This is a Validated Antibody Database (VAD) review about human AXIN1, based on 26 published articles (read how Labome selects the articles), using AXIN1 antibody in all methods. It is aimed to help Labome visitors find the most suited AXIN1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
AXIN1 synonym: AXIN; PPP1R49; axin-1; axis inhibition protein 1; axis inhibitor 1; fused, mouse, homolog of; protein phosphatase 1, regulatory subunit 49

Knockout validation
R&D Systems
goat polyclonal
  • western blot knockout validation; human; 1:1250; fig 3-s1
In order to investigate regulatory mechanisms in canonical WNT signaling, R&D Systems AXIN1 antibody (R and D Systems, AF3287) was used in western blot knockout validation on human samples at 1:1250 (fig 3-s1). elife (2016) ncbi
Invitrogen
rabbit polyclonal
  • western blot; human; fig s5
In order to predict Wnt signaling in human colorectal cancers, Invitrogen AXIN1 antibody (Zymed Laboratorie, 34-5900) was used in western blot on human samples (fig s5). PLoS ONE (2012) ncbi
rabbit polyclonal
  • western blot; human; fig 4
In order to elucidate the contributions of LRP5 and LRP6 to Wnt signaling, Invitrogen AXIN1 antibody (Invitrogen, 34-5900) was used in western blot on human samples (fig 4). PLoS ONE (2011) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 1
In order to assess the effects of Wnt-3a and beta-catenin on the differentiation of ReNcell VM human neural progenitor cells, Invitrogen AXIN1 antibody (Zymed, 34-5900) was used in western blot on human samples at 1:1000 (fig 1). Biochem Biophys Res Commun (2010) ncbi
rabbit polyclonal
In order to screen for host factors that modulate human immunodeficiency virus type-1 replication, Invitrogen AXIN1 antibody (Zymed, 34-5900) was used . Biochem Biophys Res Commun (2007) ncbi
rabbit polyclonal
In order to identify and characterize two distinct soluble protein pools containing APC, Invitrogen AXIN1 antibody (Zymed, 34-5900) was used . J Cell Sci (2005) ncbi
R&D Systems
goat polyclonal
  • western blot; human; 1:3000; loading ...; fig 5d
R&D Systems AXIN1 antibody (R&D, AF3287) was used in western blot on human samples at 1:3000 (fig 5d). Mol Cell (2018) ncbi
goat polyclonal
  • western blot knockout validation; human; 1:1250; fig 3-s1
In order to investigate regulatory mechanisms in canonical WNT signaling, R&D Systems AXIN1 antibody (R and D Systems, AF3287) was used in western blot knockout validation on human samples at 1:1250 (fig 3-s1). elife (2016) ncbi
goat polyclonal
  • western blot; human; loading ...; fig 2b
In order to show that Axin point mutants derail Wnt signaling and promote tumor growth in vivo, R&D Systems AXIN1 antibody (R&D Systems, AF3287) was used in western blot on human samples (fig 2b). Nat Struct Mol Biol (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (C76H11)
  • western blot; mouse; 81 ng/ml; loading ...; fig 4c
Cell Signaling Technology AXIN1 antibody (Cell Signaling, C76H11) was used in western blot on mouse samples at 81 ng/ml (fig 4c). Science (2019) ncbi
rabbit monoclonal (C76H11)
  • western blot; human; 1:1000; loading ...; fig 4a
Cell Signaling Technology AXIN1 antibody (Cell Signaling, C76H11) was used in western blot on human samples at 1:1000 (fig 4a). elife (2019) ncbi
rabbit monoclonal (C95H11)
  • western blot; mouse; loading ...; fig 5e
Cell Signaling Technology AXIN1 antibody (Cell Signaling, 2074S) was used in western blot on mouse samples (fig 5e). Cell (2019) ncbi
rabbit monoclonal (C76H11)
  • western blot; human; 1:1000; loading ...; fig 4e, 5a, 5b
Cell Signaling Technology AXIN1 antibody (Cell Signaling, 2087S) was used in western blot on human samples at 1:1000 (fig 4e, 5a, 5b). Mol Cell (2018) ncbi
rabbit monoclonal (C95H11)
  • western blot; human; loading ...; fig 1a
Cell Signaling Technology AXIN1 antibody (Cell Signaling, 2074) was used in western blot on human samples (fig 1a). Oncogene (2018) ncbi
rabbit monoclonal (C95H11)
  • western blot; human; 1:1000; loading ...; fig 2a
Cell Signaling Technology AXIN1 antibody (Cell Signaling, 2074) was used in western blot on human samples at 1:1000 (fig 2a). Nat Commun (2017) ncbi
rabbit monoclonal (C76H11)
  • immunoprecipitation; mouse; fig 5f
  • western blot; mouse; 1:1000; fig 5f
Cell Signaling Technology AXIN1 antibody (Cell Signaling, 2087) was used in immunoprecipitation on mouse samples (fig 5f) and in western blot on mouse samples at 1:1000 (fig 5f). Dev Cell (2017) ncbi
rabbit monoclonal (C95H11)
  • western blot; mouse; loading ...; fig 5a
Cell Signaling Technology AXIN1 antibody (Cell Signaling, 2074) was used in western blot on mouse samples (fig 5a). Bone (2018) ncbi
rabbit monoclonal (C76H11)
  • western blot; mouse; loading ...; fig 3g
Cell Signaling Technology AXIN1 antibody (Cell Signaling, 2087) was used in western blot on mouse samples (fig 3g). J Clin Invest (2017) ncbi
rabbit monoclonal (C76H11)
  • western blot; mouse; loading ...; fig 1a
Cell Signaling Technology AXIN1 antibody (Cell Signaling, 2087) was used in western blot on mouse samples (fig 1a). J Clin Invest (2017) ncbi
rabbit monoclonal (C76H11)
  • western blot; mouse; 1:1000; loading ...; fig 4c
Cell Signaling Technology AXIN1 antibody (Cell signaling, 2087) was used in western blot on mouse samples at 1:1000 (fig 4c). Int J Mol Med (2017) ncbi
rabbit monoclonal (C7B12)
  • western blot; human; 1:1000; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology AXIN1 antibody (Cell Signaling, 3323) was used in western blot on human samples at 1:1000 (fig st1). Nat Commun (2016) ncbi
rabbit monoclonal (C76H11)
  • western blot; mouse; fig 2
In order to determine maintenance of mouse embryonic stem cell identity by a myc-driven self-reinforcing regulatory network, Cell Signaling Technology AXIN1 antibody (Cell Signalling, 2087) was used in western blot on mouse samples (fig 2). Nat Commun (2016) ncbi
rabbit monoclonal (C95H11)
  • western blot; human; 1:1000; fig 6
Cell Signaling Technology AXIN1 antibody (Cell Signaling, 2074) was used in western blot on human samples at 1:1000 (fig 6). Nat Commun (2016) ncbi
rabbit monoclonal (C7B12)
  • western blot; human; loading ...; fig 6
Cell Signaling Technology AXIN1 antibody (Cell Signaling, C7B12) was used in western blot on human samples (fig 6). Mol Cell Proteomics (2015) ncbi
rabbit monoclonal (C76H11)
  • immunoprecipitation; human; fig 5
  • western blot; human; fig 5
Cell Signaling Technology AXIN1 antibody (Cell Signaling Technology, C76H11) was used in immunoprecipitation on human samples (fig 5) and in western blot on human samples (fig 5). Cell (2014) ncbi
rabbit monoclonal (C76H11)
  • western blot; human
Cell Signaling Technology AXIN1 antibody (Cell Signaling Technology, 2C76H11) was used in western blot on human samples . Cell Mol Life Sci (2014) ncbi
EMD Millipore
rabbit polyclonal
  • immunohistochemistry; human; 1:200; loading ...; fig 3d
EMD Millipore AXIN1 antibody (Millipore, 06-1049) was used in immunohistochemistry on human samples at 1:200 (fig 3d). Development (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:200; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, EMD Millipore AXIN1 antibody (Millipore, 17-10140) was used in western blot on human samples at 1:200 (fig st1). Nat Commun (2016) ncbi
mouse monoclonal (A5)
  • immunocytochemistry; human; 1:100
  • western blot; human; 1:500
In order to investigate the role of Zbed3 in malignant phenotype of lung cancer, EMD Millipore AXIN1 antibody (Millipore, 05-1579) was used in immunocytochemistry on human samples at 1:100 and in western blot on human samples at 1:500. Mol Carcinog (2015) ncbi
Articles Reviewed
  1. Choi J, Zhong X, McAlpine W, Liao T, Zhang D, Fang B, et al. LMBR1L regulates lymphopoiesis through Wnt/β-catenin signaling. Science. 2019;364: pubmed publisher
  2. Vallardi G, Allan L, Crozier L, Saurin A. Division of labour between PP2A-B56 isoforms at the centromere and kinetochore. elife. 2019;8: pubmed publisher
  3. Moon S, Huang C, Houlihan S, Regunath K, Freed Pastor W, Morris J, et al. p53 Represses the Mevalonate Pathway to Mediate Tumor Suppression. Cell. 2019;176:564-580.e19 pubmed publisher
  4. Ji L, Lu B, Wang Z, Yang Z, Reece Hoyes J, Russ C, et al. Identification of ICAT as an APC Inhibitor, Revealing Wnt-Dependent Inhibition of APC-Axin Interaction. Mol Cell. 2018;72:37-47.e4 pubmed publisher
  5. Jiang J, Tang S, Xia J, Wen J, Chen S, Shu X, et al. C9orf140, a novel Axin1-interacting protein, mediates the negative feedback loop of Wnt/β-catenin signaling. Oncogene. 2018;37:2992-3005 pubmed publisher
  6. Bhardwaj A, Yang Y, Ueberheide B, Smith S. Whole proteome analysis of human tankyrase knockout cells reveals targets of tankyrase-mediated degradation. Nat Commun. 2017;8:2214 pubmed publisher
  7. Chen X, Wang R, Liu X, Wu Y, Zhou T, Yang Y, et al. A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3? and GSK3? in Regulating Embryonic Stem Cell Fate. Dev Cell. 2017;43:563-576.e4 pubmed publisher
  8. Fujita S, Mukai T, Mito T, Kodama S, Nagasu A, Kittaka M, et al. Pharmacological inhibition of tankyrase induces bone loss in mice by increasing osteoclastogenesis. Bone. 2018;106:156-166 pubmed publisher
  9. Matsumoto Y, La Rose J, Lim M, Adissu H, Law N, Mao X, et al. Ubiquitin ligase RNF146 coordinates bone dynamics and energy metabolism. J Clin Invest. 2017;127:2612-2625 pubmed publisher
  10. Matsumoto Y, LaRose J, Kent O, Lim M, Changoor A, Zhang L, et al. RANKL coordinates multiple osteoclastogenic pathways by regulating expression of ubiquitin ligase RNF146. J Clin Invest. 2017;127:1303-1315 pubmed publisher
  11. Li C, Chang L, Chen Z, Liu Z, Wang Y, Ye Q. The role of lncRNA MALAT1 in the regulation of hepatocyte proliferation during liver regeneration. Int J Mol Med. 2017;39:347-356 pubmed publisher
  12. Lebensohn A, Dubey R, Neitzel L, Tacchelly Benites O, Yang E, Marceau C, et al. Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling. elife. 2016;5: pubmed publisher
  13. Zimmerlin L, Park T, Huo J, Verma K, Pather S, Talbot C, et al. Tankyrase inhibition promotes a stable human naïve pluripotent state with improved functionality. Development. 2016;143:4368-4380 pubmed
  14. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  15. Fagnocchi L, Cherubini A, Hatsuda H, Fasciani A, Mazzoleni S, Poli V, et al. A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity. Nat Commun. 2016;7:11903 pubmed publisher
  16. Yang E, Tacchelly Benites O, Wang Z, Randall M, Tian A, Benchabane H, et al. Wnt pathway activation by ADP-ribosylation. Nat Commun. 2016;7:11430 pubmed publisher
  17. Anvarian Z, Nojima H, Van Kappel E, Madl T, Spit M, Viertler M, et al. Axin cancer mutants form nanoaggregates to rewire the Wnt signaling network. Nat Struct Mol Biol. 2016;23:324-32 pubmed publisher
  18. Traenkle B, Emele F, Anton R, Poetz O, Haeussler R, Maier J, et al. Monitoring interactions and dynamics of endogenous beta-catenin with intracellular nanobodies in living cells. Mol Cell Proteomics. 2015;14:707-23 pubmed publisher
  19. Fan C, Jiang G, Zhang X, Miao Y, Lin X, Luan L, et al. Zbed3 contributes to malignant phenotype of lung cancer via regulating β-catenin and P120-catenin 1. Mol Carcinog. 2015;54 Suppl 1:E138-47 pubmed publisher
  20. Azzolin L, Panciera T, Soligo S, Enzo E, Bicciato S, Dupont S, et al. YAP/TAZ incorporation in the ?-catenin destruction complex orchestrates the Wnt response. Cell. 2014;158:157-70 pubmed publisher
  21. Knoblich K, Wang H, Sharma C, Fletcher A, Turley S, Hemler M. Tetraspanin TSPAN12 regulates tumor growth and metastasis and inhibits ?-catenin degradation. Cell Mol Life Sci. 2014;71:1305-14 pubmed publisher
  22. Tan C, Gardiner B, Hirokawa Y, Layton M, Smith D, Burgess A. Wnt signalling pathway parameters for mammalian cells. PLoS ONE. 2012;7:e31882 pubmed publisher
  23. MacDonald B, Semenov M, Huang H, He X. Dissecting molecular differences between Wnt coreceptors LRP5 and LRP6. PLoS ONE. 2011;6:e23537 pubmed publisher
  24. Hübner R, Schmöle A, Liedmann A, Frech M, Rolfs A, Luo J. Differentiation of human neural progenitor cells regulated by Wnt-3a. Biochem Biophys Res Commun. 2010;400:358-62 pubmed publisher
  25. Kameoka M, Kitagawa Y, Utachee P, Jinnopat P, Dhepakson P, Isarangkura Na Ayuthaya P, et al. Identification of the suppressive factors for human immunodeficiency virus type-1 replication using the siRNA mini-library directed against host cellular genes. Biochem Biophys Res Commun. 2007;359:729-34 pubmed
  26. Penman G, Leung L, Nathke I. The adenomatous polyposis coli protein (APC) exists in two distinct soluble complexes with different functions. J Cell Sci. 2005;118:4741-50 pubmed