This is a Validated Antibody Database (VAD) review about human BEST1, based on 11 published articles (read how Labome selects the articles), using BEST1 antibody in all methods. It is aimed to help Labome visitors find the most suited BEST1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
BEST1 synonym: ARB; BEST; BMD; Best1V1Delta2; RP50; TU15B; VMD2

Abcam
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:200; loading ...; fig 1d
Abcam BEST1 antibody (Abcam, ab14929) was used in immunocytochemistry on human samples at 1:200 (fig 1d). Int J Ophthalmol (2021) ncbi
mouse monoclonal (E6-6)
  • immunocytochemistry; human; fig 3c
In order to characterize the differentiation of RPE cells, Abcam BEST1 antibody (Abcam, ab2182) was used in immunocytochemistry on human samples (fig 3c). Stem Cell Res Ther (2017) ncbi
mouse monoclonal (E6-6)
  • western blot; human; 1:5000; loading ...; fig 6b
In order to identify conditions in which low-passage ARPE-19 cells express genes specific to native human RPE cells, Abcam BEST1 antibody (Abcam, ab2182) was used in western blot on human samples at 1:5000 (fig 6b). Mol Vis (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:100; fig 5A
In order to establish an application to generate retinal pigmented epithelium from induced pluripotent stem cells, Abcam BEST1 antibody (Abcam, ab14927) was used in immunohistochemistry on human samples at 1:100 (fig 5A). PLoS ONE (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:400; fig 4
In order to characterize retinal pigment epithelium cells derived from three-dimensional human embryonic stem cell cultures, Abcam BEST1 antibody (Abcam, ab14929) was used in immunocytochemistry on human samples at 1:400 (fig 4). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:200; tbl 1
  • immunohistochemistry - paraffin section; human; 1:200; tbl 1
Abcam BEST1 antibody (Abcam, ab14927) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (tbl 1) and in immunohistochemistry - paraffin section on human samples at 1:200 (tbl 1). Sci Rep (2016) ncbi
mouse monoclonal (E6-6)
  • immunocytochemistry; human; 1:500
  • western blot; human; 1:2500
In order to describe the reprogramming of adult human dermal fibroblasts to iPSCs and their differentiation to retinal pigment epithelium cells, Abcam BEST1 antibody (Abcam, ab2182) was used in immunocytochemistry on human samples at 1:500 and in western blot on human samples at 1:2500. Neuromolecular Med (2014) ncbi
mouse monoclonal (E6-6)
  • immunohistochemistry - paraffin section; human; 1:200
  • immunocytochemistry; human; 1:200
Abcam BEST1 antibody (Abcam, ab2182) was used in immunohistochemistry - paraffin section on human samples at 1:200 and in immunocytochemistry on human samples at 1:200. PLoS ONE (2014) ncbi
Novus Biologicals
mouse monoclonal (E6-6)
  • immunocytochemistry; human; 1:100; fig 3f
Novus Biologicals BEST1 antibody (Novus, NB300-164) was used in immunocytochemistry on human samples at 1:100 (fig 3f). Front Cell Dev Biol (2021) ncbi
mouse monoclonal (E6-6)
  • immunohistochemistry; human; 1:200; fig 4
In order to establish an application to generate retinal pigmented epithelium from induced pluripotent stem cells, Novus Biologicals BEST1 antibody (Novus, NB300-164) was used in immunohistochemistry on human samples at 1:200 (fig 4). PLoS ONE (2017) ncbi
mouse monoclonal (E6-6)
  • immunocytochemistry; dogs; 1:500; loading ...; fig 1b
  • western blot; dogs; 1:4000; loading ...; fig 1a
In order to characterize autosomal recessive bestrophinopathy mutations, Novus Biologicals BEST1 antibody (Novus Biologicals, NB300-164) was used in immunocytochemistry on dogs samples at 1:500 (fig 1b) and in western blot on dogs samples at 1:4000 (fig 1a). Dis Model Mech (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E6-6)
  • immunocytochemistry; human; loading ...; fig 1a
Santa Cruz Biotechnology BEST1 antibody (Santa Cruz Biotechnology, sc-32792) was used in immunocytochemistry on human samples (fig 1a). Mol Ther Methods Clin Dev (2021) ncbi
Articles Reviewed
  1. Li J, Qiu C, Wei Y, Yuan W, Liu J, Cui W, et al. Human Amniotic Epithelial Stem Cell-Derived Retinal Pigment Epithelium Cells Repair Retinal Degeneration. Front Cell Dev Biol. 2021;9:737242 pubmed publisher
  2. Xu H, Li Q, Zou T, Yin Z. Development-related mitochondrial properties of retinal pigment epithelium cells derived from hEROs. Int J Ophthalmol. 2021;14:1138-1150 pubmed publisher
  3. Salas A, Duarri A, Fontrodona L, Ram xed rez D, Badia A, Isla Magran xe9 H, et al. Cell therapy with hiPSC-derived RPE cells and RPCs prevents visual function loss in a rat model of retinal degeneration. Mol Ther Methods Clin Dev. 2021;20:688-702 pubmed publisher
  4. Hazim R, Karumbayaram S, Jiang M, Dimashkie A, Lopes V, Li D, et al. Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. Stem Cell Res Ther. 2017;8:217 pubmed publisher
  5. Samuel W, Jaworski C, Postnikova O, Kutty R, Duncan T, Tan L, et al. Appropriately differentiated ARPE-19 cells regain phenotype and gene expression profiles similar to those of native RPE cells. Mol Vis. 2017;23:60-89 pubmed
  6. Geng Z, Walsh P, Truong V, Hill C, Ebeling M, Kapphahn R, et al. Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PLoS ONE. 2017;12:e0173575 pubmed publisher
  7. Uggenti C, Briant K, Streit A, Thomson S, Koay Y, Baines R, et al. Restoration of mutant bestrophin-1 expression, localisation and function in a polarised epithelial cell model. Dis Model Mech. 2016;9:1317-1328 pubmed
  8. Wu W, Zeng Y, Li Z, Li Q, Xu H, Yin Z. Features specific to retinal pigment epithelium cells derived from three-dimensional human embryonic stem cell cultures - a new donor for cell therapy. Oncotarget. 2016;7:22819-33 pubmed publisher
  9. Brennan S, Wilkinson W, Tseng H, Finney B, Monk B, Dibble H, et al. The extracellular calcium-sensing receptor regulates human fetal lung development via CFTR. Sci Rep. 2016;6:21975 pubmed publisher
  10. Brandl C, Zimmermann S, Milenkovic V, Rosendahl S, Grassmann F, Milenkovic A, et al. In-depth characterisation of Retinal Pigment Epithelium (RPE) cells derived from human induced pluripotent stem cells (hiPSC). Neuromolecular Med. 2014;16:551-64 pubmed publisher
  11. Kanemura H, Go M, Shikamura M, Nishishita N, Sakai N, Kamao H, et al. Tumorigenicity studies of induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium (RPE) for the treatment of age-related macular degeneration. PLoS ONE. 2014;9:e85336 pubmed publisher