This is a Validated Antibody Database (VAD) review about human AMFR, based on 7 published articles (read how Labome selects the articles), using AMFR antibody in all methods. It is aimed to help Labome visitors find the most suited AMFR antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
AMFR synonym: GP78; RNF45; E3 ubiquitin-protein ligase AMFR; RING finger protein 45; RING-type E3 ubiquitin transferase AMFR

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (F-3)
  • western blot knockout validation; human; loading ...; fig 7a
Santa Cruz Biotechnology AMFR antibody (Santa Cruz, sc-166358) was used in western blot knockout validation on human samples (fig 7a). J Biol Chem (2019) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig 2b
Cell Signaling Technology AMFR antibody (Cell Signaling, 9590) was used in western blot knockout validation on human samples (fig 2b). Sci Rep (2018) ncbi
Santa Cruz Biotechnology
mouse monoclonal (F-3)
  • western blot knockout validation; human; loading ...; fig 7a
Santa Cruz Biotechnology AMFR antibody (Santa Cruz, sc-166358) was used in western blot knockout validation on human samples (fig 7a). J Biol Chem (2019) ncbi
mouse monoclonal (F-3)
  • western blot; human; fig 4
Santa Cruz Biotechnology AMFR antibody (Santa Cruz, SC-166358) was used in western blot on human samples (fig 4). J Biol Chem (2016) ncbi
mouse monoclonal (F-3)
  • western blot; mouse; 1:250; fig 2
  • western blot; human; 1:250; fig 2
In order to determine how mitochondrial homeostasis is affected by ubiquitin-mediated regulation of E3 ligase GP78 by MGRN1 in trans, Santa Cruz Biotechnology AMFR antibody (Santa Cruz, sc-166358) was used in western blot on mouse samples at 1:250 (fig 2) and in western blot on human samples at 1:250 (fig 2). J Cell Sci (2016) ncbi
Proteintech Group
rabbit polyclonal
  • western blot; mouse; loading ...; fig s5e
Proteintech Group AMFR antibody (Proteintech, 16675-1-AP) was used in western blot on mouse samples (fig s5e). Cell (2016) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig 2b
Cell Signaling Technology AMFR antibody (Cell Signaling, 9590) was used in western blot knockout validation on human samples (fig 2b). Sci Rep (2018) ncbi
rabbit polyclonal
  • immunoprecipitation; human; loading ...; fig 2g
  • western blot; human; loading ...; fig 2e
Cell Signaling Technology AMFR antibody (Cell Signaling, 9590) was used in immunoprecipitation on human samples (fig 2g) and in western blot on human samples (fig 2e). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; fig s1
Cell Signaling Technology AMFR antibody (Cell Signaling Technology, 9590S) was used in western blot on human samples (fig s1). Am J Transl Res (2015) ncbi
Articles Reviewed
  1. Zhang Y, Lu J, Ma J, Liu X. Insulin-induced gene 1 (INSIG1) inhibits HIV-1 production by degrading Gag via activity of the ubiquitin ligase TRC8. J Biol Chem. 2019;294:2046-2059 pubmed publisher
  2. Kadowaki H, Satrimafitrah P, Takami Y, Nishitoh H. Molecular mechanism of ER stress-induced pre-emptive quality control involving association of the translocon, Derlin-1, and HRD1. Sci Rep. 2018;8:7317 pubmed publisher
  3. Li J, Huang W, Lin P, Wu B, Fu Z, Shen H, et al. N-linked glycosylation at Asn152 on CD147 affects protein folding and stability: promoting tumour metastasis in hepatocellular carcinoma. Sci Rep. 2016;6:35210 pubmed publisher
  4. Llambi F, Wang Y, Victor B, Yang M, Schneider D, Gingras S, et al. BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation. Cell. 2016;165:421-33 pubmed publisher
  5. Di X, Wang Y, Han D, Fu Y, Duerfeldt A, Blagg B, et al. Grp94 Protein Delivers γ-Aminobutyric Acid Type A (GABAA) Receptors to Hrd1 Protein-mediated Endoplasmic Reticulum-associated Degradation. J Biol Chem. 2016;291:9526-39 pubmed publisher
  6. Mukherjee R, Chakrabarti O. Ubiquitin-mediated regulation of the E3 ligase GP78 by MGRN1 in trans affects mitochondrial homeostasis. J Cell Sci. 2016;129:757-73 pubmed publisher
  7. Sun Y, Ponz Sarvisé M, Chang S, Chang W, Chen C, Hsu J, et al. Proteasome inhibition enhances the killing effect of BikDD gene therapy. Am J Transl Res. 2015;7:319-27 pubmed