This is a Validated Antibody Database (VAD) review about human ACTR3, based on 19 published articles (read how Labome selects the articles), using ACTR3 antibody in all methods. It is aimed to help Labome visitors find the most suited ACTR3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ACTR3 synonym: ARP3; actin-related protein 3; actin-like protein 3

Abcam
rabbit monoclonal
  • immunocytochemistry; mouse; loading ...; fig 1n
  • western blot; mouse; loading ...; fig 1j
Abcam ACTR3 antibody (Abcam, ab151729) was used in immunocytochemistry on mouse samples (fig 1n) and in western blot on mouse samples (fig 1j). Dev Cell (2018) ncbi
rabbit monoclonal (EPR10429)
  • western blot; mouse; 1:1000; loading ...; fig 6c
Abcam ACTR3 antibody (Abcam, ab181164) was used in western blot on mouse samples at 1:1000 (fig 6c). Nat Commun (2018) ncbi
Invitrogen
rabbit polyclonal
  • immunocytochemistry; human; 1:250; loading ...; fig 4a
In order to investigate the connection between the centrosome and actin filaments, Invitrogen ACTR3 antibody (ThermoFisher, PA517288) was used in immunocytochemistry on human samples at 1:250 (fig 4a). Nat Cell Biol (2016) ncbi
rabbit polyclonal
  • western blot; cow; 1:1000
In order to use 2D-DIGE and mass spectrometry to identify the protein changes in mammary epithelial cells, Invitrogen ACTR3 antibody (Thermo Scientific, PA5-30354) was used in western blot on cow samples at 1:1000. PLoS ONE (2014) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; loading ...; fig 10e
In order to investigate the role of CD73 in endometrial cancer, Cell Signaling Technology ACTR3 antibody (Cell Signaling, 4738) was used in western blot on human samples (fig 10e). J Clin Invest (2016) ncbi
Sigma-Aldrich
mouse monoclonal (FMS338)
  • immunocytochemistry; human; loading ...; fig 1d
Sigma-Aldrich ACTR3 antibody (Sigma-Aldrich, A5979) was used in immunocytochemistry on human samples (fig 1d). Cell (2018) ncbi
mouse monoclonal (FMS338)
  • western blot; rat; 1:3000; loading ...; fig 3a
In order to explore how laminin2 in the basement membrane modulates the blood testis barrier dynamics during spermatogenesis, Sigma-Aldrich ACTR3 antibody (Sigma, A5979) was used in western blot on rat samples at 1:3000 (fig 3a). FASEB J (2017) ncbi
mouse monoclonal (FMS338)
  • immunocytochemistry; human; loading ...; fig 3d
Sigma-Aldrich ACTR3 antibody (Sigma-Aldrich, FMS338) was used in immunocytochemistry on human samples (fig 3d). Oncogene (2017) ncbi
mouse monoclonal (FMS338)
  • immunocytochemistry; rat; 1:100; loading ...; tbl 1
  • western blot; rat; 1:3000; loading ...; tbl 1
In order to examine the organization of actin microfilaments in Sertoli cell at the blood testis barrier, Sigma-Aldrich ACTR3 antibody (Sigma, A5979) was used in immunocytochemistry on rat samples at 1:100 (tbl 1) and in western blot on rat samples at 1:3000 (tbl 1). Spermatogenesis (2016) ncbi
mouse monoclonal (FMS338)
  • immunocytochemistry; rat; 1:100; fig 5
  • western blot; rat; 1:3000; fig 1
In order to characterize overexpression of gap junction protein connexin 43 by rescue of perfluorooctanesulfonate (PFOS)-mediated sertoli cell injury, Sigma-Aldrich ACTR3 antibody (Sigma, A5979) was used in immunocytochemistry on rat samples at 1:100 (fig 5) and in western blot on rat samples at 1:3000 (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (FMS338)
  • immunoprecipitation; rat; fig 4
  • immunocytochemistry; rat; 1:50; fig 6
  • western blot; rat; 1:3000; fig 5
In order to report that crumbs homolog 3 is an actin microfilament regulator, Sigma-Aldrich ACTR3 antibody (Sigma-Aldrich, A5979) was used in immunoprecipitation on rat samples (fig 4), in immunocytochemistry on rat samples at 1:50 (fig 6) and in western blot on rat samples at 1:3000 (fig 5). Sci Rep (2016) ncbi
mouse monoclonal (FMS338)
  • proximity ligation assay; human; 1:200; fig 5
  • immunocytochemistry; human; 1:200; fig 7
  • western blot; human; 1:400
In order to maintain adherens junction stability and tension by neogenin recruitment of the WAVE regulatory complex, Sigma-Aldrich ACTR3 antibody (Sigma-Aldrich, A5979) was used in proximity ligation assay on human samples at 1:200 (fig 5), in immunocytochemistry on human samples at 1:200 (fig 7) and in western blot on human samples at 1:400. Nat Commun (2016) ncbi
mouse monoclonal (FMS338)
  • immunohistochemistry - paraffin section; rat; 1:3000; fig 2
  • immunoprecipitation; rat; 1:250; fig 2
  • immunocytochemistry; rat; 1:50; fig 8
  • immunohistochemistry; rat; 1:200; fig 2
In order to determine regulation of ectoplasmic specialization dynamics via its effects on actin microfilaments in the testes of male rats by planar cell polarity (PCP) protein Vangl2, Sigma-Aldrich ACTR3 antibody (Sigma, A5979) was used in immunohistochemistry - paraffin section on rat samples at 1:3000 (fig 2), in immunoprecipitation on rat samples at 1:250 (fig 2), in immunocytochemistry on rat samples at 1:50 (fig 8) and in immunohistochemistry on rat samples at 1:200 (fig 2). Endocrinology (2016) ncbi
mouse monoclonal (FMS338)
  • western blot; mouse; 1:200; fig s7
Sigma-Aldrich ACTR3 antibody (Sigma, A5979-200UL) was used in western blot on mouse samples at 1:200 (fig s7). Nat Commun (2016) ncbi
mouse monoclonal (FMS338)
  • immunohistochemistry - frozen section; rat; 1:200; fig 3
In order to study transport of spermatids and residual bodies/phagosomes during spermatogenesis by coordination of microtuble-and actin-based cytoskeletons in rat testis, Sigma-Aldrich ACTR3 antibody (Sigma-Aldrich, A5979) was used in immunohistochemistry - frozen section on rat samples at 1:200 (fig 3). Endocrinology (2016) ncbi
mouse monoclonal (FMS338)
  • western blot; human; 1:1000; loading ...; fig 1c
Sigma-Aldrich ACTR3 antibody (Sigma, A5979) was used in western blot on human samples at 1:1000 (fig 1c). Nat Cell Biol (2016) ncbi
mouse monoclonal (FMS338)
  • immunohistochemistry; rat; 1:50
  • western blot; rat; 1:3000
In order to study the role of plastin 3 in ectoplasmic specialization dynamics during spermatogenesis in the rat testis, Sigma-Aldrich ACTR3 antibody (Sigma-Aldrich, A5979) was used in immunohistochemistry on rat samples at 1:50 and in western blot on rat samples at 1:3000. FASEB J (2015) ncbi
mouse monoclonal (FMS338)
  • immunohistochemistry - frozen section; rat; 1:200
  • western blot; rat; 1:3000
In order to study the role of EB1 in tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier, Sigma-Aldrich ACTR3 antibody (Sigma-Aldrich, A5979) was used in immunohistochemistry - frozen section on rat samples at 1:200 and in western blot on rat samples at 1:3000. Endocrinology (2015) ncbi
mouse monoclonal (FMS338)
  • immunocytochemistry; human
In order to investigate the role of the actin cytoskeleton of the endothelium in transmigration, Sigma-Aldrich ACTR3 antibody (Sigma-Aldrich, FMS338) was used in immunocytochemistry on human samples . Mol Biol Cell (2014) ncbi
Articles Reviewed
  1. Schell C, Sabass B, Helmstaedter M, Geist F, Abed A, Yasuda Yamahara M, et al. ARP3 Controls the Podocyte Architecture at the Kidney Filtration Barrier. Dev Cell. 2018;47:741-757.e8 pubmed publisher
  2. Kim H, Mun Y, Lee K, Park Y, Park J, Park J, et al. T cell microvilli constitute immunological synaptosomes that carry messages to antigen-presenting cells. Nat Commun. 2018;9:3630 pubmed publisher
  3. Almeida Souza L, Frank R, García Nafría J, Colussi A, Gunawardana N, Johnson C, et al. A Flat BAR Protein Promotes Actin Polymerization at the Base of Clathrin-Coated Pits. Cell. 2018;174:325-337.e14 pubmed publisher
  4. Gao Y, Mruk D, Chen H, Lui W, Lee W, Cheng C. Regulation of the blood-testis barrier by a local axis in the testis: role of laminin ?2 in the basement membrane. FASEB J. 2017;31:584-597 pubmed publisher
  5. Frank S, Köllmann C, van Lidth de Jeude J, Thiagarajah J, Engelholm L, Frödin M, et al. The focal adhesion-associated proteins DOCK5 and GIT2 comprise a rheostat in control of epithelial invasion. Oncogene. 2017;36:1816-1828 pubmed publisher
  6. Li N, Lee W, Cheng C. Overexpression of plastin 3 in Sertoli cells disrupts actin microfilament bundle homeostasis and perturbs the tight junction barrier. Spermatogenesis. 2016;6:e1206353 pubmed publisher
  7. Li N, Mruk D, Chen H, Wong C, Lee W, Cheng C. Rescue of perfluorooctanesulfonate (PFOS)-mediated Sertoli cell injury by overexpression of gap junction protein connexin 43. Sci Rep. 2016;6:29667 pubmed publisher
  8. Gao Y, Lui W, Lee W, Cheng C. Polarity protein Crumbs homolog-3 (CRB3) regulates ectoplasmic specialization dynamics through its action on F-actin organization in Sertoli cells. Sci Rep. 2016;6:28589 pubmed publisher
  9. Lee N, Fok K, White A, Wilson N, O Leary C, Cox H, et al. Neogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension. Nat Commun. 2016;7:11082 pubmed publisher
  10. Chen H, Mruk D, Lee W, Cheng C. Planar Cell Polarity (PCP) Protein Vangl2 Regulates Ectoplasmic Specialization Dynamics via Its Effects on Actin Microfilaments in the Testes of Male Rats. Endocrinology. 2016;157:2140-59 pubmed publisher
  11. Obino D, Farina F, Malbec O, Sáez P, Maurin M, Gaillard J, et al. Actin nucleation at the centrosome controls lymphocyte polarity. Nat Commun. 2016;7:10969 pubmed publisher
  12. Tang E, Lee W, Cheng C. Coordination of Actin- and Microtubule-Based Cytoskeletons Supports Transport of Spermatids and Residual Bodies/Phagosomes During Spermatogenesis in the Rat Testis. Endocrinology. 2016;157:1644-59 pubmed publisher
  13. Abella J, Galloni C, Pernier J, Barry D, Kjær S, Carlier M, et al. Isoform diversity in the Arp2/3 complex determines actin filament dynamics. Nat Cell Biol. 2016;18:76-86 pubmed publisher
  14. Farina F, Gaillard J, Guérin C, Couté Y, Sillibourne J, Blanchoin L, et al. The centrosome is an actin-organizing centre. Nat Cell Biol. 2016;18:65-75 pubmed publisher
  15. Bowser J, Blackburn M, Shipley G, Molina J, Dunner K, Broaddus R. Loss of CD73-mediated actin polymerization promotes endometrial tumor progression. J Clin Invest. 2016;126:220-38 pubmed publisher
  16. Li N, Mruk D, Wong C, Lee W, Han D, Cheng C. Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis. FASEB J. 2015;29:3788-805 pubmed publisher
  17. Tang E, Mok K, Lee W, Cheng C. EB1 regulates tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier in male rats: an in vitro study. Endocrinology. 2015;156:680-93 pubmed publisher
  18. Mooren O, Li J, Nawas J, Cooper J. Endothelial cells use dynamic actin to facilitate lymphocyte transendothelial migration and maintain the monolayer barrier. Mol Biol Cell. 2014;25:4115-29 pubmed publisher
  19. Janjanam J, Singh S, Jena M, Varshney N, Kola S, Kumar S, et al. Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield. PLoS ONE. 2014;9:e102515 pubmed publisher