This is a Validated Antibody Database (VAD) review about human AMPK, based on 19 published articles (read how Labome selects the articles), using AMPK antibody in all methods. It is aimed to help Labome visitors find the most suited AMPK antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
AMPK synonym: AMPK; HAMPKb

Knockout validation
Cell Signaling Technology
domestic rabbit monoclonal (57C12)
  • western blot; mouse; loading ...; fig 2a
  • western blot knockout validation; human; loading ...; fig s4a
Cell Signaling Technology AMPK antibody (Cell Signaling, 4150) was used in western blot on mouse samples (fig 2a) and in western blot knockout validation on human samples (fig s4a). J Biol Chem (2018) ncbi
Abcam
domestic rabbit monoclonal (Y367)
  • western blot; human; 1:2000; loading ...; fig 4d
Abcam AMPK antibody (Abcam, ab32112) was used in western blot on human samples at 1:2000 (fig 4d). Front Oncol (2021) ncbi
domestic rabbit monoclonal (Y367)
  • western blot; human; 1:1000; loading ...; fig 6c
Abcam AMPK antibody (Abcam, ab32112) was used in western blot on human samples at 1:1000 (fig 6c). EMBO Mol Med (2019) ncbi
domestic rabbit monoclonal (Y367)
  • western blot; mouse; loading ...; fig 5d
  • western blot; human; loading ...; fig 7d
Abcam AMPK antibody (Abcam, ab32112) was used in western blot on mouse samples (fig 5d) and in western blot on human samples (fig 7d). Hepatology (2018) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (57C12)
  • western blot; mouse; loading ...; fig 3a
Cell Signaling Technology AMPK antibody (Cell Signaling, 4150P) was used in western blot on mouse samples (fig 3a). Front Cell Dev Biol (2021) ncbi
domestic rabbit monoclonal (57C12)
  • western blot; mouse; 1:500; loading ...; fig 3c
Cell Signaling Technology AMPK antibody (CST, 4150) was used in western blot on mouse samples at 1:500 (fig 3c). Mol Brain (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:500; loading ...; fig 3c
Cell Signaling Technology AMPK antibody (CST, 4181) was used in western blot on mouse samples at 1:500 (fig 3c). Mol Brain (2021) ncbi
domestic rabbit monoclonal (57C12)
  • western blot; mouse; loading ...; fig 2a
  • western blot knockout validation; human; loading ...; fig s4a
Cell Signaling Technology AMPK antibody (Cell Signaling, 4150) was used in western blot on mouse samples (fig 2a) and in western blot knockout validation on human samples (fig s4a). J Biol Chem (2018) ncbi
domestic rabbit monoclonal (57C12)
  • western blot; human; 1:1000; loading ...; fig s2k
Cell Signaling Technology AMPK antibody (Cell Signaling, 4150) was used in western blot on human samples at 1:1000 (fig s2k). Nat Cell Biol (2018) ncbi
domestic rabbit monoclonal (57C12)
  • western blot; human; 1:1000; loading ...; fig 4a
In order to research the effect of methyl-beta-cyclodextrin on autophagy flux in Niemann-Pick C1-deficient cells, Cell Signaling Technology AMPK antibody (Cell Signaling, 4150s) was used in western blot on human samples at 1:1000 (fig 4a). Autophagy (2017) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; fig 7a
In order to study the function of OPA1 in FGF21 secretion from muscle, Cell Signaling Technology AMPK antibody (Cell signaling, 4181) was used in western blot on mouse samples at 1:1000 (fig 7a). EMBO J (2017) ncbi
domestic rabbit monoclonal (57C12)
  • western blot; mouse; loading ...; fig 1b
Cell Signaling Technology AMPK antibody (Cell Signaling, 4150) was used in western blot on mouse samples (fig 1b). Am J Physiol Endocrinol Metab (2016) ncbi
domestic rabbit monoclonal (57C12)
  • western blot; human; 1:1000; fig 2
Cell Signaling Technology AMPK antibody (Cell Signaling, 4150S) was used in western blot on human samples at 1:1000 (fig 2). Nat Cell Biol (2016) ncbi
domestic rabbit monoclonal (57C12)
  • western blot; mouse; 1:5000; fig 1b
In order to determine how reuglations of m TORC1 signalling occurs by SUMOylation of AMPK alpha 1 through PIAS4, Cell Signaling Technology AMPK antibody (Cell Signaling technology, 4150) was used in western blot on mouse samples at 1:5000 (fig 1b). Nat Commun (2015) ncbi
domestic rabbit monoclonal (71C10)
  • western blot; mouse; 1:1000; fig 7
Cell Signaling Technology AMPK antibody (Cell Signaling, 4178) was used in western blot on mouse samples at 1:1000 (fig 7). elife (2015) ncbi
domestic rabbit monoclonal (57C12)
  • immunocytochemistry; human; 1:400; fig 3
Cell Signaling Technology AMPK antibody (Cell Signaling, 4150) was used in immunocytochemistry on human samples at 1:400 (fig 3). Data Brief (2015) ncbi
domestic rabbit monoclonal (57C12)
  • western blot; human; fig 4a
Cell Signaling Technology AMPK antibody (Cell Signaling Technology;, 4150) was used in western blot on human samples (fig 4a). Autophagy (2015) ncbi
domestic rabbit monoclonal (57C12)
  • western blot; human; fig 2
Cell Signaling Technology AMPK antibody (Cell Signaling Tech, 4150) was used in western blot on human samples (fig 2). PLoS ONE (2015) ncbi
domestic rabbit monoclonal (57C12)
  • immunocytochemistry; human; 1:400; fig 3
Cell Signaling Technology AMPK antibody (Cell signaling, 4150) was used in immunocytochemistry on human samples at 1:400 (fig 3). Biochim Biophys Acta (2015) ncbi
domestic rabbit monoclonal (57C12)
  • western blot; human; fig 1a
  • western blot; mouse; fig 1b
Cell Signaling Technology AMPK antibody (Cell signalling, 4150) was used in western blot on human samples (fig 1a) and in western blot on mouse samples (fig 1b). Am J Cancer Res (2014) ncbi
domestic rabbit monoclonal (71C10)
  • western blot; pigs
Cell Signaling Technology AMPK antibody (Cell Signaling, 4178) was used in western blot on pigs samples . Basic Res Cardiol (2013) ncbi
Articles Reviewed
  1. Jiang D, Zhang J, Lin S, Wang Y, Chen Y, Fan J. Prolyl Endopeptidase Gene Disruption Improves Gut Dysbiosis and Non-alcoholic Fatty Liver Disease in Mice Induced by a High-Fat Diet. Front Cell Dev Biol. 2021;9:628143 pubmed publisher
  2. Yu F, Ma R, Liu C, Zhang L, Feng K, Wang M, et al. SQSTM1/p62 Promotes Cell Growth and Triggers Autophagy in Papillary Thyroid Cancer by Regulating the AKT/AMPK/mTOR Signaling Pathway. Front Oncol. 2021;11:638701 pubmed publisher
  3. Sowa A, Popova T, Harmuth T, Weber J, Pereira Sena P, Schmidt J, et al. Neurodegenerative phosphoprotein signaling landscape in models of SCA3. Mol Brain. 2021;14:57 pubmed publisher
  4. Li H, Yang F, Hu A, Wang X, Fang E, Chen Y, et al. Therapeutic targeting of circ-CUX1/EWSR1/MAZ axis inhibits glycolysis and neuroblastoma progression. EMBO Mol Med. 2019;11:e10835 pubmed publisher
  5. Wang Y, Du L, Liang X, Meng P, Bi L, Wang Y, et al. Sirtuin 4 Depletion Promotes Hepatocellular Carcinoma Tumorigenesis Through Regulating Adenosine-Monophosphate-Activated Protein Kinase Alpha/Mammalian Target of Rapamycin Axis in Mice. Hepatology. 2018;: pubmed publisher
  6. Hinchy E, Gruszczyk A, Willows R, Navaratnam N, Hall A, Bates G, et al. Mitochondria-derived ROS activate AMP-activated protein kinase (AMPK) indirectly. J Biol Chem. 2018;293:17208-17217 pubmed publisher
  7. Chhipa R, Fan Q, Anderson J, Muraleedharan R, Huang Y, Ciraolo G, et al. AMP kinase promotes glioblastoma bioenergetics and tumour growth. Nat Cell Biol. 2018;20:823-835 pubmed publisher
  8. Dai S, Dulcey A, Hu X, Wassif C, Porter F, Austin C, et al. Methyl-β-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK. Autophagy. 2017;13:1435-1451 pubmed publisher
  9. Pereira R, Tadinada S, Zasadny F, Oliveira K, Pires K, Olvera A, et al. OPA1 deficiency promotes secretion of FGF21 from muscle that prevents obesity and insulin resistance. EMBO J. 2017;36:2126-2145 pubmed publisher
  10. Bultot L, Jensen T, Lai Y, Madsen A, Collodet C, Kviklyte S, et al. Benzimidazole derivative small-molecule 991 enhances AMPK activity and glucose uptake induced by AICAR or contraction in skeletal muscle. Am J Physiol Endocrinol Metab. 2016;311:E706-E719 pubmed publisher
  11. Liu X, Xiao Z, Han L, Zhang J, Lee S, Wang W, et al. LncRNA NBR2 engages a metabolic checkpoint by regulating AMPK under energy stress. Nat Cell Biol. 2016;18:431-42 pubmed publisher
  12. Yan Y, Ollila S, Wong I, Vallenius T, Palvimo J, Vaahtomeri K, et al. SUMOylation of AMPKα1 by PIAS4 specifically regulates mTORC1 signalling. Nat Commun. 2015;6:8979 pubmed publisher
  13. Dijk W, Heine M, Vergnes L, Boon M, Schaart G, Hesselink M, et al. ANGPTL4 mediates shuttling of lipid fuel to brown adipose tissue during sustained cold exposure. elife. 2015;4: pubmed publisher
  14. Mahboubi H, Barisé R, Stochaj U. Data in support of 5'AMP-activated protein kinase alpha regulates stress granule biogenesis. Data Brief. 2015;4:54-9 pubmed publisher
  15. Drießen S, Berleth N, Friesen O, Löffler A, Böhler P, Hieke N, et al. Deubiquitinase inhibition by WP1130 leads to ULK1 aggregation and blockade of autophagy. Autophagy. 2015;11:1458-70 pubmed publisher
  16. Kemmerer M, Finkernagel F, Cavalcante M, Abdalla D, Müller R, Brüne B, et al. AMP-Activated Protein Kinase Interacts with the Peroxisome Proliferator-Activated Receptor Delta to Induce Genes Affecting Fatty Acid Oxidation in Human Macrophages. PLoS ONE. 2015;10:e0130893 pubmed publisher
  17. Mahboubi H, Barisé R, Stochaj U. 5'-AMP-activated protein kinase alpha regulates stress granule biogenesis. Biochim Biophys Acta. 2015;1853:1725-37 pubmed publisher
  18. Lau A, Liu P, Inuzuka H, Gao D. SIRT1 phosphorylation by AMP-activated protein kinase regulates p53 acetylation. Am J Cancer Res. 2014;4:245-55 pubmed
  19. Moberly S, Mather K, Berwick Z, Owen M, Goodwill A, Casalini E, et al. Impaired cardiometabolic responses to glucagon-like peptide 1 in obesity and type 2 diabetes mellitus. Basic Res Cardiol. 2013;108:365 pubmed publisher