This is a Validated Antibody Database (VAD) review about human ARAF, based on 6 published articles (read how Labome selects the articles), using ARAF antibody in all methods. It is aimed to help Labome visitors find the most suited ARAF antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ARAF synonym: A-RAF; ARAF1; PKS2; RAFA1; serine/threonine-protein kinase A-Raf; A-Raf proto-oncogene serine/threonine-protein kinase; Oncogene ARAF1; Ras-binding protein DA-Raf; proto-oncogene A-Raf-1; proto-oncogene Pks; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene-like protein

Abcam
rabbit monoclonal (EPR16208)
  • immunohistochemistry - paraffin section; mouse; 1:700; fig 4
In order to elucidate a screening that identifies kinases involved in prostate cancer visceral and bone metastasis, Abcam ARAF antibody (Abcam, ab200653) was used in immunohistochemistry - paraffin section on mouse samples at 1:700 (fig 4). Proc Natl Acad Sci U S A (2016) ncbi
Santa Cruz Biotechnology
mouse monoclonal (1)
  • proximity ligation assay; hamsters; loading ...; fig 3d
In order to clarify the interaction of SPRED1 with Ras and Raf, Santa Cruz Biotechnology ARAF antibody (Santa Cruz, sc-135820) was used in proximity ligation assay on hamsters samples (fig 3d). Mol Cell Biol (2016) ncbi
Cell Signaling Technology
rabbit polyclonal
  • other; human; loading ...; fig 4c
Cell Signaling Technology ARAF antibody (Cell Signaling, 4432) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
rabbit polyclonal
  • reverse phase protein lysate microarray; human; loading ...; fig st6
In order to characterize the molecular identity of uterine carcinosarcomas., Cell Signaling Technology ARAF antibody (CST, 4431) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 4a
In order to assess the effects of LY3009120, a panRAF and RAF dimer inhibitor, in human models of colorectal cancer, Cell Signaling Technology ARAF antibody (Cell Signaling, 4432) was used in western blot on human samples (fig 4a). Oncotarget (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 3b
Cell Signaling Technology ARAF antibody (Cell Signaling, 4431) was used in western blot on human samples at 1:500 (fig 3b). Am J Physiol Lung Cell Mol Physiol (2016) ncbi
Articles Reviewed
  1. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  2. Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
  3. Vakana E, Pratt S, Blosser W, Dowless M, Simpson N, Yuan X, et al. LY3009120, a panRAF inhibitor, has significant anti-tumor activity in BRAF and KRAS mutant preclinical models of colorectal cancer. Oncotarget. 2017;8:9251-9266 pubmed publisher
  4. Siljamäki E, Abankwa D. SPRED1 Interferes with K-ras but Not H-ras Membrane Anchorage and Signaling. Mol Cell Biol. 2016;36:2612-25 pubmed publisher
  5. Faltermeier C, Drake J, Clark P, Smith B, Zong Y, Volpe C, et al. Functional screen identifies kinases driving prostate cancer visceral and bone metastasis. Proc Natl Acad Sci U S A. 2016;113:E172-81 pubmed publisher
  6. Awad K, Elinoff J, Wang S, Gairhe S, Ferreyra G, Cai R, et al. Raf/ERK drives the proliferative and invasive phenotype of BMPR2-silenced pulmonary artery endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2016;310:L187-201 pubmed publisher