This is a Validated Antibody Database (VAD) review about human A20, based on 28 published articles (read how Labome selects the articles), using A20 antibody in all methods. It is aimed to help Labome visitors find the most suited A20 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
A20 synonym: A20; AISBL; OTUD7C; TNFA1P2

Knockout validation
Santa Cruz Biotechnology
mouse monoclonal (A-12)
  • western blot knockout validation; mouse; loading ...; fig 8d, s8
Santa Cruz Biotechnology A20 antibody (Santa Cruz, sc-166692) was used in western blot knockout validation on mouse samples (fig 8d, s8). Nat Commun (2022) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-12)
  • western blot knockout validation; mouse; loading ...; fig s6a
Santa Cruz Biotechnology A20 antibody (Santa, sc-166692) was used in western blot knockout validation on mouse samples (fig s6a). Sci Signal (2021) ncbi
Abcam
mouse monoclonal (59A426)
  • immunocytochemistry; human; 1:200; loading ...; fig 1c
  • western blot knockout validation; mouse; 1:200; loading ...; fig 6b
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 2c
In order to research the effect of anti-inflammatory factor A20 on cerebral inflammatory injury, Abcam A20 antibody (Abcam, ab13597) was used in immunocytochemistry on human samples at 1:200 (fig 1c), in western blot knockout validation on mouse samples at 1:200 (fig 6b) and in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 2c). J Immunol (2017) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D13H3)
  • western blot knockout validation; mouse; fig s1a
  • western blot; human; loading ...; fig s6b
Cell Signaling Technology A20 antibody (Cell Signaling, 5630) was used in western blot knockout validation on mouse samples (fig s1a) and in western blot on human samples (fig s6b). J Clin Invest (2022) ncbi
Santa Cruz Biotechnology
mouse monoclonal (A-12)
  • western blot knockout validation; mouse; loading ...; fig 8d, s8
Santa Cruz Biotechnology A20 antibody (Santa Cruz, sc-166692) was used in western blot knockout validation on mouse samples (fig 8d, s8). Nat Commun (2022) ncbi
mouse monoclonal (A-12)
  • western blot knockout validation; mouse; loading ...; fig s6a
Santa Cruz Biotechnology A20 antibody (Santa, sc-166692) was used in western blot knockout validation on mouse samples (fig s6a). Sci Signal (2021) ncbi
mouse monoclonal (A-12)
  • flow cytometry; human; loading ...; fig 6b
Santa Cruz Biotechnology A20 antibody (Santa Cruz Biotechnology, A-12) was used in flow cytometry on human samples (fig 6b). Cell Mol Gastroenterol Hepatol (2021) ncbi
mouse monoclonal
  • flow cytometry; human; loading ...; fig 6b
Santa Cruz Biotechnology A20 antibody (Santa Cruz Biotechnology, A-12) was used in flow cytometry on human samples (fig 6b). Cell Mol Gastroenterol Hepatol (2021) ncbi
mouse monoclonal (4H16)
  • western blot; mouse; loading ...; fig 6c
Santa Cruz Biotechnology A20 antibody (anta Cruz Biotechnology, sc-69980) was used in western blot on mouse samples (fig 6c). Immunity (2019) ncbi
mouse monoclonal (A-12)
  • western blot; human; loading ...; fig 2a
Santa Cruz Biotechnology A20 antibody (Santa Cruz Biotechnology, sc-166692) was used in western blot on human samples (fig 2a). EMBO J (2017) ncbi
mouse monoclonal (A-12)
  • western blot; human; loading ...; fig 4a
In order to show that CARD14 activates p38 and JNK MAP kinase pathways, Santa Cruz Biotechnology A20 antibody (Santa Cruz, sc-166692) was used in western blot on human samples (fig 4a). EMBO Rep (2016) ncbi
mouse monoclonal (59A426)
  • western blot; human; loading ...; fig 4a
In order to show that CARD14 activates p38 and JNK MAP kinase pathways, Santa Cruz Biotechnology A20 antibody (Santa Cruz, sc-52910) was used in western blot on human samples (fig 4a). EMBO Rep (2016) ncbi
mouse monoclonal (59A426)
  • western blot; human; 1:1000; fig 3
Santa Cruz Biotechnology A20 antibody (Santa Cruz Biotechnology, 59A426) was used in western blot on human samples at 1:1000 (fig 3). J Cell Sci (2016) ncbi
mouse monoclonal (A-12)
  • immunohistochemistry - paraffin section; human; 1:200; fig 2
  • western blot; human; 1:1000; fig 1
Santa Cruz Biotechnology A20 antibody (Santa Cruz, sc-166692) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 2) and in western blot on human samples at 1:1000 (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (A-12)
  • immunoprecipitation; mouse; 1:50
  • western blot; mouse; 1:50
In order to report that autophaghy regulates NFkapaB activity through A20 sequestration, Santa Cruz Biotechnology A20 antibody (Santa, A-12) was used in immunoprecipitation on mouse samples at 1:50 and in western blot on mouse samples at 1:50. Nat Commun (2015) ncbi
mouse monoclonal (A-12)
  • western blot; mouse
Santa Cruz Biotechnology A20 antibody (Santa Cruz Biotech, sc-166692) was used in western blot on mouse samples . PLoS ONE (2014) ncbi
Abcam
mouse monoclonal (59A426)
  • western blot; mouse; 1:200; loading ...; fig 5e
Abcam A20 antibody (Abcam, ab13597) was used in western blot on mouse samples at 1:200 (fig 5e). Biology (Basel) (2022) ncbi
domestic rabbit monoclonal (EPR2663)
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 6e
  • immunocytochemistry; mouse; 1:200; loading ...; fig s8
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1b, 1e
  • immunocytochemistry; human; 1:200; loading ...; fig 1c
Abcam A20 antibody (Abcam, ab92324) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 6e), in immunocytochemistry on mouse samples at 1:200 (fig s8), in immunohistochemistry - paraffin section on human samples at 1:200 (fig 1b, 1e) and in immunocytochemistry on human samples at 1:200 (fig 1c). Nat Commun (2022) ncbi
domestic rabbit monoclonal (EPR2663)
  • western blot; human; 1:5000; fig 4b
Abcam A20 antibody (abcam, ab92324) was used in western blot on human samples at 1:5000 (fig 4b). Antioxidants (Basel) (2021) ncbi
mouse monoclonal (59A426)
  • immunocytochemistry; human; 1:200; loading ...; fig 1c
  • western blot knockout validation; mouse; 1:200; loading ...; fig 6b
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 2c
In order to research the effect of anti-inflammatory factor A20 on cerebral inflammatory injury, Abcam A20 antibody (Abcam, ab13597) was used in immunocytochemistry on human samples at 1:200 (fig 1c), in western blot knockout validation on mouse samples at 1:200 (fig 6b) and in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 2c). J Immunol (2017) ncbi
domestic rabbit monoclonal (EPR2663)
  • immunohistochemistry - paraffin section; human; 1:500; fig 1
  • western blot; human; 1:1000; fig s3
Abcam A20 antibody (Epitomics, 2644-1) was used in immunohistochemistry - paraffin section on human samples at 1:500 (fig 1) and in western blot on human samples at 1:1000 (fig s3). Oncotarget (2016) ncbi
mouse monoclonal (59A426)
  • immunohistochemistry; human; fig 5
Abcam A20 antibody (Abcam, ab13597) was used in immunohistochemistry on human samples (fig 5). Int J Biol Sci (2015) ncbi
domestic rabbit monoclonal (EPR2663)
  • western blot; human; 1:1000
Abcam A20 antibody (Abcam, ab92324) was used in western blot on human samples at 1:1000. Endocrinology (2015) ncbi
domestic rabbit monoclonal (EPR2663)
  • western blot; human; fig 3
In order to research osteoclast differentiation and need for NF-kappaB-direct activation of microRNAs with repressive effects on monocyte-specific genes, Abcam A20 antibody (Abcam, ab92324) was used in western blot on human samples (fig 3). Genome Biol (2015) ncbi
mouse monoclonal (59A426)
  • western blot; human; fig 6
Abcam A20 antibody (Abcam, ab13597) was used in western blot on human samples (fig 6). J Biol Chem (2014) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D13H3)
  • western blot knockout validation; mouse; fig s1a
  • western blot; human; loading ...; fig s6b
Cell Signaling Technology A20 antibody (Cell Signaling, 5630) was used in western blot knockout validation on mouse samples (fig s1a) and in western blot on human samples (fig s6b). J Clin Invest (2022) ncbi
domestic rabbit monoclonal (D13H3)
  • western blot; mouse; loading ...; fig 2e
Cell Signaling Technology A20 antibody (CST, 5630) was used in western blot on mouse samples (fig 2e). Nat Commun (2020) ncbi
domestic rabbit monoclonal (D13H3)
  • immunoprecipitation; human; loading ...; fig 2c
  • western blot; human; 1:1000; loading ...; fig 2c, 6a
Cell Signaling Technology A20 antibody (CST, D13H3) was used in immunoprecipitation on human samples (fig 2c) and in western blot on human samples at 1:1000 (fig 2c, 6a). Cell Death Dis (2020) ncbi
domestic rabbit monoclonal (D13H3)
  • western blot; human; 1:1000; loading ...; fig 5e
Cell Signaling Technology A20 antibody (Cell Signaling, D13H3) was used in western blot on human samples at 1:1000 (fig 5e). Front Immunol (2018) ncbi
domestic rabbit monoclonal (D13H3)
  • western blot; human; loading ...; fig 5f
Cell Signaling Technology A20 antibody (Cell Signaling Technology, 5630) was used in western blot on human samples (fig 5f). J Clin Invest (2018) ncbi
domestic rabbit monoclonal (D13H3)
  • western blot; mouse; loading ...; fig 1b
Cell Signaling Technology A20 antibody (Cell Signaling, 5630) was used in western blot on mouse samples (fig 1b). Cell Death Dis (2018) ncbi
domestic rabbit monoclonal (D13H3)
  • western blot; mouse; 1:1000; fig 2c
  • western blot; human; 1:1000; loading ...; fig 2c
Cell Signaling Technology A20 antibody (cell Signaling, 5630) was used in western blot on mouse samples at 1:1000 (fig 2c) and in western blot on human samples at 1:1000 (fig 2c). Nat Commun (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 6
In order to demonstrate that OTULIN is essential for preventing TNF-associated systemic inflammation in humans and mice, Cell Signaling Technology A20 antibody (Cell Signaling Tech, 4625) was used in western blot on mouse samples (fig 6). Cell (2016) ncbi
domestic rabbit monoclonal (D13H3)
  • western blot; human; loading ...; fig 1d
Cell Signaling Technology A20 antibody (Cell Signaling, 5630) was used in western blot on human samples (fig 1d). Oncotarget (2016) ncbi
domestic rabbit monoclonal (D13H3)
  • western blot; human; fig 1
Cell Signaling Technology A20 antibody (Cell signaling, 5630s) was used in western blot on human samples (fig 1). Int J Biol Sci (2015) ncbi
domestic rabbit monoclonal (D13H3)
  • flow cytometry; human; loading ...; fig 4a
Cell Signaling Technology A20 antibody (Cell Signaling, 5630S) was used in flow cytometry on human samples (fig 4a). Invest New Drugs (2015) ncbi
Articles Reviewed
  1. Papoutsopoulou S, Pollock L, Williams J, Abdul Mahdi M, Dobbash R, Duckworth C, et al. Interleukin-10 Deficiency Impacts on TNF-Induced NFκB Regulated Responses In Vivo. Biology (Basel). 2022;11: pubmed publisher
  2. Wang W, Bale S, Wei J, Yalavarthi B, Bhattacharyya D, Yan J, et al. Fibroblast A20 governs fibrosis susceptibility and its repression by DREAM promotes fibrosis in multiple organs. Nat Commun. 2022;13:6358 pubmed publisher
  3. Rusu I, Mennillo E, Bain J, Li Z, Sun X, Ly K, et al. Microbial signals, MyD88, and lymphotoxin drive TNF-independent intestinal epithelial tissue damage. J Clin Invest. 2022;132: pubmed publisher
  4. Son M, Wang A, Tu H, Metzig M, Patel P, Husain K, et al. NF-κB responds to absolute differences in cytokine concentrations. Sci Signal. 2021;14: pubmed
  5. Chang N, Yeh C, Lin Y, Kuo K, Fong I, Kounis N, et al. Garcinol Attenuates Lipoprotein(a)-Induced Oxidative Stress and Inflammatory Cytokine Production in Ventricular Cardiomyocyte through α7-Nicotinic Acetylcholine Receptor-Mediated Inhibition of the p38 MAPK and NF-κB Signaling Pathways. Antioxidants (Basel). 2021;10: pubmed publisher
  6. Li X, Zhang M, Huang X, Liang W, Li G, Lu X, et al. Ubiquitination of RIPK1 regulates its activation mediated by TNFR1 and TLRs signaling in distinct manners. Nat Commun. 2020;11:6364 pubmed publisher
  7. Sun R, Hedl M, Abraham C. TNFSF15 Promotes Antimicrobial Pathways in Human Macrophages and These Are Modulated by TNFSF15 Disease-Risk Variants. Cell Mol Gastroenterol Hepatol. 2021;11:249-272 pubmed publisher
  8. Feng Y, Zhang Y, Cai Y, Liu R, Lu M, Li T, et al. A20 targets PFKL and glycolysis to inhibit the progression of hepatocellular carcinoma. Cell Death Dis. 2020;11:89 pubmed publisher
  9. Arora H, Wilcox S, Johnson L, Munro L, Eyford B, Pfeifer C, et al. The ATP-Binding Cassette Gene ABCF1 Functions as an E2 Ubiquitin-Conjugating Enzyme Controlling Macrophage Polarization to Dampen Lethal Septic Shock. Immunity. 2019;50:418-431.e6 pubmed publisher
  10. Seeholzer T, Kurz S, Schlauderer F, Woods S, Gehring T, Widmann S, et al. BCL10-CARD11 Fusion Mimics an Active CARD11 Seed That Triggers Constitutive BCL10 Oligomerization and Lymphocyte Activation. Front Immunol. 2018;9:2695 pubmed publisher
  11. Bi E, Li R, Bover L, Li H, Su P, Ma X, et al. E-cadherin expression on multiple myeloma cells activates tumor-promoting properties in plasmacytoid DCs. J Clin Invest. 2018;128:4821-4831 pubmed publisher
  12. Siegmund D, Ehrenschwender M, Wajant H. TNFR2 unlocks a RIPK1 kinase activity-dependent mode of proinflammatory TNFR1 signaling. Cell Death Dis. 2018;9:921 pubmed publisher
  13. Sokhi U, Liber M, Frye L, Park S, Kang K, Pannellini T, et al. Dissection and function of autoimmunity-associated TNFAIP3 (A20) gene enhancers in humanized mouse models. Nat Commun. 2018;9:658 pubmed publisher
  14. Lafont E, Kantari Mimoun C, Dráber P, De Miguel D, Hartwig T, Reichert M, et al. The linear ubiquitin chain assembly complex regulates TRAIL-induced gene activation and cell death. EMBO J. 2017;36:1147-1166 pubmed publisher
  15. Meng Z, Zhao T, Zhou K, Zhong Q, Wang Y, Xiong X, et al. A20 Ameliorates Intracerebral Hemorrhage-Induced Inflammatory Injury by Regulating TRAF6 Polyubiquitination. J Immunol. 2017;198:820-831 pubmed publisher
  16. Damgaard R, Walker J, Marco Casanova P, Morgan N, Titheradge H, Elliott P, et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell. 2016;166:1215-1230.e20 pubmed publisher
  17. Song X, Yao Z, Yang J, Zhang Z, Deng Y, Li M, et al. HCV core protein binds to gC1qR to induce A20 expression and inhibit cytokine production through MAPKs and NF-κB signaling pathways. Oncotarget. 2016;7:33796-808 pubmed publisher
  18. Afonina I, Van Nuffel E, Baudelet G, Driege Y, Kreike M, Staal J, et al. The paracaspase MALT1 mediates CARD14-induced signaling in keratinocytes. EMBO Rep. 2016;17:914-27 pubmed publisher
  19. Douanne T, Gavard J, Bidère N. The paracaspase MALT1 cleaves the LUBAC subunit HOIL1 during antigen receptor signaling. J Cell Sci. 2016;129:1775-80 pubmed publisher
  20. Wang X, Ma C, Zong Z, Xiao Y, Li N, Guo C, et al. A20 inhibits the motility of HCC cells induced by TNF-α. Oncotarget. 2016;7:14742-54 pubmed publisher
  21. Ai L, Xu Q, Wu C, Wang X, Chen Z, Su D, et al. A20 Attenuates FFAs-induced Lipid Accumulation in Nonalcoholic Steatohepatitis. Int J Biol Sci. 2015;11:1436-46 pubmed publisher
  22. Wang Y, Wan M, Zhou Q, Wang H, Wang Z, Zhong X, et al. The Prognostic Role of SOCS3 and A20 in Human Cholangiocarcinoma. PLoS ONE. 2015;10:e0141165 pubmed publisher
  23. Zhong X, Liao Y, Chen L, Liu G, Feng Y, Zeng T, et al. The MicroRNAs in the Pathogenesis of Metabolic Memory. Endocrinology. 2015;156:3157-68 pubmed publisher
  24. Kanayama M, Inoue M, Danzaki K, Hammer G, He Y, Shinohara M. Autophagy enhances NFκB activity in specific tissue macrophages by sequestering A20 to boost antifungal immunity. Nat Commun. 2015;6:5779 pubmed publisher
  25. de la Rica L, García Gómez A, Comet N, Rodríguez Ubreva J, Ciudad L, Vento Tormo R, et al. NF-κB-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation. Genome Biol. 2015;16:2 pubmed publisher
  26. Guzmán E, Maers K, Roberts J, Kemami Wangun H, Harmody D, Wright A. The marine natural product microsclerodermin A is a novel inhibitor of the nuclear factor kappa B and induces apoptosis in pancreatic cancer cells. Invest New Drugs. 2015;33:86-94 pubmed publisher
  27. Kasama Y, Mizukami T, Kusunoki H, Peveling Oberhag J, Nishito Y, Ozawa M, et al. B-cell-intrinsic hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-?B signalling. PLoS ONE. 2014;9:e91373 pubmed publisher
  28. Altonsy M, Sasse S, Phang T, Gerber A. Context-dependent cooperation between nuclear factor ?B (NF-?B) and the glucocorticoid receptor at a TNFAIP3 intronic enhancer: a mechanism to maintain negative feedback control of inflammation. J Biol Chem. 2014;289:8231-9 pubmed publisher