This is a Validated Antibody Database (VAD) review about human AMBRA1, based on 4 published articles (read how Labome selects the articles), using AMBRA1 antibody in all methods. It is aimed to help Labome visitors find the most suited AMBRA1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
AMBRA1 synonym: DCAF3; WDR94; activating molecule in BECN1-regulated autophagy protein 1; DDB1 and CUL4 associated factor 3; WD repeat domain 94; activating molecule in beclin-1-regulated autophagy; autophagy/beclin-1 regulator 1

Santa Cruz Biotechnology
mouse monoclonal
  • western blot; human; loading ...; fig 5a
In order to research the role of MIR7-3HG in autophagy regulation, Santa Cruz Biotechnology AMBRA1 antibody (Santa Cruz, sc-398204) was used in western blot on human samples (fig 5a). Autophagy (2017) ncbi
mouse monoclonal
  • immunoprecipitation; human; fig 4
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 1
Santa Cruz Biotechnology AMBRA1 antibody (Santa Cruz, sc-398204) was used in immunoprecipitation on human samples (fig 4), in immunocytochemistry on human samples (fig 1) and in western blot on human samples (fig 1). Autophagy (2016) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot; mouse; 1:1000; fig s2
In order to identify the role of defective autophagy in cerebral cavernous malformations, Sigma-Aldrich AMBRA1 antibody (Sigma-Aldrich, PRS4555) was used in western blot on mouse samples at 1:1000 (fig s2). EMBO Mol Med (2015) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; mouse; 1:1000; fig s2
In order to identify the role of defective autophagy in cerebral cavernous malformations, EMD Millipore AMBRA1 antibody (Millipore, ABC80) was used in western blot on mouse samples at 1:1000 (fig s2). EMBO Mol Med (2015) ncbi
ProSci
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100
ProSci AMBRA1 antibody (ProSci Incorporated, 4557) was used in immunohistochemistry - paraffin section on mouse samples at 1:100. Neurobiol Aging (2014) ncbi
Articles Reviewed
  1. Capizzi M, Strappazzon F, Cianfanelli V, Papaleo E, Cecconi F. MIR7-3HG, a MYC-dependent modulator of cell proliferation, inhibits autophagy by a regulatory loop involving AMBRA1. Autophagy. 2017;13:554-566 pubmed publisher
  2. Strappazzon F, Di Rita A, Cianfanelli V, D Orazio M, Nazio F, Fimia G, et al. Prosurvival AMBRA1 turns into a proapoptotic BH3-like protein during mitochondrial apoptosis. Autophagy. 2016;12:963-75 pubmed publisher
  3. Marchi S, Corricelli M, Trapani E, Bravi L, Pittaro A, Delle Monache S, et al. Defective autophagy is a key feature of cerebral cavernous malformations. EMBO Mol Med. 2015;7:1403-17 pubmed publisher
  4. Sepe S, Nardacci R, Fanelli F, Rosso P, Bernardi C, Cecconi F, et al. Expression of Ambra1 in mouse brain during physiological and Alzheimer type aging. Neurobiol Aging. 2014;35:96-108 pubmed publisher