This is a Validated Antibody Database (VAD) review about human ARNT, based on 30 published articles (read how Labome selects the articles), using ARNT antibody in all methods. It is aimed to help Labome visitors find the most suited ARNT antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ARNT synonym: HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO; bHLHe2

Abcam
mouse monoclonal (2B10)
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 3n
Abcam ARNT antibody (Abcam, ab2771) was used in immunohistochemistry - paraffin section on mouse samples at 1:1000 (fig 3n). iScience (2022) ncbi
domestic rabbit monoclonal (EPR23106-55)
  • western blot; mouse; 1:1000; loading ...; fig 4k
Abcam ARNT antibody (Abcam, ab239366) was used in western blot on mouse samples at 1:1000 (fig 4k). iScience (2022) ncbi
domestic rabbit monoclonal (EPR23106-55)
  • proximity ligation assay; human; loading ...; fig 4b
  • western blot; human; loading ...; fig 4f, 5d
Abcam ARNT antibody (Abcam, ab239366) was used in proximity ligation assay on human samples (fig 4b) and in western blot on human samples (fig 4f, 5d). Oncogene (2021) ncbi
mouse monoclonal (2B10)
  • immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 9c
Abcam ARNT antibody (Abcam, ab2771) was used in immunohistochemistry - paraffin section on rat samples at 1:200 (fig 9c). Front Neurosci (2017) ncbi
Novus Biologicals
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 1d, s2
Novus Biologicals ARNT antibody (Novus, NB100-110) was used in chromatin immunoprecipitation on human samples (fig 1d, s2). Sci Adv (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 3c
Novus Biologicals ARNT antibody (Novus Biologicals, NB100-110) was used in western blot on mouse samples at 1:1000 (fig 3c). Sci Adv (2020) ncbi
mouse monoclonal (H1beta234)
  • western blot; human; loading ...; fig 5f
In order to define distinct functional subgroups of clear cell renal cell carcinoma based on expression of BAF180, Novus Biologicals ARNT antibody (Novus Biologicals, NB100-124) was used in western blot on human samples (fig 5f). Oncogenesis (2017) ncbi
mouse monoclonal (H1beta234)
  • ChIP-Seq; mouse; loading ...; fig s2b
In order to study how tumor hypoxia affects DNA methylation, Novus Biologicals ARNT antibody (Novus, NB100-124) was used in ChIP-Seq on mouse samples (fig s2b). Nature (2016) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; human; loading ...; fig 1a
Novus Biologicals ARNT antibody (Novus, NB-100-110) was used in ChIP-Seq on human samples (fig 1a). EMBO Rep (2016) ncbi
mouse monoclonal (H1beta234)
  • chromatin immunoprecipitation; human; fig 10
In order to determine how prolonged hypoxia conditions are negative regulators of hypoxia-inducible factors by PRDX2 and PRDX4, Novus Biologicals ARNT antibody (Novus Biologicals, NB100-124) was used in chromatin immunoprecipitation on human samples (fig 10). Oncotarget (2016) ncbi
mouse monoclonal (H1beta234)
  • western blot; human; fig 3
In order to study interplay in autophagy regulation between transglutaminase type 2 and pyruvate kinase isoenzyme M2, Novus Biologicals ARNT antibody (Novus Biologicals, NB100-124) was used in western blot on human samples (fig 3). Oncotarget (2015) ncbi
mouse monoclonal (H1beta234)
  • western blot; human
Novus Biologicals ARNT antibody (Novus Biological, NB 100-124) was used in western blot on human samples . Mol Cancer Res (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-3)
  • western blot; human; fig s1c
Santa Cruz Biotechnology ARNT antibody (Santa Cruz, sc-17811) was used in western blot on human samples (fig s1c). J Clin Invest (2018) ncbi
mouse monoclonal (H-10)
  • western blot; mouse; loading ...
Santa Cruz Biotechnology ARNT antibody (Santa Cruz, sc-55526) was used in western blot on mouse samples . Nature (2016) ncbi
mouse monoclonal (G-3)
  • western blot; human; fig 3
In order to study pVHL-mediated degradation of B-Myb and hypoxia-inducible factor alpha by parallele regulation of von Hippel-Lindau disease, Santa Cruz Biotechnology ARNT antibody (Santa Cruz Biotechnology, sc-17812) was used in western blot on human samples (fig 3). Mol Cell Biol (2016) ncbi
mouse monoclonal (A-3)
  • western blot; human
Santa Cruz Biotechnology ARNT antibody (Santa Cruz, sc-17811) was used in western blot on human samples . PLoS ONE (2014) ncbi
Invitrogen
mouse monoclonal (2B10)
  • immunocytochemistry; mouse; loading ...; fig s6a
Invitrogen ARNT antibody (ThermoFisher, MA1-515) was used in immunocytochemistry on mouse samples (fig s6a). Cell (2019) ncbi
mouse monoclonal (2B10)
  • EMSA; mouse
  • EMSA; rat
In order to study the upregulation of pancreatic beta cell ARAP1 by a functional regulatory DNA variant at a type 2 diabetes locus, Invitrogen ARNT antibody (Thermo Scientific, MA1-515) was used in EMSA on mouse samples and in EMSA on rat samples . Am J Hum Genet (2014) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D28F3)
  • chromatin immunoprecipitation; human; loading ...; fig 6l
Cell Signaling Technology ARNT antibody (Cell Signaling, 5537) was used in chromatin immunoprecipitation on human samples (fig 6l). Cell Death Dis (2020) ncbi
domestic rabbit monoclonal (D28F3)
  • immunocytochemistry; mouse; loading ...; fig s6a
Cell Signaling Technology ARNT antibody (Cell Signaling, 5537) was used in immunocytochemistry on mouse samples (fig s6a). Cell (2019) ncbi
domestic rabbit monoclonal (D28F3)
  • western blot; human; 1:1000; loading ...; fig 2e
Cell Signaling Technology ARNT antibody (Cell Signaling, 5531) was used in western blot on human samples at 1:1000 (fig 2e). elife (2019) ncbi
domestic rabbit monoclonal (D28F3)
  • western blot; mouse; 1:1000; loading ...; fig s1a
Cell Signaling Technology ARNT antibody (Cell Signaling Technology, 5537) was used in western blot on mouse samples at 1:1000 (fig s1a). Science (2019) ncbi
domestic rabbit monoclonal (C15A11)
  • western blot; mouse; loading ...; fig s6l
Cell Signaling Technology ARNT antibody (Cell Signaling Technology, 3414) was used in western blot on mouse samples (fig s6l). J Clin Invest (2018) ncbi
domestic rabbit monoclonal (D28F3)
  • western blot; mouse; 1:1000; loading ...; fig 5b
In order to find that CD4+ T cells from DOCK8-deficient mice produce large amounts of IL-31, Cell Signaling Technology ARNT antibody (Cell Signaling, 5537) was used in western blot on mouse samples at 1:1000 (fig 5b). Nat Commun (2017) ncbi
domestic rabbit monoclonal (C15A11)
  • western blot; human; loading ...; fig 4c
Cell Signaling Technology ARNT antibody (cell signalling, 3414) was used in western blot on human samples (fig 4c). Oncotarget (2017) ncbi
domestic rabbit monoclonal (C15A11)
  • western blot; human; 1:1000; loading ...; fig 2a
In order to describe a function for NQO1 in stabilizing HIF-1alpha, Cell Signaling Technology ARNT antibody (Cell Signaling, 3414S) was used in western blot on human samples at 1:1000 (fig 2a). Nat Commun (2016) ncbi
domestic rabbit monoclonal (D28F3)
  • western blot; human; loading ...; fig 6a
In order to report the O-linked N-acetylglucosamine glycoproteome observed in human T cells, Cell Signaling Technology ARNT antibody (Cell Signaling, 5537) was used in western blot on human samples (fig 6a). J Immunol (2016) ncbi
domestic rabbit monoclonal (D28F3)
  • western blot; rat; loading ...; fig 5c
Cell Signaling Technology ARNT antibody (Cell Signaling, 5537) was used in western blot on rat samples (fig 5c). Oncotarget (2016) ncbi
domestic rabbit monoclonal (D28F3)
  • western blot; human; loading ...; fig 3c
In order to identify miR-223 as a negative regulator of the aryl hydrocarbon receptor/aryl hydrocarbon receptor translocator pathway, Cell Signaling Technology ARNT antibody (Cell Signaling, D28F3) was used in western blot on human samples (fig 3c). Sci Rep (2016) ncbi
domestic rabbit monoclonal (C15A11)
  • western blot; human; 1:1000; loading ...; fig 5ba
Cell Signaling Technology ARNT antibody (Cell Signaling, 3414) was used in western blot on human samples at 1:1000 (fig 5ba). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (C15A11)
  • western blot; human
In order to study the molecular signaling responsible for retinoic acid-induced differentiation, Cell Signaling Technology ARNT antibody (Cell Signaling, 3414) was used in western blot on human samples . PLoS ONE (2015) ncbi
domestic rabbit monoclonal (D28F3)
  • western blot; human; 1:1000
  • western blot; mouse; 1:1000
In order to study the effect of allopurinol treatment during hypoxia, Cell Signaling Technology ARNT antibody (Cell Signaling Technology, 5537) was used in western blot on human samples at 1:1000 and in western blot on mouse samples at 1:1000. PLoS ONE (2015) ncbi
Articles Reviewed
  1. Ouyang L, Sun Y, Lv D, Peng X, Liu X, Ci L, et al. miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone. iScience. 2022;25:103604 pubmed publisher
  2. Wang Y, Lyu Y, Tu K, Xu Q, Yang Y, Salman S, et al. Histone citrullination by PADI4 is required for HIF-dependent transcriptional responses to hypoxia and tumor vascularization. Sci Adv. 2021;7: pubmed publisher
  3. Shen M, Zhang R, Jia W, Zhu Z, Zhao X, Zhao L, et al. Nuclear scaffold protein p54nrb/NONO facilitates the hypoxia-enhanced progression of hepatocellular carcinoma. Oncogene. 2021;40:4167-4183 pubmed publisher
  4. Wang S, Qi Y, Gao X, Qiu W, Liu Q, Guo X, et al. Hypoxia-induced lncRNA PDIA3P1 promotes mesenchymal transition via sponging of miR-124-3p in glioma. Cell Death Dis. 2020;11:168 pubmed publisher
  5. Chen J, Haller C, Jernigan F, Koerner S, Wong D, Wang Y, et al. Modulation of lymphocyte-mediated tissue repair by rational design of heterocyclic aryl hydrocarbon receptor agonists. Sci Adv. 2020;6:eaay8230 pubmed publisher
  6. Brigidi G, Hayes M, Delos Santos N, Hartzell A, Texari L, Lin P, et al. Genomic Decoding of Neuronal Depolarization by Stimulus-Specific NPAS4 Heterodimers. Cell. 2019;179:373-391.e27 pubmed publisher
  7. Talreja J, Talwar H, Bauerfeld C, Grossman L, Zhang K, Tranchida P, et al. HIF-1α regulates IL-1β and IL-17 in sarcoidosis. elife. 2019;8: pubmed publisher
  8. Chakraborty A, Laukka T, Myllykoski M, Ringel A, Booker M, Tolstorukov M, et al. Histone demethylase KDM6A directly senses oxygen to control chromatin and cell fate. Science. 2019;363:1217-1222 pubmed publisher
  9. Bianchi Smiraglia A, Bagati A, Fink E, Affronti H, Lipchick B, Moparthy S, et al. Inhibition of the aryl hydrocarbon receptor/polyamine biosynthesis axis suppresses multiple myeloma. J Clin Invest. 2018;128:4682-4696 pubmed publisher
  10. Tan X, Banerjee P, Liu X, Yu J, Gibbons D, Wu P, et al. The epithelial-to-mesenchymal transition activator ZEB1 initiates a prometastatic competing endogenous RNA network. J Clin Invest. 2018;128:1267-1282 pubmed publisher
  11. Chen G, Nie S, Han C, Ma K, Xu Y, Zhang Z, et al. Antidyskinetic Effects of MEK Inhibitor Are Associated with Multiple Neurochemical Alterations in the Striatum of Hemiparkinsonian Rats. Front Neurosci. 2017;11:112 pubmed publisher
  12. Murakami A, Wang L, Kalhorn S, Schraml P, Rathmell W, Tan A, et al. Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma. Oncogenesis. 2017;6:e287 pubmed publisher
  13. Yamamura K, Uruno T, Shiraishi A, Tanaka Y, Ushijima M, Nakahara T, et al. The transcription factor EPAS1 links DOCK8 deficiency to atopic skin inflammation via IL-31 induction. Nat Commun. 2017;8:13946 pubmed publisher
  14. Wang M, Li G, Yang Z, Wang L, Zhang L, Wang T, et al. Uncoupling protein 2 downregulation by hypoxia through repression of peroxisome proliferator-activated receptor γ promotes chemoresistance of non-small cell lung cancer. Oncotarget. 2017;8:8083-8094 pubmed publisher
  15. Oh E, Kim J, Kim J, Kim S, Lee J, Hong S, et al. NQO1 inhibits proteasome-mediated degradation of HIF-1α. Nat Commun. 2016;7:13593 pubmed publisher
  16. Lund P, Elias J, Davis M. Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol. 2016;197:3086-3098 pubmed
  17. Chen W, Hill H, Christie A, Kim M, Holloman E, Pavía Jiménez A, et al. Targeting renal cell carcinoma with a HIF-2 antagonist. Nature. 2016;539:112-117 pubmed publisher
  18. Thienpont B, Steinbacher J, Zhao H, D Anna F, Kuchnio A, Ploumakis A, et al. Tumour hypoxia causes DNA hypermethylation by reducing TET activity. Nature. 2016;537:63-68 pubmed publisher
  19. Platt J, Salama R, Smythies J, Choudhry H, Davies J, Hughes J, et al. Capture-C reveals preformed chromatin interactions between HIF-binding sites and distant promoters. EMBO Rep. 2016;17:1410-1421 pubmed
  20. Okumura F, Uematsu K, Byrne S, Hirano M, Joo Okumura A, Nishikimi A, et al. Parallel Regulation of von Hippel-Lindau Disease by pVHL-Mediated Degradation of B-Myb and Hypoxia-Inducible Factor ?. Mol Cell Biol. 2016;36:1803-17 pubmed publisher
  21. Suyama K, Silagi E, Choi H, Sakabe K, Mochida J, Shapiro I, et al. Circadian factors BMAL1 and ROR? control HIF-1? transcriptional activity in nucleus pulposus cells: implications in maintenance of intervertebral disc health. Oncotarget. 2016;7:23056-71 pubmed publisher
  22. Ogando J, Tardáguila M, Díaz Alderete A, Usategui A, Miranda Ramos V, Martínez Herrera D, et al. Notch-regulated miR-223 targets the aryl hydrocarbon receptor pathway and increases cytokine production in macrophages from rheumatoid arthritis patients. Sci Rep. 2016;6:20223 pubmed publisher
  23. Luo W, Chen I, Chen Y, Alkam D, Wang Y, Semenza G. PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia. Oncotarget. 2016;7:6379-97 pubmed publisher
  24. Altuntas S, Rossin F, Marsella C, D Eletto M, Diaz Hidalgo L, Farrace M, et al. The transglutaminase type 2 and pyruvate kinase isoenzyme M2 interplay in autophagy regulation. Oncotarget. 2015;6:44941-54 pubmed publisher
  25. Wang Y, Wu K, Yang Z, Zhao Q, Fan D, Xu P, et al. Multidrug-Resistance Related Long Non-Coding RNA Expression Profile Analysis of Gastric Cancer. PLoS ONE. 2015;10:e0135461 pubmed publisher
  26. Bunaciu R, Jensen H, Macdonald R, Latocha D, Varner J, Yen A. 6-Formylindolo(3,2-b)Carbazole (FICZ) Modulates the Signalsome Responsible for RA-Induced Differentiation of HL-60 Myeloblastic Leukemia Cells. PLoS ONE. 2015;10:e0135668 pubmed publisher
  27. Sun Y, George J, Rocha S. Dose-dependent effects of allopurinol on human foreskin fibroblast cells and human umbilical vein endothelial cells under hypoxia. PLoS ONE. 2015;10:e0123649 pubmed publisher
  28. Shieh J, Shen C, Chang W, Cheng H, Chan Y, Huang W, et al. An increase in reactive oxygen species by deregulation of ARNT enhances chemotherapeutic drug-induced cancer cell death. PLoS ONE. 2014;9:e99242 pubmed publisher
  29. Sena J, Wang L, Pawlus M, Hu C. HIFs enhance the transcriptional activation and splicing of adrenomedullin. Mol Cancer Res. 2014;12:728-41 pubmed publisher
  30. Kulzer J, Stitzel M, Morken M, Huyghe J, Fuchsberger C, Kuusisto J, et al. A common functional regulatory variant at a type 2 diabetes locus upregulates ARAP1 expression in the pancreatic beta cell. Am J Hum Genet. 2014;94:186-97 pubmed publisher