This is a Validated Antibody Database (VAD) review about fruit fly Atxn7, based on 4 published articles (read how Labome selects the articles), using Atxn7 antibody in all methods. It is aimed to help Labome visitors find the most suited Atxn7 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Atxn7 synonym: CG9866; Dmel\CG9866; ataxin 7; Ataxin-7; Atxn7-PA; Atxn7-PB; Atxn7-PC; Atxn7-PD; CG9866-PA; CG9866-PB; CG9866-PC; CG9866-PD

Invitrogen
rabbit polyclonal
  • immunocytochemistry; human; 1:100; loading ...; fig 1c
In order to suggest that the selective targeting of a repeat tract in a mutant ATXN7 transcript by RNA interference is a feasible approach to treat spinocerebellar ataxia type 7, Invitrogen Atxn7 antibody (ThermoFisher Scientific, PA1-749) was used in immunocytochemistry on human samples at 1:100 (fig 1c). Genes (Basel) (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:2000; fig 1
  • western blot; human; 1:2000; fig 1
In order to study recapitulation of SCA7 pathology and promotion of accumulation of the FUS/TLS and MBNL1 RNA-binding proteins by lentiviral vector-mediated overexpression of mutant ataxin-7, Invitrogen Atxn7 antibody (Thermo Scientific, PA1-749) was used in immunohistochemistry - frozen section on human samples at 1:2000 (fig 1) and in western blot on human samples at 1:2000 (fig 1). Mol Neurodegener (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:300; fig 3
  • western blot; human; fig 1
In order to determine if caspase cleavage contributes to spinocerebellar ataxia type 7 disease pathogenesis using transgenic mice, Invitrogen Atxn7 antibody (Affinity BioReagents, PA1-749) was used in immunohistochemistry on human samples at 1:300 (fig 3) and in western blot on human samples (fig 1). Hum Mol Genet (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; human; 1:1000
  • western blot; human
In order to test if reducing ataxin-7 in Purkinje cells is beneficial in an animal model of SCA7, Invitrogen Atxn7 antibody (Thermo, PA1-749) was used in immunohistochemistry - free floating section on human samples at 1:1000 and in western blot on human samples . Mol Ther (2014) ncbi
Articles Reviewed
  1. Fiszer A, Wroblewska J, Nowak B, Krzyzosiak W. Mutant CAG Repeats Effectively Targeted by RNA Interference in SCA7 Cells. Genes (Basel). 2016;7: pubmed publisher
  2. Alves S, Marais T, Biferi M, Furling D, Marinello M, El Hachimi K, et al. Lentiviral vector-mediated overexpression of mutant ataxin-7 recapitulates SCA7 pathology and promotes accumulation of the FUS/TLS and MBNL1 RNA-binding proteins. Mol Neurodegener. 2016;11:58 pubmed publisher
  3. Guyenet S, Mookerjee S, Lin A, Custer S, Chen S, Sopher B, et al. Proteolytic cleavage of ataxin-7 promotes SCA7 retinal degeneration and neurological dysfunction. Hum Mol Genet. 2015;24:3908-17 pubmed publisher
  4. Ramachandran P, Boudreau R, Schaefer K, La Spada A, Davidson B. Nonallele specific silencing of ataxin-7 improves disease phenotypes in a mouse model of SCA7. Mol Ther. 2014;22:1635-42 pubmed publisher