This is a Validated Antibody Database (VAD) review about human BRD2, based on 24 published articles (read how Labome selects the articles), using BRD2 antibody in all methods. It is aimed to help Labome visitors find the most suited BRD2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
BRD2 synonym: BRD2-IT1; D6S113E; FSH; FSRG1; NAT; O27.1.1; RING3; RNF3; bromodomain-containing protein 2; BRD2 intronic transcript 1; female sterile homeotic-related gene 1; really interesting new gene 3 protein

Bethyl
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; loading ...; fig 3d
Bethyl BRD2 antibody (Bethyl, A302-583A) was used in immunohistochemistry - paraffin section on human samples (fig 3d). Nat Med (2017) ncbi
rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig 1c
  • chromatin immunoprecipitation; mouse; loading ...; fig 1d
  • immunoprecipitation; mouse; loading ...; fig 2d
  • western blot; mouse; loading ...; fig 6c
In order to explore the contributions of Brd2 and Brd4 during mouse T helper 17 cell differentiation, Bethyl BRD2 antibody (Bethyl Laboratories, IHC-00612) was used in ChIP-Seq on mouse samples (fig 1c), in chromatin immunoprecipitation on mouse samples (fig 1d), in immunoprecipitation on mouse samples (fig 2d) and in western blot on mouse samples (fig 6c). Mol Cell (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 7a
Bethyl BRD2 antibody (Bethyl laboratories, A302-583A) was used in western blot on mouse samples (fig 7a). J Neuroinflammation (2017) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; mouse; fig s1
  • immunoprecipitation; mouse; fig s1
  • western blot; mouse; 1:2000; fig s1
Bethyl BRD2 antibody (Bethyl, A302-583A) was used in chromatin immunoprecipitation on mouse samples (fig s1), in immunoprecipitation on mouse samples (fig s1) and in western blot on mouse samples at 1:2000 (fig s1). BMC Mol Biol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 1
Bethyl BRD2 antibody (Bethyl Laboratories, A302-583A) was used in western blot on mouse samples (fig 1). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 3
In order to study triple-negative breast cancer and BET bromodomain inhibitor response and resistance, Bethyl BRD2 antibody (Bethyl, A302-583A) was used in western blot on human samples (fig 3). Nature (2016) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human
Bethyl BRD2 antibody (Bethyl Laboratories, A302-583A) was used in chromatin immunoprecipitation on human samples . Proc Natl Acad Sci U S A (2015) ncbi
rabbit polyclonal
  • ChIP-Seq; mouse; fig s7
Bethyl BRD2 antibody (Bethyl Labs, A302-583A) was used in ChIP-Seq on mouse samples (fig s7). Nature (2015) ncbi
rabbit polyclonal
Bethyl BRD2 antibody (Bethyl Laboratories, A302?C583A) was used . Nucleic Acids Res (2015) ncbi
rabbit polyclonal
  • chromatin immunoprecipitation; human; fig 4,5,6
Bethyl BRD2 antibody (Bethyl, A302-583A) was used in chromatin immunoprecipitation on human samples (fig 4,5,6). Blood (2015) ncbi
rabbit polyclonal
Bethyl BRD2 antibody (Montgomery, A302-583A) was used . Mol Cancer Ther (2014) ncbi
Abcam
rabbit monoclonal (EPR7642)
  • immunoprecipitation; human; loading ...; fig 1b
  • western blot; human; loading ...; fig 4a
Abcam BRD2 antibody (Abcam, ab139690) was used in immunoprecipitation on human samples (fig 1b) and in western blot on human samples (fig 4a). Nat Med (2017) ncbi
rabbit monoclonal (EPR7642)
  • immunohistochemistry - paraffin section; mouse; 1:150
  • western blot; mouse; 1:1000; loading ...; fig 7a
Abcam BRD2 antibody (Abcam, ab139690) was used in immunohistochemistry - paraffin section on mouse samples at 1:150 and in western blot on mouse samples at 1:1000 (fig 7a). J Neuroinflammation (2017) ncbi
rabbit monoclonal (EPR7642)
  • western blot; human; loading ...; fig 2a
Abcam BRD2 antibody (Abcam, ab139690) was used in western blot on human samples (fig 2a). Oncotarget (2017) ncbi
LifeSpan Biosciences
rabbit polyclonal
  • western blot; mouse; fig 1,2,3,4
In order to study mammalian postmeiotic sperm development and BRD4, LifeSpan Biosciences BRD2 antibody (life span biosciences, LS-B923) was used in western blot on mouse samples (fig 1,2,3,4). Mol Cell Biol (2015) ncbi
Invitrogen
mouse monoclonal
  • immunoprecipitation; human; 2 ug
In order to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality, Invitrogen BRD2 antibody (life tech, 730115) was used in immunoprecipitation on human samples at 2 ug. Nat Methods (2015) ncbi
Abnova
rabbit polyclonal
  • western blot; human
Abnova BRD2 antibody (Abnova, PAB3245) was used in western blot on human samples . Nature (2014) ncbi
Cell Signaling Technology
rabbit monoclonal (D89B4)
  • western blot; human; loading ...; fig s5b
Cell Signaling Technology BRD2 antibody (Cell Signalling, D89B4) was used in western blot on human samples (fig s5b). Science (2017) ncbi
rabbit monoclonal (D89B4)
  • western blot; human; loading ...; fig s3d
Cell Signaling Technology BRD2 antibody (CST, 5848) was used in western blot on human samples (fig s3d). Proc Natl Acad Sci U S A (2017) ncbi
rabbit monoclonal (D89B4)
  • western blot; human; fig 2
Cell Signaling Technology BRD2 antibody (Cell Signaling, 5848) was used in western blot on human samples (fig 2). Proc Natl Acad Sci U S A (2016) ncbi
rabbit monoclonal (D89B4)
  • immunohistochemistry; human; 1:100; fig s2
Cell Signaling Technology BRD2 antibody (Cell Signalling Technology, 5848) was used in immunohistochemistry on human samples at 1:100 (fig s2). Mol Cancer Ther (2016) ncbi
rabbit monoclonal (D89B4)
  • western blot; human; 1:1000; fig 3h
Cell Signaling Technology BRD2 antibody (Cell Signaling Technology, 5848) was used in western blot on human samples at 1:1000 (fig 3h). Nat Commun (2016) ncbi
rabbit monoclonal (D89B4)
  • western blot; human; 1:500; fig 6
Cell Signaling Technology BRD2 antibody (Cell Signaling, 5848) was used in western blot on human samples at 1:500 (fig 6). Nature (2015) ncbi
rabbit monoclonal (D89B4)
  • western blot; mouse; 1:1000; fig 1a
In order to report that an inhibitor of the bromodomain and extraterminal family of proteins suppresses pancreatic ductal adenocarcinoma, Cell Signaling Technology BRD2 antibody (Cell Signaling Technology, 5848) was used in western blot on mouse samples at 1:1000 (fig 1a). Nat Med (2015) ncbi
rabbit monoclonal (D89B4)
  • chromatin immunoprecipitation; human; fig 8
Cell Signaling Technology BRD2 antibody (Cell Signaling Tech, 5848) was used in chromatin immunoprecipitation on human samples (fig 8). Nucleic Acids Res (2015) ncbi
rabbit monoclonal (D89B4)
  • ChIP-Seq; human
Cell Signaling Technology BRD2 antibody (Cell signaling, 5848) was used in ChIP-Seq on human samples . Nat Biotechnol (2014) ncbi
Articles Reviewed
  1. Zhang P, Wang D, Zhao Y, Ren S, Gao K, Ye Z, et al. Intrinsic BET inhibitor resistance in SPOP-mutated prostate cancer is mediated by BET protein stabilization and AKT-mTORC1 activation. Nat Med. 2017;23:1055-1062 pubmed publisher
  2. Tyler D, Vappiani J, Cañeque T, Lam E, Ward A, Gilan O, et al. Click chemistry enables preclinical evaluation of targeted epigenetic therapies. Science. 2017;356:1397-1401 pubmed publisher
  3. Cheung K, Zhang F, Jaganathan A, Sharma R, Zhang Q, Konuma T, et al. Distinct Roles of Brd2 and Brd4 in Potentiating the Transcriptional Program for Th17 Cell Differentiation. Mol Cell. 2017;65:1068-1080.e5 pubmed publisher
  4. Zhao L, Li J, Fu Y, Zhang M, Wang B, Ouellette J, et al. Photoreceptor protection via blockade of BET epigenetic readers in a murine model of inherited retinal degeneration. J Neuroinflammation. 2017;14:14 pubmed publisher
  5. Sherman M, Yu R, Tseng T, Sousa C, Liu S, Truitt M, et al. Stromal cues regulate the pancreatic cancer epigenome and metabolome. Proc Natl Acad Sci U S A. 2017;114:1129-1134 pubmed publisher
  6. Vázquez R, Riveiro M, Astorgues Xerri L, Odore E, Rezai K, Erba E, et al. The bromodomain inhibitor OTX015 (MK-8628) exerts anti-tumor activity in triple-negative breast cancer models as single agent and in combination with everolimus. Oncotarget. 2017;8:7598-7613 pubmed publisher
  7. Raina K, Lu J, Qian Y, Altieri M, Gordon D, Rossi A, et al. PROTAC-induced BET protein degradation as a therapy for castration-resistant prostate cancer. Proc Natl Acad Sci U S A. 2016;113:7124-9 pubmed publisher
  8. Pinz S, Unser S, Rascle A. Signal transducer and activator of transcription STAT5 is recruited to c-Myc super-enhancer. BMC Mol Biol. 2016;17:10 pubmed publisher
  9. Tögel L, Nightingale R, Chueh A, Jayachandran A, Tran H, Phesse T, et al. Dual Targeting of Bromodomain and Extraterminal Domain Proteins, and WNT or MAPK Signaling, Inhibits c-MYC Expression and Proliferation of Colorectal Cancer Cells. Mol Cancer Ther. 2016;15:1217-26 pubmed publisher
  10. Shao Z, Zhang R, Khodadadi Jamayran A, Chen B, Crowley M, Festok M, et al. The acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016;7:10869 pubmed publisher
  11. Nakagawa A, Adams C, Huang Y, Hamarneh S, Liu W, Von Alt K, et al. Selective and reversible suppression of intestinal stem cell differentiation by pharmacological inhibition of BET bromodomains. Sci Rep. 2016;6:20390 pubmed publisher
  12. Shu S, Lin C, He H, Witwicki R, Tabassum D, Roberts J, et al. Response and resistance to BET bromodomain inhibitors in triple-negative breast cancer. Nature. 2016;529:413-417 pubmed publisher
  13. Ding N, Hah N, Yu R, Sherman M, Benner C, Leblanc M, et al. BRD4 is a novel therapeutic target for liver fibrosis. Proc Natl Acad Sci U S A. 2015;112:15713-8 pubmed publisher
  14. Werner A, Iwasaki S, McGourty C, Medina Ruiz S, Teerikorpi N, Fedrigo I, et al. Cell-fate determination by ubiquitin-dependent regulation of translation. Nature. 2015;525:523-7 pubmed publisher
  15. Mazur P, Herner A, Mello S, Wirth M, Hausmann S, Sánchez Rivera F, et al. Combined inhibition of BET family proteins and histone deacetylases as a potential epigenetics-based therapy for pancreatic ductal adenocarcinoma. Nat Med. 2015;21:1163-71 pubmed publisher
  16. Fong C, Gilan O, Lam E, Rubin A, Ftouni S, Tyler D, et al. BET inhibitor resistance emerges from leukaemia stem cells. Nature. 2015;525:538-42 pubmed publisher
  17. Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, et al. Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation. Nat Methods. 2015;12:725-31 pubmed publisher
  18. Hu Y, Belaghzal H, Hsiao W, Qi J, Bradner J, Guertin D, et al. Transcriptional and post-transcriptional control of adipocyte differentiation by Jumonji domain-containing protein 6. Nucleic Acids Res. 2015;43:7790-804 pubmed publisher
  19. Chan S, Selth L, Li Y, Nyquist M, Miao L, Bradner J, et al. Targeting chromatin binding regulation of constitutively active AR variants to overcome prostate cancer resistance to endocrine-based therapies. Nucleic Acids Res. 2015;43:5880-97 pubmed publisher
  20. Stonestrom A, Hsu S, Jahn K, Huang P, Keller C, Giardine B, et al. Functions of BET proteins in erythroid gene expression. Blood. 2015;125:2825-34 pubmed publisher
  21. Bryant J, Donahue G, Wang X, Meyer Ficca M, Luense L, Weller A, et al. Characterization of BRD4 during mammalian postmeiotic sperm development. Mol Cell Biol. 2015;35:1433-48 pubmed publisher
  22. Asangani I, Dommeti V, Wang X, Malik R, Cieslik M, Yang R, et al. Therapeutic targeting of BET bromodomain proteins in castration-resistant prostate cancer. Nature. 2014;510:278-82 pubmed publisher
  23. Liu S, Walker S, Nelson E, Cerulli R, Xiang M, Toniolo P, et al. Targeting STAT5 in hematologic malignancies through inhibition of the bromodomain and extra-terminal (BET) bromodomain protein BRD2. Mol Cancer Ther. 2014;13:1194-205 pubmed publisher
  24. Anders L, Guenther M, Qi J, Fan Z, Marineau J, Rahl P, et al. Genome-wide localization of small molecules. Nat Biotechnol. 2014;32:92-6 pubmed publisher