This is a Validated Antibody Database (VAD) review about human BMI1, based on 27 published articles (read how Labome selects the articles), using BMI1 antibody in all methods. It is aimed to help Labome visitors find the most suited BMI1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
BMI1 synonym: FLVI2/BMI1; PCGF4; RNF51; flvi-2/bmi-1

Knockout validation
Abcam
domestic rabbit monoclonal (EPR3745(2))
  • western blot knockout validation; human; loading ...; fig 2d
  • immunohistochemistry - paraffin section; human; loading ...; fig 1c
  • immunoprecipitation; human; loading ...; fig 5a
Abcam BMI1 antibody (Abcam, ab126783) was used in western blot knockout validation on human samples (fig 2d), in immunohistochemistry - paraffin section on human samples (fig 1c) and in immunoprecipitation on human samples (fig 5a). Aging (Albany NY) (2021) ncbi
Abcam
domestic rabbit monoclonal (EPR3745(2))
  • western blot knockout validation; human; loading ...; fig 2d
  • immunohistochemistry - paraffin section; human; loading ...; fig 1c
  • immunoprecipitation; human; loading ...; fig 5a
Abcam BMI1 antibody (Abcam, ab126783) was used in western blot knockout validation on human samples (fig 2d), in immunohistochemistry - paraffin section on human samples (fig 1c) and in immunoprecipitation on human samples (fig 5a). Aging (Albany NY) (2021) ncbi
domestic rabbit monoclonal (EPR3745(2))
  • western blot; human; fig EV4
Abcam BMI1 antibody (Abcam, ab126783) was used in western blot on human samples (fig EV4). EMBO Rep (2016) ncbi
domestic rabbit monoclonal (EPR3745(2))
  • immunohistochemistry - paraffin section; human; loading ...; fig 6c
  • western blot; human; loading ...; fig 1a
Abcam BMI1 antibody (Abcam, ab126783) was used in immunohistochemistry - paraffin section on human samples (fig 6c) and in western blot on human samples (fig 1a). Oncotarget (2015) ncbi
domestic rabbit monoclonal (EPR3745(2))
  • flow cytometry; human
Abcam BMI1 antibody (Abcam, ab126783) was used in flow cytometry on human samples . Cell Death Dis (2013) ncbi
Abnova
mouse monoclonal (3E3)
  • immunohistochemistry; human; loading ...; fig 7c
Abnova BMI1 antibody (Abnova, MAB10506) was used in immunohistochemistry on human samples (fig 7c). Oncotarget (2015) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D42B3)
  • other; human; 1:100; loading ...; fig s7a
Cell Signaling Technology BMI1 antibody (Cell Signaling, 5856) was used in other on human samples at 1:100 (fig s7a). Nat Commun (2021) ncbi
domestic rabbit monoclonal (D20B7)
  • western blot; human; 1:1000; loading ...; fig 6a, 6b
Cell Signaling Technology BMI1 antibody (Cell Signaling Technologies, 6964 S) was used in western blot on human samples at 1:1000 (fig 6a, 6b). Commun Biol (2021) ncbi
domestic rabbit monoclonal (D20B7)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1c
  • western blot; human; 1:1000; loading ...; fig 2a, 2b
Cell Signaling Technology BMI1 antibody (CST, 6964) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 1c) and in western blot on human samples at 1:1000 (fig 2a, 2b). Mol Oncol (2021) ncbi
domestic rabbit monoclonal (D20B7)
  • western blot; mouse; 1:1000; loading ...; fig 3c
Cell Signaling Technology BMI1 antibody (CST, 6964S) was used in western blot on mouse samples at 1:1000 (fig 3c). JCI Insight (2020) ncbi
domestic rabbit monoclonal (D20B7)
  • immunocytochemistry; human; loading ...; fig 3a
Cell Signaling Technology BMI1 antibody (Cell Signaling Technology, 6964) was used in immunocytochemistry on human samples (fig 3a). Nature (2020) ncbi
domestic rabbit monoclonal (D20B7)
  • western blot; mouse; loading ...; fig 5c
Cell Signaling Technology BMI1 antibody (Cell Signaling, 6964) was used in western blot on mouse samples (fig 5c). Biomed Pharmacother (2020) ncbi
domestic rabbit monoclonal (D20B7)
  • western blot; human; loading ...; fig 5a
Cell Signaling Technology BMI1 antibody (Cell Signaling Technology, 6964) was used in western blot on human samples (fig 5a). Cancer Res (2019) ncbi
domestic rabbit monoclonal (D20B7)
  • western blot; human; loading ...; fig 5a
Cell Signaling Technology BMI1 antibody (Cell Signaling, 6964) was used in western blot on human samples (fig 5a). J Exp Clin Cancer Res (2019) ncbi
domestic rabbit monoclonal (D42B3)
  • western blot; human; loading ...; fig 3a
Cell Signaling Technology BMI1 antibody (Cell Signaling Technology, 5856) was used in western blot on human samples (fig 3a). Cancer Res (2017) ncbi
domestic rabbit monoclonal (D20B7)
  • western blot; human; 1:1000; loading ...; fig 8a
Cell Signaling Technology BMI1 antibody (Cell Signaling Technology, 6964s) was used in western blot on human samples at 1:1000 (fig 8a). Sci Rep (2017) ncbi
domestic rabbit monoclonal (D42B3)
  • western blot; human; loading ...; fig 1d
Cell Signaling Technology BMI1 antibody (Cell Signaling Technology, 5856) was used in western blot on human samples (fig 1d). J Biol Chem (2017) ncbi
domestic rabbit monoclonal (D20B7)
  • western blot; human; 1:1000; loading ...; fig 1f
In order to assess the effects of hypoxia on cellular senescence and the immunomodulatory abilities of hUCB-mesenchymal stem cells, Cell Signaling Technology BMI1 antibody (Cell Signaling, 6964s) was used in western blot on human samples at 1:1000 (fig 1f). Aging (Albany NY) (2016) ncbi
domestic rabbit monoclonal (D20B7)
  • western blot; human; 1:1000; loading ...; fig 2c
Cell Signaling Technology BMI1 antibody (Cell signaling, 6964) was used in western blot on human samples at 1:1000 (fig 2c). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2a
Cell Signaling Technology BMI1 antibody (Cell Signaling, 2830) was used in western blot on human samples (fig 2a). Sci Rep (2016) ncbi
domestic rabbit monoclonal (D20B7)
  • immunohistochemistry - paraffin section; human; loading ...; fig 5a
  • immunoprecipitation; human; loading ...; fig 3d
  • western blot; human; loading ...; fig 3d
Cell Signaling Technology BMI1 antibody (Cell Signaling, 6964) was used in immunohistochemistry - paraffin section on human samples (fig 5a), in immunoprecipitation on human samples (fig 3d) and in western blot on human samples (fig 3d). J Mol Med (Berl) (2016) ncbi
domestic rabbit monoclonal (D42B3)
  • western blot; human; fig 6
  • western blot; mouse; fig 5
Cell Signaling Technology BMI1 antibody (Cell Signaling Tech, 5856) was used in western blot on human samples (fig 6) and in western blot on mouse samples (fig 5). Mol Cancer (2015) ncbi
domestic rabbit monoclonal (D42B3)
  • immunohistochemistry - frozen section; mouse; fig 3
  • chromatin immunoprecipitation; mouse; fig 2
  • western blot; mouse; fig 2
Cell Signaling Technology BMI1 antibody (Cell Signaling Technology, 5856) was used in immunohistochemistry - frozen section on mouse samples (fig 3), in chromatin immunoprecipitation on mouse samples (fig 2) and in western blot on mouse samples (fig 2). Aging Cell (2015) ncbi
domestic rabbit monoclonal (D20B7)
  • western blot; human; 1:1000; fig 4
In order to characterize BMI1 expression and proliferative capacity of high-grade gliomas regulated by nuclear-encoded cytochrome c oxcidse subunit 4, Cell Signaling Technology BMI1 antibody (Cell Signaling, 6964) was used in western blot on human samples at 1:1000 (fig 4). Oncotarget (2015) ncbi
domestic rabbit monoclonal (D20B7)
  • western blot; human
Cell Signaling Technology BMI1 antibody (Cell signaling, D20B7) was used in western blot on human samples . Biochem Biophys Res Commun (2015) ncbi
domestic rabbit monoclonal (D20B7)
  • chromatin immunoprecipitation; rat; fig 3
Cell Signaling Technology BMI1 antibody (Cell Signaling, 6964S) was used in chromatin immunoprecipitation on rat samples (fig 3). Nat Neurosci (2015) ncbi
domestic rabbit monoclonal (D20B7)
  • chromatin immunoprecipitation; human; 1:100
  • immunoprecipitation; human; 1:1000
  • western blot; human; 1:1000
In order to study the Involvement of EED in the organization of polycomb group complexes, Cell Signaling Technology BMI1 antibody (Cell Signaling, 6964) was used in chromatin immunoprecipitation on human samples at 1:100, in immunoprecipitation on human samples at 1:1000 and in western blot on human samples at 1:1000. Nat Commun (2014) ncbi
BD Biosciences
mouse monoclonal (P51-311)
  • flow cytometry; human
In order to correlate the expression of CD200 in various types of cancer with the responses to chemotherapy and radiation, BD Biosciences BMI1 antibody (BD Bioscience, 562528) was used in flow cytometry on human samples . Head Neck (2015) ncbi
Articles Reviewed
  1. Bakhoum M, Francis J, Agustinus A, Earlie E, Di Bona M, Abramson D, et al. Loss of polycomb repressive complex 1 activity and chromosomal instability drive uveal melanoma progression. Nat Commun. 2021;12:5402 pubmed publisher
  2. Chen M, Zhou J, Wang P, Ye Y, Liu Y, Zhou J, et al. BMI1 activates P-glycoprotein via transcription repression of miR-3682-3p and enhances chemoresistance of bladder cancer cell. Aging (Albany NY). 2021;13:18310-18330 pubmed publisher
  3. Maroni G, Bassal M, Krishnan I, Fhu C, Savova V, Zilionis R, et al. Identification of a targetable KRAS-mutant epithelial population in non-small cell lung cancer. Commun Biol. 2021;4:370 pubmed publisher
  4. Shields C, Potlapalli S, Cuya Smith S, Chappell S, Chen D, Martinez D, et al. Epigenetic regulator BMI1 promotes alveolar rhabdomyosarcoma proliferation and constitutes a novel therapeutic target. Mol Oncol. 2021;: pubmed publisher
  5. Li W, Zhang N, Jin C, Long M, Rajabi H, Yasumizu Y, et al. MUC1-C drives stemness in progression of colitis to colorectal cancer. JCI Insight. 2020;5: pubmed publisher
  6. Yasuda S, Tsuchiya H, Kaiho A, Guo Q, Ikeuchi K, Endo A, et al. Stress- and ubiquitylation-dependent phase separation of the proteasome. Nature. 2020;578:296-300 pubmed publisher
  7. Zhan Y, Li R, Feng C, Li X, Huang S, Wang L, et al. Chlorogenic acid inhibits esophageal squamous cell carcinoma growth in vitro and in vivo by downregulating the expression of BMI1 and SOX2. Biomed Pharmacother. 2020;121:109602 pubmed publisher
  8. Wang Z, Feng X, Molinolo A, Martin D, Vitale Cross L, Nohata N, et al. 4E-BP1 Is a Tumor Suppressor Protein Reactivated by mTOR Inhibition in Head and Neck Cancer. Cancer Res. 2019;: pubmed publisher
  9. Chen X, Hu L, Yang H, Ma H, Ye K, Zhao C, et al. DHHC protein family targets different subsets of glioma stem cells in specific niches. J Exp Clin Cancer Res. 2019;38:25 pubmed publisher
  10. Jin L, Vu T, Yuan G, Datta P. STRAP Promotes Stemness of Human Colorectal Cancer via Epigenetic Regulation of the NOTCH Pathway. Cancer Res. 2017;77:5464-5478 pubmed publisher
  11. Patel N, Garikapati K, Pandita R, Singh D, Pandita T, Bhadra U, et al. miR-15a/miR-16 down-regulates BMI1, impacting Ub-H2A mediated DNA repair and breast cancer cell sensitivity to doxorubicin. Sci Rep. 2017;7:4263 pubmed publisher
  12. Wheaton K, Sarkari F, Stanly Johns B, Davarinejad H, Egorova O, Kaustov L, et al. UbE2E1/UBCH6 Is a Critical in Vivo E2 for the PRC1-catalyzed Ubiquitination of H2A at Lys-119. J Biol Chem. 2017;292:2893-2902 pubmed publisher
  13. Lee J, Yu K, Kim H, Kang I, Kim J, Lee B, et al. BMI1 inhibits senescence and enhances the immunomodulatory properties of human mesenchymal stem cells via the direct suppression of MKP-1/DUSP1. Aging (Albany NY). 2016;8:1670-89 pubmed publisher
  14. Mansouri S, Singh S, Alamsahebpour A, Burrell K, Li M, Karabork M, et al. DICER governs characteristics of glioma stem cells and the resulting tumors in xenograft mouse models of glioblastoma. Oncotarget. 2016;7:56431-56446 pubmed publisher
  15. Li H, Mai R, Huang H, Chou C, Chang Y, Chang Y, et al. DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma. Sci Rep. 2016;6:28637 pubmed publisher
  16. Choe K, Nicolae C, Constantin D, Imamura Kawasawa Y, Delgado Diaz M, De S, et al. HUWE1 interacts with PCNA to alleviate replication stress. EMBO Rep. 2016;17:874-86 pubmed publisher
  17. Zheng G, Li N, Jia X, Peng C, Luo L, Deng Y, et al. MYCN-mediated miR-21 overexpression enhances chemo-resistance via targeting CADM1 in tongue cancer. J Mol Med (Berl). 2016;94:1129-1141 pubmed
  18. Martínez Fernández M, Dueñas M, Feber A, Segovia C, García Escudero R, Rubio C, et al. A Polycomb-mir200 loop regulates clinical outcome in bladder cancer. Oncotarget. 2015;6:42258-75 pubmed publisher
  19. Lee S, Litan A, Li Z, Graves B, Lindsey S, Barwe S, et al. Na,K-ATPase β1-subunit is a target of sonic hedgehog signaling and enhances medulloblastoma tumorigenicity. Mol Cancer. 2015;14:159 pubmed publisher
  20. Wei X, Dou X, Bai J, Luo X, Qiu S, Xi D, et al. ERα inhibits epithelial-mesenchymal transition by suppressing Bmi1 in breast cancer. Oncotarget. 2015;6:21704-17 pubmed
  21. Yamakoshi K, Katano S, Iida M, Kimura H, Okuma A, Ikemoto Uezumi M, et al. Dysregulation of the Bmi-1/p16(Ink⁴a) pathway provokes an aging-associated decline of submandibular gland function. Aging Cell. 2015;14:616-24 pubmed publisher
  22. Oliva C, Markert T, Gillespie G, Griguer C. Nuclear-encoded cytochrome c oxidase subunit 4 regulates BMI1 expression and determines proliferative capacity of high-grade gliomas. Oncotarget. 2015;6:4330-44 pubmed
  23. Murata K, Sato S, Haruta M, Goshima T, Chiba Y, Takahashi S, et al. Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis. Biochem Biophys Res Commun. 2015;458:470-475 pubmed publisher
  24. Koo J, Mazei Robison M, LaPlant Q, Egervári G, Braunscheidel K, Adank D, et al. Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area. Nat Neurosci. 2015;18:415-22 pubmed publisher
  25. Jung Y, Vermeer P, Vermeer D, Lee S, Goh A, Ahn H, et al. CD200: association with cancer stem cell features and response to chemoradiation in head and neck squamous cell carcinoma. Head Neck. 2015;37:327-35 pubmed publisher
  26. Cao Q, Wang X, Zhao M, Yang R, Malik R, Qiao Y, et al. The central role of EED in the orchestration of polycomb group complexes. Nat Commun. 2014;5:3127 pubmed publisher
  27. Liu H, Zhang W, Jia Y, Yu Q, Grau G, Peng L, et al. Single-cell clones of liver cancer stem cells have the potential of differentiating into different types of tumor cells. Cell Death Dis. 2013;4:e857 pubmed publisher