This is a Validated Antibody Database (VAD) review about human AIF, based on 37 published articles (read how Labome selects the articles), using AIF antibody in all methods. It is aimed to help Labome visitors find the most suited AIF antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
AIF synonym: AIF; AUNX1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8; SEMDHL

Santa Cruz Biotechnology
mouse monoclonal (E-11)
  • western blot; human; loading ...; fig 1c
Santa Cruz Biotechnology AIF antibody (Santa Cruz, sc-390619) was used in western blot on human samples (fig 1c). J Cell Biol (2022) ncbi
mouse monoclonal (E-1)
  • immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 4, 5, 7
  • western blot; human; 1:250; loading ...; fig 2
Santa Cruz Biotechnology AIF antibody (Santa Cruz, sc-13116) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 4, 5, 7) and in western blot on human samples at 1:250 (fig 2). Cancers (Basel) (2021) ncbi
mouse monoclonal (E-1)
  • western blot; human; 1:1000; loading ...; fig 4b
Santa Cruz Biotechnology AIF antibody (Santa, sc-13116) was used in western blot on human samples at 1:1000 (fig 4b). EMBO Mol Med (2019) ncbi
mouse monoclonal (E-1)
  • western blot; human; fig 1b
In order to show the role of Pex3 and Pex14 in generating human peroxisomes, Santa Cruz Biotechnology AIF antibody (Santa Cruz, sc-13116) was used in western blot on human samples (fig 1b). Nature (2017) ncbi
mouse monoclonal (E-1)
  • western blot; human; 1:700; loading ...; fig 4a
Santa Cruz Biotechnology AIF antibody (Santa Cruz, sc-13116) was used in western blot on human samples at 1:700 (fig 4a). Oncotarget (2016) ncbi
mouse monoclonal (E-1)
  • western blot; human; fig 2
In order to analyze control of AIF-dependent growth support in pancreatic cancer cells by basal metabolic state, Santa Cruz Biotechnology AIF antibody (Santa Cruz Biotechnology, sc-13116) was used in western blot on human samples (fig 2). BMC Cancer (2016) ncbi
mouse monoclonal (E-1)
  • western blot; human; fig s8
In order to study the support of redox homeostasis during anchorage-independent growth by reductive carboxylation, Santa Cruz Biotechnology AIF antibody (Santa Cruz, sc-13116) was used in western blot on human samples (fig s8). Nature (2016) ncbi
mouse monoclonal (E-1)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Santa Cruz Biotechnology AIF antibody (SCBT, E-1) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (E-1)
  • western blot; rat; fig 2
  • western blot; mouse; fig 6
Santa Cruz Biotechnology AIF antibody (Santa Cruz, sc-13116) was used in western blot on rat samples (fig 2) and in western blot on mouse samples (fig 6). Cell Death Differ (2016) ncbi
mouse monoclonal (E-1)
  • western blot; human; 1:1000; fig 5
Santa Cruz Biotechnology AIF antibody (Santa Cruz Biotechnology, sc-13116) was used in western blot on human samples at 1:1000 (fig 5). Oncotarget (2015) ncbi
mouse monoclonal (E-1)
  • immunocytochemistry; human; tbl 1
  • western blot; human; 1:1000; tbl 1
Santa Cruz Biotechnology AIF antibody (Santa Cruz, sc-13116) was used in immunocytochemistry on human samples (tbl 1) and in western blot on human samples at 1:1000 (tbl 1). Methods Mol Biol (2015) ncbi
mouse monoclonal (E-1)
  • western blot; human; fig 4
Santa Cruz Biotechnology AIF antibody (Santa Cruz, Sc-13116) was used in western blot on human samples (fig 4). Hum Exp Toxicol (2015) ncbi
mouse monoclonal (E-1)
  • western blot; human
  • western blot; mouse
  • western blot; rat
Santa Cruz Biotechnology AIF antibody (Santa Cruz Biotechnology, sc-13116) was used in western blot on human samples , in western blot on mouse samples and in western blot on rat samples . J Neurosci (2014) ncbi
mouse monoclonal (B-9)
  • immunohistochemistry - paraffin section; human; 1:200
  • western blot; human
Santa Cruz Biotechnology AIF antibody (Santa Cruz Biotech, sc-55519) was used in immunohistochemistry - paraffin section on human samples at 1:200 and in western blot on human samples . PLoS ONE (2014) ncbi
Invitrogen
mouse monoclonal (4E7E11)
  • proximity ligation assay; mouse; fig 3
  • immunoprecipitation; mouse; fig 1a
  • immunocytochemistry; mouse; fig 2a
  • western blot; mouse; fig 1c
In order to discover mitochondrial connexin 43 protein-protein interactions in mouse heart, Invitrogen AIF antibody (Pierce-Thermo Scientific, MA5-15880) was used in proximity ligation assay on mouse samples (fig 3), in immunoprecipitation on mouse samples (fig 1a), in immunocytochemistry on mouse samples (fig 2a) and in western blot on mouse samples (fig 1c). J Cell Mol Med (2016) ncbi
mouse monoclonal (4E7E11)
  • western blot; human; 1:4000
In order to discuss the known phenotypes related to AIFM1 mutations and further study a single patient, Invitrogen AIF antibody (Pierce, MA5-15880) was used in western blot on human samples at 1:4000. Mitochondrion (2015) ncbi
Abcam
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:350; loading ...; fig 7a
Abcam AIF antibody (Abcam, ab1998) was used in immunohistochemistry - frozen section on mouse samples at 1:350 (fig 7a). J Comp Neurol (2019) ncbi
R&D Systems
domestic sheep polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:300; loading ...; fig 5c
R&D Systems AIF antibody (R&D, AF5824) was used in immunohistochemistry - paraffin section on mouse samples at 1:300 (fig 5c). Int J Mol Sci (2021) ncbi
Rockland Immunochemicals
domestic rabbit polyclonal
Rockland Immunochemicals AIF antibody (Rockland Immunochemicals, 200-401-985) was used . Oncol Rep (2015) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D39D2)
  • immunocytochemistry; rat; 1:200; loading ...; fig 2k
Cell Signaling Technology AIF antibody (CST, 5318s) was used in immunocytochemistry on rat samples at 1:200 (fig 2k). Front Pharmacol (2022) ncbi
domestic rabbit monoclonal (D39D2)
  • western blot; human; 1:1000; loading ...; fig 3f
Cell Signaling Technology AIF antibody (Cell Signaling, 5318) was used in western blot on human samples at 1:1000 (fig 3f). Nat Commun (2022) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 4
Cell Signaling Technology AIF antibody (Cell Signaling Technology, 4642) was used in immunohistochemistry on mouse samples (fig 4). Int J Mol Sci (2021) ncbi
domestic rabbit monoclonal (D39D2)
  • immunocytochemistry; mouse; loading ...; fig s5b
Cell Signaling Technology AIF antibody (Cell Signaling, 5318) was used in immunocytochemistry on mouse samples (fig s5b). Nat Commun (2021) ncbi
domestic rabbit monoclonal (D39D2)
  • western blot; human; 1:1000; loading ...; fig s3m
Cell Signaling Technology AIF antibody (Cell Signaling, 5318) was used in western blot on human samples at 1:1000 (fig s3m). Adv Sci (Weinh) (2021) ncbi
domestic rabbit monoclonal (D39D2)
  • immunocytochemistry; human; loading ...; fig s1
Cell Signaling Technology AIF antibody (Cell Signaling Technology, 5318) was used in immunocytochemistry on human samples (fig s1). Autophagy (2019) ncbi
domestic rabbit monoclonal (D39D2)
  • western blot; West Nile virus; fig 6e
Cell Signaling Technology AIF antibody (Cell Signaling, 5318S) was used in western blot on West Nile virus samples (fig 6e). Nat Microbiol (2019) ncbi
domestic rabbit monoclonal (D39D2)
  • western blot; human; loading ...; fig 5l
Cell Signaling Technology AIF antibody (Cell Signaling, 5318) was used in western blot on human samples (fig 5l). Nat Cell Biol (2019) ncbi
domestic rabbit monoclonal (D39D2)
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 4b
Cell Signaling Technology AIF antibody (Cell Signaling, 5318) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 4b). Nucleic Acids Res (2019) ncbi
domestic rabbit monoclonal (D39D2)
  • western blot; mouse; 1:1000; loading ...; fig 7e
Cell Signaling Technology AIF antibody (Cell Signaling, 5318) was used in western blot on mouse samples at 1:1000 (fig 7e). Diabetologia (2019) ncbi
domestic rabbit monoclonal (D39D2)
  • western blot; human; loading ...; fig 1d
Cell Signaling Technology AIF antibody (Cell Signaling, 5318) was used in western blot on human samples (fig 1d). Mol Cell Biochem (2019) ncbi
domestic rabbit monoclonal (D39D2)
  • immunohistochemistry; human; 1:1500; loading ...; fig s2b
Cell Signaling Technology AIF antibody (CST, 5318S) was used in immunohistochemistry on human samples at 1:1500 (fig s2b). Sci Adv (2018) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:500; fig 5
Cell Signaling Technology AIF antibody (Cell Signaling, 4642) was used in western blot on rat samples at 1:500 (fig 5). Exp Ther Med (2016) ncbi
domestic rabbit monoclonal (D39D2)
  • western blot; mouse; 1:1000; fig 5
In order to learn about protection against acetaminophen hepatotoxicity by mitochondria-targeted antioxidant Mito-Tempo, Cell Signaling Technology AIF antibody (Cell Signaling Technology, 5318) was used in western blot on mouse samples at 1:1000 (fig 5). Arch Toxicol (2017) ncbi
domestic rabbit monoclonal (D39D2)
  • western blot; mouse; 1:1000; fig 3
Cell Signaling Technology AIF antibody (Cell Signaling Technology, 5318) was used in western blot on mouse samples at 1:1000 (fig 3). PLoS ONE (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; fig 2e
Cell Signaling Technology AIF antibody (Cell Signaling, 4642) was used in immunohistochemistry on mouse samples at 1:100 (fig 2e). Science (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig 3
In order to study increased dissociation of hexokinase II from mitochondrial outer membrane by overexpression or ErbB2 rendering breast cancer cells susceptible to 3-BrPA, Cell Signaling Technology AIF antibody (Cell signaling, 4642) was used in western blot on human samples at 1:1000 (fig 3). Oncol Lett (2016) ncbi
domestic rabbit monoclonal (D39D2)
  • immunocytochemistry; human; 1:400
  • western blot; human; 1:1000
Cell Signaling Technology AIF antibody (Cell Signaling, D39D2) was used in immunocytochemistry on human samples at 1:400 and in western blot on human samples at 1:1000. Mol Oncol (2015) ncbi
Articles Reviewed
  1. Zheng C, Xuan W, Chen Z, Zhang R, Huang X, Zhu Y, et al. CX3CL1 Worsens Cardiorenal Dysfunction and Serves as a Therapeutic Target of Canagliflozin for Cardiorenal Syndrome. Front Pharmacol. 2022;13:848310 pubmed publisher
  2. Jiang Q, Zhang X, Dai X, Han S, Wu X, Wang L, et al. S6K1-mediated phosphorylation of PDK1 impairs AKT kinase activity and oncogenic functions. Nat Commun. 2022;13:1548 pubmed publisher
  3. Cardamone M, Gao Y, Kwan J, Hayashi V, Sheeran M, Xu J, et al. Neuralized-like protein 4 (NEURL4) mediates ADP-ribosylation of mitochondrial proteins. J Cell Biol. 2022;221: pubmed publisher
  4. Paštar V, Lozić M, Kelam N, Filipović N, Bernard B, Katsuyama Y, et al. Connexin Expression Is Altered in Liver Development of Yotari (dab1 -/-) Mice. Int J Mol Sci. 2021;22: pubmed publisher
  5. Zhao H, Tang J, Chen H, Gu W, Geng H, Wang L, et al. 14,15-EET Reduced Brain Injury from Cerebral Ischemia and Reperfusion via Suppressing Neuronal Parthanatos. Int J Mol Sci. 2021;22: pubmed publisher
  6. Scagliola A, Miluzio A, Ventura G, Oliveto S, Cordiglieri C, Manfrini N, et al. Targeting of eIF6-driven translation induces a metabolic rewiring that reduces NAFLD and the consequent evolution to hepatocellular carcinoma. Nat Commun. 2021;12:4878 pubmed publisher
  7. Guo J, Cheng J, Zheng N, Zhang X, Dai X, Zhang L, et al. Copper Promotes Tumorigenesis by Activating the PDK1-AKT Oncogenic Pathway in a Copper Transporter 1 Dependent Manner. Adv Sci (Weinh). 2021;8:e2004303 pubmed publisher
  8. Letkovska K, Babal P, Cierna Z, Schmidtova S, Lišková V, Kalavska K, et al. Prognostic Value of Apoptosis-Inducing Factor (AIF) in Germ Cell Tumors. Cancers (Basel). 2021;13: pubmed publisher
  9. Power M, Rogerson L, Schubert T, Berens P, Euler T, Paquet Durand F. Systematic spatiotemporal mapping reveals divergent cell death pathways in three mouse models of hereditary retinal degeneration. J Comp Neurol. 2019;: pubmed publisher
  10. Ding X, Jiang X, Tian R, Zhao P, Li L, Wang X, et al. RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells. Autophagy. 2019;:1-13 pubmed publisher
  11. Li M, Johnson J, Truong B, Kim G, Weinbren N, Dittmar M, et al. Identification of antiviral roles for the exon-junction complex and nonsense-mediated decay in flaviviral infection. Nat Microbiol. 2019;4:985-995 pubmed publisher
  12. Guo J, Dai X, Laurent B, Zheng N, Gan W, Zhang J, et al. AKT methylation by SETDB1 promotes AKT kinase activity and oncogenic functions. Nat Cell Biol. 2019;21:226-237 pubmed publisher
  13. Ding D, Liu J, Dong K, Melnick A, Latham K, Chen C. Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. Nucleic Acids Res. 2019;47:2594-2608 pubmed publisher
  14. Signes A, Cerutti R, Dickson A, Benincá C, Hinchy E, Ghezzi D, et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med. 2019;11: pubmed publisher
  15. Yoshitake S, Murakami T, Suzuma K, Yoshitake T, Uji A, Morooka S, et al. Anti-fumarase antibody promotes the dropout of photoreceptor inner and outer segments in diabetic macular oedema. Diabetologia. 2019;62:504-516 pubmed publisher
  16. Killackey S, Rahman M, Soares F, Zhang A, Abdel Nour M, Philpott D, et al. The mitochondrial Nod-like receptor NLRX1 modifies apoptosis through SARM1. Mol Cell Biochem. 2019;453:187-196 pubmed publisher
  17. NGUYEN J, Ray C, Fox A, Mendonça D, Kim J, Krebsbach P. Mammalian EAK-7 activates alternative mTOR signaling to regulate cell proliferation and migration. Sci Adv. 2018;4:eaao5838 pubmed publisher
  18. Sugiura A, Mattie S, Prudent J, McBride H. Newly born peroxisomes are a hybrid of mitochondrial and ER-derived pre-peroxisomes. Nature. 2017;542:251-254 pubmed publisher
  19. Lu H, Yang X, Tian X, Tang S, Li L, Zhao S, et al. The in vitro and vivo anti-tumor effects and molecular mechanisms of suberoylanilide hydroxamic acid (SAHA) and MG132 on the aggressive phenotypes of gastric cancer cells. Oncotarget. 2016;7:56508-56525 pubmed publisher
  20. Scott A, Wilkinson A, Wilkinson J. Basal metabolic state governs AIF-dependent growth support in pancreatic cancer cells. BMC Cancer. 2016;16:286 pubmed publisher
  21. Jeong J, Noh M, Choi J, Lee H, Kim S. Neuroprotective and antioxidant activities of bamboo salt soy sauce against H2O2-induced oxidative stress in rat cortical neurons. Exp Ther Med. 2016;11:1201-1210 pubmed
  22. Jiang L, Shestov A, Swain P, Yang C, Parker S, Wang Q, et al. Reductive carboxylation supports redox homeostasis during anchorage-independent growth. Nature. 2016;532:255-8 pubmed publisher
  23. Du K, Farhood A, Jaeschke H. Mitochondria-targeted antioxidant Mito-Tempo protects against acetaminophen hepatotoxicity. Arch Toxicol. 2017;91:761-773 pubmed publisher
  24. Nagase M, Kurihara H, Aiba A, Young M, Sakai T. Deletion of Rac1GTPase in the Myeloid Lineage Protects against Inflammation-Mediated Kidney Injury in Mice. PLoS ONE. 2016;11:e0150886 pubmed publisher
  25. Lei L, Spradling A. Mouse oocytes differentiate through organelle enrichment from sister cyst germ cells. Science. 2016;352:95-9 pubmed publisher
  26. Denuc A, Núñez E, Calvo E, Loureiro M, Miro Casas E, Guarás A, et al. New protein-protein interactions of mitochondrial connexin 43 in mouse heart. J Cell Mol Med. 2016;20:794-803 pubmed publisher
  27. Gao S, Chen X, Jin H, Ren S, Liu Z, Fang X, et al. Overexpression of ErbB2 renders breast cancer cells susceptible to 3-BrPA through the increased dissociation of hexokinase II from mitochondrial outer membrane. Oncol Lett. 2016;11:1567-1573 pubmed
  28. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  29. Sabirzhanov B, Stoica B, Zhao Z, Loane D, Wu J, Dorsey S, et al. miR-711 upregulation induces neuronal cell death after traumatic brain injury. Cell Death Differ. 2016;23:654-68 pubmed publisher
  30. Koh D, Powell D, Blake S, Hoffman J, Hopkins M, Feng X. Enhanced cytotoxicity in triple-negative and estrogen receptor‑positive breast adenocarcinoma cells due to inhibition of the transient receptor potential melastatin-2 channel. Oncol Rep. 2015;34:1589-98 pubmed publisher
  31. Loureiro R, Magalhães Novais S, Mesquita K, Baldeiras I, Sousa I, Tavares L, et al. Melatonin antiproliferative effects require active mitochondrial function in embryonal carcinoma cells. Oncotarget. 2015;6:17081-96 pubmed
  32. Kettwig M, Schubach M, Zimmermann F, Klinge L, Mayr J, Biskup S, et al. From ventriculomegaly to severe muscular atrophy: expansion of the clinical spectrum related to mutations in AIFM1. Mitochondrion. 2015;21:12-8 pubmed publisher
  33. Vega Naredo I, Cunha Oliveira T, Serafim T, Sardao V, Oliveira P. Analysis of pro-apoptotic protein trafficking to and from mitochondria. Methods Mol Biol. 2015;1241:163-80 pubmed publisher
  34. Iwaniuk A, Jabłońska E, Jabłoński J, Ratajczak Wrona W, Garley M. Expression of selected proteins of the extrinsic and intrinsic pathways of apoptosis in human leukocytes exposed to N-nitrosodimethylamine. Hum Exp Toxicol. 2015;34:591-600 pubmed publisher
  35. Passaro C, Volpe M, Botta G, Scamardella E, Perruolo G, Gillespie D, et al. PARP inhibitor olaparib increases the oncolytic activity of dl922-947 in in vitro and in vivo model of anaplastic thyroid carcinoma. Mol Oncol. 2015;9:78-92 pubmed publisher
  36. Sabirzhanov B, Zhao Z, Stoica B, Loane D, Wu J, Borroto C, et al. Downregulation of miR-23a and miR-27a following experimental traumatic brain injury induces neuronal cell death through activation of proapoptotic Bcl-2 proteins. J Neurosci. 2014;34:10055-71 pubmed publisher
  37. Xu S, Wu H, Nie H, Yue L, Jiang H, Xiao S, et al. AIF downregulation and its interaction with STK3 in renal cell carcinoma. PLoS ONE. 2014;9:e100824 pubmed publisher