This is a Validated Antibody Database (VAD) review about human BST2, based on 20 published articles (read how Labome selects the articles), using BST2 antibody in all methods. It is aimed to help Labome visitors find the most suited BST2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
BST2 synonym: CD317; TETHERIN

Knockout validation
Abnova
mouse polyclonal
  • western blot knockout validation; human; fig 3
In order to test if tetherin attaches to exosomes, Abnova BST2 antibody (Abnova, B02P) was used in western blot knockout validation on human samples (fig 3). elife (2016) ncbi
BioLegend
mouse monoclonal (RS38E)
  • other; human; loading ...; fig 7s1b
BioLegend BST2 antibody (BioLegend, RS38E) was used in other on human samples (fig 7s1b). elife (2019) ncbi
mouse monoclonal (RS38E)
  • flow cytometry; human; loading ...; fig 1s2
BioLegend BST2 antibody (BioLegend, 348405) was used in flow cytometry on human samples (fig 1s2). elife (2019) ncbi
mouse monoclonal (RS38E)
  • flow cytometry; human; 1:20; loading ...; fig 1d
BioLegend BST2 antibody (Biolegend, 348410) was used in flow cytometry on human samples at 1:20 (fig 1d). elife (2019) ncbi
mouse monoclonal (RS38E)
  • flow cytometry; human; loading ...; fig 1a
BioLegend BST2 antibody (BioLegend, RS38E) was used in flow cytometry on human samples (fig 1a). PLoS Pathog (2018) ncbi
mouse monoclonal (RS38E)
  • flow cytometry; human; loading ...; fig 4b
BioLegend BST2 antibody (BioLegend, 348410) was used in flow cytometry on human samples (fig 4b). J Virol (2018) ncbi
mouse monoclonal (RS38E)
  • western blot; human; 1:100; loading ...; fig 1c
In order to investigate mechanisms that mediate BST2 turnover, BioLegend BST2 antibody (BioLegend, RS38E) was used in western blot on human samples at 1:100 (fig 1c). J Cell Sci (2017) ncbi
mouse monoclonal (RS38E)
In order to analyze HIV-1 Vpu sequences isolated from patients, BioLegend BST2 antibody (Biolegend, 348410) was used . Sci Rep (2017) ncbi
mouse monoclonal (RS38E)
  • flow cytometry; human; fig 8c
BioLegend BST2 antibody (Biolegend, RS38E) was used in flow cytometry on human samples (fig 8c). PLoS Pathog (2017) ncbi
mouse monoclonal (RS38E)
  • flow cytometry; human; fig 2
BioLegend BST2 antibody (BioLegend, 348410) was used in flow cytometry on human samples (fig 2). Traffic (2016) ncbi
mouse monoclonal (RS38E)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BioLegend BST2 antibody (BioLegen d, 348404) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
mouse monoclonal (RS38E)
  • flow cytometry; human; 1:25; fig 3
In order to discuss the HIV rev1-vpu gene fusion, BioLegend BST2 antibody (BioLegend, 348410) was used in flow cytometry on human samples at 1:25 (fig 3). PLoS ONE (2015) ncbi
mouse monoclonal (RS38E)
  • flow cytometry; human; fig s4
BioLegend BST2 antibody (BioLegend, 348406) was used in flow cytometry on human samples (fig s4). Stem Cell Reports (2015) ncbi
Invitrogen
mouse monoclonal (26F8)
  • western blot; human; 1:500; loading ...; fig 5b
Invitrogen BST2 antibody (eBioscience, 16-3179-82) was used in western blot on human samples at 1:500 (fig 5b). J Virol (2018) ncbi
mouse monoclonal (26F8)
  • immunocytochemistry; human; fig 6
Invitrogen BST2 antibody (eBiosciences, 26F8) was used in immunocytochemistry on human samples (fig 6). PLoS Pathog (2015) ncbi
mouse monoclonal (26F8)
  • flow cytometry; human; 1:200; fig s4
Invitrogen BST2 antibody (eBiosciences, 26F8) was used in flow cytometry on human samples at 1:200 (fig s4). Nat Commun (2015) ncbi
mouse monoclonal (26F8)
  • flow cytometry; human; fig s4
Invitrogen BST2 antibody (eBioscience, 12-3179) was used in flow cytometry on human samples (fig s4). Stem Cell Reports (2015) ncbi
mouse monoclonal (26F8)
  • flow cytometry; human; fig 1
Invitrogen BST2 antibody (eBioscience, 12-3179) was used in flow cytometry on human samples (fig 1). Virology (2015) ncbi
Abcam
domestic rabbit monoclonal (EPR20202-150)
  • flow cytometry; human; fig 2g
Abcam BST2 antibody (Abcam, ab243230) was used in flow cytometry on human samples (fig 2g). Cell Stem Cell (2022) ncbi
Abnova
mouse monoclonal (2.00E+06)
  • immunocytochemistry; human; 1:750; loading ...; fig 5a
In order to investigate mechanisms that mediate BST2 turnover, Abnova BST2 antibody (Abnova, 2E6) was used in immunocytochemistry on human samples at 1:750 (fig 5a). J Cell Sci (2017) ncbi
mouse polyclonal
  • western blot knockout validation; human; fig 3
In order to test if tetherin attaches to exosomes, Abnova BST2 antibody (Abnova, B02P) was used in western blot knockout validation on human samples (fig 3). elife (2016) ncbi
mouse monoclonal (2.00E+06)
  • immunocytochemistry; human; 1:200; fig 3
Abnova BST2 antibody (Abnova, H00000684-M15) was used in immunocytochemistry on human samples at 1:200 (fig 3). Virol J (2016) ncbi
mouse polyclonal
  • western blot; Human immunodeficiency virus 1; loading ...; fig 2b
In order to report that guanylate binding protein 5 potently restricts HIV-1 and other retroviruses, Abnova BST2 antibody (Abnova, H00000684-B02P) was used in western blot on Human immunodeficiency virus 1 samples (fig 2b). Cell Host Microbe (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E-4)
  • western blot; human; fig 1c
Santa Cruz Biotechnology BST2 antibody (SantaCruz, sc-390719) was used in western blot on human samples (fig 1c). Cell (2018) ncbi
Articles Reviewed
  1. Pham T, Panda A, Kagawa H, To S, Ertekin C, Georgolopoulos G, et al. Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells. Cell Stem Cell. 2022;29:1346-1365.e10 pubmed publisher
  2. Saliba D, Céspedes Donoso P, Balint S, Compeer E, Korobchevskaya K, Valvo S, et al. Composition and structure of synaptic ectosomes exporting antigen receptor linked to functional CD40 ligand from helper T cells. elife. 2019;8: pubmed publisher
  3. Naamati A, Williamson J, Greenwood E, Marelli S, Lehner P, Matheson N. Functional proteomic atlas of HIV infection in primary human CD4+ T cells. elife. 2019;8: pubmed publisher
  4. Langer S, Hammer C, Hopfensperger K, Klein L, Hotter D, De Jesus P, et al. HIV-1 Vpu is a potent transcriptional suppressor of NF-κB-elicited antiviral immune responses. elife. 2019;8: pubmed publisher
  5. Urata S, Kenyon E, Nayak D, Cubitt B, Kurosaki Y, Yasuda J, et al. BST-2 controls T cell proliferation and exhaustion by shaping the early distribution of a persistent viral infection. PLoS Pathog. 2018;14:e1007172 pubmed publisher
  6. Heusinger E, Deppe K, Sette P, Krapp C, Kmiec D, Kluge S, et al. Preadaptation of Simian Immunodeficiency Virus SIVsmm Facilitated Env-Mediated Counteraction of Human Tetherin by Human Immunodeficiency Virus Type 2. J Virol. 2018;92: pubmed publisher
  7. Wu X, Dao Thi V, Huang Y, Billerbeck E, Saha D, Hoffmann H, et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell. 2018;172:423-438.e25 pubmed publisher
  8. Roy N, Pacini G, Berlioz Torrent C, Janvier K. Characterization of E3 ligases involved in lysosomal sorting of the HIV-1 restriction factor BST2. J Cell Sci. 2017;130:1596-1611 pubmed publisher
  9. Romani B, Kavyanifard A, Allahbakhshi E. Functional conservation and coherence of HIV-1 subtype A Vpu alleles. Sci Rep. 2017;7:44894 pubmed publisher
  10. Yoder A, Guo K, Dillon S, Phang T, Lee E, Harper M, et al. The transcriptome of HIV-1 infected intestinal CD4+ T cells exposed to enteric bacteria. PLoS Pathog. 2017;13:e1006226 pubmed publisher
  11. Edgar J, Manna P, Nishimura S, Banting G, Robinson M. Tetherin is an exosomal tether. elife. 2016;5: pubmed publisher
  12. Liu Y, Li M, Zhang D, Zhang M, Hu Q. HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway. Virol J. 2016;13:154 pubmed publisher
  13. Rapiteanu R, Davis L, Williamson J, Timms R, Paul Luzio J, Lehner P. A Genetic Screen Identifies a Critical Role for the WDR81-WDR91 Complex in the Trafficking and Degradation of Tetherin. Traffic. 2016;17:940-58 pubmed publisher
  14. Krapp C, Hotter D, Gawanbacht A, McLaren P, Kluge S, Stürzel C, et al. Guanylate Binding Protein (GBP) 5 Is an Interferon-Inducible Inhibitor of HIV-1 Infectivity. Cell Host Microbe. 2016;19:504-14 pubmed publisher
  15. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  16. Langer S, Hopfensperger K, Iyer S, Kreider E, Learn G, Lee L, et al. A Naturally Occurring rev1-vpu Fusion Gene Does Not Confer a Fitness Advantage to HIV-1. PLoS ONE. 2015;10:e0142118 pubmed publisher
  17. Bego M, Côté Ã, Aschman N, Mercier J, Weissenhorn W, Cohen Ã. Vpu Exploits the Cross-Talk between BST2 and the ILT7 Receptor to Suppress Anti-HIV-1 Responses by Plasmacytoid Dendritic Cells. PLoS Pathog. 2015;11:e1005024 pubmed publisher
  18. Adoro S, Cubillos Ruiz J, Chen X, Deruaz M, Vrbanac V, Song M, et al. IL-21 induces antiviral microRNA-29 in CD4 T cells to limit HIV-1 infection. Nat Commun. 2015;6:7562 pubmed publisher
  19. James S, Fox J, Afsari F, Lee J, Clough S, Knight C, et al. Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes. Stem Cell Reports. 2015;4:1004-15 pubmed publisher
  20. Liu Y, Luo S, He S, Zhang M, Wang P, Li C, et al. Tetherin restricts HSV-2 release and is counteracted by multiple viral glycoproteins. Virology. 2015;475:96-109 pubmed publisher