This is a Validated Antibody Database (VAD) review about human AGO2, based on 58 published articles (read how Labome selects the articles), using AGO2 antibody in all methods. It is aimed to help Labome visitors find the most suited AGO2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
AGO2 synonym: CASC7; EIF2C2; LINC00980; PPD; Q10

Knockout validation
MilliporeSigma
rat monoclonal (11A9)
  • western blot knockout validation; human; loading ...; fig 4c
  • chromatin immunoprecipitation; human; loading ...; fig 3a
MilliporeSigma AGO2 antibody (Sigma-Aldrich, SAB4200085) was used in western blot knockout validation on human samples (fig 4c) and in chromatin immunoprecipitation on human samples (fig 3a). Sci Rep (2017) ncbi
MilliporeSigma
rat monoclonal (11A9)
  • western blot knockout validation; human; loading ...; fig s3a
MilliporeSigma AGO2 antibody (Sigma, SAB4200085) was used in western blot knockout validation on human samples (fig s3a). Nature (2017) ncbi
EMD Millipore
rat monoclonal (11A9)
  • western blot knockout validation; human; loading ...; fig 3b
  • immunoprecipitation; human
EMD Millipore AGO2 antibody (Millipore, 11A9) was used in western blot knockout validation on human samples (fig 3b) and in immunoprecipitation on human samples . Nucleic Acids Res (2017) ncbi
Abcam
domestic rabbit monoclonal (EPR10411)
  • western blot; human; loading ...; fig s3c, 3b, 3c
Abcam AGO2 antibody (Abcam, ab186733) was used in western blot on human samples (fig s3c, 3b, 3c). Cell (2019) ncbi
mouse monoclonal (2E12-1C9)
  • RNA immunoprecipitation; human; loading ...; fig 6a
Abcam AGO2 antibody (Abcam, ab57113) was used in RNA immunoprecipitation on human samples (fig 6a). Nucleic Acids Res (2018) ncbi
domestic rabbit monoclonal (EPR10411)
  • RNA immunoprecipitation; human; loading ...; fig 2b
Abcam AGO2 antibody (Abcam, ab186733) was used in RNA immunoprecipitation on human samples (fig 2b). Cell Physiol Biochem (2018) ncbi
mouse monoclonal (2E12-1C9)
Abcam AGO2 antibody (Abcam, ab57113) was used . Proc Natl Acad Sci U S A (2018) ncbi
domestic rabbit polyclonal
  • immunoprecipitation; mouse; 1:1000; loading ...; fig 2b
Abcam AGO2 antibody (Abcam, ab32381) was used in immunoprecipitation on mouse samples at 1:1000 (fig 2b). Mol Ther Nucleic Acids (2018) ncbi
mouse monoclonal (2E12-1C9)
  • immunoprecipitation; human; fig 4a
  • western blot; human; 1:1000; fig 4a
Abcam AGO2 antibody (Abcam, ab57113) was used in immunoprecipitation on human samples (fig 4a) and in western blot on human samples at 1:1000 (fig 4a). Mol Ther (2017) ncbi
Abnova
mouse monoclonal (2E12-1C9)
  • western blot; human; loading ...; fig s3a
Abnova AGO2 antibody (Abnova, H00027161-M01) was used in western blot on human samples (fig s3a). Cell (2019) ncbi
mouse polyclonal
  • western blot; human; loading ...; fig s6g
Abnova AGO2 antibody (Abnova, H00027161-A01) was used in western blot on human samples (fig s6g). Cell (2019) ncbi
mouse monoclonal (2E12-1C9)
  • immunocytochemistry; human; loading ...; fig 1c
  • western blot; human; loading ...; fig s12b
In order to analyze if the conserved DEAD-box protein DDX6 is commonly present in the nuclei of human-derived cells, Abnova AGO2 antibody (Abnova, H00027161-M01) was used in immunocytochemistry on human samples (fig 1c) and in western blot on human samples (fig s12b). Sci Rep (2017) ncbi
mouse monoclonal (2E12-1C9)
  • chromatin immunoprecipitation; human; fig s1
Abnova AGO2 antibody (Abnova, 2E12-1C9) was used in chromatin immunoprecipitation on human samples (fig s1). Oncotarget (2016) ncbi
mouse monoclonal (2E12-1C9)
  • immunoprecipitation; mouse
Abnova AGO2 antibody (Abnova, H00027161-M01) was used in immunoprecipitation on mouse samples . PLoS ONE (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (4F9)
  • chromatin immunoprecipitation; human; loading ...; fig 3b
Santa Cruz Biotechnology AGO2 antibody (SantaCruz, sc-53521) was used in chromatin immunoprecipitation on human samples (fig 3b). Sci Rep (2017) ncbi
mouse monoclonal (4F9)
  • western blot; mouse; fig 7
Santa Cruz Biotechnology AGO2 antibody (Santa Cruz, sc-53521) was used in western blot on mouse samples (fig 7). Autophagy (2015) ncbi
Invitrogen
domestic rabbit monoclonal (R.386.2)
  • RNA immunoprecipitation; human; fig s11
In order to identify an atheroprotective role for circular RNAs, Invitrogen AGO2 antibody (Thermo Scientific, MA5-14861) was used in RNA immunoprecipitation on human samples (fig s11). Nat Commun (2016) ncbi
domestic rabbit monoclonal (R.386.2)
  • immunoprecipitation; mouse; fig s2
In order to elucidate miR-21 that induces fibrosis in an acute cardiac allograft transplantation model, Invitrogen AGO2 antibody (Thermo Pierce, MA5-14861) was used in immunoprecipitation on mouse samples (fig s2). Cardiovasc Res (2016) ncbi
MilliporeSigma
rat monoclonal (11A9)
  • western blot; human; 1:1000; loading ...; fig 2d
MilliporeSigma AGO2 antibody (Sigma, 11A9) was used in western blot on human samples at 1:1000 (fig 2d). Nucleic Acids Res (2019) ncbi
rat monoclonal (11A9)
  • western blot; human; loading ...; fig s3g, 3b, 3c, s3a
MilliporeSigma AGO2 antibody (Sigma, SAB4200085) was used in western blot on human samples (fig s3g, 3b, 3c, s3a). Cell (2019) ncbi
mouse monoclonal (2E12-1C9)
  • immunoprecipitation; mouse; loading ...; fig 2a
MilliporeSigma AGO2 antibody (Sigma-Aldrich, WH0027161M1) was used in immunoprecipitation on mouse samples (fig 2a). Nat Commun (2017) ncbi
rat monoclonal (11A9)
  • western blot knockout validation; human; loading ...; fig 4c
  • chromatin immunoprecipitation; human; loading ...; fig 3a
MilliporeSigma AGO2 antibody (Sigma-Aldrich, SAB4200085) was used in western blot knockout validation on human samples (fig 4c) and in chromatin immunoprecipitation on human samples (fig 3a). Sci Rep (2017) ncbi
rat monoclonal (11A9)
  • immunoprecipitation; rat; fig 4h
MilliporeSigma AGO2 antibody (Sigma-Aldrich, SAB4200085)) was used in immunoprecipitation on rat samples (fig 4h). Mol Neurobiol (2018) ncbi
rat monoclonal (11A9)
  • western blot knockout validation; human; loading ...; fig s3a
MilliporeSigma AGO2 antibody (Sigma, SAB4200085) was used in western blot knockout validation on human samples (fig s3a). Nature (2017) ncbi
rat monoclonal (11A9)
  • immunoprecipitation; human; fig 4
MilliporeSigma AGO2 antibody (Sigma, SAB4200085) was used in immunoprecipitation on human samples (fig 4). Nat Commun (2016) ncbi
rat monoclonal (11A9)
  • western blot; human; 1:2000; loading ...; fig 4b
MilliporeSigma AGO2 antibody (Sigma-Aldrich, SAB4200085) was used in western blot on human samples at 1:2000 (fig 4b). Sci Transl Med (2016) ncbi
rat monoclonal (11A9)
  • western blot; human; 1:1000; fig s8
MilliporeSigma AGO2 antibody (Sigma, SAB4200085) was used in western blot on human samples at 1:1000 (fig s8). Nat Commun (2016) ncbi
rat monoclonal (11A9)
  • RNA immunoprecipitation; human
In order to develop reagents to study RNA binding proteins, MilliporeSigma AGO2 antibody (Sigma, SAB4200085) was used in RNA immunoprecipitation on human samples . Mol Cell (2016) ncbi
rat monoclonal (11A9)
  • western blot; human; fig 2c
MilliporeSigma AGO2 antibody (Sigma, SAB4200085) was used in western blot on human samples (fig 2c). Sci Rep (2016) ncbi
rat monoclonal (11A9)
  • immunocytochemistry; mouse
  • western blot; mouse
MilliporeSigma AGO2 antibody (Sigma, 11A9) was used in immunocytochemistry on mouse samples and in western blot on mouse samples . FEBS Lett (2014) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (C34C6)
  • immunoprecipitation; mouse; 1:1000; loading ...; fig 2b
  • western blot; mouse; 1:1000; loading ...; fig 2a
Cell Signaling Technology AGO2 antibody (CST, 2897) was used in immunoprecipitation on mouse samples at 1:1000 (fig 2b) and in western blot on mouse samples at 1:1000 (fig 2a). Mol Ther Nucleic Acids (2018) ncbi
domestic rabbit monoclonal (C34C6)
  • western blot; rat; 1:1000; loading ...; fig 4b
Cell Signaling Technology AGO2 antibody (Cell Signaling, C34C6) was used in western blot on rat samples at 1:1000 (fig 4b). EMBO J (2018) ncbi
domestic rabbit monoclonal (C34C6)
Cell Signaling Technology AGO2 antibody (Cell Signaling Technology, C34C6) was used . Nucleic Acids Res (2018) ncbi
domestic rabbit monoclonal (C34C6)
  • western blot; mouse; loading ...; fig 1e
Cell Signaling Technology AGO2 antibody (Cell Signaling, C34C6) was used in western blot on mouse samples (fig 1e). Nat Commun (2017) ncbi
domestic rabbit monoclonal (C34C6)
  • RNA immunoprecipitation; human; loading ...; fig 4c
Cell Signaling Technology AGO2 antibody (Cell Signaling, 2897S) was used in RNA immunoprecipitation on human samples (fig 4c). Sci Transl Med (2016) ncbi
domestic rabbit monoclonal (C34C6)
  • western blot; human; fig s3b
Cell Signaling Technology AGO2 antibody (Cell Signaling, 2897) was used in western blot on human samples (fig s3b). Nucleic Acids Res (2016) ncbi
domestic rabbit monoclonal (C34C6)
  • western blot; rat; 1:500; loading ...; fig 5
Cell Signaling Technology AGO2 antibody (cell signalling, C34C6) was used in western blot on rat samples at 1:500 (fig 5). Mol Neurobiol (2017) ncbi
domestic rabbit monoclonal (C34C6)
  • western blot; human; loading ...; fig 7c
Cell Signaling Technology AGO2 antibody (Cell Signaling, 2897) was used in western blot on human samples (fig 7c). Nat Commun (2015) ncbi
domestic rabbit monoclonal (C34C6)
  • western blot; mouse; fig 3a
Cell Signaling Technology AGO2 antibody (Cell Signaling, C34C6) was used in western blot on mouse samples (fig 3a). RNA (2015) ncbi
domestic rabbit monoclonal (C34C6)
  • western blot; mouse; fig 6a
Cell Signaling Technology AGO2 antibody (Cell Signaling, C34C6) was used in western blot on mouse samples (fig 6a). J Biol Chem (2015) ncbi
domestic rabbit monoclonal (C34C6)
  • western blot; human; 1:1000; fig 3
In order to elucidate how post-translational modification of ATAXIN1 levels contributes to spinocerebellar ataxia type 1, Cell Signaling Technology AGO2 antibody (Cell Signaling Technology, 2897S) was used in western blot on human samples at 1:1000 (fig 3). Cell (2015) ncbi
domestic rabbit monoclonal (C34C6)
  • western blot; human
Cell Signaling Technology AGO2 antibody (Cell Signaling Technology, C34C6) was used in western blot on human samples . J Biol Chem (2015) ncbi
domestic rabbit monoclonal (C34C6)
  • western blot; human
Cell Signaling Technology AGO2 antibody (Cell signaling, 2897) was used in western blot on human samples . Nucleic Acids Res (2014) ncbi
domestic rabbit monoclonal (C34C6)
  • western blot; human
Cell Signaling Technology AGO2 antibody (Cell Signaling, 2897) was used in western blot on human samples . Cell Death Dis (2013) ncbi
domestic rabbit monoclonal (C34C6)
  • western blot; rat; 1:1000
Cell Signaling Technology AGO2 antibody (Cell Signalling Technology, 2897) was used in western blot on rat samples at 1:1000. Addict Biol (2015) ncbi
Wako Chemicals USA
monoclonal
  • immunoprecipitation; mouse; fig 3
Wako Chemicals USA AGO2 antibody (Wako, 018-22021) was used in immunoprecipitation on mouse samples (fig 3). Sci Rep (2016) ncbi
mouse monoclonal (4G8)
  • immunocytochemistry; human; fig s4
  • western blot; human; fig 3
Wako Chemicals USA AGO2 antibody (Wako, 4G8) was used in immunocytochemistry on human samples (fig s4) and in western blot on human samples (fig 3). Nucleic Acids Res (2015) ncbi
mouse monoclonal (4G8)
  • immunoprecipitation; human; fig 4
Wako Chemicals USA AGO2 antibody (Wako Chemicals, 015-22031) was used in immunoprecipitation on human samples (fig 4). Nucleic Acids Res (2015) ncbi
mouse monoclonal (4G8)
  • immunoprecipitation; human
  • immunocytochemistry; human
  • western blot; human
Wako Chemicals USA AGO2 antibody (Wako, 015-22031) was used in immunoprecipitation on human samples , in immunocytochemistry on human samples and in western blot on human samples . Mol Cell Biol (2014) ncbi
monoclonal
  • immunoprecipitation; human; fig 2b
  • immunoprecipitation; mouse; fig 2b
  • western blot; mouse; fig 2c
Wako Chemicals USA AGO2 antibody (Wako, 01822021) was used in immunoprecipitation on human samples (fig 2b), in immunoprecipitation on mouse samples (fig 2b) and in western blot on mouse samples (fig 2c). Mol Cell (2014) ncbi
mouse monoclonal (4G8)
  • immunoprecipitation; human
Wako Chemicals USA AGO2 antibody (Wako, 011-22033) was used in immunoprecipitation on human samples . Biochemistry (2013) ncbi
mouse monoclonal (4G8)
  • immunoprecipitation; human
Wako Chemicals USA AGO2 antibody (Wako, 011-22033) was used in immunoprecipitation on human samples . Nucleic Acids Res (2013) ncbi
mouse monoclonal (4G8)
  • immunoprecipitation; human
Wako Chemicals USA AGO2 antibody (Wako, 015-22031) was used in immunoprecipitation on human samples . Nucleic Acids Res (2013) ncbi
mouse monoclonal (4G8)
  • immunoprecipitation; human
Wako Chemicals USA AGO2 antibody (Wako Chemicals, 4G8) was used in immunoprecipitation on human samples . EMBO J (2013) ncbi
mouse monoclonal (4G8)
  • immunoprecipitation; human; 5 ul
Wako Chemicals USA AGO2 antibody (Wako, 015-22031) was used in immunoprecipitation on human samples at 5 ul. Nucleic Acids Res (2012) ncbi
mouse monoclonal (4G8)
  • immunocytochemistry; human; fig 2
Wako Chemicals USA AGO2 antibody (Wako, 011-22033) was used in immunocytochemistry on human samples (fig 2). J Virol (2011) ncbi
EMD Millipore
rat monoclonal (11A9)
  • western blot knockout validation; human; loading ...; fig 3b
  • immunoprecipitation; human
EMD Millipore AGO2 antibody (Millipore, 11A9) was used in western blot knockout validation on human samples (fig 3b) and in immunoprecipitation on human samples . Nucleic Acids Res (2017) ncbi
mouse monoclonal (9E8.2)
  • western blot; mouse; 1:2000; fig 6
EMD Millipore AGO2 antibody (Millipore, 04-642) was used in western blot on mouse samples at 1:2000 (fig 6). Genome Biol (2016) ncbi
mouse monoclonal
  • RNA immunoprecipitation; human; fig 8
EMD Millipore AGO2 antibody (Millipore, 03-110) was used in RNA immunoprecipitation on human samples (fig 8). Nucleic Acids Res (2016) ncbi
mouse monoclonal (2A8)
  • RNA immunoprecipitation; mouse; fig s6
In order to assess the efficiency and potential safety of miRNA replacement therapy for Alzheimer's disease, EMD Millipore AGO2 antibody (Millipore, MABE56) was used in RNA immunoprecipitation on mouse samples (fig s6). Mol Ther Nucleic Acids (2015) ncbi
mouse monoclonal (2A8)
  • immunoprecipitation; mouse; fig 2
EMD Millipore AGO2 antibody (Millipore, 2A8) was used in immunoprecipitation on mouse samples (fig 2). Nat Commun (2015) ncbi
mouse monoclonal (2A8)
  • immunoprecipitation; human; fig 4
EMD Millipore AGO2 antibody (Millipore, MABE56) was used in immunoprecipitation on human samples (fig 4). Nucleic Acids Res (2015) ncbi
mouse monoclonal (2A8)
  • chromatin immunoprecipitation; human
EMD Millipore AGO2 antibody (Millipore, MABE56) was used in chromatin immunoprecipitation on human samples . G3 (Bethesda) (2014) ncbi
mouse monoclonal
  • chromatin immunoprecipitation; human
EMD Millipore AGO2 antibody (Merk Millipore, 03-110) was used in chromatin immunoprecipitation on human samples . Cell Death Dis (2013) ncbi
mouse monoclonal (2A8)
  • immunoprecipitation; human
EMD Millipore AGO2 antibody (Millipore, MABE56) was used in immunoprecipitation on human samples . Proc Natl Acad Sci U S A (2013) ncbi
mouse monoclonal (2A8)
  • immunoprecipitation; mouse
EMD Millipore AGO2 antibody (Millipore, MABE56) was used in immunoprecipitation on mouse samples . J Biol Chem (2012) ncbi
mouse monoclonal
  • western blot; mouse
EMD Millipore AGO2 antibody (Upstate, 04-085) was used in western blot on mouse samples . Nature (2009) ncbi
MBL International
In order to develop reagents to study RNA binding proteins, MBL International AGO2 antibody (MBLI, RN003M) was used . Mol Cell (2016) ncbi
  • western blot; human; fig 1
MBL International AGO2 antibody (MBL, RN005M) was used in western blot on human samples (fig 1). Sci Rep (2015) ncbi
Articles Reviewed
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  2. Li Y, Wang L, Rivera Serrano E, Chen X, Lemon S. TNRC6 proteins modulate hepatitis C virus replication by spatially regulating the binding of miR-122/Ago2 complexes to viral RNA. Nucleic Acids Res. 2019;47:6411-6424 pubmed publisher
  3. Jeppesen D, Fenix A, Franklin J, Higginbotham J, Zhang Q, Zimmerman L, et al. Reassessment of Exosome Composition. Cell. 2019;177:428-445.e18 pubmed publisher
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  39. Gennarino V, Singh R, White J, De Maio A, Han K, Kim J, et al. Pumilio1 haploinsufficiency leads to SCA1-like neurodegeneration by increasing wild-type Ataxin1 levels. Cell. 2015;160:1087-98 pubmed publisher
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  41. Koo C, Kobiyama K, Shen Y, LeBert N, Ahmad S, Khatoo M, et al. RNA polymerase III regulates cytosolic RNA:DNA hybrids and intracellular microRNA expression. J Biol Chem. 2015;290:7463-73 pubmed publisher
  42. Cho S, Park J, Kang Y. AGO2 and SETDB1 cooperate in promoter-targeted transcriptional silencing of the androgen receptor gene. Nucleic Acids Res. 2014;42:13545-56 pubmed publisher
  43. Pohlers M, Calabrese J, Magnuson T. Small RNA expression from the human macrosatellite DXZ4. G3 (Bethesda). 2014;4:1981-9 pubmed publisher
  44. Le Guillou S, Marthey S, Laloé D, Laubier J, Mobuchon L, Leroux C, et al. Characterisation and comparison of lactating mouse and bovine mammary gland miRNomes. PLoS ONE. 2014;9:e91938 pubmed publisher
  45. Boudoukha S, Rivera Vargas T, Dang I, Kropp J, Cuvellier S, Gautreau A, et al. MiRNA let-7g regulates skeletal myoblast motility via Pinch-2. FEBS Lett. 2014;588:1623-9 pubmed publisher
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