This is a Validated Antibody Database (VAD) review about human ATG12, based on 30 published articles (read how Labome selects the articles), using ATG12 antibody in all methods. It is aimed to help Labome visitors find the most suited ATG12 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ATG12 synonym: APG12; APG12L; FBR93; HAPG12

Santa Cruz Biotechnology
mouse monoclonal (C-6)
  • western blot; rat; 1:100
Santa Cruz Biotechnology ATG12 antibody (Santa Cruz Biotechnology, SC271688) was used in western blot on rat samples at 1:100. J Pineal Res (2015) ncbi
mouse monoclonal (C-6)
  • western blot; human; 1:1000; loading ...; fig 6
Santa Cruz Biotechnology ATG12 antibody (Santa Cruz, sc-271688) was used in western blot on human samples at 1:1000 (fig 6). Cell Death Dis (2014) ncbi
GeneTex
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2a
GeneTex ATG12 antibody (Genetex, GTX124181) was used in western blot on human samples (fig 2a). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4a
In order to elucidate the upstream mechanisms of apoptosis triggered by an anti-microtubule drug, ABT-751, GeneTex ATG12 antibody (GeneTex, GTX124181) was used in western blot on human samples at 1:1000 (fig 4a). Toxicol Appl Pharmacol (2016) ncbi
Abcam
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:400; loading ...; fig 2b
Abcam ATG12 antibody (Abcam, ab155589) was used in immunocytochemistry on mouse samples at 1:400 (fig 2b). J Biol Chem (2018) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 1a
In order to target microRNAs involved in autophagy in cystic fibrosis macrophages, MilliporeSigma ATG12 antibody (Sigma-Aldrich, A8731) was used in western blot on mouse samples (fig 1a). Autophagy (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D88H11)
  • western blot; rat; loading ...; fig 2f
Cell Signaling Technology ATG12 antibody (Cell Signaling Technology, 4180) was used in western blot on rat samples (fig 2f). Sci Adv (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 1b
Cell Signaling Technology ATG12 antibody (Cell Signaling, 2010) was used in western blot on human samples (fig 1b). Biochem Biophys Res Commun (2019) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; mouse; 1:1000; loading ...; fig 8c
Cell Signaling Technology ATG12 antibody (Cell Signaling Technologies, 4180) was used in western blot on mouse samples at 1:1000 (fig 8c). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 1s1a
  • western blot; human; loading ...; fig 1s1g
Cell Signaling Technology ATG12 antibody (CST, 2010) was used in immunocytochemistry on human samples (fig 1s1a) and in western blot on human samples (fig 1s1g). elife (2017) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; mouse; loading ...; fig 5a
Cell Signaling Technology ATG12 antibody (CST, 4180) was used in western blot on mouse samples (fig 5a). Proteomics (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; loading ...; fig 5e
Cell Signaling Technology ATG12 antibody (Cell Signaling Technology, 2011S) was used in immunocytochemistry on mouse samples (fig 5e). Cell Death Dis (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 8
In order to study a novel therapeutic strategy for Alzheimer's disease via Pim1 inhibition, Cell Signaling Technology ATG12 antibody (Cell Signaling, 2010) was used in western blot on mouse samples at 1:1000 (fig 8). Mol Neurodegener (2016) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; human; 1:1000; loading ...; fig 1d
Cell Signaling Technology ATG12 antibody (Cell Signaling, 4180) was used in western blot on human samples at 1:1000 (fig 1d). Autophagy (2016) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; mouse; loading ...; fig s8c
Cell Signaling Technology ATG12 antibody (Cell Signaling, 4180) was used in western blot on mouse samples (fig s8c). Nature (2016) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; mouse; fig 5a
In order to research the effects of environmental tobacco smoke on autophagy and longevity, Cell Signaling Technology ATG12 antibody (Cell Signaling, 4180) was used in western blot on mouse samples (fig 5a). Oncotarget (2016) ncbi
domestic rabbit monoclonal (D88H11)
Cell Signaling Technology ATG12 antibody (Cell Signaling, 4180) was used . elife (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:500; fig s1
Cell Signaling Technology ATG12 antibody (Cell Signaling, 2011) was used in western blot on mouse samples at 1:500 (fig s1). J Cell Biol (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:500; fig s1
Cell Signaling Technology ATG12 antibody (Cell Signaling, 2010) was used in western blot on human samples at 1:500 (fig s1). J Cell Biol (2016) ncbi
domestic rabbit monoclonal (D88H11)
  • immunocytochemistry; human; 1:1000; fig 1
In order to investigate impairment of autophagy flux and induction of cell death independent of necroptosis and apoptosis by dual PI-3 kinase/mTOR inhibition, Cell Signaling Technology ATG12 antibody (Cell Signaling, 4180) was used in immunocytochemistry on human samples at 1:1000 (fig 1). Oncotarget (2016) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; human; 1:5000; fig 1
In order to discuss how mutations in superoxide dismutase-1 affect the motor system, Cell Signaling Technology ATG12 antibody (Cell Signaling Technology, 4180) was used in western blot on human samples at 1:5000 (fig 1). Acta Neuropathol Commun (2016) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; mouse; fig 5
Cell Signaling Technology ATG12 antibody (Cell Signaling, 4180) was used in western blot on mouse samples (fig 5). Autophagy (2015) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; human; 1:1000; fig s1
In order to determine how starvation-induced autophagy is promoted by transcriptional regulation of Annexin A2, Cell Signaling Technology ATG12 antibody (Cell Signaling, 4180S) was used in western blot on human samples at 1:1000 (fig s1). Nat Commun (2015) ncbi
domestic rabbit monoclonal (D88H11)
  • immunocytochemistry; human
  • western blot; rat
Cell Signaling Technology ATG12 antibody (Cell Signaling, 4180) was used in immunocytochemistry on human samples and in western blot on rat samples . Toxicol Lett (2015) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; human; fig 3a
Cell Signaling Technology ATG12 antibody (Cell Signaling Technology, 4180) was used in western blot on human samples (fig 3a). Autophagy (2016) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; rat; loading ...; fig 5a
Cell Signaling Technology ATG12 antibody (Cell Signaling, 4180) was used in western blot on rat samples (fig 5a). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; rat; 1:1000
Cell Signaling Technology ATG12 antibody (Cell Signaling Technology, 4180) was used in western blot on rat samples at 1:1000. Neurobiol Dis (2015) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; human; fig 7
  • immunohistochemistry - frozen section; mouse; 1:100; fig 9
In order to test if autophagic vacuoles formation is required for Abeta production by SUMO1, Cell Signaling Technology ATG12 antibody (Cell Signaling Technology, 4180) was used in western blot on human samples (fig 7) and in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 9). Autophagy (2015) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; human; 1:1000
Cell Signaling Technology ATG12 antibody (Cell Signaling, 4180P) was used in western blot on human samples at 1:1000. Biochim Biophys Acta (2015) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; human; 1:1000
Cell Signaling Technology ATG12 antibody (Cell Signaling Technology, 4180) was used in western blot on human samples at 1:1000. Biochim Biophys Acta (2015) ncbi
domestic rabbit monoclonal (D88H11)
  • western blot; human; 1:1000
Cell Signaling Technology ATG12 antibody (Cell Signaling, 4180P) was used in western blot on human samples at 1:1000. Biochim Biophys Acta (2014) ncbi
Articles Reviewed
  1. Xie X, Bi H, Lai S, Zhang Y, Li N, Cao H, et al. The immunoproteasome catalytic β5i subunit regulates cardiac hypertrophy by targeting the autophagy protein ATG5 for degradation. Sci Adv. 2019;5:eaau0495 pubmed publisher
  2. Nitta A, Hori K, Tanida I, Igarashi A, Deyama Y, Ueno T, et al. Blocking LC3 lipidation and ATG12 conjugation reactions by ATG7 mutant protein containing C572S. Biochem Biophys Res Commun. 2019;508:521-526 pubmed publisher
  3. Bansal M, Moharir S, Sailasree S, Sirohi K, Sudhakar C, Sarathi D, et al. Optineurin promotes autophagosome formation by recruiting the autophagy-related Atg12-5-16L1 complex to phagophores containing the Wipi2 protein. J Biol Chem. 2018;293:132-147 pubmed publisher
  4. Liu C, Choi H, Johnson Z, Tian J, Shapiro I, Risbud M. Lack of evidence for involvement of TonEBP and hyperosmotic stimulus in induction of autophagy in the nucleus pulposus. Sci Rep. 2017;7:4543 pubmed publisher
  5. Jung J, Nayak A, Schaeffer V, Starzetz T, Kirsch A, Muller S, et al. Multiplex image-based autophagy RNAi screening identifies SMCR8 as ULK1 kinase activity and gene expression regulator. elife. 2017;6: pubmed publisher
  6. Tsai C, Li C, Cheng Y, Lee C, Liao P, Lin C, et al. The inhibition of lung cancer cell migration by AhR-regulated autophagy. Sci Rep. 2017;7:41927 pubmed publisher
  7. Zhong L, Zhou J, Chen X, Liu J, Liu Z, Chen Y, et al. Quantitative proteomics reveals EVA1A-related proteins involved in neuronal differentiation. Proteomics. 2017;17: pubmed publisher
  8. Wei R, Lin S, Wu W, Chen L, Li C, Chen H, et al. A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells. Toxicol Appl Pharmacol. 2016;311:88-98 pubmed publisher
  9. Tazi M, Dakhlallah D, Caution K, Gerber M, Chang S, Khalil H, et al. Elevated Mirc1/Mir17-92 cluster expression negatively regulates autophagy and CFTR (cystic fibrosis transmembrane conductance regulator) function in CF macrophages. Autophagy. 2016;12:2026-2037 pubmed
  10. Gao Y, Liu Y, Hong L, Yang Z, Cai X, Chen X, et al. Golgi-associated LC3 lipidation requires V-ATPase in noncanonical autophagy. Cell Death Dis. 2016;7:e2330 pubmed publisher
  11. Velázquez R, Shaw D, Caccamo A, Oddo S. Pim1 inhibition as a novel therapeutic strategy for Alzheimer's disease. Mol Neurodegener. 2016;11:52 pubmed publisher
  12. Choi H, Merceron C, Mangiavini L, Seifert E, Schipani E, Shapiro I, et al. Hypoxia promotes noncanonical autophagy in nucleus pulposus cells independent of MTOR and HIF1A signaling. Autophagy. 2016;12:1631-46 pubmed publisher
  13. Shin H, Kim H, Oh S, Lee J, Kee M, Ko H, et al. AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy. Nature. 2016;534:553-7 pubmed publisher
  14. Ting W, Yang J, Kuo C, Xiao Z, Lu X, Yeh Y, et al. Environmental tobacco smoke increases autophagic effects but decreases longevity associated with Sirt-1 protein expression in young C57BL mice hearts. Oncotarget. 2016;7:39017-39025 pubmed publisher
  15. Yeo S, Itahana Y, Guo A, Han R, Iwamoto K, Nguyen H, et al. Transglutaminase 2 contributes to a TP53-induced autophagy program to prevent oncogenic transformation. elife. 2016;5:e07101 pubmed publisher
  16. Kenific C, Stehbens S, Goldsmith J, Leidal A, Faure N, Ye J, et al. NBR1 enables autophagy-dependent focal adhesion turnover. J Cell Biol. 2016;212:577-90 pubmed publisher
  17. Button R, Vincent J, Strang C, Luo S. Dual PI-3 kinase/mTOR inhibition impairs autophagy flux and induces cell death independent of apoptosis and necroptosis. Oncotarget. 2016;7:5157-75 pubmed publisher
  18. Tokuda E, Brännström T, Andersen P, Marklund S. Low autophagy capacity implicated in motor system vulnerability to mutant superoxide dismutase. Acta Neuropathol Commun. 2016;4:6 pubmed publisher
  19. Haim Y, Bluher M, Slutsky N, Goldstein N, Kloting N, Harman Boehm I, et al. Elevated autophagy gene expression in adipose tissue of obese humans: A potential non-cell-cycle-dependent function of E2F1. Autophagy. 2015;11:2074-2088 pubmed publisher
  20. Moreau K, Ghislat G, Hochfeld W, Renna M, Zavodszky E, Runwal G, et al. Transcriptional regulation of Annexin A2 promotes starvation-induced autophagy. Nat Commun. 2015;6:8045 pubmed publisher
  21. Zhang L, Wang H, Ding K, Xu J. FTY720 induces autophagy-related apoptosis and necroptosis in human glioblastoma cells. Toxicol Lett. 2015;236:43-59 pubmed publisher
  22. Luo T, Fu J, Xu A, Su B, Ren Y, Li N, et al. PSMD10/gankyrin induces autophagy to promote tumor progression through cytoplasmic interaction with ATG7 and nuclear transactivation of ATG7 expression. Autophagy. 2016;12:1355-71 pubmed publisher
  23. McMillan E, Paré M, Baechler B, Graham D, Rush J, Quadrilatero J. Autophagic signaling and proteolytic enzyme activity in cardiac and skeletal muscle of spontaneously hypertensive rats following chronic aerobic exercise. PLoS ONE. 2015;10:e0119382 pubmed publisher
  24. Harris White M, Ferbas K, Johnson M, Eslami P, Poteshkina A, Venkova K, et al. A cell-penetrating ester of the neural metabolite lanthionine ketimine stimulates autophagy through the mTORC1 pathway: Evidence for a mechanism of action with pharmacological implications for neurodegenerative pathologies. Neurobiol Dis. 2015;84:60-8 pubmed publisher
  25. Cho S, Yun S, Jo C, Lee D, Choi K, Song J, et al. SUMO1 promotes Aβ production via the modulation of autophagy. Autophagy. 2015;11:100-12 pubmed publisher
  26. Porter K, Hirt J, Stamer W, Liton P. Autophagic dysregulation in glaucomatous trabecular meshwork cells. Biochim Biophys Acta. 2015;1852:379-85 pubmed publisher
  27. Kim T, Kim H, Kang Y, Yoon S, Lee J, Choi W, et al. Psammaplin A induces Sirtuin 1-dependent autophagic cell death in doxorubicin-resistant MCF-7/adr human breast cancer cells and xenografts. Biochim Biophys Acta. 2015;1850:401-10 pubmed publisher
  28. Ortiz F, Acuña Castroviejo D, Doerrier C, Dayoub J, López L, Venegas C, et al. Melatonin blunts the mitochondrial/NLRP3 connection and protects against radiation-induced oral mucositis. J Pineal Res. 2015;58:34-49 pubmed publisher
  29. Chen K, Wang C, Tsai M, Wu C, Yang H, Chen L, et al. Interconnections between autophagy and the coagulation cascade in hepatocellular carcinoma. Cell Death Dis. 2014;5:e1244 pubmed publisher
  30. Porter K, Jeyabalan N, Liton P. MTOR-independent induction of autophagy in trabecular meshwork cells subjected to biaxial stretch. Biochim Biophys Acta. 2014;1843:1054-62 pubmed publisher