This is a Validated Antibody Database (VAD) review about human ALK, based on 29 published articles (read how Labome selects the articles), using ALK antibody in all methods. It is aimed to help Labome visitors find the most suited ALK antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ALK synonym: CD246; NBLST3

Invitrogen
domestic rabbit monoclonal (SP8)
  • ELISA; human; loading ...; fig s3
Invitrogen ALK antibody (Thermo, MA5-14528) was used in ELISA on human samples (fig s3). Sci Rep (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 1
In order to study ALK oncogenes in conventional spindle-cell and typical inflammatory myofibroblastic tumors, Invitrogen ALK antibody (Invitrogen, 51-3900) was used in western blot on human samples (fig 1). J Pathol (2017) ncbi
mouse monoclonal (4C5B8)
  • western blot; human; fig 4
In order to characterize multiple molecularly defined cancer indications by studying ROS1, ALK inhibitor, Entrectinib, a Pan-TRK activity, Invitrogen ALK antibody (Invitrogen, 35-4300) was used in western blot on human samples (fig 4). Mol Cancer Ther (2016) ncbi
mouse monoclonal (4C5B8)
  • immunocytochemistry; human; fig 1
  • immunohistochemistry; human; fig 1
  • western blot; human; fig 1
In order to study NPM-ALK-amplified cell lines resistant to ALK tyrosine kinase inhibitors, Invitrogen ALK antibody (Invitrogen, 35-4300) was used in immunocytochemistry on human samples (fig 1), in immunohistochemistry on human samples (fig 1) and in western blot on human samples (fig 1). Oncogene (2016) ncbi
mouse monoclonal (5A4)
  • immunohistochemistry - paraffin section; human
In order to describe the clinicopathologically features of two cases of kidney mucinous tubular and spindle cell carcinoma, Invitrogen ALK antibody (THERMO, 5A4) was used in immunohistochemistry - paraffin section on human samples . Int J Clin Exp Pathol (2015) ncbi
mouse monoclonal (4C5B8)
  • western blot; human; fig 1
In order to identify and characterize an anaplastic lymphoma kinase inhibitor, Invitrogen ALK antibody (Invitrogen, 35-4300) was used in western blot on human samples (fig 1). Mol Cancer Ther (2012) ncbi
mouse monoclonal (5A4)
  • immunohistochemistry - paraffin section; human
  • immunohistochemistry; human
In order to investigate the clinical and pathological features in a girl with common variable immunodeficiency, Invitrogen ALK antibody (Thermo, 5A4) was used in immunohistochemistry - paraffin section on human samples and in immunohistochemistry on human samples . Int J Surg Pathol (2014) ncbi
mouse monoclonal (4C5B8)
  • immunocytochemistry; human
  • western blot; human
  • immunocytochemistry; mouse
In order to examine the consequences of constitutive expression of anaplastic lymphoma kinase, Invitrogen ALK antibody (Zymed, 4C5B8) was used in immunocytochemistry on human samples , in western blot on human samples and in immunocytochemistry on mouse samples . Blood (2006) ncbi
Santa Cruz Biotechnology
mouse monoclonal
  • western blot; mouse; loading ...; fig 3a
Santa Cruz Biotechnology ALK antibody (Santa Cruz Biotechnology, Inc, sc-398791) was used in western blot on mouse samples (fig 3a). Int J Oncol (2018) ncbi
mouse monoclonal
  • western blot; human; 1:100; loading ...; fig 8c
Santa Cruz Biotechnology ALK antibody (Santa Cruz Biotechnology Inc, c-398791) was used in western blot on human samples at 1:100 (fig 8c). Sci Transl Med (2017) ncbi
Abcam
domestic rabbit monoclonal (SP8)
  • western blot; mouse; fig 7
In order to analyze promotion of development of distinct sarcoma subtypes in hepatocyte growth factor-mediated satellite cells niche disruption, Abcam ALK antibody (Abcam, ab16670) was used in western blot on mouse samples (fig 7). elife (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C26G7)
  • western blot; human; loading ...; fig e3c
Cell Signaling Technology ALK antibody (CST, C26G7) was used in western blot on human samples (fig e3c). Nature (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2e
Cell Signaling Technology ALK antibody (Cell Signaling, 3983) was used in western blot on human samples (fig 2e). Oncotarget (2018) ncbi
mouse monoclonal (31F12)
  • western blot; human; loading ...; fig 2e
Cell Signaling Technology ALK antibody (Cell Signaling, 3791) was used in western blot on human samples (fig 2e). Oncotarget (2018) ncbi
domestic rabbit monoclonal (C26G7)
  • western blot; human; loading ...; fig 3c
Cell Signaling Technology ALK antibody (Cell Signaling, C26G7) was used in western blot on human samples (fig 3c). Proc Natl Acad Sci U S A (2017) ncbi
domestic rabbit monoclonal (C26G7)
  • western blot; human; fig 1b
Cell Signaling Technology ALK antibody (Cell Signaling, 3333) was used in western blot on human samples (fig 1b). Cancer Res (2017) ncbi
domestic rabbit monoclonal (D5F3)
  • proximity ligation assay; human; fig s7l
Cell Signaling Technology ALK antibody (Cell Signaling, D5F3) was used in proximity ligation assay on human samples (fig s7l). Cancer Res (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 1b
Cell Signaling Technology ALK antibody (Cell Signaling, 3983) was used in western blot on human samples (fig 1b). Cancer Res (2017) ncbi
domestic rabbit monoclonal (D5F3)
  • western blot; human; 1:1000; loading ...; fig 2a
Cell Signaling Technology ALK antibody (cell signalling, D5F3) was used in western blot on human samples at 1:1000 (fig 2a). Cancer Sci (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; fig s7
In order to analyze promotion of development of distinct sarcoma subtypes in hepatocyte growth factor-mediated satellite cells niche disruption, Cell Signaling Technology ALK antibody (Cell signaling, 3341) was used in western blot on human samples (fig s7). elife (2016) ncbi
domestic rabbit monoclonal (D5F3)
  • western blot; human; fig s7
In order to analyze promotion of development of distinct sarcoma subtypes in hepatocyte growth factor-mediated satellite cells niche disruption, Cell Signaling Technology ALK antibody (Cell signaling, 3633) was used in western blot on human samples (fig s7). elife (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 4
In order to characterize multiple molecularly defined cancer indications by studying ROS1, ALK inhibitor, Entrectinib, a Pan-TRK activity, Cell Signaling Technology ALK antibody (Cell signaling, 3341) was used in western blot on human samples (fig 4). Mol Cancer Ther (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; fig 1
  • immunohistochemistry; human; fig 1
  • western blot; human; fig 1
In order to study NPM-ALK-amplified cell lines resistant to ALK tyrosine kinase inhibitors, Cell Signaling Technology ALK antibody (Cell Signaling Technology, 3341) was used in immunocytochemistry on human samples (fig 1), in immunohistochemistry on human samples (fig 1) and in western blot on human samples (fig 1). Oncogene (2016) ncbi
domestic rabbit monoclonal (C26G7)
  • western blot; human; fig 1
Cell Signaling Technology ALK antibody (Cell Signaling Technology, 3333) was used in western blot on human samples (fig 1). Oncogene (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 1
Cell Signaling Technology ALK antibody (Cell Signaling Technology, 3341) was used in western blot on human samples (fig 1). Oncogene (2016) ncbi
domestic rabbit monoclonal (C26G7)
  • western blot; human; 1:1000
In order to discuss the sensitivity and selectivity of seven ROS1 and/or ALK inhibitors, Cell Signaling Technology ALK antibody (CST, 3333) was used in western blot on human samples at 1:1000. Proc Natl Acad Sci U S A (2015) ncbi
domestic rabbit monoclonal (D59G10)
  • western blot; human; 1:1000
In order to discuss the sensitivity and selectivity of seven ROS1 and/or ALK inhibitors, Cell Signaling Technology ALK antibody (CST, 6941) was used in western blot on human samples at 1:1000. Proc Natl Acad Sci U S A (2015) ncbi
domestic rabbit monoclonal (D59G10)
  • western blot; human
In order to test if midkine and anaplastic lymphoma kinase expression and signaling are activated by ethanol, Cell Signaling Technology ALK antibody (Cell Signaling Technology, 6941) was used in western blot on human samples . J Neurochem (2015) ncbi
domestic rabbit monoclonal (C26G7)
  • western blot; human; fig 6
Cell Signaling Technology ALK antibody (Cell Signaling, 3333) was used in western blot on human samples (fig 6). Cell Res (2015) ncbi
domestic rabbit monoclonal (C26G7)
  • western blot; human; 1:1000
  • western blot; mouse; 1:1000
Cell Signaling Technology ALK antibody (Cell Signaling, 3333) was used in western blot on human samples at 1:1000 and in western blot on mouse samples at 1:1000. Cancer Res (2013) ncbi
Dako
mouse monoclonal (ALK1)
  • immunoprecipitation; human; fig 1
In order to study ALK oncogenes in conventional spindle-cell and typical inflammatory myofibroblastic tumors, Dako ALK antibody (Dako, ALK1) was used in immunoprecipitation on human samples (fig 1). J Pathol (2017) ncbi
mouse monoclonal (ALK1)
  • western blot; human; fig 6
In order to analyze NPM-ALK+ T-cell lymphoma and the transcription factors MZF1 and Ik-1 that downregulate IGF-IR expression, Dako ALK antibody (Dako, M719501-2) was used in western blot on human samples (fig 6). Mol Cancer (2015) ncbi
mouse monoclonal (ALK1)
  • immunohistochemistry - paraffin section; human; ready-to-use
In order to determine if the presence of the DUSP22-IRF4 translocation is a distinctive histological features in patients with cutaneous anaplastic large cell lymphoma, Dako ALK antibody (Dako, ALK-1) was used in immunohistochemistry - paraffin section on human samples at ready-to-use. Histopathology (2015) ncbi
mouse monoclonal (ALK1)
  • immunohistochemistry - paraffin section; mouse; 1:50
In order to determine the effects of ASP3026treatment on NPM-ALK+ ALCL cell lines in vitro and on systemic lymphoma growth in vivo, Dako ALK antibody (Dako, M719501-2) was used in immunohistochemistry - paraffin section on mouse samples at 1:50. Oncotarget (2014) ncbi
mouse monoclonal (ALK1)
  • immunohistochemistry - paraffin section; human; 1:100
In order to report on a case of CD30-positive extranodal natural killer/T-cell lymphoma, Dako ALK antibody (Dako, ALK1) was used in immunohistochemistry - paraffin section on human samples at 1:100. Oncol Lett (2014) ncbi
mouse monoclonal (ALK1)
  • blocking or activating experiments; human
Dako ALK antibody (Dako, M7195) was used in blocking or activating experiments on human samples . Int J Oncol (2014) ncbi
Leica Biosystems
  • immunohistochemistry - paraffin section; human; 1:50; fig 1G
Leica Biosystems ALK antibody (Novocastra/Leica, NCL-L-ALK) was used in immunohistochemistry - paraffin section on human samples at 1:50 (fig 1G). Oncol Lett (2017) ncbi
monoclonal (5A4)
  • immunohistochemistry - paraffin section; human; 1:50
In order to examine the effects of imatinib on T and B cells in patients with chronic myelogenous leukemia, Leica Biosystems ALK antibody (Leica Biosystems, PA0308) was used in immunohistochemistry - paraffin section on human samples at 1:50. Oncol Lett (2016) ncbi
BD Biosciences
  • flow cytometry; human; fig st1
In order to find cell-surface markers specific to human neutrophils, BD Biosciences ALK antibody (BD, 559257) was used in flow cytometry on human samples (fig st1). Exp Cell Res (2016) ncbi
Articles Reviewed
  1. Kourentzi K, Crum M, Patil U, Prebisch A, Chavan D, Vu B, et al. Recombinant expression, characterization, and quantification in human cancer cell lines of the Anaplastic Large-Cell Lymphoma-characteristic NPM-ALK fusion protein. Sci Rep. 2020;10:5078 pubmed publisher
  2. Garcia Bermudez J, Baudrier L, Bayraktar E, Shen Y, La K, Guarecuco R, et al. Squalene accumulation in cholesterol auxotrophic lymphomas prevents oxidative cell death. Nature. 2019;567:118-122 pubmed publisher
  3. Liu S, Wang F, Liu G. Knockdown of pleiotrophin increases the risk of preeclampsia following vitrified-thawed embryo transfer. Int J Oncol. 2018;53:1847-1856 pubmed publisher
  4. Pilling A, Kim J, Estrada Bernal A, Zhou Q, Le A, Singleton K, et al. ALK is a critical regulator of the MYC-signaling axis in ALK positive lung cancer. Oncotarget. 2018;9:8823-8835 pubmed publisher
  5. Zeng L, Kang R, Zhu S, Wang X, Cao L, Wang H, et al. ALK is a therapeutic target for lethal sepsis. Sci Transl Med. 2017;9: pubmed publisher
  6. Rolland D, Basrur V, Jeon Y, McNeil Schwalm C, Fermin D, Conlon K, et al. Functional proteogenomics reveals biomarkers and therapeutic targets in lymphomas. Proc Natl Acad Sci U S A. 2017;114:6581-6586 pubmed publisher
  7. Vaishnavi A, Schubert L, Rix U, Marek L, Le A, Keysar S, et al. EGFR Mediates Responses to Small-Molecule Drugs Targeting Oncogenic Fusion Kinases. Cancer Res. 2017;77:3551-3563 pubmed publisher
  8. Wang R, Deng X, Yoshioka Y, Vougiouklakis T, Park J, Suzuki T, et al. Effects of SMYD2-mediated EML4-ALK methylation on the signaling pathway and growth in non-small-cell lung cancer cells. Cancer Sci. 2017;108:1203-1209 pubmed publisher
  9. Muscarella L, Rossi G, Trombetta D, la Torre A, Di Candia L, Mengoli M, et al. A malignant inflammatory myofibroblastic tumor of the hypopharynx harboring the 3a/b variants of the EML4-ALK fusion gene. Oncol Lett. 2017;13:593-598 pubmed publisher
  10. Lee J, Li C, Huang H, Zhu M, Mariño Enríquez A, Lee C, et al. ALK oncoproteins in atypical inflammatory myofibroblastic tumours: novel RRBP1-ALK fusions in epithelioid inflammatory myofibroblastic sarcoma. J Pathol. 2017;241:316-323 pubmed publisher
  11. von Laffert M, Hänel M, Dietel M, Anagnostopoulos I, Johrens K. Increase of T and B cells and altered BACH2 expression patterns in bone marrow trephines of imatinib-treated patients with chronic myelogenous leukaemia. Oncol Lett. 2016;12:2421-2428 pubmed
  12. Morena D, Maestro N, Bersani F, Forni P, Lingua M, Foglizzo V, et al. Hepatocyte Growth Factor-mediated satellite cells niche perturbation promotes development of distinct sarcoma subtypes. elife. 2016;5: pubmed publisher
  13. Lakschevitz F, Hassanpour S, Rubin A, Fine N, Sun C, Glogauer M. Identification of neutrophil surface marker changes in health and inflammation using high-throughput screening flow cytometry. Exp Cell Res. 2016;342:200-9 pubmed publisher
  14. Ardini E, Menichincheri M, Banfi P, Bosotti R, De Ponti C, Pulci R, et al. Entrectinib, a Pan-TRK, ROS1, and ALK Inhibitor with Activity in Multiple Molecularly Defined Cancer Indications. Mol Cancer Ther. 2016;15:628-39 pubmed publisher
  15. Ceccon M, Merlo M, Mologni L, Poggio T, Varesio L, Menotti M, et al. Excess of NPM-ALK oncogenic signaling promotes cellular apoptosis and drug dependency. Oncogene. 2016;35:3854-3865 pubmed publisher
  16. Debruyne D, Bhatnagar N, Sharma B, Luther W, Moore N, Cheung N, et al. ALK inhibitor resistance in ALK(F1174L)-driven neuroblastoma is associated with AXL activation and induction of EMT. Oncogene. 2016;35:3681-91 pubmed publisher
  17. Davare M, Vellore N, Wagner J, Eide C, Goodman J, Drilon A, et al. Structural insight into selectivity and resistance profiles of ROS1 tyrosine kinase inhibitors. Proc Natl Acad Sci U S A. 2015;112:E5381-90 pubmed publisher
  18. He D, Chen H, Muramatsu H, Lasek A. Ethanol activates midkine and anaplastic lymphoma kinase signaling in neuroblastoma cells and in the brain. J Neurochem. 2015;135:508-21 pubmed publisher
  19. Vishwamitra D, Curry C, Alkan S, Song Y, Gallick G, Kaseb A, et al. The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK⁺ T-cell lymphoma. Mol Cancer. 2015;14:53 pubmed publisher
  20. Chen Q, Gu Y, Liu B. Clinicopathological characteristics of kidney mucinous tubular and spindle cell carcinoma. Int J Clin Exp Pathol. 2015;8:1007-12 pubmed
  21. Papadakis A, Sun C, Knijnenburg T, Xue Y, Grernrum W, Hölzel M, et al. SMARCE1 suppresses EGFR expression and controls responses to MET and ALK inhibitors in lung cancer. Cell Res. 2015;25:445-58 pubmed publisher
  22. Onaindia A, Montes Moreno S, Rodriguez Pinilla S, Batlle A, Gonzalez de Villambrosia S, Rodriguez A, et al. Primary cutaneous anaplastic large cell lymphomas with 6p25.3 rearrangement exhibit particular histological features. Histopathology. 2015;66:846-55 pubmed publisher
  23. George S, Vishwamitra D, Manshouri R, Shi P, Amin H. The ALK inhibitor ASP3026 eradicates NPM-ALK? T-cell anaplastic large-cell lymphoma in vitro and in a systemic xenograft lymphoma model. Oncotarget. 2014;5:5750-63 pubmed
  24. Yang Y, Li Y, Liu Y, Yang M, Liu K. CD30+ extranodal natural killer/T-cell lymphoma mimicking phlegmonous myositis: A case report. Oncol Lett. 2014;7:1419-1421 pubmed
  25. Suzuki O, Abe M. Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: regulatory roles of cell surface glycans. Int J Oncol. 2014;44:1433-42 pubmed publisher
  26. Kishida S, Mu P, Miyakawa S, Fujiwara M, Abe T, Sakamoto K, et al. Midkine promotes neuroblastoma through Notch2 signaling. Cancer Res. 2013;73:1318-27 pubmed publisher
  27. Cheng M, Quail M, Gingrich D, Ott G, Lu L, Wan W, et al. CEP-28122, a highly potent and selective orally active inhibitor of anaplastic lymphoma kinase with antitumor activity in experimental models of human cancers. Mol Cancer Ther. 2012;11:670-9 pubmed publisher
  28. Petrilli G, Lorenzi L, Paracchini R, Ubiali A, Schumacher R, Cabassa P, et al. Epstein-Barr virus-associated adrenal smooth muscle tumors and disseminated diffuse large B-cell lymphoma in a child with common variable immunodeficiency: a case report and review of the literature. Int J Surg Pathol. 2014;22:712-21 pubmed publisher
  29. Piva R, Chiarle R, Manazza A, Taulli R, Simmons W, Ambrogio C, et al. Ablation of oncogenic ALK is a viable therapeutic approach for anaplastic large-cell lymphomas. Blood. 2006;107:689-97 pubmed