via NLRP3 inflammasome activation. Int J Mol Med. 2021;48: pubmed publisher
  4. West J, Austin E, Rizzi E, Yan L, Tanjore H, Crabtree A, et al. KCNK3 Mutation Causes Altered Immune Function in Pulmonary Arterial Hypertension Patients and Mouse Models. Int J Mol Sci. 2021;22: pubmed publisher
  5. Thomsen E, Rovsing A, Anderson M, Due H, Huang J, Luo Y, et al. Identification of BLNK and BTK as mediators of rituximab-induced programmed cell death by CRISPR screens in GCB-subtype diffuse large B-cell lymphoma. Mol Oncol. 2020;: pubmed publisher
  6. Das S, Bar Sagi D. BTK signaling drives CD1dhiCD5+ regulatory B-cell differentiation to promote pancreatic carcinogenesis. Oncogene. 2019;38:3316-3324 pubmed publisher
  7. Obino D, Fetler L, Soza A, Malbec O, Saez J, Labarca M, et al. Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse. Cell Rep. 2018;25:3110-3122.e6 pubmed publisher
  8. Hwang I, Boularan C, Harrison K, Kehrl J. Gαi Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression. Front Immunol. 2018;9:687 pubmed publisher
  9. Tissino E, Benedetti D, Herman S, ten Hacken E, Ahn I, Chaffee K, et al. Functional and clinical relevance of VLA-4 (CD49d/CD29) in ibrutinib-treated chronic lymphocytic leukemia. J Exp Med. 2018;215:681-697 pubmed publisher
  10. Chong I, Aronson L, Bryant H, Gulati A, Campbell J, Elliott R, et al. Mapping genetic vulnerabilities reveals BTK as a novel therapeutic target in oesophageal cancer. Gut. 2018;67:1780-1792 pubmed publisher
  11. Mondello P, Derenzini E, Asgari Z, Philip J, Brea E, SESHAN V, et al. Dual inhibition of histone deacetylases and phosphoinositide 3-kinase enhances therapeutic activity against B cell lymphoma. Oncotarget. 2017;8:14017-14028 pubmed publisher
  12. Burgess M, Mapp S, Mazzieri R, Cheung C, Chambers L, Mattarollo S, et al. Increased FcγRIIB dominance contributes to the emergence of resistance to therapeutic antibodies in chronic lymphocytic leukaemia patients. Oncogene. 2017;36:2366-2376 pubmed publisher
  13. Liu C, Richard K, Wiggins M, Zhu X, Conrad D, Song W. CD23 can negatively regulate B-cell receptor signaling. Sci Rep. 2016;6:25629 pubmed publisher
  14. Ikegame K, Imai K, Yamashita M, Hoshino A, Kanegane H, Morio T, et al. Allogeneic stem cell transplantation for X-linked agammaglobulinemia using reduced intensity conditioning as a model of the reconstitution of humoral immunity. J Hematol Oncol. 2016;9:9 pubmed publisher
  15. He Y, Zeng M, Yang D, Motro B, Núñez G. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. Nature. 2016;530:354-7 pubmed publisher
  16. Menter T, Dickenmann M, Juskevicius D, Steiger J, Dirnhofer S, Tzankov A. Comprehensive phenotypic characterization of PTLD reveals potential reliance on EBV or NF-κB signalling instead of B-cell receptor signalling. Hematol Oncol. 2017;35:187-197 pubmed publisher
  17. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  18. Chen S, Chang B, Chang S, Tong T, Ham S, Sherry B, et al. BTK inhibition results in impaired CXCR4 chemokine receptor surface expression, signaling and function in chronic lymphocytic leukemia. Leukemia. 2016;30:833-43 pubmed publisher
  19. Dai B, Chen A, Corkum C, Peroutka R, Landon A, Houng S, et al. Hepatitis C virus upregulates B-cell receptor signaling: a novel mechanism for HCV-associated B-cell lymphoproliferative disorders. Oncogene. 2016;35:2979-90 pubmed publisher
  20. Lorkova L, Scigelova M, Arrey T, Vit O, Pospisilova J, Doktorova E, et al. Detailed Functional and Proteomic Characterization of Fludarabine Resistance in Mantle Cell Lymphoma Cells. PLoS ONE. 2015;10:e0135314 pubmed publisher
  21. Zucha M, Wu A, Lee W, Wang L, Lin W, Yuan C, et al. Bruton's tyrosine kinase (Btk) inhibitor ibrutinib suppresses stem-like traits in ovarian cancer. Oncotarget. 2015;6:13255-68 pubmed
  22. Tampella G, Kerns H, Niu D, Singh S, Khim S, Bosch K, et al. The Tec Kinase-Regulated Phosphoproteome Reveals a Mechanism for the Regulation of Inhibitory Signals in Murine Macrophages. J Immunol. 2015;195:246-56 pubmed publisher
  23. Bradshaw J, McFarland J, Paavilainen V, Bisconte A, Tam D, Phan V, et al. Prolonged and tunable residence time using reversible covalent kinase inhibitors. Nat Chem Biol. 2015;11:525-31 pubmed publisher
  24. Chen Z, Shojaee S, Buchner M, Geng H, Lee J, Klemm L, et al. Signalling thresholds and negative B-cell selection in acute lymphoblastic leukaemia. Nature. 2015;521:357-61 pubmed publisher
  25. Herbst S, Shah A, Mazon Moya M, Marzola V, Jensen B, Reed A, et al. Phagocytosis-dependent activation of a TLR9-BTK-calcineurin-NFAT pathway co-ordinates innate immunity to Aspergillus fumigatus. EMBO Mol Med. 2015;7:240-58 pubmed publisher
  26. Guo W, Liu R, Bhardwaj G, Yang J, Changou C, Ma A, et al. Targeting Btk/Etk of prostate cancer cells by a novel dual inhibitor. Cell Death Dis. 2014;5:e1409 pubmed publisher
  27. Baek J, Kim J, Cheon Y, Park S, Ahn S, Yoon K, et al. Aconitum pseudo-laeve var. erectum inhibits receptor activator of nuclear factor kappa-B ligand-induced osteoclastogenesis via the c-Fos/nuclear factor of activated T-cells, cytoplasmic 1 signaling pathway and prevents lipopolysaccharide-induced bone. Molecules. 2014;19:11628-44 pubmed publisher