This is a Validated Antibody Database (VAD) review about human BLM, based on 22 published articles (read how Labome selects the articles), using BLM antibody in all methods. It is aimed to help Labome visitors find the most suited BLM antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
BLM synonym: RECQ2; RECQL2; RECQL3; Bloom syndrome protein; Bloom syndrome, RecQ helicase-like; DNA helicase, RecQ-like type 2; recQ protein-like 3

Abcam
rabbit polyclonal
  • immunocytochemistry; human; 1:500; loading ...; fig 4f
Abcam BLM antibody (Abcam, ab2179) was used in immunocytochemistry on human samples at 1:500 (fig 4f). Science (2018) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4d
Abcam BLM antibody (Abcam, ab2179) was used in western blot on human samples at 1:1000 (fig 4d). Cell Rep (2018) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:500; loading ...; fig 3e
Abcam BLM antibody (Abcam, ab2179) was used in immunocytochemistry on human samples at 1:500 (fig 3e). Science (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 4a
Abcam BLM antibody (Abcam, ab2179) was used in western blot on human samples (fig 4a). Mol Cell (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 2a
Abcam BLM antibody (Abcam, ab2179) was used in western blot on human samples (fig 2a). Genes Dev (2017) ncbi
rabbit polyclonal
  • western blot; human; fig s2
Abcam BLM antibody (abcam, ab476) was used in western blot on human samples (fig s2). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig s6a
Abcam BLM antibody (Abcam, ab2179) was used in western blot on mouse samples (fig s6a). Mol Cell (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig s4
Abcam BLM antibody (Abcam, ab2179) was used in immunocytochemistry on human samples at 1:100 (fig s4). Mol Biol Cell (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:250; loading ...; fig 4c
Abcam BLM antibody (Abcam, ab2179) was used in immunocytochemistry on human samples at 1:250 (fig 4c). DNA Repair (Amst) (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
Abcam BLM antibody (Abcam, ab476) was used in western blot on human samples at 1:1000. DNA Repair (Amst) (2015) ncbi
rabbit polyclonal
  • western blot; human
Abcam BLM antibody (Abcam, ab2179) was used in western blot on human samples . Cell Death Dis (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human
Abcam BLM antibody (Abcam, Ab2179) was used in immunocytochemistry on human samples . J Virol (2013) ncbi
rabbit polyclonal
  • western blot; human
In order to study the promotion of c-Myc degradation by BLM helicase and its effect on tumor initiation, Abcam BLM antibody (Abcam, ab476) was used in western blot on human samples . J Cell Sci (2013) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse
  • western blot; mouse
Abcam BLM antibody (Abcam, Ab2179) was used in immunocytochemistry on mouse samples and in western blot on mouse samples . Science (2013) ncbi
Bethyl
rabbit polyclonal
  • western blot; human; 1:1000; fig 3
Bethyl BLM antibody (Bethyl, A300-110A) was used in western blot on human samples at 1:1000 (fig 3). Methods (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 7
Bethyl BLM antibody (Bethyl laboratories, A300-572A) was used in western blot on mouse samples (fig 7). PLoS ONE (2016) ncbi
rabbit polyclonal
  • RNA immunoprecipitation; human
In order to develop reagents to study RNA binding proteins, Bethyl BLM antibody (Bethyl, A300-110A) was used in RNA immunoprecipitation on human samples . Mol Cell (2016) ncbi
rabbit polyclonal
  • western blot; human
In order to show that that BLM undergoes K48-linked ubiquitylation and subsequent degradation during mitosis via Fbw7alpha, Bethyl BLM antibody (Bethyl, A300-110A) was used in western blot on human samples . Oncogene (2016) ncbi
Invitrogen
rabbit polyclonal
  • immunocytochemistry; mouse; 1:100; fig 6
  • immunocytochemistry; human; 1:100; fig 6
In order to analyze telomere markers and alternative lengthening in BRCA1 defective cells, Invitrogen BLM antibody (Thermo Fisher, PA5-27384) was used in immunocytochemistry on mouse samples at 1:100 (fig 6) and in immunocytochemistry on human samples at 1:100 (fig 6). Genes Chromosomes Cancer (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (4i317)
  • western blot; human; 1:500; fig s4
Santa Cruz Biotechnology BLM antibody (Santa Cruz, sc-70426) was used in western blot on human samples at 1:500 (fig s4). Nat Commun (2014) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot; human; loading ...; fig 3s3
In order to investigate DNA repair in CD44+/CD24- cells, Sigma-Aldrich BLM antibody (Atlas Antibodies, HPA005689) was used in western blot on human samples (fig 3s3). elife (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 2
Sigma-Aldrich BLM antibody (Sigma, HPA005689) was used in immunocytochemistry on human samples (fig 2). EMBO J (2015) ncbi
Articles Reviewed
  1. Saldivar J, Hamperl S, Bocek M, Chung M, Bass T, Cisneros Soberanis F, et al. An intrinsic S/G2 checkpoint enforced by ATR. Science. 2018;361:806-810 pubmed publisher
  2. Tay L, Krishnan V, Sankar H, Chong Y, Chuang L, Tan T, et al. RUNX Poly(ADP-Ribosyl)ation and BLM Interaction Facilitate the Fanconi Anemia Pathway of DNA Repair. Cell Rep. 2018;24:1747-1755 pubmed publisher
  3. Somyajit K, Gupta R, Sedlackova H, Neelsen K, Ochs F, Rask M, et al. Redox-sensitive alteration of replisome architecture safeguards genome integrity. Science. 2017;358:797-802 pubmed publisher
  4. Biehs R, Steinlage M, Barton O, Juhasz S, Künzel J, Spies J, et al. DNA Double-Strand Break Resection Occurs during Non-homologous End Joining in G1 but Is Distinct from Resection during Homologous Recombination. Mol Cell. 2017;65:671-684.e5 pubmed publisher
  5. Gong Y, Handa N, Kowalczykowski S, de Lange T. PHF11 promotes DSB resection, ATR signaling, and HR. Genes Dev. 2017;31:46-58 pubmed publisher
  6. Pal D, Pertot A, Shirole N, Yao Z, Anaparthy N, Garvin T, et al. TGF-β reduces DNA ds-break repair mechanisms to heighten genetic diversity and adaptability of CD44+/CD24- cancer cells. elife. 2017;6: pubmed publisher
  7. Hampp S, Kiessling T, Buechle K, Mansilla S, Thomale J, Rall M, et al. DNA damage tolerance pathway involving DNA polymerase ? and the tumor suppressor p53 regulates DNA replication fork progression. Proc Natl Acad Sci U S A. 2016;113:E4311-9 pubmed publisher
  8. Kargaran P, Yasaei H, Anjomani Virmouni S, Mangiapane G, Slijepcevic P. Analysis of alternative lengthening of telomere markers in BRCA1 defective cells. Genes Chromosomes Cancer. 2016;55:864-76 pubmed publisher
  9. Lu X, Parvathaneni S, Li X, Lal A, Sharma S. Transcriptome guided identification of novel functions of RECQ1 helicase. Methods. 2016;108:111-7 pubmed publisher
  10. Braun J, Meixner A, Brachner A, Foisner R. The GIY-YIG Type Endonuclease Ankyrin Repeat and LEM Domain-Containing Protein 1 (ANKLE1) Is Dispensable for Mouse Hematopoiesis. PLoS ONE. 2016;11:e0152278 pubmed publisher
  11. Sundararaman B, Zhan L, Blue S, Stanton R, Elkins K, Olson S, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016;61:903-13 pubmed publisher
  12. Kibe T, Zimmermann M, de Lange T. TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres. Mol Cell. 2016;61:236-46 pubmed publisher
  13. Kharat S, Tripathi V, Damodaran A, Priyadarshini R, Chandra S, Tikoo S, et al. Mitotic phosphorylation of Bloom helicase at Thr182 is required for its proteasomal degradation and maintenance of chromosomal stability. Oncogene. 2016;35:1025-38 pubmed publisher
  14. Mackay D, Ullman K. ATR and a Chk1-Aurora B pathway coordinate postmitotic genome surveillance with cytokinetic abscission. Mol Biol Cell. 2015;26:2217-26 pubmed publisher
  15. Meena J, Cerutti A, Beichler C, Morita Y, Bruhn C, Kumar M, et al. Telomerase abrogates aneuploidy-induced telomere replication stress, senescence and cell depletion. EMBO J. 2015;34:1371-84 pubmed publisher
  16. Kumari A, Owen N, Juarez E, McCullough A. BLM protein mitigates formaldehyde-induced genomic instability. DNA Repair (Amst). 2015;28:73-82 pubmed publisher
  17. Bursomanno S, Beli P, Khan A, Minocherhomji S, Wagner S, Bekker Jensen S, et al. Proteome-wide analysis of SUMO2 targets in response to pathological DNA replication stress in human cells. DNA Repair (Amst). 2015;25:84-96 pubmed publisher
  18. Chan Y, West S. Spatial control of the GEN1 Holliday junction resolvase ensures genome stability. Nat Commun. 2014;5:4844 pubmed publisher
  19. Lu H, Fang E, Sykora P, Kulikowicz T, Zhang Y, Becker K, et al. Senescence induced by RECQL4 dysfunction contributes to Rothmund-Thomson syndrome features in mice. Cell Death Dis. 2014;5:e1226 pubmed publisher
  20. Hu L, Filippakis H, Huang H, Yen T, Gjoerup O. Replication stress and mitotic dysfunction in cells expressing simian virus 40 large T antigen. J Virol. 2013;87:13179-92 pubmed publisher
  21. Chandra S, Priyadarshini R, Madhavan V, Tikoo S, Hussain M, Mudgal R, et al. Enhancement of c-Myc degradation by BLM helicase leads to delayed tumor initiation. J Cell Sci. 2013;126:3782-95 pubmed publisher
  22. Zimmermann M, Lottersberger F, Buonomo S, Sfeir A, de Lange T. 53BP1 regulates DSB repair using Rif1 to control 5' end resection. Science. 2013;339:700-4 pubmed publisher