This is a Validated Antibody Database (VAD) review about human AP-2, based on 40 published articles (read how Labome selects the articles), using AP-2 antibody in all methods. It is aimed to help Labome visitors find the most suited AP-2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
AP-2 synonym: AP-2; AP-2alpha; AP2TF; BOFS; TFAP2

Knockout validation
Developmental Studies Hybridoma Bank
mouse monoclonal (3B5)
  • immunocytochemistry knockout validation; human; loading ...; fig 5a
In order to research a paradigm for generation of all major ectodermal lineages from human pluripotent stem cells, Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5) was used in immunocytochemistry knockout validation on human samples (fig 5a). Cell Stem Cell (2017) ncbi
Abcam
domestic rabbit monoclonal (EPR2688(2))
  • immunocytochemistry; mouse; 1:500; loading ...; fig 2b
Abcam AP-2 antibody (Abcam, ab108311) was used in immunocytochemistry on mouse samples at 1:500 (fig 2b). Sci Adv (2021) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; human; loading ...; fig 4a
Abcam AP-2 antibody (Abcam, ab52222) was used in chromatin immunoprecipitation on human samples (fig 4a). Int J Biol Sci (2020) ncbi
domestic rabbit monoclonal (EPR2688(2))
  • western blot; human; loading ...
In order to research a paradigm for generation of all major ectodermal lineages from human pluripotent stem cells, Abcam AP-2 antibody (Abcam, ab108311) was used in western blot on human samples . Cell Stem Cell (2017) ncbi
domestic rabbit monoclonal (EPR2688(2))
  • western blot; human; fig 1a
Abcam AP-2 antibody (Abcam, ab108311) was used in western blot on human samples (fig 1a). Stem Cell Reports (2016) ncbi
domestic rabbit monoclonal (EPR2688(2))
  • immunohistochemistry; mouse; 1:400; loading ...; fig 1a
In order to report a role for Tfrc in craniofacial development and perhaps Pierre Robin Sequence, Abcam AP-2 antibody (Epitomics, 3154-1) was used in immunohistochemistry on mouse samples at 1:400 (fig 1a). Cell Death Dis (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; fig 2
Abcam AP-2 antibody (Abcam, ab52222) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 2). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:800; fig s6
Abcam AP-2 antibody (Abcam, ab52222) was used in immunocytochemistry on human samples at 1:800 (fig s6). Nat Commun (2016) ncbi
domestic rabbit monoclonal (EPR2688(2))
  • western blot; human; fig 2d
Abcam AP-2 antibody (Epitomics, 3154-1) was used in western blot on human samples (fig 2d). Oncogene (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (3B5)
  • immunocytochemistry; human; loading ...; fig s3a
  • western blot; human; loading ...; fig 2e
Santa Cruz Biotechnology AP-2 antibody (Santa, sc-12726) was used in immunocytochemistry on human samples (fig s3a) and in western blot on human samples (fig 2e). Cell (2019) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry; human; 1:100; loading ...; fig 2s1e
Santa Cruz Biotechnology AP-2 antibody (Santa Cruz, sc-12726) was used in immunohistochemistry on human samples at 1:100 (fig 2s1e). elife (2019) ncbi
mouse monoclonal (3B5)
  • immunocytochemistry; human; loading ...; fig 3f
Santa Cruz Biotechnology AP-2 antibody (Santa Cruz Biotechnology, sc-12726) was used in immunocytochemistry on human samples (fig 3f). Cell Stem Cell (2019) ncbi
mouse monoclonal (3H547)
  • western blot; human; 1:2000; loading ...; fig 1a
Santa Cruz Biotechnology AP-2 antibody (Santa Cruz, sc-70361) was used in western blot on human samples at 1:2000 (fig 1a). Apoptosis (2017) ncbi
mouse monoclonal (8G8/5)
  • immunocytochemistry; human; 1:100; fig 2
Santa Cruz Biotechnology AP-2 antibody (santa Cruz, sc-53164) was used in immunocytochemistry on human samples at 1:100 (fig 2). Cytotechnology (2016) ncbi
mouse monoclonal (3B5)
  • immunocytochemistry; human
In order to study the role of Rho-associated kinase and its downstream target nonmuscle Myosin II in neural crest specification, Santa Cruz Biotechnology AP-2 antibody (Santa Cruz, SC-12726) was used in immunocytochemistry on human samples . Stem Cells (2015) ncbi
Invitrogen
mouse monoclonal (3B5)
  • immunocytochemistry; human; 1:400; loading ...; fig 2e
Invitrogen AP-2 antibody (Thermo Fisher Scientific, MA1-872) was used in immunocytochemistry on human samples at 1:400 (fig 2e). Cells (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C83E10)
  • western blot; mouse; 1:1000; loading ...; fig 6g
Cell Signaling Technology AP-2 antibody (CST, 3215) was used in western blot on mouse samples at 1:1000 (fig 6g). Nat Commun (2022) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:100; fig 3i
Cell Signaling Technology AP-2 antibody (CST, 3208) was used in immunocytochemistry on mouse samples at 1:100 (fig 3i). Commun Biol (2022) ncbi
domestic rabbit monoclonal (C83E10)
  • immunohistochemistry - paraffin section; mouse
  • western blot; mouse; 1:1000
In order to study the development of fatal cardiac hypertrophy and arrrhythmia in mice overexpressing miRNA-17-92 in heart and smooth muscle, Cell Signaling Technology AP-2 antibody (Cell Signaling Technology, 3215) was used in immunohistochemistry - paraffin section on mouse samples and in western blot on mouse samples at 1:1000. FASEB J (2013) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (3B5)
  • immunohistochemistry - frozen section; chicken; 1:200; loading ...; fig s6a
Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5) was used in immunohistochemistry - frozen section on chicken samples at 1:200 (fig s6a). Sci Rep (2021) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3c
Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5) was used in immunohistochemistry - frozen section on mouse samples (fig 3c). Invest Ophthalmol Vis Sci (2021) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry; mouse; 1:20; loading ...; fig 8q
Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5) was used in immunohistochemistry on mouse samples at 1:20 (fig 8q). BMC Biol (2021) ncbi
mouse monoclonal (3B5)
  • immunocytochemistry; human; 1:100; fig 1g
Developmental Studies Hybridoma Bank AP-2 antibody (DHSB, 3B5) was used in immunocytochemistry on human samples at 1:100 (fig 1g). Cell Transplant (2021) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry; chicken; 1:100; loading ...; fig 6d
Developmental Studies Hybridoma Bank AP-2 antibody (Developmental Studies Hybridoma Bank, 3B5) was used in immunohistochemistry on chicken samples at 1:100 (fig 6d). elife (2021) ncbi
mouse monoclonal (3B5)
  • immunocytochemistry; human; fig 1c
Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5) was used in immunocytochemistry on human samples (fig 1c). J Clin Invest (2020) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 2b, 2c
Developmental Studies Hybridoma Bank AP-2 antibody (Developmental Studies Hybridoma Bank, 3b5) was used in immunohistochemistry on mouse samples at 1:100 (fig 2b, 2c). Genesis (2020) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry - frozen section; chicken; 1:200; loading ...; fig s8c
Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5) was used in immunohistochemistry - frozen section on chicken samples at 1:200 (fig s8c). Sci Rep (2019) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry - frozen section; mouse; 1:20; loading ...; fig 6h
Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5 supernatant) was used in immunohistochemistry - frozen section on mouse samples at 1:20 (fig 6h). elife (2019) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry - frozen section; chicken; 1:1000; loading ...; fig 3a
Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5-c) was used in immunohistochemistry - frozen section on chicken samples at 1:1000 (fig 3a). Biol Open (2019) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry - paraffin section; mouse; 1:400; loading ...; fig 3
  • western blot; human; loading ...; fig 2a
Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5) was used in immunohistochemistry - paraffin section on mouse samples at 1:400 (fig 3) and in western blot on human samples (fig 2a). Sci Rep (2018) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry; tree shrew; 1:500; loading ...; fig 6i
Developmental Studies Hybridoma Bank AP-2 antibody (Developmental Studies Hybridoma Bank, 3B5) was used in immunohistochemistry on tree shrew samples at 1:500 (fig 6i). J Comp Neurol (2019) ncbi
mouse monoclonal (3B5)
  • immunocytochemistry knockout validation; human; loading ...; fig 5a
In order to research a paradigm for generation of all major ectodermal lineages from human pluripotent stem cells, Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5) was used in immunocytochemistry knockout validation on human samples (fig 5a). Cell Stem Cell (2017) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry; mouse; 1:50; loading ...; fig 1b
Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5) was used in immunohistochemistry on mouse samples at 1:50 (fig 1b). Science (2017) ncbi
mouse monoclonal (3B5)
  • immunocytochemistry; human; 1:100; loading ...; fig 1c
  • immunohistochemistry; human; 1:100; fig 6a
Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5) was used in immunocytochemistry on human samples at 1:100 (fig 1c) and in immunohistochemistry on human samples at 1:100 (fig 6a). PLoS ONE (2016) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry - frozen section; mouse; 1:50; fig 3
Developmental Studies Hybridoma Bank AP-2 antibody (Developmental Studies Hybridoma Bank, 3B5) was used in immunohistochemistry - frozen section on mouse samples at 1:50 (fig 3). Mol Vis (2016) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry; mouse; 1:250; fig 3
Developmental Studies Hybridoma Bank AP-2 antibody (Developmental Studies Hybridoma Bank, 3B5) was used in immunohistochemistry on mouse samples at 1:250 (fig 3). Mol Brain (2016) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry; mouse; fig s2
Developmental Studies Hybridoma Bank AP-2 antibody (DSHB, 3B5) was used in immunohistochemistry on mouse samples (fig s2). Nature (2015) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry - frozen section; rat
Developmental Studies Hybridoma Bank AP-2 antibody (Developmental Studies Hybridoma Bank, 3B5) was used in immunohistochemistry - frozen section on rat samples . PLoS ONE (2013) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry; chicken
Developmental Studies Hybridoma Bank AP-2 antibody (Developmental Studies Hybridoma Bank, 3B5) was used in immunohistochemistry on chicken samples . J Mol Neurosci (2013) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry - paraffin section; mouse; 1:50
Developmental Studies Hybridoma Bank AP-2 antibody (Developmental Studies Hybridoma Bank, 3B5) was used in immunohistochemistry - paraffin section on mouse samples at 1:50. Cilia (2012) ncbi
mouse monoclonal (3B5)
  • immunocytochemistry; chicken; 1:50
Developmental Studies Hybridoma Bank AP-2 antibody (Developmental Studies Hybridoma Bank, 3B5) was used in immunocytochemistry on chicken samples at 1:50. J Comp Neurol (2010) ncbi
mouse monoclonal (3B5)
  • immunohistochemistry - frozen section; chicken; 1:100
Developmental Studies Hybridoma Bank AP-2 antibody (Developmental Studies Hybridoma Bank, 3B5) was used in immunohistochemistry - frozen section on chicken samples at 1:100. J Comp Neurol (2007) ncbi
Articles Reviewed
  1. Liu H, He J, Bagheri Yarmand R, Li Z, Liu R, Wang Z, et al. Osteocyte CIITA aggravates osteolytic bone lesions in myeloma. Nat Commun. 2022;13:3684 pubmed publisher
  2. Kimura Yoshida C, Mochida K, Kanno S, Matsuo I. USP39 is essential for mammalian epithelial morphogenesis through upregulation of planar cell polarity components. Commun Biol. 2022;5:378 pubmed publisher
  3. Chen X, Emerson M. Notch signaling represses cone photoreceptor formation through the regulation of retinal progenitor cell states. Sci Rep. 2021;11:14525 pubmed publisher
  4. Pan S, Zhao N, Feng X, Jie Y, Jin Z. Conversion of mouse embryonic fibroblasts into neural crest cells and functional corneal endothelia by defined small molecules. Sci Adv. 2021;7: pubmed publisher
  5. Deng X, Iwagawa T, Fukushima M, Suzuki Y, Watanabe S. Setd1a Plays Pivotal Roles for the Survival and Proliferation of Retinal Progenitors via Histone Modifications of Uhrf1. Invest Ophthalmol Vis Sci. 2021;62:1 pubmed publisher
  6. Ofek S, Wiszniak S, Kagan S, Tondl M, Schwarz Q, Kalcheim C. Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube. BMC Biol. 2021;19:84 pubmed publisher
  7. Grönroos P, Ilmarinen T, Skottman H. Directed Differentiation of Human Pluripotent Stem Cells towards Corneal Endothelial-Like Cells under Defined Conditions. Cells. 2021;10: pubmed publisher
  8. Jones I, Novikova L, Wiberg M, Carlsson L, Novikov L. Human Embryonic Stem Cell-derived Neural Crest Cells Promote Sprouting and Motor Recovery Following Spinal Cord Injury in Adult Rats. Cell Transplant. 2021;30:963689720988245 pubmed publisher
  9. Yamagata M, Yan W, Sanes J. A cell atlas of the chick retina based on single-cell transcriptomics. elife. 2021;10: pubmed publisher
  10. Mo J, Anastasaki C, Chen Z, Shipman T, Papke J, Yin K, et al. Humanized neurofibroma model from induced pluripotent stem cells delineates tumor pathogenesis and developmental origins. J Clin Invest. 2020;: pubmed publisher
  11. Sanchez J, Miyake R, Cheng A, Liu T, Iseki S, Kume T. Conditional inactivation of Foxc1 and Foxc2 in neural crest cells leads to cardiac abnormalities. Genesis. 2020;58:e23364 pubmed publisher
  12. Guoren Z, Zhaohui F, Wei Z, Mei W, Yuan W, Lin S, et al. TFAP2A Induced ITPKA Serves as an Oncogene and Interacts with DBN1 in Lung Adenocarcinoma. Int J Biol Sci. 2020;16:504-514 pubmed publisher
  13. Greenberg R, Long H, Swigut T, Wysocka J. Single Amino Acid Change Underlies Distinct Roles of H2A.Z Subtypes in Human Syndrome. Cell. 2019;178:1421-1436.e24 pubmed publisher
  14. Achberger K, Probst C, Haderspeck J, Bolz S, Rogal J, Chuchuy J, et al. Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform. elife. 2019;8: pubmed publisher
  15. Schick E, McCaffery S, Keblish E, Thakurdin C, Emerson M. Lineage tracing analysis of cone photoreceptor associated cis-regulatory elements in the developing chicken retina. Sci Rep. 2019;9:9358 pubmed publisher
  16. Lukacs M, ROBERTS T, Chatuverdi P, Stottmann R. Glycosylphosphatidylinositol biosynthesis and remodeling are required for neural tube closure, heart development, and cranial neural crest cell survival. elife. 2019;8: pubmed publisher
  17. Laugsch M, Bartusel M, Rehimi R, Alirzayeva H, Karaolidou A, Crispatzu G, et al. Modeling the Pathological Long-Range Regulatory Effects of Human Structural Variation with Patient-Specific hiPSCs. Cell Stem Cell. 2019;24:736-752.e12 pubmed publisher
  18. Martin J, Muccioli M, Herman K, Finnell R, Plageman T. Folic acid modifies the shape of epithelial cells during morphogenesis via a Folr1 and MLCK dependent mechanism. Biol Open. 2019;8: pubmed publisher
  19. Jain S, Glubrecht D, Germain D, Moser M, Godbout R. AP-2ε Expression in Developing Retina: Contributing to the Molecular Diversity of Amacrine Cells. Sci Rep. 2018;8:3386 pubmed publisher
  20. Johnson E, Westbrook T, Shayesteh R, Chen E, Schumacher J, Fitzpatrick D, et al. Distribution and diversity of intrinsically photosensitive retinal ganglion cells in tree shrew. J Comp Neurol. 2019;527:328-344 pubmed publisher
  21. Tchieu J, Zimmer B, Fattahi F, Amin S, Zeltner N, Chen S, et al. A Modular Platform for Differentiation of Human PSCs into All Major Ectodermal Lineages. Cell Stem Cell. 2017;21:399-410.e7 pubmed publisher
  22. Zhao F, Franco H, Rodriguez K, Brown P, Tsai M, Tsai S, et al. Elimination of the male reproductive tract in the female embryo is promoted by COUP-TFII in mice. Science. 2017;357:717-720 pubmed publisher
  23. Lian W, Zhang L, Yang L, Chen W. AP-2α reverses vincristine-induced multidrug resistance of SGC7901 gastric cancer cells by inhibiting the Notch pathway. Apoptosis. 2017;22:933-941 pubmed publisher
  24. Bogachek M, Park J, De Andrade J, Lorenzen A, Kulak M, White J, et al. Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas. Stem Cell Reports. 2016;7:1140-1151 pubmed publisher
  25. Naylor R, McGhee C, Cowan C, Davidson A, Holm T, Sherwin T. Derivation of Corneal Keratocyte-Like Cells from Human Induced Pluripotent Stem Cells. PLoS ONE. 2016;11:e0165464 pubmed publisher
  26. Simmons A, Bloomsburg S, Billingslea S, Merrill M, Li S, Thomas M, et al. Pou4f2 knock-in Cre mouse: A multifaceted genetic tool for vision researchers. Mol Vis. 2016;22:705-17 pubmed
  27. Lei R, Zhang K, Liu K, Shao X, Ding Z, Wang F, et al. Transferrin receptor facilitates TGF-β and BMP signaling activation to control craniofacial morphogenesis. Cell Death Dis. 2016;7:e2282 pubmed publisher
  28. Li X, Gaillard F, Monckton E, Glubrecht D, Persad A, Moser M, et al. Loss of AP-2delta reduces retinal ganglion cell numbers and axonal projections to the superior colliculus. Mol Brain. 2016;9:62 pubmed publisher
  29. Balakrishnan A, Stykel M, Touahri Y, Stratton J, Biernaskie J, Schuurmans C. Temporal Analysis of Gene Expression in the Murine Schwann Cell Lineage and the Acutely Injured Postnatal Nerve. PLoS ONE. 2016;11:e0153256 pubmed publisher
  30. Wu X, Fleming A, Ricketts T, Pavel M, Virgin H, Menzies F, et al. Autophagy regulates Notch degradation and modulates stem cell development and neurogenesis. Nat Commun. 2016;7:10533 pubmed publisher
  31. Gho C, Schomann T, de Groot S, Frijns J, Rivolta M, Neumann M, et al. Isolation, expansion and neural differentiation of stem cells from human plucked hair: a further step towards autologous nerve recovery. Cytotechnology. 2016;68:1849-58 pubmed publisher
  32. Krishnaswamy A, Yamagata M, Duan X, Hong Y, Sanes J. Sidekick 2 directs formation of a retinal circuit that detects differential motion. Nature. 2015;524:466-470 pubmed publisher
  33. Kim K, Ossipova O, Sokol S. Neural crest specification by inhibition of the ROCK/Myosin II pathway. Stem Cells. 2015;33:674-85 pubmed publisher
  34. Cyr A, Kulak M, Park J, Bogachek M, Spanheimer P, Woodfield G, et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene. 2015;34:436-44 pubmed publisher
  35. Kermorvant Duchemin E, Pinel A, Lavalette S, Lenne D, Raoul W, Calippe B, et al. Neonatal hyperglycemia inhibits angiogenesis and induces inflammation and neuronal degeneration in the retina. PLoS ONE. 2013;8:e79545 pubmed publisher
  36. Boije H, Ring H, Shirazi Fard S, Grundberg I, Nilsson M, Hallböök F. Alternative splicing of the chromodomain protein Morf4l1 pre-mRNA has implications on cell differentiation in the developing chicken retina. J Mol Neurosci. 2013;51:615-28 pubmed publisher
  37. Willaredt M, Gorgas K, Gardner H, Tucker K. Multiple essential roles for primary cilia in heart development. Cilia. 2012;1:23 pubmed publisher
  38. Danielson L, Park D, Rotllan N, Chamorro Jorganes A, Guijarro M, Fernandez Hernando C, et al. Cardiovascular dysregulation of miR-17-92 causes a lethal hypertrophic cardiomyopathy and arrhythmogenesis. FASEB J. 2013;27:1460-7 pubmed publisher
  39. Yan R, Liang L, Ma W, Li X, Xie W, Wang S. Neurogenin1 effectively reprograms cultured chick retinal pigment epithelial cells to differentiate toward photoreceptors. J Comp Neurol. 2010;518:526-46 pubmed publisher
  40. Fischer A, Stanke J, Aloisio G, Hoy H, Stell W. Heterogeneity of horizontal cells in the chicken retina. J Comp Neurol. 2007;500:1154-71 pubmed