This is a Validated Antibody Database (VAD) review about fruit fly.. Hsp70Aa, based on 32 published articles (read how Labome selects the articles), using Hsp70Aa antibody in all methods. It is aimed to help Labome visitors find the most suited Hsp70Aa antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Hsp70Aa synonym: 87A; CG31366; Dm-hsp70; DmHSP70AA; DmelCG31366; HSP 70; HSP70; Hsp 70; Hsp-70; Hsp-70Aa; Hsp70; Hsp70A; Hsp70a; dHSP70; dHsp70; dhsp70; dhsp70Aa; hs70; hsp 70; hsp-70; hsp70; hsp70 87A; hsp70 Aa; hsp70(87A); hsp70A; hsp70Aa; hsp70a

Knockout validation
Abcam
mouse monoclonal (C92F3A-5)
  • western blot knockout validation; human; 1:5000; loading ...; fig 1b
Abcam Hsp70Aa antibody (Abcam, ab47454) was used in western blot knockout validation on human samples at 1:5000 (fig 1b). elife (2020) ncbi
Abcam
mouse monoclonal (C92F3A-5)
  • western blot knockout validation; human; 1:5000; loading ...; fig 1b
Abcam Hsp70Aa antibody (Abcam, ab47454) was used in western blot knockout validation on human samples at 1:5000 (fig 1b). elife (2020) ncbi
mouse monoclonal (C92F3A-5)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 1f
  • immunocytochemistry; human; 1:100; loading ...; fig 2i
Abcam Hsp70Aa antibody (Abcam, ab47455) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1f) and in immunocytochemistry on human samples at 1:100 (fig 2i). Nat Cell Biol (2020) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; mouse; 1:1000; loading ...; fig 3b
Abcam Hsp70Aa antibody (Abcam, ab47455) was used in western blot on mouse samples at 1:1000 (fig 3b). Stress (2019) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; human; loading ...; fig s3b
In order to study the suppressive effect of DNAJB6 and Hsp70 on alpha-synuclein aggregation, Abcam Hsp70Aa antibody (Abcam, ab47455) was used in western blot on human samples (fig s3b). Sci Rep (2017) ncbi
mouse monoclonal (2A4)
  • immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 1c
In order to examine the structural and ultra-structural changes induced by nitrate in rat liver and assess the protective role of s-allylcysteine, Abcam Hsp70Aa antibody (Abcam, ab5442) was used in immunohistochemistry - paraffin section on rat samples at 1:200 (fig 1c). Ultrastruct Pathol (2017) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; human; 1:1000; fig 1
In order to research differentiation of functional glutamatergic neurons from placenta-derived multipotent cells by knocking down of heat-shock protein 27, Abcam Hsp70Aa antibody (Abcam, ab47455) was used in western blot on human samples at 1:1000 (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; rat; 1:1000; loading ...; fig 3b
Abcam Hsp70Aa antibody (Abcam, ab47455) was used in western blot on rat samples at 1:1000 (fig 3b). In Vitro Cell Dev Biol Anim (2016) ncbi
mouse monoclonal (2A4)
  • immunohistochemistry - paraffin section; human; loading ...; tbl 2
Abcam Hsp70Aa antibody (Abcam, 2A4) was used in immunohistochemistry - paraffin section on human samples (tbl 2). J Transl Med (2016) ncbi
mouse monoclonal (C92F3A-5)
  • immunoprecipitation; human; loading ...; fig 2a
  • immunocytochemistry; human; loading ...; fig 3d
  • western blot; human; loading ...; fig 3c
Abcam Hsp70Aa antibody (Abcam, ab47455) was used in immunoprecipitation on human samples (fig 2a), in immunocytochemistry on human samples (fig 3d) and in western blot on human samples (fig 3c). FASEB J (2016) ncbi
mouse monoclonal (2A4)
  • western blot; rat; 1:1000; fig 1
Abcam Hsp70Aa antibody (Abcam, ab5442) was used in western blot on rat samples at 1:1000 (fig 1). Int J Mol Med (2016) ncbi
mouse monoclonal (2A4)
  • ELISA; Austrofundulus limnaeus; 1:1000; loading ...; fig 6a
In order to analyze chromatin in vertebrate embryo diapause, Abcam Hsp70Aa antibody (Abcam, ab5442) was used in ELISA on Austrofundulus limnaeus samples at 1:1000 (fig 6a). J Exp Biol (2016) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; African green monkey
In order to perform a proteomic analysis of host proteins in Rift Valley fever virus virions and to study the particular significance of Hsp90, Abcam Hsp70Aa antibody (Abcam, ab47455) was used in western blot on African green monkey samples . PLoS ONE (2014) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; human; fig s3b
Abcam Hsp70Aa antibody (Abcam, ab47455) was used in western blot on human samples (fig s3b). PLoS ONE (2012) ncbi
Invitrogen
mouse monoclonal (3A3)
  • western blot; human; 1:200; loading ...; fig 5
In order to elucidate the antiproliferative and pro-apoptotic effects of thiazine[6,5-b]indole, Invitrogen Hsp70Aa antibody (Affinity BioReagents, MA3-006) was used in western blot on human samples at 1:200 (fig 5). Biomed Pharmacother (2017) ncbi
mouse monoclonal (3A3)
In order to measure biochemical and organismal responses to acute heat stress on two neritic copepods, Invitrogen Hsp70Aa antibody (Thermo Scientific, MA3-006) was used . Comp Biochem Physiol A Mol Integr Physiol (2017) ncbi
mouse monoclonal (2A4)
  • western blot; human; 1:5000; loading ...; fig 1a
In order to demonstrate the bipartite role of Hsp90 in chaperoning CRAF kinase, Invitrogen Hsp70Aa antibody (Thermo Pierce, MA3-008) was used in western blot on human samples at 1:5000 (fig 1a). J Biol Chem (2016) ncbi
mouse monoclonal (3A3)
  • western blot; Artemia franciscana; 1:5000; fig 2a
  • western blot; human; 1:5000
In order to test if exposure to non-lethal heat shock increases the tolerance of Artemia franciscana to subsequent metal exposure, Invitrogen Hsp70Aa antibody (Affinity BioReagents, 3A3) was used in western blot on Artemia franciscana samples at 1:5000 (fig 2a) and in western blot on human samples at 1:5000. Environ Res (2016) ncbi
mouse monoclonal (3A3)
  • western blot; mouse; 1:1000; loading ...; fig 3c
In order to investigate the function of extended synaptotagmins in knock out mice, Invitrogen Hsp70Aa antibody (Thermo Scientific, MA3-0066) was used in western blot on mouse samples at 1:1000 (fig 3c). PLoS ONE (2016) ncbi
mouse monoclonal (3A3)
In order to assess the effect of salinity on catfish's susceptibility to bacterial infection, Invitrogen Hsp70Aa antibody (ThermoScientific, 3A3) was used . Fish Shellfish Immunol (2016) ncbi
mouse monoclonal (3A3)
  • western blot; Pacific oyster; 1:2500; fig 1
In order to study salinity and thermal stress in wild and farmed Pacific oysters Crassostrea gigas, Invitrogen Hsp70Aa antibody (Affinity BioReagents, MA3-006) was used in western blot on Pacific oyster samples at 1:2500 (fig 1). Comp Biochem Physiol A Mol Integr Physiol (2016) ncbi
mouse monoclonal (5A5)
  • western blot; scallops; fig 7
In order to determine the affect of cellular bioenergetics in marine molluscs and functional reorganization of mitochondria by intermittent hypoxia, Invitrogen Hsp70Aa antibody (Affinity Bioreagents, MA3-007) was used in western blot on scallops samples (fig 7). J Exp Biol (2016) ncbi
mouse monoclonal (5A5)
  • western blot; human; fig 1
In order to calculate how ionizing radiation can promote cell survival in exosomes derived from squamous head and neck cancer, Invitrogen Hsp70Aa antibody (Affinity Bioreagents, MA3-007) was used in western blot on human samples (fig 1). PLoS ONE (2016) ncbi
mouse monoclonal (3A3)
  • western blot; mouse; 1:5000; fig 1
In order to determine the affects of dendritic cell migration in vitro by loss of gadkin, Invitrogen Hsp70Aa antibody (Affinity Bioreagents, MA3006) was used in western blot on mouse samples at 1:5000 (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (3A3)
  • western blot; common limpet; 1:1000
In order to examine the effect of sun exposure on of thermal refugia, Invitrogen Hsp70Aa antibody (Thermo Scientific, MA3-006) was used in western blot on common limpet samples at 1:1000. Glob Chang Biol (2016) ncbi
mouse monoclonal (3A3)
  • western blot; Asian green mussel; 1:5000; fig 2
In order to test if non-lethal heat shock of Perna viridis promotes the production of Hsp70 and enhances its resistance to stresses, Invitrogen Hsp70Aa antibody (Thermo Scientific, MA3-006) was used in western blot on Asian green mussel samples at 1:5000 (fig 2). PLoS ONE (2015) ncbi
mouse monoclonal (3A3)
  • immunohistochemistry; holothurians; 1:200
  • western blot; holothurians; 1:1000
In order to study HSP70 in Apostichopus japonicus, Invitrogen Hsp70Aa antibody (Thermo, MA3-006) was used in immunohistochemistry on holothurians samples at 1:200 and in western blot on holothurians samples at 1:1000. Fish Shellfish Immunol (2015) ncbi
mouse monoclonal (5A5)
  • western blot; mouse
In order to elucidate the factors that mediate radiation-induced endothelial dysfunction, Invitrogen Hsp70Aa antibody (scbt, MA3-007) was used in western blot on mouse samples . J Proteome Res (2015) ncbi
mouse monoclonal (3A3)
  • western blot; mouse; fig 2
In order to study the temporal regulation of MyoD in skeletal muscle differentiation, Invitrogen Hsp70Aa antibody (ABR Affinity Bio Reagents, MA3-006) was used in western blot on mouse samples (fig 2). Dev Dyn (2015) ncbi
mouse monoclonal (5A5)
  • ELISA; fruit fly ; 1:1000; fig 3
In order to test the thermal co-adaptation hypothesis using Drosophila subobscura, Invitrogen Hsp70Aa antibody (Thermo Scientific, 5A5) was used in ELISA on fruit fly samples at 1:1000 (fig 3). J Evol Biol (2012) ncbi
mouse monoclonal (5A5)
  • ELISA; Lycaena tityrus; 1:700; loading ...; fig 2c
In order to evaluate if the use of constant temperatures is justified when studying Lycaena tityrus, Invitrogen Hsp70Aa antibody (Affinity BioReagents, 5A5) was used in ELISA on Lycaena tityrus samples at 1:700 (fig 2c). Oecologia (2011) ncbi
mouse monoclonal (3A3)
  • western blot; human; 1:3000
  • western blot; Marenzelleria viridis; 1:3000; fig 7a
In order to study the effect of acclimation salinity on the stress protein response in two sibling species of Marenzelleria, Invitrogen Hsp70Aa antibody (Affinity BioReagents, MA3-006) was used in western blot on human samples at 1:3000 and in western blot on Marenzelleria viridis samples at 1:3000 (fig 7a). Comp Biochem Physiol B Biochem Mol Biol (2006) ncbi
mouse monoclonal (3A3)
  • western blot; rat; loading ...; fig 2a
In order to find factors that interact with N-myristoylated Arf6, Invitrogen Hsp70Aa antibody (Affinity Bioreagents, 3A3) was used in western blot on rat samples (fig 2a). Methods Enzymol (2005) ncbi
Articles Reviewed
  1. Kuncha S, Venkadasamy V, Amudhan G, Dahate P, Kola S, Pottabathini S, et al. Genomic innovation of ATD alleviates mistranslation associated with multicellularity in Animalia. elife. 2020;9: pubmed publisher
  2. Gaglia G, Rashid R, Yapp C, Joshi G, Li C, Lindquist S, et al. HSF1 phase transition mediates stress adaptation and cell fate decisions. Nat Cell Biol. 2020;22:151-158 pubmed publisher
  3. Shi H, Yao R, Lian S, Liu P, Liu Y, Yang Y, et al. Regulating glycolysis, the TLR4 signal pathway and expression of RBM3 in mouse liver in response to acute cold exposure. Stress. 2019;22:366-376 pubmed publisher
  4. Aprile F, Källstig E, Limorenko G, Vendruscolo M, Ron D, Hansen C. The molecular chaperones DNAJB6 and Hsp70 cooperate to suppress α-synuclein aggregation. Sci Rep. 2017;7:9039 pubmed publisher
  5. Kattaia A, Abd El Baset S, Mohamed E, Abdul Maksou R, Elfakharany Y. Molecular mechanisms underlying histological and biochemical changes induced by nitrate in rat liver and the efficacy of S-Allylcysteine. Ultrastruct Pathol. 2017;41:10-22 pubmed publisher
  6. Solárová Z, Kello M, Varinska L, Budovská M, Solar P. Inhibition of heat shock protein (Hsp) 90 potentiates the antiproliferative and pro-apoptotic effects of 2-(4'fluoro-phenylamino)-4H-1,3-thiazine[6,5-b]indole in A2780cis cells. Biomed Pharmacother. 2017;85:463-471 pubmed publisher
  7. Rahlff J, Peters J, Moyano M, Pless O, Claussen C, Peck M. Short-term molecular and physiological responses to heat stress in neritic copepods Acartia tonsa and Eurytemora affinis. Comp Biochem Physiol A Mol Integr Physiol. 2017;203:348-358 pubmed publisher
  8. Mitra S, Ghosh B, Gayen N, Roy J, Mandal A. Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation. J Biol Chem. 2016;291:24579-24593 pubmed
  9. Pestana J, Novais S, Norouzitallab P, Vandegehuchte M, Bossier P, De Schamphelaere K. Non-lethal heat shock increases tolerance to metal exposure in brine shrimp. Environ Res. 2016;151:663-670 pubmed publisher
  10. Cheng Y, Huang C, Lee Y, Tien L, Ku W, Chien R, et al. Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells. Sci Rep. 2016;6:30314 pubmed publisher
  11. Sclip A, Bacaj T, Giam L, Sudhof T. Extended Synaptotagmin (ESyt) Triple Knock-Out Mice Are Viable and Fertile without Obvious Endoplasmic Reticulum Dysfunction. PLoS ONE. 2016;11:e0158295 pubmed publisher
  12. Schmitz M, Douxfils J, Mandiki S, Morana C, Baekelandt S, Kestemont P. Chronic hyperosmotic stress interferes with immune homeostasis in striped catfish (Pangasianodon hypophthalmus, S.) and leads to excessive inflammatory response during bacterial infection. Fish Shellfish Immunol. 2016;55:550-8 pubmed publisher
  13. Yang C, Sierp M, Abbott C, Li Y, Qin J. Responses to thermal and salinity stress in wild and farmed Pacific oysters Crassostrea gigas. Comp Biochem Physiol A Mol Integr Physiol. 2016;201:22-29 pubmed publisher
  14. Ivanina A, Nesmelova I, Leamy L, Sokolov E, Sokolova I. Intermittent hypoxia leads to functional reorganization of mitochondria and affects cellular bioenergetics in marine molluscs. J Exp Biol. 2016;219:1659-74 pubmed publisher
  15. Mutschelknaus L, Peters C, Winkler K, Yentrapalli R, Heider T, Atkinson M, et al. Exosomes Derived from Squamous Head and Neck Cancer Promote Cell Survival after Ionizing Radiation. PLoS ONE. 2016;11:e0152213 pubmed publisher
  16. Kong F, Wang H, Guo J, Peng M, Ji H, Yang H, et al. Hsp70 suppresses apoptosis of BRL cells by regulating the expression of Bcl-2, cytochrome C, and caspase 8/3. In Vitro Cell Dev Biol Anim. 2016;52:568-75 pubmed publisher
  17. Peng C, Kaščáková S, Chiappini F, Olaya N, Sandt C, Yousef I, et al. Discrimination of cirrhotic nodules, dysplastic lesions and hepatocellular carcinoma by their vibrational signature. J Transl Med. 2016;14:9 pubmed publisher
  18. Roundhill E, Turnbull D, Burchill S. Localization of MRP-1 to the outer mitochondrial membrane by the chaperone protein HSP90β. FASEB J. 2016;30:1712-23 pubmed publisher
  19. Tang S, Chen H, Cheng Y, Nasir M, Kemper N, Bao E. The interactive association between heat shock factor 1 and heat shock proteins in primary myocardial cells subjected to heat stress. Int J Mol Med. 2016;37:56-62 pubmed publisher
  20. Toni L, Padilla P. Developmentally arrested Austrofundulus limnaeus embryos have changes in post-translational modifications of histone H3. J Exp Biol. 2016;219:544-52 pubmed publisher
  21. Schachtner H, Weimershaus M, Stache V, Plewa N, Legler D, Höpken U, et al. Loss of Gadkin Affects Dendritic Cell Migration In Vitro. PLoS ONE. 2015;10:e0143883 pubmed publisher
  22. Lima F, Gomes F, Seabra R, Wethey D, Seabra M, Cruz T, et al. Loss of thermal refugia near equatorial range limits. Glob Chang Biol. 2016;22:254-63 pubmed publisher
  23. Aleng N, Sung Y, MacRae T, Abd Wahid M. Non-Lethal Heat Shock of the Asian Green Mussel, Perna viridis, Promotes Hsp70 Synthesis, Induces Thermotolerance and Protects Against Vibrio Infection. PLoS ONE. 2015;10:e0135603 pubmed publisher
  24. Xu D, Sun L, Liu S, Zhang L, Yang H. Histological, ultrastructural and heat shock protein 70 (HSP70) responses to heat stress in the sea cucumber Apostichopus japonicus. Fish Shellfish Immunol. 2015;45:321-6 pubmed publisher
  25. Azimzadeh O, Sievert W, Sarioglu H, Merl Pham J, Yentrapalli R, Bakshi M, et al. Integrative proteomics and targeted transcriptomics analyses in cardiac endothelial cells unravel mechanisms of long-term radiation-induced vascular dysfunction. J Proteome Res. 2015;14:1203-19 pubmed publisher
  26. Cho O, Mallappa C, Hernández Hernández J, Rivera Pérez J, Imbalzano A. Contrasting roles for MyoD in organizing myogenic promoter structures during embryonic skeletal muscle development. Dev Dyn. 2015;244:43-55 pubmed publisher
  27. Nuss J, Kehn Hall K, Benedict A, Costantino J, Ward M, Peyser B, et al. Multi-faceted proteomic characterization of host protein complement of Rift Valley fever virus virions and identification of specific heat shock proteins, including HSP90, as important viral host factors. PLoS ONE. 2014;9:e93483 pubmed publisher
  28. Qi L, Zhang X, Wu J, Lin F, Wang J, DiFiglia M, et al. The role of chaperone-mediated autophagy in huntingtin degradation. PLoS ONE. 2012;7:e46834 pubmed publisher
  29. Calabria G, Dolgova O, Rego C, Castañeda L, Rezende E, Balanya J, et al. Hsp70 protein levels and thermotolerance in Drosophila subobscura: a reassessment of the thermal co-adaptation hypothesis. J Evol Biol. 2012;25:691-700 pubmed publisher
  30. Fischer K, Kölzow N, Höltje H, Karl I. Assay conditions in laboratory experiments: is the use of constant rather than fluctuating temperatures justified when investigating temperature-induced plasticity?. Oecologia. 2011;166:23-33 pubmed publisher
  31. Blank M, Bastrop R, Jürss K. Stress protein response in two sibling species of Marenzelleria (Polychaeta: Spionidae): is there an influence of acclimation salinity?. Comp Biochem Physiol B Biochem Mol Biol. 2006;144:451-62 pubmed
  32. Krauss M, Haucke V. Functional assay of effectors of ADP ribosylation factor 6 during clathrin/AP-2 coat recruitment to membranes. Methods Enzymol. 2005;404:388-98 pubmed