This is a Validated Antibody Database (VAD) review about human AF6, based on 10 published articles (read how Labome selects the articles), using AF6 antibody in all methods. It is aimed to help Labome visitors find the most suited AF6 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
AF6 synonym: AF6; MLL-AF6; MLLT4; l-afadin; afadin; ALL1-fused gene from chromosome 6 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4; protein AF-6

Knockout validation
Sigma-Aldrich
rabbit polyclonal
  • western blot knockout validation; human; 1:2000; fig s1
Sigma-Aldrich AF6 antibody (Sigma, A0349) was used in western blot knockout validation on human samples at 1:2000 (fig s1). Proc Natl Acad Sci U S A (2015) ncbi
Abcam
mouse monoclonal (3)
  • immunohistochemistry - frozen section; rat; 2 ug/ml; fig 6
  • immunoprecipitation; rat; 1 ug/ml; fig 6
  • western blot; rat; 0.2 ug/ml; fig 6
In order to study the increase of nectin-3 in the cell junctions of the uterine epithelium at implantation, Abcam AF6 antibody (Abcam, ab90809) was used in immunohistochemistry - frozen section on rat samples at 2 ug/ml (fig 6), in immunoprecipitation on rat samples at 1 ug/ml (fig 6) and in western blot on rat samples at 0.2 ug/ml (fig 6). Reprod Sci (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:200
In order to identify the functional tight junction barrier in human epidermis, Abcam AF6 antibody (Abcam, ab11337) was used in immunohistochemistry - frozen section on human samples at 1:200. J Dermatol Sci (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (35)
  • immunocytochemistry; human
  • immunohistochemistry; human
  • western blot; human
Santa Cruz Biotechnology AF6 antibody (Santa Cruz Biotechnology, sc-135823) was used in immunocytochemistry on human samples , in immunohistochemistry on human samples and in western blot on human samples . Biochim Biophys Acta (2014) ncbi
BD Biosciences
mouse monoclonal (35/AF6)
  • western blot; human; 1:1000; loading ...; fig 2f
BD Biosciences AF6 antibody (BD, 610732) was used in western blot on human samples at 1:1000 (fig 2f). elife (2019) ncbi
mouse monoclonal (35/AF6)
  • western blot; human
In order to investigate the role of lipolysis stimulated lipoprotein receptor in aggressive breast cancer behavior, BD Biosciences AF6 antibody (BD Transduciton Labs, 610732) was used in western blot on human samples . PLoS ONE (2014) ncbi
Sigma-Aldrich
rabbit polyclonal
  • immunocytochemistry; dog; 1:200; loading ...; fig s6b
Sigma-Aldrich AF6 antibody (Sigma-Aldrich, A0349) was used in immunocytochemistry on dog samples at 1:200 (fig s6b). Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:600; fig 4
  • western blot; mouse; 1:4000; fig 4
In order to study recruitment of PDZD11 to adherens junctions to stabilize nectins via PLEKHA7, Sigma-Aldrich AF6 antibody (Sigma, A0224) was used in immunocytochemistry on mouse samples at 1:600 (fig 4) and in western blot on mouse samples at 1:4000 (fig 4). J Biol Chem (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; fig 2
  • western blot; mouse; fig 2
In order to characterize prolactin receptor signaling for mammary gland development by a novel nectin-mediated cell adhesion apparatus, Sigma-Aldrich AF6 antibody (Sigma, A0349) was used in immunocytochemistry on mouse samples (fig 2) and in western blot on mouse samples (fig 2). J Biol Chem (2016) ncbi
rabbit polyclonal
  • western blot knockout validation; human; 1:2000; fig s1
Sigma-Aldrich AF6 antibody (Sigma, A0349) was used in western blot knockout validation on human samples at 1:2000 (fig s1). Proc Natl Acad Sci U S A (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100
In order to investigate the role of afadin in epithelial tissue development using epithelium-specific afadin-deficient mice, Sigma-Aldrich AF6 antibody (Sigma, A 0224) was used in immunohistochemistry - paraffin section on mouse samples at 1:100. Genes Cells (2014) ncbi
Articles Reviewed
  1. Fearnley G, Young K, Edgar J, Antrobus R, Hay I, Liang W, et al. The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion. elife. 2019;8: pubmed publisher
  2. Hart K, Tan J, Siemers K, Sim J, PRUITT B, Nelson W, et al. E-cadherin and LGN align epithelial cell divisions with tissue tension independently of cell shape. Proc Natl Acad Sci U S A. 2017;114:E5845-E5853 pubmed publisher
  3. Poon C, Madawala R, Dowland S, Murphy C. Nectin-3 Is Increased in the Cell Junctions of the Uterine Epithelium at Implantation. Reprod Sci. 2016;23:1580-1592 pubmed
  4. Guerrera D, Shah J, Vasileva E, Sluysmans S, Méan I, Jond L, et al. PLEKHA7 Recruits PDZD11 to Adherens Junctions to Stabilize Nectins. J Biol Chem. 2016;291:11016-29 pubmed publisher
  5. Kitayama M, Mizutani K, Maruoka M, Mandai K, Sakakibara S, Ueda Y, et al. A Novel Nectin-mediated Cell Adhesion Apparatus That Is Implicated in Prolactin Receptor Signaling for Mammary Gland Development. J Biol Chem. 2016;291:5817-31 pubmed publisher
  6. Popov L, Marceau C, Starkl P, Lumb J, Shah J, Guerrera D, et al. The adherens junctions control susceptibility to Staphylococcus aureus α-toxin. Proc Natl Acad Sci U S A. 2015;112:14337-42 pubmed publisher
  7. Yoshida T, Iwata T, Takai Y, Birchmeier W, Yamato M, Okano T. Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice. Genes Cells. 2014;19:842-52 pubmed publisher
  8. Reaves D, Fagan Solis K, Dunphy K, Oliver S, Scott D, Fleming J. The role of lipolysis stimulated lipoprotein receptor in breast cancer and directing breast cancer cell behavior. PLoS ONE. 2014;9:e91747 pubmed publisher
  9. Sun T, Wang Y, Cheng H, Xiao H, Xiang J, Zhang J, et al. Disrupted interaction between CFTR and AF-6/afadin aggravates malignant phenotypes of colon cancer. Biochim Biophys Acta. 2014;1843:618-28 pubmed
  10. Yoshida K, Yokouchi M, Nagao K, Ishii K, Amagai M, Kubo A. Functional tight junction barrier localizes in the second layer of the stratum granulosum of human epidermis. J Dermatol Sci. 2013;71:89-99 pubmed publisher