This is a Validated Antibody Database (VAD) review about human ACTH, based on 29 published articles (read how Labome selects the articles), using ACTH antibody in all methods. It is aimed to help Labome visitors find the most suited ACTH antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ACTH synonym: ACTH; CLIP; LPH; MSH; NPP; OBAIRH; POC; pro-opiomelanocortin; adrenocorticotropic hormone; adrenocorticotropin; alpha-MSH; alpha-melanocyte-stimulating hormone; beta-LPH; beta-MSH; beta-endorphin; beta-melanocyte-stimulating hormone; corticotropin-like intermediary peptide; corticotropin-lipotropin; gamma-LPH; gamma-MSH; lipotropin beta; lipotropin gamma; melanotropin alpha; melanotropin beta; melanotropin gamma; met-enkephalin; opiomelanocortin prepropeptide; pro-ACTH-endorphin; proopiomelanocortin preproprotein

Knockout validation
Phoenix Pharmaceuticals
rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:3000; loading ...; fig 4d
In order to investigate the role of the ARC to PVH satiety circuit in hunger and satiety, Phoenix Pharmaceuticals ACTH antibody (Phoenix Pharmaceuticals, H-029-30) was used in immunohistochemistry knockout validation on mouse samples at 1:3000 (fig 4d). Nat Neurosci (2017) ncbi
Santa Cruz Biotechnology
mouse monoclonal (SPM333)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig st1
  • immunohistochemistry - paraffin section; rat; 1:100; loading ...; fig st1
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig st1
In order to outline the protocols for antibodies used for immunohistochemical studies, Santa Cruz Biotechnology ACTH antibody (Santa, sc-52980) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig st1), in immunohistochemistry - paraffin section on rat samples at 1:100 (fig st1) and in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig st1). J Toxicol Pathol (2017) ncbi
mouse monoclonal (SPM333)
  • western blot; rat; 1:1000; loading ...; fig 1c
In order to study the effect of moderate exercise on anterior pituitary gland in diet-induced insulin resistance, Santa Cruz Biotechnology ACTH antibody (SCBT, sc-52980) was used in western blot on rat samples at 1:1000 (fig 1c). Endocrinology (2016) ncbi
mouse monoclonal (B427)
  • immunohistochemistry - paraffin section; human; fig 1
In order to examine the contribution of thyroid hormone beta to TSH-secreting adenomas, Santa Cruz Biotechnology ACTH antibody (santa cruz, sc-57 021) was used in immunohistochemistry - paraffin section on human samples (fig 1). J Clin Endocrinol Metab (2015) ncbi
mouse monoclonal (NOC1/35)
  • immunohistochemistry; mouse; 1:50; fig 5
Santa Cruz Biotechnology ACTH antibody (Santa Cruz, sc-47705) was used in immunohistochemistry on mouse samples at 1:50 (fig 5). PLoS ONE (2013) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry - free floating section; rat; 1:1000; loading ...; fig 1a
In order to characterize Kiss1r-positive neurons in the arcuate nucleus of the rat hypothalamus, Abcam ACTH antibody (Abcam, ab94446) was used in immunohistochemistry - free floating section on rat samples at 1:1000 (fig 1a). J Neuroendocrinol (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 3
Abcam ACTH antibody (Abcam, ab74976) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 3). Oncol Lett (2016) ncbi
goat polyclonal
  • immunohistochemistry; mouse; 1:100; fig 4
Abcam ACTH antibody (Abcam, ab322893) was used in immunohistochemistry on mouse samples at 1:100 (fig 4). Neural Dev (2016) ncbi
goat polyclonal
  • western blot; mouse; 1:2000; fig 4
Abcam ACTH antibody (abcam, ab32893) was used in western blot on mouse samples at 1:2000 (fig 4). Nat Commun (2016) ncbi
ImmunoStar
rabbit polyclonal
  • immunohistochemistry; mouse; 1:2000; fig s1
ImmunoStar ACTH antibody (Immunostar, 20063) was used in immunohistochemistry on mouse samples at 1:2000 (fig s1). Gastroenterology (2014) ncbi
Invitrogen
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1b
Invitrogen ACTH antibody (Thermo Fisher, PA1-36035) was used in immunohistochemistry on mouse samples at 1:200 (fig 1b). Science (2018) ncbi
Phoenix Pharmaceuticals
rabbit polyclonal
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 7b
In order to develop methods to identify the major projections to the mesolimbic dopamine circuit from the lateral hypothalamus and dorsal raphe nucleus, Phoenix Pharmaceuticals ACTH antibody (Phoenix Pharmaceuticals, H-029-30) was used in immunohistochemistry on mouse samples at 1:1000 (fig 7b). J Neurosci (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 2b
In order to determine the effects of dorsomedial hypothalamus cholinergic neurons in regulating food intake, Phoenix Pharmaceuticals ACTH antibody (Phoenix pharmaceuticals, H-029-30) was used in immunohistochemistry on mouse samples at 1:1000 (fig 2b). Mol Metab (2017) ncbi
rabbit polyclonal
  • immunohistochemistry knockout validation; mouse; 1:3000; loading ...; fig 4d
In order to investigate the role of the ARC to PVH satiety circuit in hunger and satiety, Phoenix Pharmaceuticals ACTH antibody (Phoenix Pharmaceuticals, H-029-30) was used in immunohistochemistry knockout validation on mouse samples at 1:3000 (fig 4d). Nat Neurosci (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:20,000; loading ...; fig 8
In order to investigate non-neuronal proopiomelanocortin expression, Phoenix Pharmaceuticals ACTH antibody (pheonix, H-029-30) was used in immunohistochemistry on rat samples at 1:20,000 (fig 8). J Comp Neurol (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; fig 4
Phoenix Pharmaceuticals ACTH antibody (Phoenix Pharmaceuticals, H-029-30) was used in immunohistochemistry on mouse samples (fig 4). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; rat; 1:2000; fig 6
In order to study the anatomy of the satiety network by focusing on connectivity of the parabrachial nucleus in the adult rat, Phoenix Pharmaceuticals ACTH antibody (Phoenix Pharmaceuticals, H-029-30) was used in immunohistochemistry - frozen section on rat samples at 1:2000 (fig 6). J Comp Neurol (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; rat; 1:1000; loading ...; fig 2d
In order to examine the involvement of MC3R in the modulation of food intake, Phoenix Pharmaceuticals ACTH antibody (Phoenix Pharmapseuticals, H-029-30) was used in immunohistochemistry - free floating section on rat samples at 1:1000 (fig 2d). Neuropsychopharmacology (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; rat
Phoenix Pharmaceuticals ACTH antibody (Phoenix Pharmapseuticals, H-029-30) was used in immunohistochemistry - free floating section on rat samples . PLoS ONE (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:2000; fig 6
Phoenix Pharmaceuticals ACTH antibody (Phoenix Pharmaceuticals, H02930) was used in immunohistochemistry on mouse samples at 1:2000 (fig 6). Nat Neurosci (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; rat; 1:1000
  • western blot; rat
Phoenix Pharmaceuticals ACTH antibody (Phoenix Pharmaceuticals, H-029-30) was used in immunocytochemistry on rat samples at 1:1000 and in western blot on rat samples . J Comp Neurol (2015) ncbi
Dako
mouse monoclonal (02A3)
  • immunohistochemistry - paraffin section; rat; 1:500; loading ...; fig st2
In order to outline the protocols for antibodies used for immunohistochemical studies, Dako ACTH antibody (Dako, M3501) was used in immunohistochemistry - paraffin section on rat samples at 1:500 (fig st2). J Toxicol Pathol (2017) ncbi
mouse monoclonal (02A3)
  • immunohistochemistry; human; loading ...
In order to determine if the isocitrate dehydrogenase R132H mutation is present in pituitary adenomas, Dako ACTH antibody (DAKO, 02A3) was used in immunohistochemistry on human samples . Pituitary (2016) ncbi
mouse monoclonal (02A3)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...
In order to report the expression of senescence-associated lysosomal beta-galactosidase, p16, and p21 in different types of pituitary adenomas, Dako ACTH antibody (DAKO, 02A3) was used in immunohistochemistry - paraffin section on human samples at 1:100. Endocr Pathol (2016) ncbi
mouse monoclonal (02A3)
  • immunohistochemistry - paraffin section; human; fig 2
In order to describe the immune cell infiltrates found in pituitary adenomas, Dako ACTH antibody (Dako, O2A3) was used in immunohistochemistry - paraffin section on human samples (fig 2). Endocr Pathol (2015) ncbi
mouse monoclonal (02A3)
  • immunohistochemistry - paraffin section; mouse; fig 3
Dako ACTH antibody (Dako, M3501) was used in immunohistochemistry - paraffin section on mouse samples (fig 3). PLoS ONE (2015) ncbi
mouse monoclonal (02A3)
  • immunohistochemistry - free floating section; mouse; 1:500
Dako ACTH antibody (Dako, M3501) was used in immunohistochemistry - free floating section on mouse samples at 1:500. J Comp Neurol (2012) ncbi
EMD Millipore
mouse monoclonal (3-E7)
  • blocking or activating experiments; mouse; loading ...
In order to study to role of Sigma-1 receptors in peripheral opioid analgesia during inflammation, EMD Millipore ACTH antibody (Millipore, MAB5276) was used in blocking or activating experiments on mouse samples . Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:2000; fig s2c
In order to investigate the role of hypothalamus in regulating local adult neural stem cells and neurogenesis, EMD Millipore ACTH antibody (Millipore, AB5028) was used in immunohistochemistry on mouse samples at 1:2000 (fig s2c). Science (2017) ncbi
mouse monoclonal (3-E7)
  • immunohistochemistry; mouse; 1:500; fig s2a
In order to investigate the role of hypothalamus in regulating local adult neural stem cells and neurogenesis, EMD Millipore ACTH antibody (Millipore, MAB5276) was used in immunohistochemistry on mouse samples at 1:500 (fig s2a). Science (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:1000
EMD Millipore ACTH antibody (Chemicon [Millipore], AB5028) was used in immunohistochemistry - frozen section on human samples at 1:1000. Neuroscience (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; axolotl; 1:30000
EMD Millipore ACTH antibody (Chemicon, AB902) was used in immunohistochemistry - frozen section on axolotl samples at 1:30000. Tissue Cell (2014) ncbi
Biosensis
guinea pigs polyclonal
  • immunohistochemistry - free floating section; cow; 1:40000
Biosensis ACTH antibody (Biosensis, cat#GP-030-50) was used in immunohistochemistry - free floating section on cow samples at 1:40000. J Neuroendocrinol (2015) ncbi
Articles Reviewed
  1. Luo S, Huang J, Li Q, Mohammad H, Lee C, Krishna K, et al. Regulation of feeding by somatostatin neurons in the tuberal nucleus. Science. 2018;361:76-81 pubmed publisher
  2. Tejada M, Montilla García Á, Cronin S, Cikes D, Sánchez Fernández C, González Cano R, et al. Sigma-1 receptors control immune-driven peripheral opioid analgesia during inflammation in mice. Proc Natl Acad Sci U S A. 2017;114:8396-8401 pubmed publisher
  3. Paul A, Chaker Z, Doetsch F. Hypothalamic regulation of regionally distinct adult neural stem cells and neurogenesis. Science. 2017;356:1383-1386 pubmed publisher
  4. Pomeranz L, Ekstrand M, Latcha K, Smith G, Enquist L, Friedman J. Gene Expression Profiling with Cre-Conditional Pseudorabies Virus Reveals a Subset of Midbrain Neurons That Participate in Reward Circuitry. J Neurosci. 2017;37:4128-4144 pubmed publisher
  5. Jeong J, Lee D, Jo Y. Cholinergic neurons in the dorsomedial hypothalamus regulate food intake. Mol Metab. 2017;6:306-312 pubmed publisher
  6. Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
  7. Higo S, Iijima N, Ozawa H. Characterisation of Kiss1r (Gpr54)-Expressing Neurones in the Arcuate Nucleus of the Female Rat Hypothalamus. J Neuroendocrinol. 2017;29: pubmed publisher
  8. Fenselau H, Campbell J, Verstegen A, Madara J, Xu J, Shah B, et al. A rapidly acting glutamatergic ARC?PVH satiety circuit postsynaptically regulated by ?-MSH. Nat Neurosci. 2017;20:42-51 pubmed publisher
  9. Wittmann G, Farkas E, Szilvásy Szabó A, Gereben B, Fekete C, Lechan R. Variable proopiomelanocortin expression in tanycytes of the adult rat hypothalamus and pituitary stalk. J Comp Neurol. 2017;525:411-441 pubmed publisher
  10. Pu J, Wang Z, Zhou H, Zhong A, Jin K, Ruan L, et al. Isolated double adrenocorticotropic hormone-secreting pituitary adenomas: A case report and review of the literature. Oncol Lett. 2016;12:585-590 pubmed
  11. Sokolowski K, Tran T, Esumi S, Kamal Y, Oboti L, Lischinsky J, et al. Molecular and behavioral profiling of Dbx1-derived neurons in the arcuate, lateral and ventromedial hypothalamic nuclei. Neural Dev. 2016;11:12 pubmed publisher
  12. Casar Borota O, Øystese K, Sundstrom M, Melchior L, Popovic V. A high-throughput analysis of the IDH1(R132H) protein expression in pituitary adenomas. Pituitary. 2016;19:407-14 pubmed publisher
  13. Kabra D, Pfuhlmann K, García Cáceres C, Schriever S, Casquero García V, Kebede A, et al. Hypothalamic leptin action is mediated by histone deacetylase 5. Nat Commun. 2016;7:10782 pubmed publisher
  14. Zséli G, Vida B, Martinez A, Lechan R, Khan A, Fekete C. Elucidation of the anatomy of a satiety network: Focus on connectivity of the parabrachial nucleus in the adult rat. J Comp Neurol. 2016;524:2803-27 pubmed publisher
  15. Pandit R, Omrani A, Luijendijk M, de Vrind V, van Rozen A, Ophuis R, et al. Melanocortin 3 Receptor Signaling in Midbrain Dopamine Neurons Increases the Motivation for Food Reward. Neuropsychopharmacology. 2016;41:2241-51 pubmed publisher
  16. Mercau M, Repetto E, Perez M, Martinez Calejman C, Sánchez Puch S, Finkielstein C, et al. Moderate Exercise Prevents Functional Remodeling of the Anterior Pituitary Gland in Diet-Induced Insulin Resistance in Rats: Role of Oxidative Stress and Autophagy. Endocrinology. 2016;157:1135-45 pubmed publisher
  17. Manojlović Gacić E, Skender Gazibara M, Popovic V, Soldatovic I, Boricic N, Raičević S, et al. Oncogene-Induced Senescence in Pituitary Adenomas--an Immunohistochemical Study. Endocr Pathol. 2016;27:1-11 pubmed publisher
  18. Lu J, Adam B, Jack A, Lam A, Broad R, Chik C. Immune Cell Infiltrates in Pituitary Adenomas: More Macrophages in Larger Adenomas and More T Cells in Growth Hormone Adenomas. Endocr Pathol. 2015;26:263-72 pubmed publisher
  19. Cardoso R, Alves B, Sharpton S, Williams G, Amstalden M. Nutritional Programming of Accelerated Puberty in Heifers: Involvement of Pro-Opiomelanocortin Neurones in the Arcuate Nucleus. J Neuroendocrinol. 2015;27:647-57 pubmed publisher
  20. Teng X, Jin T, Brent G, Wu A, Teng W, Shan Z. A Patient With a Thyrotropin-Secreting Microadenoma and Resistance to Thyroid Hormone (P453T). J Clin Endocrinol Metab. 2015;100:2511-4 pubmed publisher
  21. Pandit R, van der Zwaal E, Luijendijk M, Brans M, van Rozen A, Oude Ophuis R, et al. Central melanocortins regulate the motivation for sucrose reward. PLoS ONE. 2015;10:e0121768 pubmed publisher
  22. O Hara L, Curley M, Tedim Ferreira M, Cruickshanks L, Milne L, Smith L. Pituitary androgen receptor signalling regulates prolactin but not gonadotrophins in the male mouse. PLoS ONE. 2015;10:e0121657 pubmed publisher
  23. Atasoy D, Betley J, Li W, Su H, Sertel S, Scheffer L, et al. A genetically specified connectomics approach applied to long-range feeding regulatory circuits. Nat Neurosci. 2014;17:1830-9 pubmed publisher
  24. Caron A, Baraboi E, Laplante M, Richard D. DEP domain-containing mTOR-interacting protein in the rat brain: distribution of expression and potential implication. J Comp Neurol. 2015;523:93-107 pubmed publisher
  25. Shen H, Canas P, García Sanz P, Lan J, Boison D, Moratalla R, et al. Adenosine A?A receptors in striatal glutamatergic terminals and GABAergic neurons oppositely modulate psychostimulant action and DARPP-32 phosphorylation. PLoS ONE. 2013;8:e80902 pubmed publisher
  26. Olsen J, Peroski M, Kiczek M, Grignol G, Merchenthaler I, Dudas B. Intimate associations between the endogenous opiate systems and the growth hormone-releasing hormone system in the human hypothalamus. Neuroscience. 2014;258:238-45 pubmed publisher
  27. Suzuki H, Yamamoto T. Leucine-enkephalin-like immunoreactivity is localized in luteinizing hormone-producing cells in the axolotl (Ambystoma mexicanum) pituitary. Tissue Cell. 2014;46:15-20 pubmed publisher
  28. DelGiorno K, Hall J, Takeuchi K, Pan F, Halbrook C, Washington M, et al. Identification and manipulation of biliary metaplasia in pancreatic tumors. Gastroenterology. 2014;146:233-44.e5 pubmed publisher
  29. Kühne C, Puk O, Graw J, Hrabe de Angelis M, Schutz G, Wurst W, et al. Visualizing corticotropin-releasing hormone receptor type 1 expression and neuronal connectivities in the mouse using a novel multifunctional allele. J Comp Neurol. 2012;520:3150-80 pubmed publisher