This is a Validated Antibody Database (VAD) review about human ASC, based on 26 published articles (read how Labome selects the articles), using ASC antibody in all methods. It is aimed to help Labome visitors find the most suited ASC antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ASC synonym: ASC; CARD5; TMS; TMS-1; TMS1

Knockout validation
MBL International
  • western blot knockout validation; human; loading ...; fig 1a
  • immunocytochemistry; human; 1:600; loading ...; fig 3a, 4c
  • immunocytochemistry; mouse; 1:600; loading ...; fig 4a
MBL International ASC antibody (MBL, D086-3) was used in western blot knockout validation on human samples (fig 1a), in immunocytochemistry on human samples at 1:600 (fig 3a, 4c) and in immunocytochemistry on mouse samples at 1:600 (fig 4a). Proc Natl Acad Sci U S A (2019) ncbi
MBL International
  • western blot knockout validation; human; loading ...; fig s1b
In order to report a role for MLKL in NLRP3 inflammasome activation, MBL International ASC antibody (MBL International, D086-3) was used in western blot knockout validation on human samples (fig s1b). J Immunol (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • immunohistochemistry; pigs ; loading ...; fig 3b
Santa Cruz Biotechnology ASC antibody (Santa Cruz Biotechnology, sc-514414) was used in immunohistochemistry on pigs samples (fig 3b). J Virol (2022) ncbi
mouse monoclonal
  • western blot; mouse; 1:200; loading ...; fig 2a
Santa Cruz Biotechnology ASC antibody (Santa Cruz Biotechnology, sc-514414) was used in western blot on mouse samples at 1:200 (fig 2a). Mol Med Rep (2021) ncbi
mouse monoclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 5a
  • western blot; mouse; 1:1000; loading ...; fig 5b
Santa Cruz Biotechnology ASC antibody (Santa Cruz, sc-514414) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 5a) and in western blot on mouse samples at 1:1000 (fig 5b). Aging (Albany NY) (2021) ncbi
mouse monoclonal (F-9)
  • western blot; mouse; loading ...; fig 4n
Santa Cruz Biotechnology ASC antibody (Santa Cruz Biotechnology, sc-271054) was used in western blot on mouse samples (fig 4n). PLoS Pathog (2020) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig 3e
Santa Cruz Biotechnology ASC antibody (Santa Cruz, B-3) was used in western blot on mouse samples (fig 3e). Sci Rep (2020) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig 5b
Santa Cruz Biotechnology ASC antibody (Santa Cruz, sc-514414) was used in western blot on mouse samples (fig 5b). J Clin Invest (2020) ncbi
mouse monoclonal
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 2b
Santa Cruz Biotechnology ASC antibody (Santa Cruz Biotechnology, sc-514414) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 2b). Cell Death Dis (2020) ncbi
mouse monoclonal
  • western blot; human; 1:1000; loading ...; fig 3a
Santa Cruz Biotechnology ASC antibody (SANTA CRUZ, sc-514414) was used in western blot on human samples at 1:1000 (fig 3a). BMC Cancer (2020) ncbi
mouse monoclonal (F-9)
  • western blot; rat; loading ...; fig 4a
Santa Cruz Biotechnology ASC antibody (Santa Cruz Biotechnology, sc-271054) was used in western blot on rat samples (fig 4a). Biomed Pharmacother (2019) ncbi
mouse monoclonal
  • western blot; human; loading ...; fig 2c, 5a
Santa Cruz Biotechnology ASC antibody (Santa Cruz, sc-514414) was used in western blot on human samples (fig 2c, 5a). J Allergy Clin Immunol (2019) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig 6c
Santa Cruz Biotechnology ASC antibody (Santa Cruz Biotechnology, sc-514414) was used in western blot on mouse samples (fig 6c). Immunity (2019) ncbi
mouse monoclonal
  • western blot; human; 1:100; loading ...; fig 3
Santa Cruz Biotechnology ASC antibody (Santa Cruz, sc-514,414) was used in western blot on human samples at 1:100 (fig 3). J Inflamm (Lond) (2018) ncbi
mouse monoclonal
  • immunoprecipitation; rat; fig 3c
  • western blot; rat; fig 3c
Santa Cruz Biotechnology ASC antibody (Santa Cruz, sc-514414) was used in immunoprecipitation on rat samples (fig 3c) and in western blot on rat samples (fig 3c). Front Cell Neurosci (2017) ncbi
mouse monoclonal (F-9)
  • immunocytochemistry; human; 1:500; loading ...; fig 1b
Santa Cruz Biotechnology ASC antibody (Santa Cruz, sc271054) was used in immunocytochemistry on human samples at 1:500 (fig 1b). Nat Commun (2017) ncbi
mouse monoclonal (F-9)
  • western blot; mouse; loading ...; fig 5e
Santa Cruz Biotechnology ASC antibody (Santa Cruz, sc-271054) was used in western blot on mouse samples (fig 5e). Cell Death Dis (2016) ncbi
mouse monoclonal (F-9)
  • western blot; human; fig 1
Santa Cruz Biotechnology ASC antibody (santa Cruz, sc-271054) was used in western blot on human samples (fig 1). Arthritis Res Ther (2015) ncbi
mouse monoclonal (F-9)
  • immunoprecipitation; human; 1:1000; fig 4
  • western blot; human; 1:1000; fig 4
Santa Cruz Biotechnology ASC antibody (Santa Cruz, sc-271054) was used in immunoprecipitation on human samples at 1:1000 (fig 4) and in western blot on human samples at 1:1000 (fig 4). Biomed Res Int (2015) ncbi
mouse monoclonal (F-9)
  • western blot; human
Santa Cruz Biotechnology ASC antibody (Santa Cruz Biotechnology, sc-271054) was used in western blot on human samples . PLoS Pathog (2014) ncbi
Abcam
domestic rabbit polyclonal
  • western blot; human; 1:2000; loading ...; fig 2a
Abcam ASC antibody (Abcam, ab180799) was used in western blot on human samples at 1:2000 (fig 2a). Aging (Albany NY) (2021) ncbi
domestic goat polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...; fig 8
  • western blot; mouse; 1:1000; loading ...; fig 7a
Abcam ASC antibody (Abcam, ab175449) was used in immunohistochemistry on mouse samples at 1:200 (fig 8) and in western blot on mouse samples at 1:1000 (fig 7a). J Transl Med (2020) ncbi
domestic rabbit monoclonal (EPR10403)
  • western blot; human; 1:1000; loading ...; fig 1c
Abcam ASC antibody (abcam, ab155970) was used in western blot on human samples at 1:1000 (fig 1c). BMC Cardiovasc Disord (2019) ncbi
Novus Biologicals
domestic rabbit polyclonal (8E10.1D6)
Novus Biologicals ASC antibody (Novus Biologicals, NBP1-78977) was used . PLoS ONE (2015) ncbi
BioLegend
mouse monoclonal (HASC-71)
  • flow cytometry; human; loading ...; fig 2f
BioLegend ASC antibody (Biolegend, 653903) was used in flow cytometry on human samples (fig 2f). elife (2019) ncbi
MBL International
  • western blot knockout validation; human; loading ...; fig 1a
  • immunocytochemistry; human; 1:600; loading ...; fig 3a, 4c
  • immunocytochemistry; mouse; 1:600; loading ...; fig 4a
MBL International ASC antibody (MBL, D086-3) was used in western blot knockout validation on human samples (fig 1a), in immunocytochemistry on human samples at 1:600 (fig 3a, 4c) and in immunocytochemistry on mouse samples at 1:600 (fig 4a). Proc Natl Acad Sci U S A (2019) ncbi
  • western blot knockout validation; human; loading ...; fig s1b
In order to report a role for MLKL in NLRP3 inflammasome activation, MBL International ASC antibody (MBL International, D086-3) was used in western blot knockout validation on human samples (fig s1b). J Immunol (2017) ncbi
  • immunocytochemistry; human; 1:100; fig 6
MBL International ASC antibody (MBL, D086-3) was used in immunocytochemistry on human samples at 1:100 (fig 6). PLoS Pathog (2016) ncbi
Articles Reviewed
  1. He S, Li L, Chen H, Hu X, Wang W, Zhang H, et al. PRRSV Infection Induces Gasdermin D-Driven Pyroptosis of Porcine Alveolar Macrophages through NLRP3 Inflammasome Activation. J Virol. 2022;96:e0212721 pubmed publisher
  2. Su Y, Wang Y, Liu M, Chen H. Hydrogen sulfide attenuates renal I/R‑induced activation of the inflammatory response and apoptosis via regulating Nrf2‑mediated NLRP3 signaling pathway inhibition. Mol Med Rep. 2021;24: pubmed publisher
  3. Zhao Z, Wang X, Zhang R, Ma B, Niu S, Di X, et al. Melatonin attenuates smoking-induced atherosclerosis by activating the Nrf2 pathway via NLRP3 inflammasomes in endothelial cells. Aging (Albany NY). 2021;13:11363-11380 pubmed publisher
  4. Ni X, Zhang Y, Jia L, Lu W, Zhu Q, Ren J, et al. Inhibition of Notch1-mediated inflammation by intermedin protects against abdominal aortic aneurysm via PI3K/Akt signaling pathway. Aging (Albany NY). 2021;13:5164-5184 pubmed publisher
  5. Wang W, Hu D, Wu C, Feng Y, Li A, Liu W, et al. STING promotes NLRP3 localization in ER and facilitates NLRP3 deubiquitination to activate the inflammasome upon HSV-1 infection. PLoS Pathog. 2020;16:e1008335 pubmed publisher
  6. Kim Y, Kim J, Gu S, Jo S, Kim S, Young Kim S, et al. Identification of highly potent and selective inhibitor, TIPTP, of the p22phox-Rubicon axis as a therapeutic agent for rheumatoid arthritis. Sci Rep. 2020;10:4570 pubmed publisher
  7. Gao Y, Dai X, Li Y, Li G, Lin X, Ai C, et al. Role of Parkin-mediated mitophagy in the protective effect of polydatin in sepsis-induced acute kidney injury. J Transl Med. 2020;18:114 pubmed publisher
  8. Theivanthiran B, Evans K, Devito N, Plebanek M, Sturdivant M, Wachsmuth L, et al. A tumor-intrinsic PD-L1/NLRP3 inflammasome signaling pathway drives resistance to anti-PD-1 immunotherapy. J Clin Invest. 2020;130:2570-2586 pubmed publisher
  9. Yuan B, Zhou X, You Z, Xu W, Fan J, Chen S, et al. Inhibition of AIM2 inflammasome activation alleviates GSDMD-induced pyroptosis in early brain injury after subarachnoid haemorrhage. Cell Death Dis. 2020;11:76 pubmed publisher
  10. Yang Y, Liu P, Bao W, Chen S, Wu F, Zhu P. Hydrogen inhibits endometrial cancer growth via a ROS/NLRP3/caspase-1/GSDMD-mediated pyroptotic pathway. BMC Cancer. 2020;20:28 pubmed publisher
  11. Liu Y, Tie L. Apolipoprotein M and sphingosine-1-phosphate complex alleviates TNF-α-induced endothelial cell injury and inflammation through PI3K/AKT signaling pathway. BMC Cardiovasc Disord. 2019;19:279 pubmed publisher
  12. Scambler T, Jarosz Griffiths H, Lara Reyna S, Pathak S, Wong C, Holbrook J, et al. ENaC-mediated sodium influx exacerbates NLRP3-dependent inflammation in cystic fibrosis. elife. 2019;8: pubmed publisher
  13. Qin W, Wu X, Jia Y, Tong X, Guo C, Chen D, et al. Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma. Biomed Pharmacother. 2019;118:109188 pubmed publisher
  14. Gordon E, Yao X, Xu H, Karkowsky W, Kaler M, Kalchiem Dekel O, et al. Apolipoprotein E is a concentration-dependent pulmonary danger signal that activates the NLRP3 inflammasome and IL-1β secretion by bronchoalveolar fluid macrophages from asthmatic subjects. J Allergy Clin Immunol. 2019;: pubmed publisher
  15. Davis M, Fairgrieve M, den Hartigh A, Yakovenko O, Duvvuri B, Lood C, et al. Calpain drives pyroptotic vimentin cleavage, intermediate filament loss, and cell rupture that mediates immunostimulation. Proc Natl Acad Sci U S A. 2019;116:5061-5070 pubmed publisher
  16. Arora H, Wilcox S, Johnson L, Munro L, Eyford B, Pfeifer C, et al. The ATP-Binding Cassette Gene ABCF1 Functions as an E2 Ubiquitin-Conjugating Enzyme Controlling Macrophage Polarization to Dampen Lethal Septic Shock. Immunity. 2019;50:418-431.e6 pubmed publisher
  17. Ma Z, Sun H, Lv J, Guo L, Yang Q. Expression and clinical significance of the NEK7-NLRP3 inflammasome signaling pathway in patients with systemic lupus erythematosus. J Inflamm (Lond). 2018;15:16 pubmed publisher
  18. Lin Z, Wang S, Chen L, Zhuang J, Ke Q, Xiao D, et al. Methylene Blue Mitigates Acute Neuroinflammation after Spinal Cord Injury through Inhibiting NLRP3 Inflammasome Activation in Microglia. Front Cell Neurosci. 2017;11:391 pubmed publisher
  19. Bednash J, Weathington N, Londino J, Rojas M, Gulick D, Fort R, et al. Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity. Nat Commun. 2017;8:15203 pubmed publisher
  20. Gutierrez K, Davis M, Daniels B, Olsen T, Ralli Jain P, Tait S, et al. MLKL Activation Triggers NLRP3-Mediated Processing and Release of IL-1β Independently of Gasdermin-D. J Immunol. 2017;198:2156-2164 pubmed publisher
  21. Wei C, Wang Y, Du Z, Guan K, Cao Y, Yang H, et al. The Yersinia Type III secretion effector YopM Is an E3 ubiquitin ligase that induced necrotic cell death by targeting NLRP3. Cell Death Dis. 2016;7:e2519 pubmed publisher
  22. Eichholz K, Bru T, Tran T, Fernandes P, Welles H, Mennechet F, et al. Immune-Complexed Adenovirus Induce AIM2-Mediated Pyroptosis in Human Dendritic Cells. PLoS Pathog. 2016;12:e1005871 pubmed publisher
  23. Yao X, Carlson D, Sun Y, Ma L, Wolf S, Minei J, et al. Mitochondrial ROS Induces Cardiac Inflammation via a Pathway through mtDNA Damage in a Pneumonia-Related Sepsis Model. PLoS ONE. 2015;10:e0139416 pubmed publisher
  24. Choulaki C, Papadaki G, Repa A, Kampouraki E, Kambas K, Ritis K, et al. Enhanced activity of NLRP3 inflammasome in peripheral blood cells of patients with active rheumatoid arthritis. Arthritis Res Ther. 2015;17:257 pubmed publisher
  25. Woolery K, Mohamed M, Linger R, Dobrinski K, Roman J, Kruk P. BRCA1 185delAG Mutation Enhances Interleukin-1β Expression in Ovarian Surface Epithelial Cells. Biomed Res Int. 2015;2015:652017 pubmed publisher
  26. Ataide M, Andrade W, Zamboni D, Wang D, Souza M, Franklin B, et al. Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection. PLoS Pathog. 2014;10:e1003885 pubmed publisher