This is a Validated Antibody Database (VAD) review about human B2M, based on 22 published articles (read how Labome selects the articles), using B2M antibody in all methods. It is aimed to help Labome visitors find the most suited B2M antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
B2M synonym: IMD43

Knockout validation
Abcam
domestic rabbit monoclonal (EP2978Y)
  • western blot knockout validation; mouse; loading ...; fig 4b
Abcam B2M antibody (Abcam, EP2978Y) was used in western blot knockout validation on mouse samples (fig 4b). JCI Insight (2018) ncbi
Abcam
domestic rabbit monoclonal (EP2978Y)
  • western blot knockout validation; mouse; 1:500; fig 7
In order to identify major histocompatibility complex class I as a regulator of asymmetry, Abcam B2M antibody (Abcam, ab75853) was used in western blot knockout validation on mouse samples at 1:500 (fig 7). J Physiol (2013) ncbi
BioLegend
mouse monoclonal (2M2)
  • flow cytometry; human; loading ...; fig 2b
BioLegend B2M antibody (Biolegend, 316306) was used in flow cytometry on human samples (fig 2b). Proc Natl Acad Sci U S A (2021) ncbi
mouse monoclonal (2M2)
  • flow cytometry; human; loading ...; fig s8d
BioLegend B2M antibody (Biolegend, 316312) was used in flow cytometry on human samples (fig s8d). Nat Biotechnol (2020) ncbi
mouse monoclonal (2M2)
  • immunohistochemistry; human; loading ...
BioLegend B2M antibody (BioLegened, 316308) was used in immunohistochemistry on human samples . elife (2019) ncbi
mouse monoclonal (2M2)
  • flow cytometry; human; fig 4a
BioLegend B2M antibody (BioLegend, 316312) was used in flow cytometry on human samples (fig 4a). Cell (2018) ncbi
mouse monoclonal (2M2)
  • flow cytometry; human; loading ...; fig 2b
BioLegend B2M antibody (BioLegend, 2M2) was used in flow cytometry on human samples (fig 2b). J Immunol (2017) ncbi
mouse monoclonal (2M2)
  • flow cytometry; human; fig s3a
BioLegend B2M antibody (BioLegend, 2M2) was used in flow cytometry on human samples (fig s3a). Nature (2017) ncbi
Abcam
domestic rabbit monoclonal (EP2978Y)
  • western blot knockout validation; mouse; loading ...; fig 4b
Abcam B2M antibody (Abcam, EP2978Y) was used in western blot knockout validation on mouse samples (fig 4b). JCI Insight (2018) ncbi
domestic rabbit monoclonal (EP2978Y)
  • immunocytochemistry; mouse; loading ...; fig 3g
Abcam B2M antibody (Abcam, ab75853) was used in immunocytochemistry on mouse samples (fig 3g). Science (2018) ncbi
domestic rabbit monoclonal (EP2978Y)
  • western blot; mouse; 1:1000; loading ...; fig s1b
Abcam B2M antibody (abcam, ab75853) was used in western blot on mouse samples at 1:1000 (fig s1b). J Exp Med (2017) ncbi
domestic rabbit monoclonal (EP2978Y)
  • western blot; mouse; 1:5000; fig s6
In order to study the benefits of heterochronic parabiosis in young and old mice, Abcam B2M antibody (Abcam, ab75853) was used in western blot on mouse samples at 1:5000 (fig s6). Nat Commun (2016) ncbi
domestic rabbit monoclonal (EP2978Y)
  • western blot; human; 1:2000; fig 2a
Abcam B2M antibody (Abcam, ab75853) was used in western blot on human samples at 1:2000 (fig 2a). BMC Cancer (2016) ncbi
domestic rabbit monoclonal (EP2978Y)
  • western blot; mouse; 1:2500; fig 2
Abcam B2M antibody (Abcam, ab75853) was used in western blot on mouse samples at 1:2500 (fig 2). Nat Med (2015) ncbi
domestic rabbit monoclonal (EP2978Y)
  • immunocytochemistry; mouse; fig s6
  • immunohistochemistry; mouse; fig 7
Abcam B2M antibody (abcam, ab75853) was used in immunocytochemistry on mouse samples (fig s6) and in immunohistochemistry on mouse samples (fig 7). Oncotarget (2015) ncbi
mouse monoclonal (B2M-01)
  • western blot; human; 1:1000; loading ...; fig 2b
Abcam B2M antibody (Abcam, ab759) was used in western blot on human samples at 1:1000 (fig 2b). BMC Neurosci (2015) ncbi
domestic rabbit monoclonal (EP2978Y)
  • western blot knockout validation; mouse; 1:500; fig 7
In order to identify major histocompatibility complex class I as a regulator of asymmetry, Abcam B2M antibody (Abcam, ab75853) was used in western blot knockout validation on mouse samples at 1:500 (fig 7). J Physiol (2013) ncbi
mouse monoclonal (B2M-01)
  • blocking or activating experiments; human; 25 ug/mL
Abcam B2M antibody (Abcam, ab21899) was used in blocking or activating experiments on human samples at 25 ug/mL. Methods (2013) ncbi
Invitrogen
mouse monoclonal (A4)
  • immunohistochemistry; human; 1:200; loading ...; fig 1c
Invitrogen B2M antibody (E-Bioscience, 14-9958) was used in immunohistochemistry on human samples at 1:200 (fig 1c). JCO Precis Oncol (2019) ncbi
mouse monoclonal (B2M-01)
  • flow cytometry; human; loading ...; tbl 1
In order to investigate somatic reprogramming of adult cells to induced pluripotent stem cells, Invitrogen B2M antibody (Thermo Fisher, A15737) was used in flow cytometry on human samples (tbl 1). J Vis Exp (2016) ncbi
mouse monoclonal (B2M-01)
  • flow cytometry; domestic horse; fig 1
In order to study MHC class I-restricted, virus-specific CTL responses in horses, Invitrogen B2M antibody (Caltag, B2M-01) was used in flow cytometry on domestic horse samples (fig 1). J Immunol (2003) ncbi
Santa Cruz Biotechnology
mouse monoclonal (S19.8)
  • flow cytometry; mouse; loading ...
Santa Cruz Biotechnology B2M antibody (Santa Cruz, SC-32241) was used in flow cytometry on mouse samples . Science (2016) ncbi
mouse monoclonal (BBM.1)
  • immunohistochemistry - paraffin section; human; 1:100; fig 1
  • western blot; human; fig s4
In order to study T-cell mediated degeneration of catacholaminergic neurons expressing MHC I, Santa Cruz Biotechnology B2M antibody (Santa Cruz, sc-13565) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1) and in western blot on human samples (fig s4). Nat Commun (2014) ncbi
Abnova
mouse monoclonal (3F9-2C2)
  • immunohistochemistry - paraffin section; human; 1:100; fig 1
In order to study T-cell mediated degeneration of catacholaminergic neurons expressing MHC I, Abnova B2M antibody (Abnova, H00000567-M01) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1). Nat Commun (2014) ncbi
BD Biosciences
mouse monoclonal (TÜ99)
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences B2M antibody (BD, 551337) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
Articles Reviewed
  1. Zhang Y, Chen Y, Li Y, Huang F, Luo B, Yuan Y, et al. The ORF8 protein of SARS-CoV-2 mediates immune evasion through down-regulating MHC-Ι. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  2. DeWeirdt P, Sanson K, Sangree A, Hegde M, Hanna R, Feeley M, et al. Optimization of AsCas12a for combinatorial genetic screens in human cells. Nat Biotechnol. 2020;: pubmed publisher
  3. Middha S, Yaeger R, Shia J, Stadler Z, King S, Guercio S, et al. Majority of B2M-Mutant and -Deficient Colorectal Carcinomas Achieve Clinical Benefit From Immune Checkpoint Inhibitor Therapy and Are Microsatellite Instability-High. JCO Precis Oncol. 2019;3: pubmed publisher
  4. Tischer D, WEINER O. Light-based tuning of ligand half-life supports kinetic proofreading model of T cell signaling. elife. 2019;8: pubmed publisher
  5. Sato Y, Bolzenius J, Eteleeb A, Su X, Maher C, Sehn J, et al. CD4+ T cells induce rejection of urothelial tumors after immune checkpoint blockade. JCI Insight. 2018;3: pubmed publisher
  6. Theisen D, Davidson J, Briseño C, Gargaro M, Lauron E, Wang Q, et al. WDFY4 is required for cross-presentation in response to viral and tumor antigens. Science. 2018;362:694-699 pubmed publisher
  7. Chung H, Calis J, Wu X, Sun T, Yu Y, Sarbanes S, et al. Human ADAR1 Prevents Endogenous RNA from Triggering Translational Shutdown. Cell. 2018;172:811-824.e14 pubmed publisher
  8. Khrimian L, Obri A, Ramos Brossier M, Rousseaud A, Moriceau S, Nicot A, et al. Gpr158 mediates osteocalcin's regulation of cognition. J Exp Med. 2017;214:2859-2873 pubmed publisher
  9. Zhang X, Lian X, Dai Z, Zheng H, Chen X, Zheng Y. ?3-Deletion Isoform of HLA-A11 Modulates Cytotoxicity of NK Cells: Correlations with HIV-1 Infection of Cells. J Immunol. 2017;199:2030-2042 pubmed publisher
  10. Patel S, Sanjana N, Kishton R, Eidizadeh A, Vodnala S, Cam M, et al. Identification of essential genes for cancer immunotherapy. Nature. 2017;548:537-542 pubmed publisher
  11. Rebo J, Mehdipour M, Gathwala R, Causey K, Liu Y, Conboy M, et al. A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood. Nat Commun. 2016;7:13363 pubmed publisher
  12. Barau J, Teissandier A, Zamudio N, Roy S, Nalesso V, Herault Y, et al. The DNA methyltransferase DNMT3C protects male germ cells from transposon activity. Science. 2016;354:909-912 pubmed
  13. Padhan N, Nordling T, Sundstrom M, Akerud P, Birgisson H, Nygren P, et al. High sensitivity isoelectric focusing to establish a signaling biomarker for the diagnosis of human colorectal cancer. BMC Cancer. 2016;16:683 pubmed publisher
  14. Quintanilla R, Asprer J, Sylakowski K, Lakshmipathy U. Kinetic Measurement and Real Time Visualization of Somatic Reprogramming. J Vis Exp. 2016;: pubmed publisher
  15. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  16. Smith L, He Y, Park J, Bieri G, Snethlage C, Lin K, et al. β2-microglobulin is a systemic pro-aging factor that impairs cognitive function and neurogenesis. Nat Med. 2015;21:932-7 pubmed publisher
  17. Yousef H, Conboy M, Morgenthaler A, Schlesinger C, Bugaj L, Paliwal P, et al. Systemic attenuation of the TGF-β pathway by a single drug simultaneously rejuvenates hippocampal neurogenesis and myogenesis in the same old mammal. Oncotarget. 2015;6:11959-78 pubmed
  18. Raha Chowdhury R, Raha A, Forostyak S, Zhao J, Stott S, Bomford A. Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci. 2015;16:24 pubmed publisher
  19. Cebrián C, Zucca F, Mauri P, Steinbeck J, Studer L, Scherzer C, et al. MHC-I expression renders catecholaminergic neurons susceptible to T-cell-mediated degeneration. Nat Commun. 2014;5:3633 pubmed publisher
  20. Kawahara A, Kurauchi S, Fukata Y, Martínez Hernández J, Yagihashi T, Itadani Y, et al. Neuronal major histocompatibility complex class I molecules are implicated in the generation of asymmetries in hippocampal circuitry. J Physiol. 2013;591:4777-91 pubmed publisher
  21. Zhao H, Gorshkova I, Fu G, Schuck P. A comparison of binding surfaces for SPR biosensing using an antibody-antigen system and affinity distribution analysis. Methods. 2013;59:328-35 pubmed publisher
  22. McGuire T, Leib S, Mealey R, Fraser D, Prieur D. Presentation and binding affinity of equine infectious anemia virus CTL envelope and matrix protein epitopes by an expressed equine classical MHC class I molecule. J Immunol. 2003;171:1984-93 pubmed