This is a Validated Antibody Database (VAD) review about human B-Raf, based on 65 published articles (read how Labome selects the articles), using B-Raf antibody in all methods. It is aimed to help Labome visitors find the most suited B-Raf antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
B-Raf synonym: B-RAF1; B-raf; BRAF1; NS7; RAFB1; serine/threonine-protein kinase B-raf; 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; murine sarcoma viral (v-raf) oncogene homolog B1; proto-oncogene B-Raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1
Knockout validation
Santa Cruz Biotechnology mouse monoclonal (F-7) |
| In order to study prevention of allergic skin disease by epidermal RAF, Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, sc-5284) was used in western blot on human samples at 1:1000 (fig 9) and in western blot knockout validation on mouse samples at 1:1000 (fig 3). elife (2016) ncbi |
Santa Cruz Biotechnology mouse monoclonal (F-7) |
| Santa Cruz Biotechnology B-Raf antibody (Santa, sc-5284) was used in western blot knockout validation on mouse samples at 1:500 (fig 4b). Cell Signal (2013) ncbi |
Santa Cruz Biotechnology
mouse monoclonal (F-3) |
| Santa Cruz Biotechnology B-Raf antibody (Santa, sc-55522) was used in western blot on human samples at 1:1000 (fig ex1m). Nature (2018) ncbi |
mouse monoclonal (F-7) |
| Santa Cruz Biotechnology B-Raf antibody (SantaCruz, sc-5284) was used in western blot on human samples at 1:1000 (fig 5c). Nat Commun (2017) ncbi |
mouse monoclonal (F-7) |
| In order to show that PHB1 is highly expressed in non-small cell lung cancers patients and correlates with poor survival, Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, sc-5284) was used in western blot on human samples (fig 3e). Oncogene (2017) ncbi |
mouse monoclonal (F-7) |
| In order to characterize the molecular identity of uterine carcinosarcomas., Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, sc-5284) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi |
mouse monoclonal (F-7) |
| In order to describe the features of 228 primary cervical cancers, Santa Cruz Biotechnology B-Raf antibody (SantaCruz, sc-5284) was used in reverse phase protein lysate microarray on human samples (fig 3a). Nature (2017) ncbi |
mouse monoclonal (F-7) |
| Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, sc-5284) was used in western blot on human samples (fig 2c). Oncogene (2017) ncbi |
mouse monoclonal (F-7) |
| In order to clarify the interaction of SPRED1 with Ras and Raf, Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, sc-5284) was used in proximity ligation assay on hamsters samples (fig 3d). Mol Cell Biol (2016) ncbi |
mouse monoclonal (F-7) |
| In order to study prevention of allergic skin disease by epidermal RAF, Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, sc-5284) was used in western blot on human samples at 1:1000 (fig 9) and in western blot knockout validation on mouse samples at 1:1000 (fig 3). elife (2016) ncbi |
mouse monoclonal (F-7) |
| In order to develop a patient-derived xenograft platform and use it to identify genes that contribute the cancer resistance of melanoma patients treated with BRAF inhibitors, Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, F7) was used in western blot on human samples (fig 6b). Cell Rep (2016) ncbi |
mouse monoclonal |
| In order to develop a patient-derived xenograft platform and use it to identify genes that contribute the cancer resistance of melanoma patients treated with BRAF inhibitors, Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, F7) was used in western blot on human samples (fig 6b). Cell Rep (2016) ncbi |
mouse monoclonal (F-7) |
| Santa Cruz Biotechnology B-Raf antibody (santa Cruz, sc-5284) was used in western blot on human samples at 1:1000 (fig s1). Sci Rep (2016) ncbi |
mouse monoclonal (F-7) |
| In order to determine limits on FAK-dependent invasion and enhancement of the response to melanoma treatment with BRAF inhibitors due to HSP70 inhibition, Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, sc-5284) was used in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 2). Cancer Res (2016) ncbi |
mouse monoclonal (F-7) |
| Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, sc-5284) was used in western blot on human samples at 1:1000 (tbl 2). elife (2016) ncbi |
mouse monoclonal (F-7) |
| Santa Cruz Biotechnology B-Raf antibody (santa cruz, sc-5284) was used in western blot on human samples (fig 1). Carcinogenesis (2015) ncbi |
mouse monoclonal (F-7) |
| Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, sc-5284) was used in western blot on human samples (fig S3A). Autophagy (2015) ncbi |
mouse monoclonal (F-7) |
| Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, sc-5284) was used in western blot on human samples (fig 3b). Oncotarget (2015) ncbi |
mouse monoclonal (F-7) |
| Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, sc-5284) was used in immunoprecipitation on human samples at 1:1000 (fig 2) and in immunocytochemistry on human samples at 1:25 (fig 5). Oncogene (2016) ncbi |
mouse monoclonal (F-3) |
| In order to investigate how BRAF/MAPK activity regulates intestinal stem cell populations and contributes to colon cancer, Santa Cruz Biotechnology B-Raf antibody (Santa Cruz, 55522) was used in western blot on mouse samples at 1:200 (fig 2). Oncogene (2015) ncbi |
mouse monoclonal (F-7) |
| Santa Cruz Biotechnology B-Raf antibody (Santa Cruz Biotechnology, sc-5284) was used in western blot on human samples . Cancer Res (2014) ncbi |
mouse monoclonal (F-7) |
| Santa Cruz Biotechnology B-Raf antibody (Santa, sc-5284) was used in western blot knockout validation on mouse samples at 1:500 (fig 4b). Cell Signal (2013) ncbi |
mouse monoclonal (F-7) |
| Santa Cruz Biotechnology B-Raf antibody (Santa Cruz Biotechnology, sc-5284) was used in western blot on hamsters samples . Mol Cell Biol (2013) ncbi |
Abcam
rabbit monoclonal (EPR2207) |
| In order to identify specific genes involved in leukemogenesis through comprehensive ex vivo transposon mutagenesis, Abcam B-Raf antibody (Abcam, EPR2207) was used in western blot on mouse samples at 1:1000 (fig 2c). Cancer Res (2016) ncbi |
rabbit monoclonal (RM8) |
| Abcam B-Raf antibody (Abcam, Ab200535) was used in western blot on Human herpesvirus 1 samples (fig 2). J Cell Biol (2015) ncbi |
MilliporeSigma
rabbit polyclonal |
| MilliporeSigma B-Raf antibody (Sigma, HPA001328) was used in western blot on human samples (fig s3c). Cancer Res (2018) ncbi |
mouse monoclonal (3C6) |
| MilliporeSigma B-Raf antibody (Sigma, WH0000673M1) was used in immunoprecipitation on human samples (tbl 3). elife (2016) ncbi |
rabbit polyclonal |
| In order to study Val600 in the activation portion of BRAF and its peculiar yin-yang of kinase activation and unfolding, MilliporeSigma B-Raf antibody (SIGMA, HPA001328) was used in western blot on human samples (fig 3). elife (2016) ncbi |
rabbit polyclonal |
| MilliporeSigma B-Raf antibody (SIGMA, HPA001328) was used in western blot on human samples (fig s1). Sci Rep (2015) ncbi |
Cell Signaling Technology
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, 9427) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi |
rabbit polyclonal |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, 2696) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, 9427) was used in western blot on human samples at 1:1000 (fig 1e). Science (2018) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, cs-9427) was used in western blot on human samples (fig 3b). Int J Oncol (2018) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, 9427S) was used in western blot on mouse samples (fig 4g). Cancer Cell (2018) ncbi |
rabbit monoclonal (56A6) |
| In order to show that PHB1 is highly expressed in non-small cell lung cancers patients and correlates with poor survival, Cell Signaling Technology B-Raf antibody (Cell Signalling, 9427) was used in western blot on human samples (fig 3b). Oncogene (2017) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, 9427) was used in western blot on human samples (fig 3a). J Biol Chem (2017) ncbi |
rabbit monoclonal (56A6) |
| In order to observe that chronic presence of internalized Escherichia coli leads to enhanced oncogenicity in colon cancer cells, Cell Signaling Technology B-Raf antibody (cell signalling, 9427) was used in western blot on human samples (fig 1b). Cell Death Dis (2017) ncbi |
rabbit monoclonal (56A6) |
| In order to characterize the molecular identity of uterine carcinosarcomas., Cell Signaling Technology B-Raf antibody (CST, 9427) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling Technology, 9427) was used in western blot on human samples (fig 4d). Cell (2017) ncbi |
rabbit monoclonal (56A6) |
| In order to describe the features of 228 primary cervical cancers, Cell Signaling Technology B-Raf antibody (Cell Signaling, 9427) was used in reverse phase protein lysate microarray on human samples (fig 3a). Nature (2017) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (cell signalling, 9427) was used in western blot on mouse samples (fig 2a). Proc Natl Acad Sci U S A (2017) ncbi |
rabbit polyclonal |
| In order to assess the effects of LY3009120, a panRAF and RAF dimer inhibitor, in human models of colorectal cancer, Cell Signaling Technology B-Raf antibody (Cell Signaling, 2696) was used in western blot on human samples (fig 1c). Oncotarget (2017) ncbi |
rabbit monoclonal (56A6) |
| In order to assess the effects of LY3009120, a panRAF and RAF dimer inhibitor, in human models of colorectal cancer, Cell Signaling Technology B-Raf antibody (Cell Signaling, 9427) was used in western blot on human samples (fig 1c). Oncotarget (2017) ncbi |
rabbit polyclonal |
| In order to report the changes associated with gonadotropin-releasing hormone agonist treatment, Cell Signaling Technology B-Raf antibody (Cell Signaling, 9421S) was used in western blot on dog samples (tbl 1). Mol Reprod Dev (2016) ncbi |
rabbit monoclonal (56A6) |
| In order to investigate how 6-C-(E-phenylethenyl)naringenin inhibits cancer cell growth, Cell Signaling Technology B-Raf antibody (Cell signaling, 9427) was used in western blot on human samples (fig 5c). Eur J Cancer (2016) ncbi |
rabbit polyclonal |
| In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology B-Raf antibody (Cell Signaling, 2696) was used in western blot on human samples at 1:100 (fig st1). Nat Commun (2016) ncbi |
rabbit polyclonal |
| In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology B-Raf antibody (Cell Signaling, 9421) was used in western blot on human samples at 1:200 (fig st1). Nat Commun (2016) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (CST, 9427) was used in western blot on human samples at 1:1000. Cell Death Dis (2016) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell signaling, 9427) was used in western blot on human samples (fig 3). FASEB J (2016) ncbi |
rabbit polyclonal |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, 9421) was used in western blot on human samples (fig 4). BMC Complement Altern Med (2016) ncbi |
rabbit monoclonal (56A6) |
| In order to research induction of epithelial-to-mesenchymal transition by Polo-like kinase 1 and promotion of epithelial cell motility by activating CRAF/ERK signaling, Cell Signaling Technology B-Raf antibody (Cell signaling, 9427) was used in western blot on human samples at 1:500 (fig 8). elife (2016) ncbi |
rabbit polyclonal |
| Cell Signaling Technology B-Raf antibody (Cell Signaling Tech, 9421S) was used in western blot on human samples at 1:1000 (fig 5). Oncol Lett (2016) ncbi |
rabbit monoclonal (55C6) |
| In order to elucidate a screening that identifies kinases involved in prostate cancer visceral and bone metastasis, Cell Signaling Technology B-Raf antibody (Cell Signaling Technology, 55C6) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 4) and in western blot on human samples at 1:1000. Proc Natl Acad Sci U S A (2016) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, 9427) was used in western blot on human samples at 1:1000 (fig 3a). Am J Physiol Lung Cell Mol Physiol (2016) ncbi |
rabbit polyclonal |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, 2696) was used in western blot on human samples at 1:500 (fig 3c). Am J Physiol Lung Cell Mol Physiol (2016) ncbi |
rabbit polyclonal |
| Cell Signaling Technology B-Raf antibody (Cell Signaling Technology, 9421) was used in western blot on human samples (fig 5) and in western blot on mouse samples (fig 5). Oncogene (2016) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling Technology, 9427) was used in western blot on mouse samples (fig 5) and in western blot on human samples (fig 5). Oncogene (2016) ncbi |
rabbit polyclonal |
| In order to study the molecular signaling responsible for retinoic acid-induced differentiation, Cell Signaling Technology B-Raf antibody (Cell Signaling, 9421) was used in western blot on human samples . PLoS ONE (2015) ncbi |
rabbit polyclonal |
| In order to study the molecular signaling responsible for retinoic acid-induced differentiation, Cell Signaling Technology B-Raf antibody (Cell Signaling, 9431) was used in western blot on human samples . PLoS ONE (2015) ncbi |
rabbit polyclonal |
| In order to identify host signaling dynamics upon Burkholderia spp. infection by a reverse-phase protein microarray-based screen, Cell Signaling Technology B-Raf antibody (Cell Signaling, 9421) was used in other on mouse samples at 1:500 (fig s1). Front Microbiol (2015) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, 9427S) was used in western blot on hamsters samples (fig 2f). elife (2015) ncbi |
rabbit polyclonal |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, 2696) was used in western blot on human samples (fig 3b). Oncotarget (2015) ncbi |
rabbit monoclonal (55C6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling Technology, 9433) was used in western blot on human samples (fig 2e). Oncogene (2016) ncbi |
rabbit polyclonal |
| Cell Signaling Technology B-Raf antibody (CST, 9421) was used in western blot on human samples . Cell Signal (2015) ncbi |
rabbit monoclonal (55C6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling Technology, 9433) was used in western blot on human samples at 1:1000 (fig 5a). Cancer Cell Int (2015) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling Technology, 9427) was used in western blot on mouse samples (fig 4). Mol Biol Cell (2015) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling Technology, 9427) was used in western blot on human samples (fig S3). PLoS ONE (2015) ncbi |
rabbit monoclonal (55C6) |
| In order to test if NBDHEX and MC3181 have antitumor activity against melanoma cells resistant to vemurafenib, Cell Signaling Technology B-Raf antibody (Cell Signaling Technology, 9433) was used in western blot on human samples at 1:1000. Biochem Pharmacol (2015) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling Technology, 9427) was used in western blot on human samples . Int J Oncol (2015) ncbi |
rabbit polyclonal |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, # 2696) was used in western blot on human samples . Oncotarget (2015) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling technology, 9427) was used in western blot on hamsters samples . Med Microbiol Immunol (2014) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signalling, 9427) was used in western blot on human samples . Cell Microbiol (2014) ncbi |
rabbit monoclonal (56A6) |
| In order to study the ERbB signaling network with a view to understanding the basis for context-specific signaling plasticity, Cell Signaling Technology B-Raf antibody (Cell Signaling, 9427) was used in western blot on human samples . Sci Signal (2013) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, #9427P) was used in western blot on human samples at 1:1000. J Mol Histol (2014) ncbi |
rabbit polyclonal |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, 9421) was used in western blot on human samples . J Biol Chem (2013) ncbi |
rabbit polyclonal |
| In order to study the GNAS locus as a driver in 20q amplified breast cancer found in an siRNA screen, Cell Signaling Technology B-Raf antibody (Cell Signalling Technology, 9421) was used in western blot on human samples (fig 6). Oncogene (2014) ncbi |
rabbit monoclonal (56A6) |
| Cell Signaling Technology B-Raf antibody (Cell Signaling, 9427) was used in western blot on human samples . Cancer Cell Int (2013) ncbi |
BD Biosciences
mouse monoclonal (13/B-RAF) |
| BD Biosciences B-Raf antibody (BD, 612375) was used in immunoprecipitation on mouse samples (fig 4s1b, 4s1d). elife (2016) ncbi |
mouse monoclonal (13/B-RAF) |
| In order to demonstrate the bipartite role of Hsp90 in chaperoning CRAF kinase, BD Biosciences B-Raf antibody (BD Biosciences, 612375) was used in western blot on human samples at 1:5000 (fig 1a). J Biol Chem (2016) ncbi |
EMD Millipore
rabbit polyclonal |
| In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, EMD Millipore B-Raf antibody (Upstate, 07-583) was used in western blot on human samples at 1:200 (fig st1). Nat Commun (2016) ncbi |
Articles Reviewed
- Mitra S, Ghosh B, Gayen N, Roy J, Mandal A. Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation. J Biol Chem. 2016;291:24579-24593 pubmed
- Subramaniam S, Ozdener M, Abdoul Azize S, Saito K, Malik B, Maquart G, et al. ERK1/2 activation in human taste bud cells regulates fatty acid signaling and gustatory perception of fat in mice and humans. FASEB J. 2016;30:3489-3500 pubmed
- Zhang Z, Zhang H, Peng T, Li D, Xu J. Melittin suppresses cathepsin S-induced invasion and angiogenesis via blocking of the VEGF-A/VEGFR-2/MEK1/ERK1/2 pathway in human hepatocellular carcinoma. Oncol Lett. 2016;11:610-618 pubmed
- Condelli V, Maddalena F, Sisinni L, Lettini G, Matassa D, Piscazzi A, et al. Targeting TRAP1 as a downstream effector of BRAF cytoprotective pathway: a novel strategy for human BRAF-driven colorectal carcinoma. Oncotarget. 2015;6:22298-309 pubmed
- Petti C, Picco G, Martelli M, Trisolini E, Bucci E, Perera T, et al. Truncated RAF kinases drive resistance to MET inhibition in MET-addicted cancer cells. Oncotarget. 2015;6:221-33 pubmed
articles
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