This is a Validated Antibody Database (VAD) review about human AML1, based on 26 published articles (read how Labome selects the articles), using AML1 antibody in all methods. It is aimed to help Labome visitors find the most suited AML1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
AML1 synonym: AML1; AML1-EVI-1; AMLCR1; CBF2alpha; CBFA2; EVI-1; PEBP2aB; PEBP2alpha

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (DW71)
  • immunohistochemistry knockout validation; mouse; loading ...; fig 2f
Santa Cruz Biotechnology AML1 antibody (Santa Cruz, sc-101146) was used in immunohistochemistry knockout validation on mouse samples (fig 2f). PLoS Genet (2021) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; loading ...; fig 1c
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2a
Abcam AML1 antibody (Abcam, 23980) was used in immunohistochemistry knockout validation on mouse samples (fig 1c) and in immunohistochemistry - paraffin section on mouse samples (fig 2a). Sci Rep (2019) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 3b
Cell Signaling Technology AML1 antibody (Cell Signaling, 4334s) was used in western blot knockout validation on human samples at 1:1000 (fig 3b). PLoS Genet (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D4A6)
  • western blot knockout validation; mouse; loading ...; fig 3c, 7d
Cell Signaling Technology AML1 antibody (Cell signaling, 8529s) was used in western blot knockout validation on mouse samples (fig 3c, 7d). PLoS Genet (2021) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 1d
Cell Signaling Technology AML1 antibody (CST, 4334s) was used in western blot knockout validation on human samples at 1:1000 (fig 1d). Nat Commun (2019) ncbi
Santa Cruz Biotechnology
mouse monoclonal (DW71)
  • immunohistochemistry knockout validation; mouse; loading ...; fig 2f
Santa Cruz Biotechnology AML1 antibody (Santa Cruz, sc-101146) was used in immunohistochemistry knockout validation on mouse samples (fig 2f). PLoS Genet (2021) ncbi
mouse monoclonal (A-2)
  • immunocytochemistry; zebrafish ; loading ...; fig s9f
Santa Cruz Biotechnology AML1 antibody (Santa, sc-365644) was used in immunocytochemistry on zebrafish samples (fig s9f). Science (2019) ncbi
mouse monoclonal (A-2)
  • western blot; human; loading ...; fig 3c
Santa Cruz Biotechnology AML1 antibody (Santa Cruz, sc- 365644) was used in western blot on human samples (fig 3c). Blood Adv (2018) ncbi
mouse monoclonal (A-2)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2e
In order to utilize a G2-Gata4Cre;R26REYFP mouse line to track the developmental fate of the G2-Gata4 cell lineage, Santa Cruz Biotechnology AML1 antibody (Santa Cruz Biotechnology, Sc-365644) was used in immunohistochemistry - frozen section on mouse samples (fig 2e). Haematologica (2017) ncbi
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Santa Cruz Biotechnology AML1 antibody (SCBT, H-65) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
  • western blot; mouse; 1:100
Santa Cruz Biotechnology AML1 antibody (Santa Cruz, sc28679) was used in western blot on mouse samples at 1:100. Sci Rep (2016) ncbi
mouse monoclonal (A-2)
  • western blot; mouse
Santa Cruz Biotechnology AML1 antibody (Santa Cruz, sc-365644) was used in western blot on mouse samples . J Biol Chem (2015) ncbi
mouse monoclonal (DW71)
  • western blot; mouse
In order to study the role of Runx1 and PU.1 in corepressor-coactivator exchange and the potential role of PU.1-recruited corepressors as targets for leukemia differentiation therapy, Santa Cruz Biotechnology AML1 antibody (Santa Cruz Biotechnology, sc-101146) was used in western blot on mouse samples . J Biol Chem (2014) ncbi
Abcam
domestic rabbit monoclonal (EPR23044-100)
  • immunohistochemistry; human; 1:2000; loading ...; fig s3e
Abcam AML1 antibody (abcam, ab240639) was used in immunohistochemistry on human samples at 1:2000 (fig s3e). Genome Biol (2021) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; human; ; loading ...; fig 2c
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 6c
Abcam AML1 antibody (Abcam, ab23980) was used in ChIP-Seq on human samples at (fig 2c) and in immunohistochemistry - paraffin section on human samples at 1:100 (fig 6c). PLoS Genet (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; loading ...; fig 1c
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2a
Abcam AML1 antibody (Abcam, 23980) was used in immunohistochemistry knockout validation on mouse samples (fig 1c) and in immunohistochemistry - paraffin section on mouse samples (fig 2a). Sci Rep (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 7f
  • chromatin immunoprecipitation; human; loading ...; fig 7d
  • immunocytochemistry; human; 1:100; loading ...; fig 1g
Abcam AML1 antibody (Abcam, ab23980) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 7f), in chromatin immunoprecipitation on human samples (fig 7d) and in immunocytochemistry on human samples at 1:100 (fig 1g). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; human; fig 4b
Abcam AML1 antibody (Abcam, ab23980) was used in ChIP-Seq on human samples (fig 4b). Cell (2018) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig s5b
Abcam AML1 antibody (Abcam, ab23980) was used in ChIP-Seq on mouse samples (fig s5b). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • ChIP-Seq; mouse; loading ...; fig 5b
Abcam AML1 antibody (Abcam, ab23980) was used in ChIP-Seq on mouse samples (fig 5b). Leukemia (2018) ncbi
domestic rabbit polyclonal
  • chromatin immunoprecipitation; mouse; loading ...; fig 1a
In order to study the role of RUNX1 and FOXP3 interplay in expression of breast cancer related genes, Abcam AML1 antibody (Abcam, 23980) was used in chromatin immunoprecipitation on mouse samples (fig 1a). Oncotarget (2016) ncbi
Invitrogen
rat monoclonal (RXDMC)
  • flow cytometry; mouse; loading ...; fig 4c
Invitrogen AML1 antibody (eBioscience, RXDMC) was used in flow cytometry on mouse samples (fig 4c). J Exp Med (2018) ncbi
rat monoclonal (RXDMC)
Invitrogen AML1 antibody (eBioscience, 12-9816-80) was used . Nat Commun (2017) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 3b
Cell Signaling Technology AML1 antibody (Cell Signaling, 4334s) was used in western blot knockout validation on human samples at 1:1000 (fig 3b). PLoS Genet (2021) ncbi
domestic rabbit monoclonal (D4A6)
  • western blot knockout validation; mouse; loading ...; fig 3c, 7d
Cell Signaling Technology AML1 antibody (Cell signaling, 8529s) was used in western blot knockout validation on mouse samples (fig 3c, 7d). PLoS Genet (2021) ncbi
domestic rabbit monoclonal (D4A6)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 1a
Cell Signaling Technology AML1 antibody (Cell Signaling, 8529) was used in immunohistochemistry on mouse samples at 1:100 (fig 1a). elife (2020) ncbi
domestic rabbit monoclonal (D33G6)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1d
Cell Signaling Technology AML1 antibody (Cell Signaling, D33G6) was used in immunohistochemistry on mouse samples at 1:200 (fig 1d). Sci Rep (2020) ncbi
domestic rabbit monoclonal (D33G6)
  • immunohistochemistry; human; loading ...; fig 1g
Cell Signaling Technology AML1 antibody (CST, 4336) was used in immunohistochemistry on human samples (fig 1g). J Exp Clin Cancer Res (2019) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; human; 1:1000; loading ...; fig 1d
Cell Signaling Technology AML1 antibody (CST, 4334s) was used in western blot knockout validation on human samples at 1:1000 (fig 1d). Nat Commun (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig s2
In order to report the O-linked N-acetylglucosamine glycoproteome observed in human T cells, Cell Signaling Technology AML1 antibody (Cell Signaling, 4334) was used in western blot on human samples (fig s2). J Immunol (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; fig 1
In order to characterize a novel signaling pathway in thyroid cancer that mediates runt-related transcription factor 2 (Runx2) expression in thyroid cancer cells by thyroid hormone receptor beta (TRbeta), Cell Signaling Technology AML1 antibody (Cell signaling, CS4334) was used in immunohistochemistry - paraffin section on human samples (fig 1). Endocrinology (2016) ncbi
domestic rabbit monoclonal (D33G6)
  • western blot; human; 1:1000; loading ...; fig 2B
Cell Signaling Technology AML1 antibody (Cell Signaling, 4336) was used in western blot on human samples at 1:1000 (fig 2B). Mol Med Rep (2015) ncbi
domestic rabbit monoclonal (D4A6)
  • immunocytochemistry; human
  • western blot; human
In order to identify DUBA as a suppressor of IL-17 production in T cells, Cell Signaling Technology AML1 antibody (Cell Signaling, D4A6) was used in immunocytochemistry on human samples and in western blot on human samples . Nature (2015) ncbi
domestic rabbit monoclonal (D33G6)
  • immunoprecipitation; human; fig 6
  • western blot; human; fig 6
In order to study the effect of panobinostat treatment on disease using an acute myeloid leukemia mouse model, Cell Signaling Technology AML1 antibody (Cell Signaling Technology, 4336) was used in immunoprecipitation on human samples (fig 6) and in western blot on human samples (fig 6). Blood (2014) ncbi
Leica Biosystems
  • immunohistochemistry - paraffin section; human; 1:15; fig 1c
Leica Biosystems AML1 antibody (Leica Biosystems, NCL-L-MITF) was used in immunohistochemistry - paraffin section on human samples at 1:15 (fig 1c). elife (2016) ncbi
Articles Reviewed
  1. Yuan C, Chen H, Tu S, Huang H, Pan Y, Gui X, et al. A systematic dissection of the epigenomic heterogeneity of lung adenocarcinoma reveals two different subclasses with distinct prognosis and core regulatory networks. Genome Biol. 2021;22:156 pubmed publisher
  2. Malik N, Yan H, Yang H, Ayaz G, DuBois W, Tseng Y, et al. CBFB cooperates with p53 to maintain TAp73 expression and suppress breast cancer. PLoS Genet. 2021;17:e1009553 pubmed publisher
  3. Tang C, Wu M, Zhao D, Edwards D, McVicar A, Luo Y, et al. Runx1 is a central regulator of osteogenesis for bone homeostasis by orchestrating BMP and WNT signaling pathways. PLoS Genet. 2021;17:e1009233 pubmed publisher
  4. Mevel R, Steiner I, Mason S, Galbraith L, Patel R, Fadlullah M, et al. RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development. elife. 2020;9: pubmed publisher
  5. Lee Thacker S, Jeon H, Choi Y, Taniuchi I, Takarada T, Yoneda Y, et al. Core Binding Factors are essential for ovulation, luteinization, and female fertility in mice. Sci Rep. 2020;10:9921 pubmed publisher
  6. Li Q, Lai Q, He C, Fang Y, Yan Q, Zhang Y, et al. RUNX1 promotes tumour metastasis by activating the Wnt/β-catenin signalling pathway and EMT in colorectal cancer. J Exp Clin Cancer Res. 2019;38:334 pubmed publisher
  7. Yano F, Ohba S, Murahashi Y, Tanaka S, Saito T, Chung U. Runx1 contributes to articular cartilage maintenance by enhancement of cartilage matrix production and suppression of hypertrophic differentiation. Sci Rep. 2019;9:7666 pubmed publisher
  8. Malik N, Yan H, Moshkovich N, Palangat M, Yang H, Sanchez V, et al. The transcription factor CBFB suppresses breast cancer through orchestrating translation and transcription. Nat Commun. 2019;10:2071 pubmed publisher
  9. Gu Q, Yang X, Lv J, Zhang J, Xia B, Kim J, et al. AIBP-mediated cholesterol efflux instructs hematopoietic stem and progenitor cell fate. Science. 2019;363:1085-1088 pubmed publisher
  10. Pulikkan J, Hegde M, Ahmad H, Belaghzal H, Illendula A, Yu J, et al. CBFβ-SMMHC Inhibition Triggers Apoptosis by Disrupting MYC Chromatin Dynamics in Acute Myeloid Leukemia. Cell. 2018;174:172-186.e21 pubmed publisher
  11. Jalagadugula G, Goldfinger L, Mao G, Lambert M, Rao A. Defective RAB1B-related megakaryocytic ER-to-Golgi transport in RUNX1 haplodeficiency: impact on von Willebrand factor. Blood Adv. 2018;2:797-806 pubmed publisher
  12. Harly C, Cam M, Kaye J, Bhandoola A. Development and differentiation of early innate lymphoid progenitors. J Exp Med. 2018;215:249-262 pubmed publisher
  13. Mao A, Ishizuka I, Kasal D, Mandal M, Bendelac A. A shared Runx1-bound Zbtb16 enhancer directs innate and innate-like lymphoid lineage development. Nat Commun. 2017;8:863 pubmed publisher
  14. Mingay M, Chaturvedi A, Bilenky M, Cao Q, Jackson L, Hui T, et al. Vitamin C-induced epigenomic remodelling in IDH1 mutant acute myeloid leukaemia. Leukemia. 2018;32:11-20 pubmed publisher
  15. Cañete A, Carmona R, Ariza L, Sanchez M, Rojas A, Muñoz Chápuli R. A population of hematopoietic stem cells derives from GATA4-expressing progenitors located in the placenta and lateral mesoderm of mice. Haematologica. 2017;102:647-655 pubmed publisher
  16. Lund P, Elias J, Davis M. Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol. 2016;197:3086-3098 pubmed
  17. CARR F, Tai P, Barnum M, Gillis N, Evans K, Taber T, et al. Thyroid Hormone Receptor-? (TR?) Mediates Runt-Related Transcription Factor 2 (Runx2) Expression in Thyroid Cancer Cells: A Novel Signaling Pathway in Thyroid Cancer. Endocrinology. 2016;157:3278-92 pubmed publisher
  18. Natale C, Duperret E, Zhang J, Sadeghi R, Dahal A, O Brien K, et al. Sex steroids regulate skin pigmentation through nonclassical membrane-bound receptors. elife. 2016;5: pubmed publisher
  19. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  20. Recouvreux M, Grasso E, Echeverria P, Rocha Viegas L, Castilla L, Schere Levy C, et al. RUNX1 and FOXP3 interplay regulates expression of breast cancer related genes. Oncotarget. 2016;7:6552-65 pubmed publisher
  21. Aini H, Itaka K, Fujisawa A, Uchida H, Uchida S, Fukushima S, et al. Messenger RNA delivery of a cartilage-anabolic transcription factor as a disease-modifying strategy for osteoarthritis treatment. Sci Rep. 2016;6:18743 pubmed publisher
  22. Majumder A, Syed K, Joseph S, Scambler P, Dutta D. Histone Chaperone HIRA in Regulation of Transcription Factor RUNX1. J Biol Chem. 2015;290:13053-63 pubmed publisher
  23. Xia J, Chen S, Lv Y, Lu L, Hu W, Zhou Y. ZGDHu-1 induces Gâ‚‚/M phase arrest and apoptosis in Kasumi-1 cells. Mol Med Rep. 2015;11:3398-404 pubmed publisher
  24. Rutz S, Kayagaki N, Phung Q, Eidenschenk C, Noubade R, Wang X, et al. Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells. Nature. 2015;518:417-21 pubmed publisher
  25. Gu X, Hu Z, Ebrahem Q, Crabb J, Mahfouz R, Radivoyevitch T, et al. Runx1 regulation of Pu.1 corepressor/coactivator exchange identifies specific molecular targets for leukemia differentiation therapy. J Biol Chem. 2014;289:14881-95 pubmed publisher
  26. Bots M, Verbrugge I, Martin B, Salmon J, Ghisi M, Baker A, et al. Differentiation therapy for the treatment of t(8;21) acute myeloid leukemia using histone deacetylase inhibitors. Blood. 2014;123:1341-52 pubmed publisher