This is a Validated Antibody Database (VAD) review about human BRD4, based on 26 published articles (read how Labome selects the articles), using BRD4 antibody in all methods. It is aimed to help Labome visitors find the most suited BRD4 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
BRD4 synonym: CAP; HUNK1; HUNKI; MCAP

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (E2A7X)
  • western blot knockout validation; human; loading ...; fig s2b
Cell Signaling Technology BRD4 antibody (Cell Signaling, 13440S) was used in western blot knockout validation on human samples (fig s2b). Nature (2017) ncbi
Abcam
domestic rabbit monoclonal (EPR5150(2))
  • immunohistochemistry - frozen section; human; loading ...; fig 1b, 1c
Abcam BRD4 antibody (Abcam, ab128874) was used in immunohistochemistry - frozen section on human samples (fig 1b, 1c). Science (2020) ncbi
domestic rabbit monoclonal (EPR5150(2))
  • western blot; human; fig 1c
Abcam BRD4 antibody (Abcam, ab128874) was used in western blot on human samples (fig 1c). Cell Res (2018) ncbi
domestic rabbit monoclonal (EPR5150(2))
  • immunoprecipitation; human; loading ...; fig 1b
  • western blot; human; loading ...; fig 4a
Abcam BRD4 antibody (Abcam, ab128874) was used in immunoprecipitation on human samples (fig 1b) and in western blot on human samples (fig 4a). Nat Med (2017) ncbi
domestic rabbit monoclonal (EPR5150(2))
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 6d
  • western blot; mouse; 1:1000; loading ...; fig 7b
Abcam BRD4 antibody (Abcam, 128874) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 6d) and in western blot on mouse samples at 1:1000 (fig 7b). J Neuroinflammation (2017) ncbi
domestic rabbit monoclonal (EPR5150(2))
  • immunoprecipitation; human; loading ...; fig 4b
  • immunocytochemistry; human; 1:500; loading ...; fig 5b
  • western blot; human; loading ...; fig 3a
Abcam BRD4 antibody (Abcam, ab128874) was used in immunoprecipitation on human samples (fig 4b), in immunocytochemistry on human samples at 1:500 (fig 5b) and in western blot on human samples (fig 3a). PLoS Pathog (2016) ncbi
domestic rabbit monoclonal (EPR5150(2))
  • immunohistochemistry; human; 1:100; fig s2
  • western blot; human; 1:1000; fig s1
Abcam BRD4 antibody (Abcam, ab128874) was used in immunohistochemistry on human samples at 1:100 (fig s2) and in western blot on human samples at 1:1000 (fig s1). Mol Cancer Ther (2016) ncbi
domestic rabbit monoclonal (EPR5150(2))
  • ChIP-Seq; mouse; fig 5
  • western blot; mouse; fig 3b
Abcam BRD4 antibody (Abcam, 128874) was used in ChIP-Seq on mouse samples (fig 5) and in western blot on mouse samples (fig 3b). BMC Genomics (2016) ncbi
domestic rabbit monoclonal (EPR5150(2))
  • immunocytochemistry; human; 1:200; fig 4
Abcam BRD4 antibody (Abcam, ab128874) was used in immunocytochemistry on human samples at 1:200 (fig 4). Epigenetics Chromatin (2015) ncbi
domestic rabbit monoclonal (EPR5150(2))
  • western blot; mouse; fig s1f
Abcam BRD4 antibody (abcam, ab128874) was used in western blot on mouse samples (fig s1f). Nature (2015) ncbi
domestic rabbit monoclonal (EPR5150(2))
  • western blot; human; fig 3
In order to assess reduction of c-MYC in acute leukemia cells due to a BET inhibitor OTX015 that targets BRD2 and BRD4, Abcam BRD4 antibody (Epitomics, 5716-1) was used in western blot on human samples (fig 3). Oncotarget (2015) ncbi
domestic rabbit monoclonal (EPR5150(2))
  • western blot; human; 1:5000; fig s1
Abcam BRD4 antibody (Epitomics, 5716-1) was used in western blot on human samples at 1:5000 (fig s1). Cell Rep (2014) ncbi
domestic rabbit monoclonal (EPR5150(2))
  • western blot; mouse
Abcam BRD4 antibody (Abcam, ab128874) was used in western blot on mouse samples . In Vivo (2014) ncbi
Active Motif
domestic rabbit polyclonal
  • western blot; human; 1:200; loading ...; fig 1b
In order to ask if the small-molecule inhibitor JQ1 in combination with romidepsin serves as a novel therapeutic option for type II testicular germ cell cancers, Active Motif BRD4 antibody (Active Motif, 39909) was used in western blot on human samples at 1:200 (fig 1b). J Cell Mol Med (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (E2A7X)
  • western blot; human; loading ...; fig 5a
Cell Signaling Technology BRD4 antibody (Bethyl Laboratories, 13440) was used in western blot on human samples (fig 5a). Sci Adv (2020) ncbi
domestic rabbit monoclonal (E2A7X)
  • western blot; human; loading ...; fig 5a
Cell Signaling Technology BRD4 antibody (Cell Signaling Technology, 13440) was used in western blot on human samples (fig 5a). Mod Pathol (2020) ncbi
domestic rabbit monoclonal (E2A7X)
  • chromatin immunoprecipitation; human; fig 6d
Cell Signaling Technology BRD4 antibody (Cell Signaling, 13440) was used in chromatin immunoprecipitation on human samples (fig 6d). Cancer Cell (2018) ncbi
domestic rabbit monoclonal (E2A7X)
  • other; human; loading ...; fig 4c
Cell Signaling Technology BRD4 antibody (Cell Signaling, 13440) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit monoclonal (E2A7X)
  • chromatin immunoprecipitation; human; loading ...; fig s2b
Cell Signaling Technology BRD4 antibody (Cell Signalling, E2A7X) was used in chromatin immunoprecipitation on human samples (fig s2b). Science (2017) ncbi
domestic rabbit monoclonal (E2A7X)
  • western blot; human; 1:1000; loading ...; fig 1j
Cell Signaling Technology BRD4 antibody (Cell Signaling Technology, 13440S) was used in western blot on human samples at 1:1000 (fig 1j). Mol Cell (2017) ncbi
domestic rabbit monoclonal (E2A7X)
  • western blot knockout validation; human; loading ...; fig s2b
Cell Signaling Technology BRD4 antibody (Cell Signaling, 13440S) was used in western blot knockout validation on human samples (fig s2b). Nature (2017) ncbi
domestic rabbit monoclonal (E2A7X)
  • western blot; human; loading ...; fig s3d
Cell Signaling Technology BRD4 antibody (CST, 13440) was used in western blot on human samples (fig s3d). Proc Natl Acad Sci U S A (2017) ncbi
domestic rabbit monoclonal (E2A7X)
Cell Signaling Technology BRD4 antibody (Cell Signaling, E2A7X) was used . Nat Struct Mol Biol (2016) ncbi
domestic rabbit monoclonal (E2A7X)
  • western blot; human; fig 2
Cell Signaling Technology BRD4 antibody (Cell Signaling, 13440) was used in western blot on human samples (fig 2). Proc Natl Acad Sci U S A (2016) ncbi
domestic rabbit monoclonal (E2A7X)
  • immunoprecipitation; human; loading ...; fig 2c
  • western blot; human; loading ...; fig s1
Cell Signaling Technology BRD4 antibody (Cell Signaling, E2A7X) was used in immunoprecipitation on human samples (fig 2c) and in western blot on human samples (fig s1). Oncotarget (2016) ncbi
domestic rabbit monoclonal (E2A7X)
  • chromatin immunoprecipitation; human; loading ...; fig 4c
  • western blot; human; loading ...; fig 6e
Cell Signaling Technology BRD4 antibody (Cell Signaling, 13440) was used in chromatin immunoprecipitation on human samples (fig 4c) and in western blot on human samples (fig 6e). Oncotarget (2016) ncbi
domestic rabbit monoclonal (E2A7X)
  • western blot; human; 1:1000; fig 3h
Cell Signaling Technology BRD4 antibody (Cell Signaling Technology, 13440) was used in western blot on human samples at 1:1000 (fig 3h). Nat Commun (2016) ncbi
Articles Reviewed
  1. Klein I, Boija A, Afeyan L, Hawken S, Fan M, Dall Agnese A, et al. Partitioning of cancer therapeutics in nuclear condensates. Science. 2020;368:1386-1392 pubmed publisher
  2. Reynders M, Matsuura B, Bérouti M, Simoneschi D, Marzio A, Pagano M, et al. PHOTACs enable optical control of protein degradation. Sci Adv. 2020;6:eaay5064 pubmed publisher
  3. Basturk O, Weigelt B, Adsay V, Benhamida J, Askan G, Wang L, et al. Sclerosing epithelioid mesenchymal neoplasm of the pancreas - a proposed new entity. Mod Pathol. 2020;33:456-467 pubmed publisher
  4. Yu F, Shi G, Cheng S, Chen J, Wu S, Wang Z, et al. SUMO suppresses and MYC amplifies transcription globally by regulating CDK9 sumoylation. Cell Res. 2018;28:670-685 pubmed publisher
  5. Andricovich J, Perkail S, Kai Y, Casasanta N, Peng W, Tzatsos A. Loss of KDM6A Activates Super-Enhancers to Induce Gender-Specific Squamous-like Pancreatic Cancer and Confers Sensitivity to BET Inhibitors. Cancer Cell. 2018;33:512-526.e8 pubmed publisher
  6. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  7. Zhang P, Wang D, Zhao Y, Ren S, Gao K, Ye Z, et al. Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation. Nat Med. 2017;23:1055-1062 pubmed publisher
  8. Tyler D, Vappiani J, Cañeque T, Lam E, Ward A, Gilan O, et al. Click chemistry enables preclinical evaluation of targeted epigenetic therapies. Science. 2017;356:1397-1401 pubmed publisher
  9. Sakamaki J, Wilkinson S, Hahn M, Tasdemir N, O Prey J, Clark W, et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell. 2017;66:517-532.e9 pubmed publisher
  10. Golden R, Chen B, Li T, Braun J, Manjunath H, Chen X, et al. An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Nature. 2017;542:197-202 pubmed publisher
  11. Zhao L, Li J, Fu Y, Zhang M, Wang B, Ouellette J, et al. Photoreceptor protection via blockade of BET epigenetic readers in a murine model of inherited retinal degeneration. J Neuroinflammation. 2017;14:14 pubmed publisher
  12. Sherman M, Yu R, Tseng T, Sousa C, Liu S, Truitt M, et al. Stromal cues regulate the pancreatic cancer epigenome and metabolome. Proc Natl Acad Sci U S A. 2017;114:1129-1134 pubmed publisher
  13. Jostes S, Nettersheim D, Fellermeyer M, Schneider S, Hafezi F, Honecker F, et al. The bromodomain inhibitor JQ1 triggers growth arrest and apoptosis in testicular germ cell tumours in vitro and in vivo. J Cell Mol Med. 2017;21:1300-1314 pubmed publisher
  14. Ren K, Zhang W, Chen X, Ma Y, Dai Y, Fan Y, et al. An Epigenetic Compound Library Screen Identifies BET Inhibitors That Promote HSV-1 and -2 Replication by Bridging P-TEFb to Viral Gene Promoters through BRD4. PLoS Pathog. 2016;12:e1005950 pubmed publisher
  15. Gilan O, Lam E, Becher I, Lugo D, Cannizzaro E, Joberty G, et al. Functional interdependence of BRD4 and DOT1L in MLL leukemia. Nat Struct Mol Biol. 2016;23:673-81 pubmed publisher
  16. Raina K, Lu J, Qian Y, Altieri M, Gordon D, Rossi A, et al. PROTAC-induced BET protein degradation as a therapy for castration-resistant prostate cancer. Proc Natl Acad Sci U S A. 2016;113:7124-9 pubmed publisher
  17. Blee A, Liu S, Wang L, Huang H. BET bromodomain-mediated interaction between ERG and BRD4 promotes prostate cancer cell invasion. Oncotarget. 2016;7:38319-38332 pubmed publisher
  18. Hong S, Eun J, Choi S, Shen Q, Choi W, Han J, et al. Epigenetic reader BRD4 inhibition as a therapeutic strategy to suppress E2F2-cell cycle regulation circuit in liver cancer. Oncotarget. 2016;7:32628-40 pubmed publisher
  19. Tögel L, Nightingale R, Chueh A, Jayachandran A, Tran H, Phesse T, et al. Dual Targeting of Bromodomain and Extraterminal Domain Proteins, and WNT or MAPK Signaling, Inhibits c-MYC Expression and Proliferation of Colorectal Cancer Cells. Mol Cancer Ther. 2016;15:1217-26 pubmed publisher
  20. Shao Z, Zhang R, Khodadadi Jamayran A, Chen B, Crowley M, Festok M, et al. The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016;7:10869 pubmed publisher
  21. Gonzales Cope M, Sidoli S, Bhanu N, Won K, Garcia B. Histone H4 acetylation and the epigenetic reader Brd4 are critical regulators of pluripotency in embryonic stem cells. BMC Genomics. 2016;17:95 pubmed publisher
  22. Zhan Y, Kost Alimova M, Shi X, Leo E, Bardenhagen J, Shepard H, et al. Development of novel cellular histone-binding and chromatin-displacement assays for bromodomain drug discovery. Epigenetics Chromatin. 2015;8:37 pubmed publisher
  23. Fong C, Gilan O, Lam E, Rubin A, Ftouni S, Tyler D, et al. BET inhibitor resistance emerges from leukaemia stem cells. Nature. 2015;525:538-42 pubmed publisher
  24. Coudé M, Braun T, Berrou J, Dupont M, Bertrand S, Massé A, et al. BET inhibitor OTX015 targets BRD2 and BRD4 and decreases c-MYC in acute leukemia cells. Oncotarget. 2015;6:17698-712 pubmed
  25. Nagarajan S, Hossan T, Alawi M, Najafova Z, Indenbirken D, Bedi U, et al. Bromodomain protein BRD4 is required for estrogen receptor-dependent enhancer activation and gene transcription. Cell Rep. 2014;8:460-9 pubmed publisher
  26. Kalash R, Berhane H, Au J, Rhieu B, Epperly M, Goff J, et al. Differences in irradiated lung gene transcription between fibrosis-prone C57BL/6NHsd and fibrosis-resistant C3H/HeNHsd mice. In Vivo. 2014;28:147-71 pubmed