This is a Validated Antibody Database (VAD) review about human APEX1, based on 18 published articles (read how Labome selects the articles), using APEX1 antibody in all methods. It is aimed to help Labome visitors find the most suited APEX1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
APEX1 synonym: APE; APE1; APEN; APEX; APX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase; AP endonuclease class I; AP lyase; APEX nuclease (multifunctional DNA repair enzyme) 1; apurinic-apyrimidinic endonuclease 1; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic); protein REF-1; redox factor-1

Knockout validation
Abcam
rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 4b
  • immunohistochemistry - paraffin section; human; loading ...; fig s7
  • western blot; human; fig 2c
Abcam APEX1 antibody (Abcam, ab137708) was used in western blot knockout validation on mouse samples (fig 4b), in immunohistochemistry - paraffin section on human samples (fig s7) and in western blot on human samples (fig 2c). Nucleic Acids Res (2018) ncbi
Novus Biologicals
mouse monoclonal (13B8E5C2)
  • western blot; human; loading ...; fig 4c
Novus Biologicals APEX1 antibody (Novus Biologicals, NB100-116) was used in western blot on human samples (fig 4c). Nucleic Acids Res (2017) ncbi
mouse monoclonal (13B8E5C2)
  • western blot; mouse; fig 5e
Novus Biologicals APEX1 antibody (Novus Biologicals, NB100-116) was used in western blot on mouse samples (fig 5e). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:2000; fig 3
Novus Biologicals APEX1 antibody (Novus Biologicals, NB100-101) was used in immunocytochemistry on human samples at 1:2000 (fig 3). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 0.1 ug/ml; fig st1
  • western blot; human; fig 3
Novus Biologicals APEX1 antibody (Novus Biologicals, NB100-101) was used in immunohistochemistry - paraffin section on human samples at 0.1 ug/ml (fig st1) and in western blot on human samples (fig 3). Nature (2016) ncbi
mouse monoclonal (13B8E5C2)
  • western blot; mouse; fig s6
Novus Biologicals APEX1 antibody (Novus, NB100-116) was used in western blot on mouse samples (fig s6). Nat Commun (2016) ncbi
mouse monoclonal (13B8E5C2)
  • chromatin immunoprecipitation; human
Novus Biologicals APEX1 antibody (Novus Biologicals, NB100-116) was used in chromatin immunoprecipitation on human samples . Prostate (2015) ncbi
Abcam
rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 4b
  • immunohistochemistry - paraffin section; human; loading ...; fig s7
  • western blot; human; fig 2c
Abcam APEX1 antibody (Abcam, ab137708) was used in western blot knockout validation on mouse samples (fig 4b), in immunohistochemistry - paraffin section on human samples (fig s7) and in western blot on human samples (fig 2c). Nucleic Acids Res (2018) ncbi
mouse monoclonal (13B8E5C2)
  • western blot; human; 1:2000; fig 2b
Abcam APEX1 antibody (Abcam, ab194) was used in western blot on human samples at 1:2000 (fig 2b). Genome Biol (2018) ncbi
rabbit monoclonal (EPR4022)
  • western blot; human; fig 1
Abcam APEX1 antibody (Epitomics, 2851-1) was used in western blot on human samples (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (13B8E5C2)
  • western blot; human; 1:2000; fig 2
Abcam APEX1 antibody (Abcam, ab194) was used in western blot on human samples at 1:2000 (fig 2). PLoS ONE (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (H-6)
  • western blot; human; loading ...; fig s2b
Santa Cruz Biotechnology APEX1 antibody (SantaCruz, sc-55498) was used in western blot on human samples (fig s2b). Oncogene (2017) ncbi
mouse monoclonal (H-6)
  • proximity ligation assay; human; 1:250; fig 2
  • immunohistochemistry - paraffin section; human; fig 5
  • western blot; human; fig 1
In order to study how Parkin is responsible for polyubiquitination of apurinic/apyrimidinic endonuclease 1, Santa Cruz Biotechnology APEX1 antibody (Santa Cruz, sc-55498) was used in proximity ligation assay on human samples at 1:250 (fig 2), in immunohistochemistry - paraffin section on human samples (fig 5) and in western blot on human samples (fig 1). Mol Carcinog (2017) ncbi
mouse monoclonal (H-6)
  • western blot; human; fig 6
Santa Cruz Biotechnology APEX1 antibody (SCBT, sc-55498) was used in western blot on human samples (fig 6). DNA Repair (Amst) (2015) ncbi
MilliporeSigma
mouse monoclonal (APEREF)
  • western blot; mouse; fig 2
MilliporeSigma APEX1 antibody (Sigma-Aldrich, A2105) was used in western blot on mouse samples (fig 2). PLoS ONE (2015) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; loading ...; fig 1b
  • western blot; rat; loading ...; fig 7f
Cell Signaling Technology APEX1 antibody (Cell Signaling, 4128) was used in western blot on human samples (fig 1b) and in western blot on rat samples (fig 7f). elife (2019) ncbi
rabbit polyclonal
  • flow cytometry; human; fig 3
Cell Signaling Technology APEX1 antibody (Cell Signaling Technology, 4128) was used in flow cytometry on human samples (fig 3). Sci Rep (2016) ncbi
rabbit polyclonal
  • reverse phase protein lysate microarray; mouse; fig s1.b,c
Cell Signaling Technology APEX1 antibody (Cell Signaling Technology, 4128) was used in reverse phase protein lysate microarray on mouse samples (fig s1.b,c). EMBO Mol Med (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 3a
In order to study quinacrine-induced apoptosis, Cell Signaling Technology APEX1 antibody (Cell Signaling, 4128) was used in western blot on human samples (fig 3a). Biochem Pharmacol (2016) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology APEX1 antibody (Cell Signaling Technology, 4128) was used in western blot on human samples . elife (2014) ncbi
BD Biosciences
mouse monoclonal (15/Ref-1)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences APEX1 antibody (BD, 15) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
Articles Reviewed
  1. Gao R, Chakraborty A, Geater C, Pradhan S, Gordon K, Snowden J, et al. Mutant huntingtin impairs PNKP and ATXN3, disrupting DNA repair and transcription. elife. 2019;8: pubmed publisher
  2. Li M, Yang X, LU X, Dai N, Zhang S, Cheng Y, et al. APE1 deficiency promotes cellular senescence and premature aging features. Nucleic Acids Res. 2018;46:5664-5677 pubmed publisher
  3. Galanos P, Pappas G, Polyzos A, Kotsinas A, Svolaki I, Giakoumakis N, et al. Mutational signatures reveal the role of RAD52 in p53-independent p21-driven genomic instability. Genome Biol. 2018;19:37 pubmed publisher
  4. Yang C, Sengupta S, Hegde P, Mitra J, Jiang S, Holey B, et al. Regulation of oxidized base damage repair by chromatin assembly factor 1 subunit A. Nucleic Acids Res. 2017;45:739-748 pubmed publisher
  5. Amenya H, Tohyama C, Ohsako S. Dioxin induces Ahr-dependent robust DNA demethylation of the Cyp1a1 promoter via Tdg in the mouse liver. Sci Rep. 2016;6:34989 pubmed publisher
  6. Nassour H, Wang Z, Saad A, Papaluca A, Brosseau N, Affar E, et al. Peroxiredoxin 1 interacts with and blocks the redox factor APE1 from activating interleukin-8 expression. Sci Rep. 2016;6:29389 pubmed publisher
  7. Sun H, He L, Wu H, Pan F, Wu X, Zhao J, et al. The FEN1 L209P mutation interferes with long-patch base excision repair and induces cellular transformation. Oncogene. 2017;36:194-207 pubmed publisher
  8. Scott T, Wicker C, Suganya R, Dhar B, Pittman T, Horbinski C, et al. Polyubiquitination of apurinic/apyrimidinic endonuclease 1 by Parkin. Mol Carcinog. 2017;56:325-336 pubmed publisher
  9. Kaur A, Webster M, Marchbank K, Behera R, Ndoye A, Kugel C, et al. sFRP2 in the aged microenvironment drives melanoma metastasis and therapy resistance. Nature. 2016;532:250-4 pubmed publisher
  10. Gruosso T, Mieulet V, Cardon M, Bourachot B, Kieffer Y, Devun F, et al. Chronic oxidative stress promotes H2AX protein degradation and enhances chemosensitivity in breast cancer patients. EMBO Mol Med. 2016;8:527-49 pubmed publisher
  11. Ahuja A, Jodkowska K, Teloni F, Bizard A, Zellweger R, Herrador R, et al. A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells. Nat Commun. 2016;7:10660 pubmed publisher
  12. Preet R, Siddharth S, Satapathy S, Das S, Nayak A, Das D, et al. Chk1 inhibitor synergizes quinacrine mediated apoptosis in breast cancer cells by compromising the base excision repair cascade. Biochem Pharmacol. 2016;105:23-33 pubmed publisher
  13. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  14. Sassa A, Kamoshita N, Kanemaru Y, Honma M, Yasui M. Xeroderma Pigmentosum Group A Suppresses Mutagenesis Caused by Clustered Oxidative DNA Adducts in the Human Genome. PLoS ONE. 2015;10:e0142218 pubmed publisher
  15. Yao X, Carlson D, Sun Y, Ma L, Wolf S, Minei J, et al. Mitochondrial ROS Induces Cardiac Inflammation via a Pathway through mtDNA Damage in a Pneumonia-Related Sepsis Model. PLoS ONE. 2015;10:e0139416 pubmed publisher
  16. Yang S, Zhang J, Zhang Y, Wan X, Zhang C, Huang X, et al. KDM1A triggers androgen-induced miRNA transcription via H3K4me2 demethylation and DNA oxidation. Prostate. 2015;75:936-46 pubmed publisher
  17. Suganya R, Chakraborty A, Miriyala S, Hazra T, Izumi T. Suppression of oxidative phosphorylation in mouse embryonic fibroblast cells deficient in apurinic/apyrimidinic endonuclease. DNA Repair (Amst). 2015;27:40-8 pubmed publisher
  18. Chen J, Miller B, Furano A. Repair of naturally occurring mismatches can induce mutations in flanking DNA. elife. 2014;3:e02001 pubmed publisher