This is a Validated Antibody Database (VAD) review about human ATG14, based on 19 published articles (read how Labome selects the articles), using ATG14 antibody in all methods. It is aimed to help Labome visitors find the most suited ATG14 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ATG14 synonym: ATG14L; BARKOR; KIAA0831

Knockout validation
MBL International
monoclonal (4H8)
  • western blot knockout validation; human; 1:500; loading ...; fig s6c
MBL International ATG14 antibody (MBL International, M184-3) was used in western blot knockout validation on human samples at 1:500 (fig s6c). Proc Natl Acad Sci U S A (2021) ncbi
Abcam
domestic rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 3b
  • western blot; human; loading ...; fig 3a
Abcam ATG14 antibody (Abcam, Ab139727) was used in immunoprecipitation on human samples (fig 3b) and in western blot on human samples (fig 3a). Am J Physiol Cell Physiol (2019) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 3a
Cell Signaling Technology ATG14 antibody (Cell Signaling, 5504) was used in western blot on human samples at 1:1000 (fig 3a). EMBO Mol Med (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 9a
Cell Signaling Technology ATG14 antibody (Cell Signaling Technologies, 5504) was used in western blot on human samples (fig 9a). J Virol (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3g
Cell Signaling Technology ATG14 antibody (Cell Signaling Technology, 5504) was used in western blot on human samples (fig 3g). Autophagy (2019) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:3000; loading ...; fig 4a
Cell Signaling Technology ATG14 antibody (Cell Signaling Technology, 5504S) was used in western blot on mouse samples at 1:3000 (fig 4a). J Lipid Res (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 7d
Cell Signaling Technology ATG14 antibody (CST, 5504) was used in western blot on human samples (fig 7d). elife (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4b
In order to investigate the contribution of PIK3R4 mutations to ciliopathies, Cell Signaling Technology ATG14 antibody (Cell signaling, 5504) was used in western blot on human samples at 1:1000 (fig 4b). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig s4b
In order to propose that ABHD5 has a PNPLA2-independent function in regulating autophagy and tumorigenesis, Cell Signaling Technology ATG14 antibody (Cell Signaling, 5504) was used in western blot on human samples (fig s4b). Autophagy (2016) ncbi
MBL International
monoclonal (4H8)
  • western blot knockout validation; human; 1:500; loading ...; fig s6c
MBL International ATG14 antibody (MBL International, M184-3) was used in western blot knockout validation on human samples at 1:500 (fig s6c). Proc Natl Acad Sci U S A (2021) ncbi
monoclonal (4H8)
  • immunocytochemistry; human; 1:1000; loading ...
  • western blot; human; 1:500; loading ...; fig 2a, 2d
MBL International ATG14 antibody (MBL International Corporation, M184-3) was used in immunocytochemistry on human samples at 1:1000 and in western blot on human samples at 1:500 (fig 2a, 2d). Nature (2021) ncbi
monoclonal (4H8)
  • immunocytochemistry; human; loading ...; fig 3c
MBL International ATG14 antibody (MBL, M184-3) was used in immunocytochemistry on human samples (fig 3c). Dev Cell (2018) ncbi
  • western blot; human; loading ...; fig 5a
MBL International ATG14 antibody (MBL International, PD026) was used in western blot on human samples (fig 5a). PLoS ONE (2017) ncbi
  • immunoprecipitation; mouse; fig 3
MBL International ATG14 antibody (MBL, PD026) was used in immunoprecipitation on mouse samples (fig 3). Nat Commun (2015) ncbi
  • western blot; mouse; fig 3a
  • western blot; human; fig 3a
MBL International ATG14 antibody (MBL International Corporation., PD026) was used in western blot on mouse samples (fig 3a) and in western blot on human samples (fig 3a). EMBO J (2015) ncbi
  • immunocytochemistry; mouse; 1:100; fig s7
In order to use of chaperone-mediated autophagy to facilitate lipolysis by degradation of lipid droplet-associated proteins, MBL International ATG14 antibody (MBL, PD026) was used in immunocytochemistry on mouse samples at 1:100 (fig s7). Nat Cell Biol (2015) ncbi
  • western blot; mouse; fig 1d
  • immunoprecipitation; human; fig 2b
  • western blot; human; fig 1b
In order to demonstrate that G-protein-coupled receptors modulate the levels of Atg14L through ZBTB16, MBL International ATG14 antibody (MBL, PD026) was used in western blot on mouse samples (fig 1d), in immunoprecipitation on human samples (fig 2b) and in western blot on human samples (fig 1b). elife (2015) ncbi
  • immunoprecipitation; human; 1:1000
In order to identify and characterize the Nrbf2-Beclin 1 interaction, MBL International ATG14 antibody (MBL, PD026) was used in immunoprecipitation on human samples at 1:1000. J Biol Chem (2014) ncbi
  • immunoprecipitation; mouse; 1:100
  • western blot; mouse; 1:1000
In order to identify mechanisms that control of PI3K-III activity, MBL International ATG14 antibody (MBL, Z-PD026) was used in immunoprecipitation on mouse samples at 1:100 and in western blot on mouse samples at 1:1000. Nat Commun (2014) ncbi
  • immunoprecipitation; mouse
  • immunocytochemistry; mouse; 1:200
  • western blot; mouse; 1:1000
In order to study the regulation of autophagosome biogenesis by connexins, MBL International ATG14 antibody (MBL, PD026) was used in immunoprecipitation on mouse samples , in immunocytochemistry on mouse samples at 1:200 and in western blot on mouse samples at 1:1000. Nat Cell Biol (2014) ncbi
Articles Reviewed
  1. Wijshake T, Zou Z, Chen B, Zhong L, Xiao G, Xie Y, et al. Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  2. Dong X, Yang Y, Zou Z, Zhao Y, Ci B, Zhong L, et al. Sorting nexin 5 mediates virus-induced autophagy and immunity. Nature. 2021;589:456-461 pubmed publisher
  3. Chen G, Xie W, Nah J, Sauvat A, Liu P, Pietrocola F, et al. 3,4-Dimethoxychalcone induces autophagy through activation of the transcription factors TFE3 and TFEB. EMBO Mol Med. 2019;11:e10469 pubmed publisher
  4. Pringle E, Robinson C, McCormick C. Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication Interferes with mTORC1 Regulation of Autophagy and Viral Protein Synthesis. J Virol. 2019;93: pubmed publisher
  5. Kumar V, Ayasolla K, Jha A, Mishra A, Vashistha H, Lan X, et al. Disrupted APOL1-miR193a Axis Dedifferentiates Podocytes through Autophagy Blockade in APOL1 Risk Milieu. Am J Physiol Cell Physiol. 2019;: pubmed publisher
  6. Ding X, Jiang X, Tian R, Zhao P, Li L, Wang X, et al. RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells. Autophagy. 2019;:1-13 pubmed publisher
  7. Puri C, Vicinanza M, Ashkenazi A, Gratian M, Zhang Q, Bento C, et al. The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A. Dev Cell. 2018;45:114-131.e8 pubmed publisher
  8. Sun H, Krauss R, Chang J, Teng B. PCSK9 deficiency reduces atherosclerosis, apolipoprotein B secretion, and endothelial dysfunction. J Lipid Res. 2018;59:207-223 pubmed publisher
  9. Merrill N, Schipper J, Karnes J, Kauffman A, Martin K, Mackeigan J. PI3K-C2? knockdown decreases autophagy and maturation of endocytic vesicles. PLoS ONE. 2017;12:e0184909 pubmed publisher
  10. Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch A, Muller S, et al. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. elife. 2017;6: pubmed publisher
  11. Stoetzel C, Bär S, De Craene J, Scheidecker S, Etard C, Chicher J, et al. A mutation in VPS15 (PIK3R4) causes a ciliopathy and affects IFT20 release from the cis-Golgi. Nat Commun. 2016;7:13586 pubmed publisher
  12. Peng Y, Miao H, Wu S, Yang W, Zhang Y, Xie G, et al. ABHD5 interacts with BECN1 to regulate autophagy and tumorigenesis of colon cancer independent of PNPLA2. Autophagy. 2016;12:2167-2182 pubmed
  13. Nemazanyy I, Montagnac G, Russell R, Morzyglod L, Burnol A, Guan K, et al. Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling. Nat Commun. 2015;6:8283 pubmed publisher
  14. Munson M, Allen G, Toth R, Campbell D, Lucocq J, Ganley I. mTOR activates the VPS34-UVRAG complex to regulate autolysosomal tubulation and cell survival. EMBO J. 2015;34:2272-90 pubmed publisher
  15. Kaushik S, Cuervo A. Degradation of lipid droplet-associated proteins by chaperone-mediated autophagy facilitates lipolysis. Nat Cell Biol. 2015;17:759-70 pubmed publisher
  16. Zhang T, Dong K, Liang W, Xu D, Xia H, Geng J, et al. G-protein-coupled receptors regulate autophagy by ZBTB16-mediated ubiquitination and proteasomal degradation of Atg14L. elife. 2015;4:e06734 pubmed publisher
  17. Zhong Y, Morris D, Jin L, Patel M, Karunakaran S, Fu Y, et al. Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing Beclin 1-Vps34 complex architecture and reducing intracellular phosphatidylinositol-3 phosphate levels. J Biol Chem. 2014;289:26021-37 pubmed publisher
  18. Lu J, He L, Behrends C, Araki M, Araki K, Jun Wang Q, et al. NRBF2 regulates autophagy and prevents liver injury by modulating Atg14L-linked phosphatidylinositol-3 kinase III activity. Nat Commun. 2014;5:3920 pubmed publisher
  19. Bejarano E, Yuste A, Patel B, Stout R, Spray D, Cuervo A. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 2014;16:401-14 pubmed publisher