This is a Validated Antibody Database (VAD) review about human ATRX, based on 13 published articles (read how Labome selects the articles), using ATRX antibody in all methods. It is aimed to help Labome visitors find the most suited ATRX antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ATRX synonym: JMS; MRX52; RAD54; RAD54L; XH2; XNP; ZNF-HX

Knockout validation
MilliporeSigma
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; human; 1:500; loading ...; fig 2a
MilliporeSigma ATRX antibody (Atlas Antibodies, HPA001906) was used in immunohistochemistry knockout validation on human samples at 1:500 (fig 2a). PLoS ONE (2018) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; fig 4
  • immunoprecipitation; human; fig 2
MilliporeSigma ATRX antibody (Sigma, HPA001906) was used in western blot knockout validation on human samples at 1:1000 (fig 4) and in immunoprecipitation on human samples (fig 2). Epigenetics (2016) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 3b
Abcam ATRX antibody (Abcam, ab97508) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 3b). Nat Commun (2020) ncbi
domestic rabbit polyclonal
  • other; human; loading ...; fig 4c
Abcam ATRX antibody (Abcam, ab97508) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:100; fig 6
  • immunocytochemistry; mouse; 1:100; fig 6
In order to analyze telomere markers and alternative lengthening in BRCA1 defective cells, Abcam ATRX antibody (Abcam, Ab97508) was used in immunocytochemistry on human samples at 1:100 (fig 6) and in immunocytochemistry on mouse samples at 1:100 (fig 6). Genes Chromosomes Cancer (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:800; fig 2
Abcam ATRX antibody (Abcam, ab97508) was used in immunohistochemistry - paraffin section on human samples at 1:800 (fig 2). Oncotarget (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (D-5)
  • western blot; human; 1:250; fig 4
Santa Cruz Biotechnology ATRX antibody (Santa Cruz, D5) was used in western blot on human samples at 1:250 (fig 4). J Virol (2016) ncbi
mouse monoclonal (D-5)
  • immunohistochemistry; human; 1:1000; fig 1
Santa Cruz Biotechnology ATRX antibody (santa Cruz, sc-55584) was used in immunohistochemistry on human samples at 1:1000 (fig 1). Oncotarget (2015) ncbi
mouse monoclonal (D-5)
  • chromatin immunoprecipitation; human; fig 2
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 3c
Santa Cruz Biotechnology ATRX antibody (Santa Cruz Biotechnology, sc-55584) was used in chromatin immunoprecipitation on human samples (fig 2), in immunocytochemistry on human samples (fig 1) and in western blot on human samples (fig 3c). Mol Cell Biol (2015) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; human; 1:333; loading ...; fig 1a
MilliporeSigma ATRX antibody (Sigma-Aldrich, HPA001906) was used in western blot on human samples at 1:333 (fig 1a). J Cell Sci (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; human; 1:500; loading ...; fig 2a
MilliporeSigma ATRX antibody (Atlas Antibodies, HPA001906) was used in immunohistochemistry knockout validation on human samples at 1:500 (fig 2a). PLoS ONE (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:400; loading ...; fig 4c
MilliporeSigma ATRX antibody (Sigma, HPA001906) was used in western blot on human samples at 1:400 (fig 4c). Nat Commun (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 2
In order to assess the responses of ALT- or telomerase-positive cell lines to VE-821 treatment, MilliporeSigma ATRX antibody (Sigma, HPA001906) was used in western blot on human samples (fig 2). Front Oncol (2016) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; fig 4
  • immunoprecipitation; human; fig 2
MilliporeSigma ATRX antibody (Sigma, HPA001906) was used in western blot knockout validation on human samples at 1:1000 (fig 4) and in immunoprecipitation on human samples (fig 2). Epigenetics (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; human; 1:500; fig 2
MilliporeSigma ATRX antibody (Sigma-Aldrich, HPA001906) was used in immunohistochemistry on human samples at 1:500 (fig 2). PLoS Genet (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D1N2E)
  • western blot; human; loading ...; fig 6b
Cell Signaling Technology ATRX antibody (Cell Signaling, 14820) was used in western blot on human samples (fig 6b). PLoS ONE (2018) ncbi
Articles Reviewed
  1. Zeineldin M, Federico S, Chen X, Fan Y, Xu B, Stewart E, et al. MYCN amplification and ATRX mutations are incompatible in neuroblastoma. Nat Commun. 2020;11:913 pubmed publisher
  2. Han M, Napier C, Frölich S, Teber E, Wong T, Noble J, et al. Synthetic lethality of cytolytic HSV-1 in cancer cells with ATRX and PML deficiency. J Cell Sci. 2019;132: pubmed publisher
  3. Brosnan Cashman J, Yuan M, Graham M, Rizzo A, Myers K, Davis C, et al. ATRX loss induces multiple hallmarks of the alternative lengthening of telomeres (ALT) phenotype in human glioma cell lines in a cell line-specific manner. PLoS ONE. 2018;13:e0204159 pubmed publisher
  4. Diplas B, He X, Brosnan Cashman J, Liu H, Chen L, Wang Z, et al. The genomic landscape of TERT promoter wildtype-IDH wildtype glioblastoma. Nat Commun. 2018;9:2087 pubmed publisher
  5. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  6. Deeg K, Chung I, Bauer C, Rippe K. Cancer Cells with Alternative Lengthening of Telomeres Do Not Display a General Hypersensitivity to ATR Inhibition. Front Oncol. 2016;6:186 pubmed publisher
  7. Hahn A, Großkopf A, Jungnickl D, Scholz B, Ensser A. Viral FGARAT Homolog ORF75 of Rhesus Monkey Rhadinovirus Effects Proteasomal Degradation of the ND10 Components SP100 and PML. J Virol. 2016;90:8013-28 pubmed publisher
  8. Kargaran P, Yasaei H, Anjomani Virmouni S, Mangiapane G, Slijepcevic P. Analysis of alternative lengthening of telomere markers in BRCA1 defective cells. Genes Chromosomes Cancer. 2016;55:864-76 pubmed publisher
  9. Valle García D, Qadeer Z, McHugh D, Ghiraldini F, Chowdhury A, Hasson D, et al. ATRX binds to atypical chromatin domains at the 3' exons of zinc finger genes to preserve H3K9me3 enrichment. Epigenetics. 2016;11:398-414 pubmed publisher
  10. Cai J, Zhu P, Zhang C, Li Q, Wang Z, Li G, et al. Detection of ATRX and IDH1-R132H immunohistochemistry in the progression of 211 paired gliomas. Oncotarget. 2016;7:16384-95 pubmed publisher
  11. Mäkinen N, Aavikko M, Heikkinen T, Taipale M, Taipale J, Koivisto Korander R, et al. Exome Sequencing of Uterine Leiomyosarcomas Identifies Frequent Mutations in TP53, ATRX, and MED12. PLoS Genet. 2016;12:e1005850 pubmed publisher
  12. Leeper H, Caron A, Decker P, Jenkins R, LaChance D, Giannini C. IDH mutation, 1p19q codeletion and ATRX loss in WHO grade II gliomas. Oncotarget. 2015;6:30295-305 pubmed publisher
  13. Eid R, Demattei M, Episkopou H, Augé Gouillou C, Decottignies A, Grandin N, et al. Genetic Inactivation of ATRX Leads to a Decrease in the Amount of Telomeric Cohesin and Level of Telomere Transcription in Human Glioma Cells. Mol Cell Biol. 2015;35:2818-30 pubmed publisher