This is a Validated Antibody Database (VAD) review about human ARHGEF2, based on 8 published articles (read how Labome selects the articles), using ARHGEF2 antibody in all methods. It is aimed to help Labome visitors find the most suited ARHGEF2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ARHGEF2 synonym: GEF; GEF-H1; GEFH1; LFP40; NEDMHM; P40; rho guanine nucleotide exchange factor 2; Rho/Rac guanine nucleotide exchange factor (GEF) 2; guanine nucleotide exchange factor H1; microtubule-regulated Rho-GEF; proliferating cell nucleolar antigen p40

Abcam
mouse monoclonal (B4/7)
  • proximity ligation assay; dog; 1:5; loading ...; fig 7b
  • immunocytochemistry; dog; 1:5; loading ...; fig 7e
  • western blot; dog; 1:50; loading ...; fig 7a
In order to investigate that role of zonula occludens-2 in Rho protein activation and epithelial development, Abcam ARHGEF2 antibody (Abcam, Ab90783) was used in proximity ligation assay on dog samples at 1:5 (fig 7b), in immunocytochemistry on dog samples at 1:5 (fig 7e) and in western blot on dog samples at 1:50 (fig 7a). Biochim Biophys Acta Mol Cell Res (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 6a
  • western blot; human; loading ...; fig 2d
In order to determine the role of cingulin in the barrier function of endothelial cells, Abcam ARHGEF2 antibody (abcam, ab103558) was used in immunohistochemistry - paraffin section on mouse samples (fig 6a) and in western blot on human samples (fig 2d). Arterioscler Thromb Vasc Biol (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 6a
  • western blot; human; loading ...; fig 2d
In order to determine the role of cingulin in the barrier function of endothelial cells, Abcam ARHGEF2 antibody (abcam, ab94348) was used in immunohistochemistry - paraffin section on mouse samples (fig 6a) and in western blot on human samples (fig 2d). Arterioscler Thromb Vasc Biol (2016) ncbi
GeneTex
rabbit polyclonal
  • blocking or activating experiments; human; 1:600; loading ...
  • western blot; human; loading ...; fig 3e
GeneTex ARHGEF2 antibody (GeneTex, GTX125893) was used in blocking or activating experiments on human samples at 1:600 and in western blot on human samples (fig 3e). J Cell Sci (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (55B6)
  • western blot; human; loading ...; fig s6a
Cell Signaling Technology ARHGEF2 antibody (Cell Signaling, 4076) was used in western blot on human samples (fig s6a). Dev Cell (2017) ncbi
rabbit monoclonal (55B6)
  • immunocytochemistry; mouse; loading ...; fig s8
  • western blot; mouse; 1:1000; loading ...; fig s8
  • western blot; African green monkey; 1:1000; loading ...; fig 6d
Cell Signaling Technology ARHGEF2 antibody (cell signalling, 4076) was used in immunocytochemistry on mouse samples (fig s8), in western blot on mouse samples at 1:1000 (fig s8) and in western blot on African green monkey samples at 1:1000 (fig 6d). Nat Commun (2017) ncbi
rabbit monoclonal (55B6)
  • immunoprecipitation; human; 1:1000; loading ...; fig 4c
  • western blot; human; 1:1000; loading ...; fig 4a
Cell Signaling Technology ARHGEF2 antibody (Cell signaling, 55B6) was used in immunoprecipitation on human samples at 1:1000 (fig 4c) and in western blot on human samples at 1:1000 (fig 4a). J Cell Sci (2017) ncbi
rabbit monoclonal (55B6)
  • western blot; human; fig 4
Cell Signaling Technology ARHGEF2 antibody (Cell Signaling, 4076) was used in western blot on human samples (fig 4). Mol Biol Cell (2016) ncbi
rabbit monoclonal (55B6)
  • western blot; human; 1:1000; fig s6
Cell Signaling Technology ARHGEF2 antibody (Cell Signaling, 4076) was used in western blot on human samples at 1:1000 (fig s6). Nat Commun (2016) ncbi
Articles Reviewed
  1. Vassilev V, Platek A, Hiver S, Enomoto H, Takeichi M. Catenins Steer Cell Migration via Stabilization of Front-Rear Polarity. Dev Cell. 2017;43:463-479.e5 pubmed publisher
  2. Takano T, Wu M, Nakamuta S, Naoki H, Ishizawa N, Namba T, et al. Discovery of long-range inhibitory signaling to ensure single axon formation. Nat Commun. 2017;8:33 pubmed publisher
  3. Raya Sandino A, Castillo Kauil A, Domínguez Calderón A, Alarcón L, Flores Benitez D, Cuellar Perez F, et al. Zonula occludens-2 regulates Rho proteins activity and the development of epithelial cytoarchitecture and barrier function. Biochim Biophys Acta Mol Cell Res. 2017;1864:1714-1733 pubmed publisher
  4. Jiu Y, Peranen J, Schaible N, Cheng F, Eriksson J, Krishnan R, et al. Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA. J Cell Sci. 2017;130:892-902 pubmed publisher
  5. Scott D, Tolbert C, Burridge K. Tension on JAM-A activates RhoA via GEF-H1 and p115 RhoGEF. Mol Biol Cell. 2016;27:1420-30 pubmed publisher
  6. Schossleitner K, Rauscher S, Gröger M, Friedl H, Finsterwalder R, Habertheuer A, et al. Evidence That Cingulin Regulates Endothelial Barrier Function In Vitro and In Vivo. Arterioscler Thromb Vasc Biol. 2016;36:647-54 pubmed publisher
  7. Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, et al. F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling. Nat Commun. 2016;7:10493 pubmed publisher
  8. Wang H, Yang C, Lee H, Kuo J, Hur S, Chien S, et al. Dexamethasone-induced cellular tension requires a SGK1-stimulated Sec5-GEF-H1 interaction. J Cell Sci. 2015;128:3757-68 pubmed publisher