This is a Validated Antibody Database (VAD) review about human BRD3, based on 15 published articles (read how Labome selects the articles), using BRD3 antibody in all methods. It is aimed to help Labome visitors find the most suited BRD3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
BRD3 synonym: ORFX; RING3L; bromodomain-containing protein 3; RING3-like protein; bromodomain-containing protein 3 short isoform

Bethyl
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; loading ...; fig 3d
  • immunoprecipitation; human; loading ...; fig 1b
  • western blot; human; loading ...; fig 4a
Bethyl BRD3 antibody (Bethyl, A302-368A) was used in immunohistochemistry - paraffin section on human samples (fig 3d), in immunoprecipitation on human samples (fig 1b) and in western blot on human samples (fig 4a). Nat Med (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig s5b
Bethyl BRD3 antibody (Bethyl, A302-368A) was used in western blot on human samples (fig s5b). Science (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:100; fig s2
Bethyl BRD3 antibody (Bethyl Laboratories, A302-368A) was used in immunohistochemistry on human samples at 1:100 (fig s2). Mol Cancer Ther (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 1
Bethyl BRD3 antibody (Bethyl Laboratories, A302-368A) was used in western blot on mouse samples (fig 1). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 3
In order to study triple-negative breast cancer and BET bromodomain inhibitor response and resistance, Bethyl BRD3 antibody (Bethyl, A302-368A) was used in western blot on human samples (fig 3). Nature (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
Bethyl BRD3 antibody (Bethyl Laboratories, A310-859A) was used in chromatin immunoprecipitation on human samples . Proc Natl Acad Sci U S A (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:500; fig 1a
In order to report that an inhibitor of the bromodomain and extraterminal family of proteins suppresses pancreatic ductal adenocarcinoma, Bethyl BRD3 antibody (Bethyl Laboratories, a302-367) was used in western blot on human samples at 1:500 (fig 1a). Nat Med (2015) ncbi
rabbit polyclonal
  • ChIP-Seq; mouse; fig s7
Bethyl BRD3 antibody (Bethyl Labs, A302-368A) was used in ChIP-Seq on mouse samples (fig s7). Nature (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse
Bethyl BRD3 antibody (Bethyl Laboratories, A302-367A) was used in chromatin immunoprecipitation on mouse samples . Nucleic Acids Res (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (2088C3a)
  • western blot; human; loading ...; fig s3d
Santa Cruz Biotechnology BRD3 antibody (SCBT, sc-81202) was used in western blot on human samples (fig s3d). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (2088C3a)
  • western blot; human; fig 2
Santa Cruz Biotechnology BRD3 antibody (Santa Cruz, sc-81202) was used in western blot on human samples (fig 2). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (2088C3a)
  • western blot; human
Santa Cruz Biotechnology BRD3 antibody (Santa, sc-81202) was used in western blot on human samples . Nature (2014) ncbi
Abcam
mouse monoclonal (2088C3a)
  • western blot; human; 1:50; loading ...; fig 1b
In order to ask if the small-molecule inhibitor JQ1 in combination with romidepsin serves as a novel therapeutic option for type II testicular germ cell cancers, Abcam BRD3 antibody (Abcam, ab50818) was used in western blot on human samples at 1:50 (fig 1b). J Cell Mol Med (2017) ncbi
mouse monoclonal (2088C3a)
  • western blot; human; 1:50; fig s1
Abcam BRD3 antibody (Abcam, ab50818) was used in western blot on human samples at 1:50 (fig s1). Mol Cancer Ther (2016) ncbi
Invitrogen
mouse monoclonal
  • immunoprecipitation; human; 2 ug
In order to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality, Invitrogen BRD3 antibody (life tech, 730116) was used in immunoprecipitation on human samples at 2 ug. Nat Methods (2015) ncbi
mouse monoclonal
  • immunoprecipitation; human; 2 ug
In order to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality, Invitrogen BRD3 antibody (life tech, 730024) was used in immunoprecipitation on human samples at 2 ug. Nat Methods (2015) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; human; 1:1000; fig 4d
Proteintech Group BRD3 antibody (Proteintech, 11859-1-AP) was used in western blot on human samples at 1:1000 (fig 4d). Nat Commun (2016) ncbi
Articles Reviewed
  1. Zhang P, Wang D, Zhao Y, Ren S, Gao K, Ye Z, et al. Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation. Nat Med. 2017;23:1055-1062 pubmed publisher
  2. Tyler D, Vappiani J, Cañeque T, Lam E, Ward A, Gilan O, et al. Click chemistry enables preclinical evaluation of targeted epigenetic therapies. Science. 2017;356:1397-1401 pubmed publisher
  3. Sherman M, Yu R, Tseng T, Sousa C, Liu S, Truitt M, et al. Stromal cues regulate the pancreatic cancer epigenome and metabolome. Proc Natl Acad Sci U S A. 2017;114:1129-1134 pubmed publisher
  4. Jostes S, Nettersheim D, Fellermeyer M, Schneider S, Hafezi F, Honecker F, et al. The bromodomain inhibitor JQ1 triggers growth arrest and apoptosis in testicular germ cell tumours in vitro and in vivo. J Cell Mol Med. 2017;21:1300-1314 pubmed publisher
  5. Raina K, Lu J, Qian Y, Altieri M, Gordon D, Rossi A, et al. PROTAC-induced BET protein degradation as a therapy for castration-resistant prostate cancer. Proc Natl Acad Sci U S A. 2016;113:7124-9 pubmed publisher
  6. Tögel L, Nightingale R, Chueh A, Jayachandran A, Tran H, Phesse T, et al. Dual Targeting of Bromodomain and Extraterminal Domain Proteins, and WNT or MAPK Signaling, Inhibits c-MYC Expression and Proliferation of Colorectal Cancer Cells. Mol Cancer Ther. 2016;15:1217-26 pubmed publisher
  7. Shao Z, Zhang R, Khodadadi Jamayran A, Chen B, Crowley M, Festok M, et al. The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016;7:10869 pubmed publisher
  8. Nakagawa A, Adams C, Huang Y, Hamarneh S, Liu W, Von Alt K, et al. Selective and reversible suppression of intestinal stem cell differentiation by pharmacological inhibition of BET bromodomains. Sci Rep. 2016;6:20390 pubmed publisher
  9. Shu S, Lin C, He H, Witwicki R, Tabassum D, Roberts J, et al. Response and resistance to BET bromodomain inhibitors in triple-negative breast cancer. Nature. 2016;529:413-417 pubmed publisher
  10. Ding N, Hah N, Yu R, Sherman M, Benner C, Leblanc M, et al. BRD4 is a novel therapeutic target for liver fibrosis. Proc Natl Acad Sci U S A. 2015;112:15713-8 pubmed publisher
  11. Mazur P, Herner A, Mello S, Wirth M, Hausmann S, Sánchez Rivera F, et al. Combined inhibition of BET family proteins and histone deacetylases as a potential epigenetics-based therapy for pancreatic ductal adenocarcinoma. Nat Med. 2015;21:1163-71 pubmed publisher
  12. Fong C, Gilan O, Lam E, Rubin A, Ftouni S, Tyler D, et al. BET inhibitor resistance emerges from leukaemia stem cells. Nature. 2015;525:538-42 pubmed publisher
  13. Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, et al. Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation. Nat Methods. 2015;12:725-31 pubmed publisher
  14. Hu Y, Belaghzal H, Hsiao W, Qi J, Bradner J, Guertin D, et al. Transcriptional and post-transcriptional control of adipocyte differentiation by Jumonji domain-containing protein 6. Nucleic Acids Res. 2015;43:7790-804 pubmed publisher
  15. Asangani I, Dommeti V, Wang X, Malik R, Cieslik M, Yang R, et al. Therapeutic targeting of BET bromodomain proteins in castration-resistant prostate cancer. Nature. 2014;510:278-82 pubmed publisher