This is a Validated Antibody Database (VAD) review about guinea pig Pln, based on 16 published articles (read how Labome selects the articles), using Pln antibody in all methods. It is aimed to help Labome visitors find the most suited Pln antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Invitrogen
mouse monoclonal (2D12)
  • western blot; mouse; loading ...; fig 4i
Invitrogen Pln antibody (Thermo Fisher Scientific, 2D12) was used in western blot on mouse samples (fig 4i). Sci Adv (2022) ncbi
mouse monoclonal (2D12)
  • western blot; domestic rabbit; 1:2000; loading ...; fig 5
In order to report the effects of heart failure on the electrical activity of sinoatrial nodes with and without pharmacological interventions, Invitrogen Pln antibody (Thermo Fisher, MA3-922) was used in western blot on domestic rabbit samples at 1:2000 (fig 5). Exp Ther Med (2017) ncbi
mouse monoclonal (2D12)
  • western blot; mouse; loading ...; fig 1b
In order to report the physiological role of sarcolipin upregulation in muscle myopathy, Invitrogen Pln antibody (Pierce Antibodies, 2D12) was used in western blot on mouse samples (fig 1b). PLoS ONE (2017) ncbi
mouse monoclonal (2D12)
  • western blot; rat; fig 8b
In order to analyze cardiomyocyte function in dependence of TRPC4 splice variant expression., Invitrogen Pln antibody (Millipore, MA3-922) was used in western blot on rat samples (fig 8b). PLoS ONE (2016) ncbi
mouse monoclonal (2D12)
  • western blot; mouse; 1:1000; loading ...; fig 2c
In order to evaluate the impacts of high fat diet on the insulin-adrenergic receptor signal network in hearts, Invitrogen Pln antibody (Affinity Bioreagent, MA3-922) was used in western blot on mouse samples at 1:1000 (fig 2c). J Physiol (2017) ncbi
mouse monoclonal (2D12)
  • western blot; mouse; 1:2000; fig 1
In order to study slow-twitch type 1 muscle fibers and diaphragm assessment in mice overexpressing phospholamban, Invitrogen Pln antibody (Pierce Antibodies, 2D12) was used in western blot on mouse samples at 1:2000 (fig 1). Brain Behav (2016) ncbi
mouse monoclonal (2D12)
  • western blot; rat; 1:5000; fig s1
In order to investigate left ventricular cardiomyocytes from post-infaction rats with and without heart failure after beta-adrenoceptor stimulation to revela Ca2+ waves and sarcoplasmic reticulum Ca2+ depletion, Invitrogen Pln antibody (Thermo Fisher Scientific, MA3-922) was used in western blot on rat samples at 1:5000 (fig s1). PLoS ONE (2016) ncbi
mouse monoclonal (2D12)
  • western blot; mouse; 1:5000; loading ...; fig s9a
In order to elucidate how DWORF activates the SERCA pump, Invitrogen Pln antibody (Pierce, 2D12) was used in western blot on mouse samples at 1:5000 (fig s9a). Science (2016) ncbi
mouse monoclonal (2D12)
  • western blot; mouse
  • western blot; human
In order to study SERCA dysfunction in mice overexpressing phospholamban, Invitrogen Pln antibody (Pierce Antibodies, 2D12) was used in western blot on mouse samples and in western blot on human samples . Dis Model Mech (2015) ncbi
mouse monoclonal (2D12)
  • western blot; rat; fig 5
In order to determine the relationship between ACE and ACE2 activity, left ventricular function, and gender in spontaneously hypertensive rats, Invitrogen Pln antibody (Affinity BioReagents, MA3-922) was used in western blot on rat samples (fig 5). PLoS ONE (2015) ncbi
mouse monoclonal (2D12)
  • western blot; mouse; 1:20,000
In order to study the relationship between the E2F pathway, beta2-adrenergic/PKA/PDE4D, and ERK/c-Src axis during the pathological hypertrophic growth response, Invitrogen Pln antibody (Affinity Bioreagents, MA3-922) was used in western blot on mouse samples at 1:20,000. J Mol Cell Cardiol (2015) ncbi
mouse monoclonal (2D12)
  • western blot; domestic rabbit
In order to study the effect of progesterone on SERCA2a regulation in isolated cardiomyocytes, Invitrogen Pln antibody (Thermo Scientific, MA3-922) was used in western blot on domestic rabbit samples . Am J Physiol Cell Physiol (2014) ncbi
mouse monoclonal (2D12)
  • western blot; rat; 1:5000; fig 5
In order to learn that imbalance between myocardial phospholamban dephosphorylation and phosphorylation is not caused by obesity, Invitrogen Pln antibody (Thermo Scientific, MA3-922) was used in western blot on rat samples at 1:5000 (fig 5). Arq Bras Cardiol (2014) ncbi
mouse monoclonal (2D12)
  • western blot; mouse
In order to describe a protocol to determine the affinity of Ca(2+) to the Ca(2+) pump in crude homogenates of mouse heart., Invitrogen Pln antibody (Affinity BioReagents, MA3-922) was used in western blot on mouse samples . Cold Spring Harb Protoc (2014) ncbi
mouse monoclonal (2D12)
  • immunohistochemistry - frozen section; human; 1:250; fig 4
  • immunoprecipitation; human; fig 6
  • western blot; human; 1:2000; fig 3
In order to determine if sarcolipin and phospholamban regulate SERCA1a, SERCA2a, or both in skeletal muscle, Invitrogen Pln antibody (Pierce, 2D12) was used in immunohistochemistry - frozen section on human samples at 1:250 (fig 4), in immunoprecipitation on human samples (fig 6) and in western blot on human samples at 1:2000 (fig 3). PLoS ONE (2013) ncbi
mouse monoclonal (2D12)
  • western blot; human
In order to investigate the abnormalities of calcium handling proteins in skeletal and heart muscle during heart failure, Invitrogen Pln antibody (Pierce, 2D12) was used in western blot on human samples . J Card Fail (2012) ncbi
Articles Reviewed
  1. Contreras W, Wieseh xf6 fer C, Schreier D, Leinung N, Peche P, Wennemuth G, et al. C11orf94/Frey is a key regulator for male fertility by controlling Izumo1 complex assembly. Sci Adv. 2022;8:eabo6049 pubmed publisher
  2. Chang S, Chuang H, Chen Y, Kao Y, Lin Y, Yeh Y, et al. Heart failure modulates electropharmacological characteristics of sinoatrial nodes. Exp Ther Med. 2017;13:771-779 pubmed publisher
  3. Fajardo V, Gamu D, Mitchell A, Bloemberg D, Bombardier E, Chambers P, et al. Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice. PLoS ONE. 2017;12:e0173708 pubmed publisher
  4. Kirschmer N, Bandleon S, von Ehrlich Treuenstätt V, Hartmann S, Schaaf A, Lamprecht A, et al. TRPC4? and TRPC4? Similarly Affect Neonatal Cardiomyocyte Survival during Chronic GPCR Stimulation. PLoS ONE. 2016;11:e0168446 pubmed publisher
  5. Fu Q, Hu Y, Wang Q, Liu Y, Li N, Xu B, et al. High-fat diet induces protein kinase A and G-protein receptor kinase phosphorylation of β2 -adrenergic receptor and impairs cardiac adrenergic reserve in animal hearts. J Physiol. 2017;595:1973-1986 pubmed publisher
  6. Fajardo V, Smith I, Bombardier E, Chambers P, Quadrilatero J, Tupling A. Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers. Brain Behav. 2016;6:e00470 pubmed publisher
  7. Sadredini M, Danielsen T, Aronsen J, Manotheepan R, Hougen K, Sjaastad I, et al. Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure. PLoS ONE. 2016;11:e0153887 pubmed publisher
  8. Nelson B, Makarewich C, Anderson D, Winders B, Troupes C, Wu F, et al. A peptide encoded by a transcript annotated as long noncoding RNA enhances SERCA activity in muscle. Science. 2016;351:271-5 pubmed publisher
  9. Fajardo V, Bombardier E, McMillan E, TRAN K, Wadsworth B, Gamu D, et al. Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype. Dis Model Mech. 2015;8:999-1009 pubmed publisher
  10. Dalpiaz P, Lamas A, Caliman I, Ribeiro R, Abreu G, Moyses M, et al. Sex Hormones Promote Opposite Effects on ACE and ACE2 Activity, Hypertrophy and Cardiac Contractility in Spontaneously Hypertensive Rats. PLoS ONE. 2015;10:e0127515 pubmed publisher
  11. Major J, Salih M, Tuana B. Interplay between the E2F pathway and β-adrenergic signaling in the pathological hypertrophic response of myocardium. J Mol Cell Cardiol. 2015;84:179-90 pubmed publisher
  12. Moshal K, Zhang Z, Roder K, Kim T, Cooper L, Patedakis Litvinov B, et al. Progesterone modulates SERCA2a expression and function in rabbit cardiomyocytes. Am J Physiol Cell Physiol. 2014;307:C1050-7 pubmed publisher
  13. Freire P, Alves C, Deus A, Leopoldo A, Leopoldo A, Silva D, et al. Obesity does not lead to imbalance between myocardial phospholamban phosphorylation and dephosphorylation. Arq Bras Cardiol. 2014;103:41-50 pubmed
  14. Holemans T, Vandecaetsbeek I, Wuytack F, Vangheluwe P. Measuring Ca2+-dependent Ca2+-uptake activity in the mouse heart. Cold Spring Harb Protoc. 2014;2014:876-86 pubmed publisher
  15. Fajardo V, Bombardier E, Vigna C, Devji T, Bloemberg D, Gamu D, et al. Co-expression of SERCA isoforms, phospholamban and sarcolipin in human skeletal muscle fibers. PLoS ONE. 2013;8:e84304 pubmed publisher
  16. Middlekauff H, Vigna C, Verity M, Fonarow G, Horwich T, Hamilton M, et al. Abnormalities of calcium handling proteins in skeletal muscle mirror those of the heart in humans with heart failure: a shared mechanism?. J Card Fail. 2012;18:724-33 pubmed publisher