This is a Validated Antibody Database (VAD) review about human ABHD5, based on 16 published articles (read how Labome selects the articles), using ABHD5 antibody in all methods. It is aimed to help Labome visitors find the most suited ABHD5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ABHD5 synonym: CGI58; IECN2; NCIE2

Knockout validation
Abnova
mouse monoclonal (1F3)
  • western blot knockout validation; human; loading ...; fig 4b
Abnova ABHD5 antibody (Abnova, H00051099-M01) was used in western blot knockout validation on human samples (fig 4b). Sci Rep (2020) ncbi
Abnova
mouse monoclonal (1F3)
  • western blot knockout validation; human; fig 1k
  • immunoprecipitation; human; fig 5b
  • immunocytochemistry; human; fig 5a
  • immunohistochemistry; human; loading ...; fig 8a
In order to propose that ABHD5 has a PNPLA2-independent function in regulating autophagy and tumorigenesis, Abnova ABHD5 antibody (Abnova, H00051099-M01) was used in western blot knockout validation on human samples (fig 1k), in immunoprecipitation on human samples (fig 5b), in immunocytochemistry on human samples (fig 5a) and in immunohistochemistry on human samples (fig 8a). Autophagy (2016) ncbi
Abnova
mouse monoclonal (1F3)
  • western blot knockout validation; human; loading ...; fig 4b
Abnova ABHD5 antibody (Abnova, H00051099-M01) was used in western blot knockout validation on human samples (fig 4b). Sci Rep (2020) ncbi
mouse monoclonal (1F3)
  • western blot; human; 1:250; loading ...; fig 1d
Abnova ABHD5 antibody (Abnova, H00051099-M01) was used in western blot on human samples at 1:250 (fig 1d). Int J Obes (Lond) (2017) ncbi
mouse monoclonal (1F3)
  • western blot knockout validation; human; fig 1k
  • immunoprecipitation; human; fig 5b
  • immunocytochemistry; human; fig 5a
  • immunohistochemistry; human; loading ...; fig 8a
In order to propose that ABHD5 has a PNPLA2-independent function in regulating autophagy and tumorigenesis, Abnova ABHD5 antibody (Abnova, H00051099-M01) was used in western blot knockout validation on human samples (fig 1k), in immunoprecipitation on human samples (fig 5b), in immunocytochemistry on human samples (fig 5a) and in immunohistochemistry on human samples (fig 8a). Autophagy (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:1000; fig 3
  • immunohistochemistry - paraffin section; human; 1:1000; fig s1
Abnova ABHD5 antibody (Abnova, PAB12500) was used in immunocytochemistry on mouse samples at 1:1000 (fig 3) and in immunohistochemistry - paraffin section on human samples at 1:1000 (fig s1). Nat Commun (2016) ncbi
mouse monoclonal (1F3)
  • western blot; mouse; 1:1000; fig 1
Abnova ABHD5 antibody (Abnova, H00051099-M01) was used in western blot on mouse samples at 1:1000 (fig 1). Nat Commun (2016) ncbi
mouse monoclonal (1F3)
  • immunocytochemistry; human; 1:1000; fig s3
  • western blot; human; 1:1000; fig 2
Abnova ABHD5 antibody (Abnova, H00051099-M01) was used in immunocytochemistry on human samples at 1:1000 (fig s3) and in western blot on human samples at 1:1000 (fig 2). PLoS Pathog (2016) ncbi
mouse monoclonal (1F3)
  • immunoprecipitation; mouse; 5:700; loading ...; fig 1e
  • western blot; mouse; loading ...; fig 1e
Abnova ABHD5 antibody (Abnova, H00051099-M01) was used in immunoprecipitation on mouse samples at 5:700 (fig 1e) and in western blot on mouse samples (fig 1e). J Biol Chem (2015) ncbi
mouse monoclonal (1F3)
  • western blot; mouse; fig 1d
Abnova ABHD5 antibody (Abnova, H00051099-M01) was used in western blot on mouse samples (fig 1d). Int J Mol Sci (2015) ncbi
mouse monoclonal (1F3)
  • western blot; human
In order to study the role of skeletal muscle lipid handling in obese patients with type 2 diabetes, Abnova ABHD5 antibody (Abnova, H00051099-M01) was used in western blot on human samples . PLoS ONE (2015) ncbi
mouse monoclonal (1F3)
  • western blot; human; 1:800
Abnova ABHD5 antibody (Abnova, H00051099-M01) was used in western blot on human samples at 1:800. Diabetes (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (E-1)
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 2c
Santa Cruz Biotechnology ABHD5 antibody (Santa Cruz Biotechnolog, sc- 376931) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 2c). elife (2019) ncbi
mouse monoclonal (36A)
  • immunoprecipitation; human; fig 5b
In order to propose that ABHD5 has a PNPLA2-independent function in regulating autophagy and tumorigenesis, Santa Cruz Biotechnology ABHD5 antibody (Santa Cruz, sc-100468) was used in immunoprecipitation on human samples (fig 5b). Autophagy (2016) ncbi
mouse monoclonal (36A)
  • western blot; mouse; 1:1000; fig 1
In order to investigate the role of Fsp27 in adipose inflammation and hepatic insulin resistance, Santa Cruz Biotechnology ABHD5 antibody (Santa Cruz, sc100468) was used in western blot on mouse samples at 1:1000 (fig 1). Nat Commun (2015) ncbi
mouse monoclonal (36A)
  • western blot; mouse
Santa Cruz Biotechnology ABHD5 antibody (Santa Cruz Biotechnology, sc-100468) was used in western blot on mouse samples . Am J Physiol Endocrinol Metab (2014) ncbi
Abcam
domestic rabbit monoclonal (EPR12621)
  • western blot; human; fig 5
In order to describe the effects of long- and short-term exposure to erythropoietin on white adipose tissue, Abcam ABHD5 antibody (Abcam, ab183739) was used in western blot on human samples (fig 5). Lipids Health Dis (2016) ncbi
domestic rabbit monoclonal (EPR12621)
  • western blot; human; loading ...; fig 4c
In order to analyze pro- and anti-lipolytic signaling pathways in adipocytes after in vivo endotoxin exposure, Abcam ABHD5 antibody (Abcam, ab183739) was used in western blot on human samples (fig 4c). PLoS ONE (2016) ncbi
Novus Biologicals
domestic rabbit polyclonal
Novus Biologicals ABHD5 antibody (Novus Biologicals, NB110-41576) was used . PLoS ONE (2015) ncbi
domestic rabbit polyclonal
Novus Biologicals ABHD5 antibody (Novus Biologicals, NB110-41576) was used . Lipids (2015) ncbi
Articles Reviewed
  1. Yang P, Wang Y, Tang W, Sun W, Ma Y, Lin S, et al. Western diet induces severe nonalcoholic steatohepatitis, ductular reaction, and hepatic fibrosis in liver CGI-58 knockout mice. Sci Rep. 2020;10:4701 pubmed publisher
  2. Jung M, Wells D, Rusch J, Ahmad S, Marchini J, Myers S, et al. Unified single-cell analysis of testis gene regulation and pathology in five mouse strains. elife. 2019;8: pubmed publisher
  3. Hirschmugl B, Desoye G, Catalano P, Klymiuk I, Scharnagl H, Payr S, et al. Maternal obesity modulates intracellular lipid turnover in the human term placenta. Int J Obes (Lond). 2017;41:317-323 pubmed publisher
  4. Christensen B, Nellemann B, Jørgensen J, Pedersen S, Jessen N. Erythropoietin does not activate erythropoietin receptor signaling or lipolytic pathways in human subcutaneous white adipose tissue in vivo. Lipids Health Dis. 2016;15:160 pubmed publisher
  5. Rittig N, Bach E, Thomsen H, Pedersen S, Nielsen T, Jørgensen J, et al. Regulation of Lipolysis and Adipose Tissue Signaling during Acute Endotoxin-Induced Inflammation: A Human Randomized Crossover Trial. PLoS ONE. 2016;11:e0162167 pubmed publisher
  6. Peng Y, Miao H, Wu S, Yang W, Zhang Y, Xie G, et al. ABHD5 interacts with BECN1 to regulate autophagy and tumorigenesis of colon cancer independent of PNPLA2. Autophagy. 2016;12:2167-2182 pubmed
  7. Miao H, Ou J, Peng Y, Zhang X, Chen Y, Hao L, et al. Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM. Nat Commun. 2016;7:11716 pubmed publisher
  8. Vieyres G, Welsch K, Gerold G, Gentzsch J, Kahl S, Vondran F, et al. ABHD5/CGI-58, the Chanarin-Dorfman Syndrome Protein, Mobilises Lipid Stores for Hepatitis C Virus Production. PLoS Pathog. 2016;12:e1005568 pubmed publisher
  9. Hofer P, Boeszoermenyi A, Jaeger D, Feiler U, Arthanari H, Mayer N, et al. Fatty Acid-binding Proteins Interact with Comparative Gene Identification-58 Linking Lipolysis with Lipid Ligand Shuttling. J Biol Chem. 2015;290:18438-53 pubmed publisher
  10. Das S, Stadelmeyer E, Schauer S, Schwarz A, Strohmaier H, Claudel T, et al. Micro RNA-124a regulates lipolysis via adipose triglyceride lipase and comparative gene identification 58. Int J Mol Sci. 2015;16:8555-68 pubmed publisher
  11. Turnbull P, Ramos S, Macpherson R, Roy B, Peters S. Characterization of lipolytic inhibitor G(0)/G(1) switch gene-2 protein (G0S2) expression in male Sprague-Dawley rat skeletal muscle compared to relative content of adipose triglyceride lipase (ATGL) and comparitive gene identification-58 (CGI-58). PLoS ONE. 2015;10:e0120136 pubmed publisher
  12. Bakke S, Feng Y, Nikolić N, Kase E, Moro C, Stensrud C, et al. Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects. PLoS ONE. 2015;10:e0119556 pubmed publisher
  13. Zhou L, Park S, Xu L, Xia X, Ye J, Su L, et al. Insulin resistance and white adipose tissue inflammation are uncoupled in energetically challenged Fsp27-deficient mice. Nat Commun. 2015;6:5949 pubmed publisher
  14. Macpherson R, Castelli L, Miotto P, Frendo Cumbo S, Milburn A, Roy B, et al. A maternal high fat diet has long-lasting effects on skeletal muscle lipid and PLIN protein content in rat offspring at young adulthood. Lipids. 2015;50:205-17 pubmed publisher
  15. Lakeland T, Borg M, Matzaris M, Abdelkader A, Evans R, Watt M. Augmented expression and secretion of adipose-derived pigment epithelium-derived factor does not alter local angiogenesis or contribute to the development of systemic metabolic derangements. Am J Physiol Endocrinol Metab. 2014;306:E1367-77 pubmed publisher
  16. Kurtz C, Peck B, Fannin E, Beysen C, Miao J, Landstreet S, et al. MicroRNA-29 fine-tunes the expression of key FOXA2-activated lipid metabolism genes and is dysregulated in animal models of insulin resistance and diabetes. Diabetes. 2014;63:3141-8 pubmed publisher