This is a Validated Antibody Database (VAD) review about fruit fly alpha-Spec, based on 6 published articles (read how Labome selects the articles), using alpha-Spec antibody in all methods. It is aimed to help Labome visitors find the most suited alpha-Spec antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
alpha-Spec synonym: 3A9; CG1977; Dmel\CG1977; FBtr0072789; FCP-B; Spec; Spectrin; alfa-Spec; alpha; alpha spectrin; alpha-SPEC; alpha-Sp; alpha-Spc; alpha-Spe; alpha-Spect; alpha-Spectrin; alpha-spec; alpha-spectrin; alphaSp; alphaSpec; alphaSpectrin; anon-EST:fe2E2; dre3; klo; l(3)04276; l(3)62Bd; l(3)alpha-Spec; l(3)dre3; spectrin; alpha spectrin; CG1977-PA; CG1977-PB; CG1977-PC; CG1977-PD; aSpc 3A9; alpha-SPECTRIN; alpha-Spec-PA; alpha-Spec-PB; alpha-Spec-PC; alpha-Spec-PD; alpha-spectrin II; alphaSpectrin; anon-fast-evolving-2E2; klotzchen; spectrin

Developmental Studies Hybridoma Bank
mouse monoclonal (3A9 (323 or M10-2))
  • immunohistochemistry; fruit fly; 1:25; loading ...; tbl 3
Developmental Studies Hybridoma Bank alpha-Spec antibody (DSHB, 3A9) was used in immunohistochemistry on fruit fly samples at 1:25 (tbl 3). elife (2015) ncbi
mouse monoclonal (3A9 (323 or M10-2))
  • immunohistochemistry; fruit fly; 1:25
In order to describe a new method to generate designer genes: Gene targeting during Oogenesis with Lethality Inhibitor and CRISPR/Cas (Golic+), Developmental Studies Hybridoma Bank alpha-Spec antibody (DSHB, 3A9) was used in immunohistochemistry on fruit fly samples at 1:25. Genetics (2015) ncbi
mouse monoclonal (3A9 (323 or M10-2))
  • immunohistochemistry; fruit fly; 1:5
In order to study Nasrat and Polehole during development, Developmental Studies Hybridoma Bank alpha-Spec antibody (Developmental Studies Hybridoma Bank, 3A9) was used in immunohistochemistry on fruit fly samples at 1:5. Fly (Austin) (2014) ncbi
mouse monoclonal (3A9 (323 or M10-2))
  • immunocytochemistry; fruit fly; 1:50
In order to determine the contribution of Tut, Bam, and Bgcn in the proliferation and differentiation of the Drosophila germline stem cell lineage, Developmental Studies Hybridoma Bank alpha-Spec antibody (DSHB, 3A9) was used in immunocytochemistry on fruit fly samples at 1:50. PLoS Genet (2014) ncbi
mouse monoclonal (3A9 (323 or M10-2))
  • immunohistochemistry; fruit fly; 1:20; fig 1
In order to study a stress assembly that supports cell viability by preserving ERES components during amino-acid starvation, Developmental Studies Hybridoma Bank alpha-Spec antibody (DSHB, 3A9) was used in immunohistochemistry on fruit fly samples at 1:20 (fig 1). elife (2014) ncbi
mouse monoclonal (3A9 (323 or M10-2))
  • immunohistochemistry - free floating section; fruit fly; 1:20
  • western blot; fruit fly; 1:1000-10,000
Developmental Studies Hybridoma Bank alpha-Spec antibody (The Developmental Studies Hybridoma Bank, 3A9) was used in immunohistochemistry - free floating section on fruit fly samples at 1:20 and in western blot on fruit fly samples at 1:1000-10,000. J Biol Chem (2015) ncbi
Articles Reviewed
  1. Flores Benitez D, Knust E. Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila. elife. 2015;4: pubmed publisher
  2. Chen H, Huang Y, Pfeiffer B, Yao X, Lee T. An enhanced gene targeting toolkit for Drosophila: Golic+. Genetics. 2015;199:683-94 pubmed publisher
  3. Furriols M, Casanova J. Germline and somatic vitelline proteins colocalize in aggregates in the follicular epithelium of Drosophila ovaries. Fly (Austin). 2014;8:113-9 pubmed publisher
  4. Chen D, Wu C, Zhao S, Geng Q, Gao Y, Li X, et al. Three RNA binding proteins form a complex to promote differentiation of germline stem cell lineage in Drosophila. PLoS Genet. 2014;10:e1004797 pubmed publisher
  5. Zacharogianni M, Aguilera Gomez A, Veenendaal T, Smout J, Rabouille C. A stress assembly that confers cell viability by preserving ERES components during amino-acid starvation. elife. 2014;3: pubmed publisher
  6. Khanna M, Mattie F, Browder K, Radyk M, Crilly S, Bakerink K, et al. Spectrin tetramer formation is not required for viable development in Drosophila. J Biol Chem. 2015;290:706-15 pubmed publisher