This is a Validated Antibody Database (VAD) review about human Apaf-1, based on 16 published articles (read how Labome selects the articles), using Apaf-1 antibody in all methods. It is aimed to help Labome visitors find the most suited Apaf-1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Apaf-1 synonym: APAF-1; CED4; apoptotic protease-activating factor 1

Enzo Life Sciences
rat monoclonal (2E12)
  • western blot; human; loading ...; fig 4f
Enzo Life Sciences Apaf-1 antibody (Enzo Life Sciences, ALX-804-348-C100) was used in western blot on human samples (fig 4f). Cell Rep (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (5E11)
  • immunohistochemistry - paraffin section; rat; 1:100; loading ...; fig 5
In order to test if Malaysian jungle Tualang honey alters the hematological parameters, estrogen, estrogen receptors, and pro- and anti-apoptotic protein expression in induced breast cancer in rats, Santa Cruz Biotechnology Apaf-1 antibody (Santa Cruz, SC-65891) was used in immunohistochemistry - paraffin section on rat samples at 1:100 (fig 5). BMC Complement Altern Med (2017) ncbi
mouse monoclonal (2E10)
  • western blot; rat
  • western blot; human
  • western blot; mouse
Santa Cruz Biotechnology Apaf-1 antibody (Santa Cruz Biotechnology, sc-65890) was used in western blot on rat samples , in western blot on human samples and in western blot on mouse samples . J Neurosci (2014) ncbi
Abcam
rabbit polyclonal
  • western blot; rat; fig 3
In order to analyze acute pulmonary exposure to mountaintop removal mining particulate matter and cardiac and mitochondrial dysfunction, Abcam Apaf-1 antibody (Abcam, ab2000) was used in western blot on rat samples (fig 3). Am J Physiol Heart Circ Physiol (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; rat
In order to study adriamycin in non-cancerous tissues, Abcam Apaf-1 antibody (Abcam, Ab2001) was used in immunocytochemistry on rat samples . Biol Trace Elem Res (2016) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; fig 8
In order to research how porphyromonas gingivalis mediates the cell death profile in Ox-LDL and TNF-alpha pre-treated endothelial cells, Invitrogen Apaf-1 antibody (ThermoFischer, PA5-19894) was used in western blot on human samples (fig 8). PLoS ONE (2016) ncbi
MilliporeSigma
rabbit polyclonal
  • immunohistochemistry; human; 1:500; loading ...; fig 5c
MilliporeSigma Apaf-1 antibody (Sigma-Aldrich Co, HPA031373) was used in immunohistochemistry on human samples at 1:500 (fig 5c). Nat Commun (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (D7G4)
  • western blot; mouse; 1:500; loading ...; fig s6a
Cell Signaling Technology Apaf-1 antibody (Cell Signaling, 8723) was used in western blot on mouse samples at 1:500 (fig s6a). Nat Cell Biol (2017) ncbi
rabbit monoclonal (D7G4)
  • western blot; human; fig S1
Cell Signaling Technology Apaf-1 antibody (Cell Signaling, 8723) was used in western blot on human samples (fig S1). Nat Commun (2016) ncbi
rabbit monoclonal (D7G4)
  • western blot; human; 1:1000
Cell Signaling Technology Apaf-1 antibody (Cell Signaling, 8723) was used in western blot on human samples at 1:1000. J Cell Biochem (2016) ncbi
rabbit monoclonal (D7G4)
  • western blot; mouse
Cell Signaling Technology Apaf-1 antibody (Cell Signaling Technology, 8723) was used in western blot on mouse samples . Mol Cell Biol (2014) ncbi
rabbit monoclonal (D5C3)
  • western blot; mouse; 1:1000
Cell Signaling Technology Apaf-1 antibody (Cell Signaling Technology, 8969) was used in western blot on mouse samples at 1:1000. J Biol Chem (2014) ncbi
BD Biosciences
rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 3h
In order to elucidate how FADD contributes to mitochondrial-associated apoptosis, BD Biosciences Apaf-1 antibody (Cell Signaling, 559,683) was used in western blot on human samples at 1:500 (fig 3h). Mol Cell Biochem (2016) ncbi
mouse monoclonal (24/Apaf-1)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences Apaf-1 antibody (BD, 24) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (24/Apaf-1)
  • western blot; human
BD Biosciences Apaf-1 antibody (BD Transduction Laboratories, 24) was used in western blot on human samples . Biochem Biophys Res Commun (2014) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human
In order to investigate the role of Apaf1 in axonogenesis, EMD Millipore Apaf-1 antibody (Millipore, AB16941) was used in western blot on human samples . Cell Mol Life Sci (2015) ncbi
Articles Reviewed
  1. Leslie P, Franklin D, Liu Y, Zhang Y. p53 Regulates the Expression of LRP1 and Apoptosis through a Stress Intensity-Dependent MicroRNA Feedback Loop. Cell Rep. 2018;24:1484-1495 pubmed publisher
  2. Giampazolias E, Zunino B, Dhayade S, Bock F, Cloix C, Cao K, et al. Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency. Nat Cell Biol. 2017;19:1116-1129 pubmed publisher
  3. Ahmed S, Othman N. The anti-cancer effects of Tualang honey in modulating breast carcinogenesis: an experimental animal study. BMC Complement Altern Med. 2017;17:208 pubmed publisher
  4. Ranjan K, Pathak C. Expression of FADD and cFLIPL balances mitochondrial integrity and redox signaling to substantiate apoptotic cell death. Mol Cell Biochem. 2016;422:135-150 pubmed
  5. Bugueno I, Khelif Y, Seelam N, Morand D, Tenenbaum H, Davideau J, et al. Porphyromonas gingivalis Differentially Modulates Cell Death Profile in Ox-LDL and TNF-α Pre-Treated Endothelial Cells. PLoS ONE. 2016;11:e0154590 pubmed publisher
  6. Au Yeung C, Co N, Tsuruga T, Yeung T, Kwan S, Leung C, et al. Exosomal transfer of stroma-derived miR21 confers paclitaxel resistance in ovarian cancer cells through targeting APAF1. Nat Commun. 2016;7:11150 pubmed publisher
  7. Lopez J, Bessou M, Riley J, Giampazolias E, Todt F, Rochegüe T, et al. Mito-priming as a method to engineer Bcl-2 addiction. Nat Commun. 2016;7:10538 pubmed publisher
  8. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  9. Nichols C, Shepherd D, Knuckles T, Thapa D, Stricker J, Stapleton P, et al. Cardiac and mitochondrial dysfunction following acute pulmonary exposure to mountaintop removal mining particulate matter. Am J Physiol Heart Circ Physiol. 2015;309:H2017-30 pubmed publisher
  10. Boratyn E, Nowak I, Horwacik I, Durbas M, Mistarz A, Kukla M, et al. Monocyte Chemoattractant Protein-Induced Protein 1 Overexpression Modulates Transcriptome, Including MicroRNA, in Human Neuroblastoma Cells. J Cell Biochem. 2016;117:694-707 pubmed publisher
  11. Yapislar H, Taşkın E, Ozdas S, Akin D, Sonmez E. Counteraction of Apoptotic and Inflammatory Effects of Adriamycin in the Liver Cell Culture by Clinopitolite. Biol Trace Elem Res. 2016;170:373-81 pubmed publisher
  12. De Zio D, Molinari F, Rizza S, Gatta L, Ciotti M, Salvatore A, et al. Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth. Cell Mol Life Sci. 2015;72:4173-91 pubmed publisher
  13. Sabirzhanov B, Zhao Z, Stoica B, Loane D, Wu J, Borroto C, et al. Downregulation of miR-23a and miR-27a following experimental traumatic brain injury induces neuronal cell death through activation of proapoptotic Bcl-2 proteins. J Neurosci. 2014;34:10055-71 pubmed publisher
  14. Morita A, Ariyasu S, Wang B, Asanuma T, Onoda T, Sawa A, et al. AS-2, a novel inhibitor of p53-dependent apoptosis, prevents apoptotic mitochondrial dysfunction in a transcription-independent manner and protects mice from a lethal dose of ionizing radiation. Biochem Biophys Res Commun. 2014;450:1498-504 pubmed publisher
  15. Vreeland A, Yu S, Levi L, de Barros Rossetto D, Noy N. Transcript stabilization by the RNA-binding protein HuR is regulated by cellular retinoic acid-binding protein 2. Mol Cell Biol. 2014;34:2135-46 pubmed publisher
  16. Tsuneki M, Madri J. CD44 regulation of endothelial cell proliferation and apoptosis via modulation of CD31 and VE-cadherin expression. J Biol Chem. 2014;289:5357-70 pubmed publisher