This is a Validated Antibody Database (VAD) review about dogs SPP1, based on 30 published articles (read how Labome selects the articles), using SPP1 antibody in all methods. It is aimed to help Labome visitors find the most suited SPP1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Abcam
domestic rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig 6b
Abcam SPP1 antibody (Abcam, ab8448) was used in western blot knockout validation on human samples (fig 6b). elife (2021) ncbi
Abcam
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:500; fig s2g
Abcam SPP1 antibody (Abcam, ab8448) was used in immunocytochemistry on mouse samples at 1:500 (fig s2g). Int J Oral Sci (2022) ncbi
domestic rabbit polyclonal
  • western blot; rat; 1:1000; loading ...; fig 2e
Abcam SPP1 antibody (Abcam, ab8448) was used in western blot on rat samples at 1:1000 (fig 2e). Aging (Albany NY) (2021) ncbi
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 3b
Abcam SPP1 antibody (Abcam, ab8448) was used in western blot on rat samples (fig 3b). Oxid Med Cell Longev (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig s1b
Abcam SPP1 antibody (abcam, ab8448) was used in western blot on human samples (fig s1b). BMC Cancer (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4c
Abcam SPP1 antibody (Abcam, ab8448) was used in western blot on human samples (fig 4c). Aging (Albany NY) (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:50; loading ...; fig 3b
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry on mouse samples at 1:50 (fig 3b). Bone Res (2021) ncbi
domestic rabbit polyclonal
  • western blot knockout validation; human; loading ...; fig 6b
Abcam SPP1 antibody (Abcam, ab8448) was used in western blot knockout validation on human samples (fig 6b). elife (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 5a
Abcam SPP1 antibody (Abcam, ab8448) was used in western blot on mouse samples at 1:1000 (fig 5a). J Clin Invest (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; loading ...
Abcam SPP1 antibody (abcam, ab8448) was used in immunohistochemistry on mouse samples . Nat Commun (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 4d
Abcam SPP1 antibody (Abcam, Ab8448) was used in western blot on human samples (fig 4d). Front Cell Dev Biol (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 4b
Abcam SPP1 antibody (Abcam, 8448) was used in western blot on mouse samples (fig 4b). Bioact Mater (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; loading ...; fig 4h
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on rat samples (fig 4h). PLoS ONE (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; fig 3d
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry on mouse samples (fig 3d). Oxid Med Cell Longev (2021) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 5e
Abcam SPP1 antibody (Abcam, ab8448) was used in western blot on mouse samples (fig 5e). J Cell Mol Med (2021) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 2d
  • western blot; mouse; loading ...; fig 7d, 7h
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on mouse samples (fig 2d) and in western blot on mouse samples (fig 7d, 7h). PLoS Genet (2021) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:1000; loading ...; fig 2a
Abcam SPP1 antibody (Abcam, ab8448) was used in immunocytochemistry on human samples at 1:1000 (fig 2a). Sci Adv (2020) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; rat; 1:500; loading ...; fig 2a
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry on rat samples at 1:500 (fig 2a). Front Cell Neurosci (2019) ncbi
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 4b
Abcam SPP1 antibody (Abcam, ab8448) was used in western blot on rat samples (fig 4b). Biosci Rep (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1a
Abcam SPP1 antibody (Abcam, ab8448) was used in western blot on human samples at 1:1000 (fig 1a). Biomed Pharmacother (2019) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:200; loading ...; fig 5h
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - frozen section on human samples at 1:200 (fig 5h). J Histochem Cytochem (2018) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 1f
  • western blot; human; fig 1b
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 1f) and in western blot on human samples (fig 1b). PLoS ONE (2017) ncbi
domestic rabbit polyclonal
  • flow cytometry; rat; loading ...; fig 7c
  • western blot; rat; loading ...; fig 3c
Abcam SPP1 antibody (Abcam, ab8448) was used in flow cytometry on rat samples (fig 7c) and in western blot on rat samples (fig 3c). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - frozen section; human; fig 6
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - frozen section on human samples (fig 6). Front Physiol (2016) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000
In order to study the role of kif3a in dental mesenchymal stem and precursor cell differentiation, Abcam SPP1 antibody (Abcam, ab8448) was used in western blot on human samples at 1:1000. Mol Med Rep (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:500; fig 5
Abcam SPP1 antibody (abcam, ab8448) was used in western blot on mouse samples at 1:500 (fig 5). Nat Commun (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; fig 5b
In order to analyze NFATc1 and its promotion of prostate tumorigenesis and its ability to overcome PTEN loss-induced senescence, Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry on mouse samples at 1:100 (fig 5b). Oncogene (2016) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (MPIIIB10(1))
  • immunohistochemistry; chicken; fig 4
Developmental Studies Hybridoma Bank SPP1 antibody (Developmental Studies Hybridoma Bank, MPIIIB10) was used in immunohistochemistry on chicken samples (fig 4). Front Neuroanat (2016) ncbi
mouse monoclonal (MPIIIB10(1))
  • immunocytochemistry; mouse; 1:2000; fig 2
  • western blot; mouse; 1:50,000; fig 5f
In order to characterize intramembranous and endochondral ossification peroxisomes in different skeletal cell types and regulation of osteoblast differentiation by distinct peroxisome proliferator-activated receptors, Developmental Studies Hybridoma Bank SPP1 antibody (Developmental Studies Hybridoma Bank (DSHB), MPIIIB101) was used in immunocytochemistry on mouse samples at 1:2000 (fig 2) and in western blot on mouse samples at 1:50,000 (fig 5f). PLoS ONE (2015) ncbi
mouse monoclonal (MPIIIB10(1))
  • immunohistochemistry - paraffin section; rat; 1:300; fig 3
Developmental Studies Hybridoma Bank SPP1 antibody (Developmental Hybridoma Studies Institute, MPIIIB10) was used in immunohistochemistry - paraffin section on rat samples at 1:300 (fig 3). PLoS ONE (2015) ncbi
mouse monoclonal (MPIIIB10(1))
  • immunocytochemistry; human; 1:5
Developmental Studies Hybridoma Bank SPP1 antibody (DSHB, MPIIIB10) was used in immunocytochemistry on human samples at 1:5. Anticancer Res (2004) ncbi
Articles Reviewed
  1. Chen Z, Wu W, Zheng C, Lan Y, Xie H, Xie Z. KLF6 facilitates differentiation of odontoblasts through modulating the expression of P21 in vitro. Int J Oral Sci. 2022;14:20 pubmed publisher
  2. Han H, Tian T, Huang G, Li D, Yang S. The lncRNA H19/miR-541-3p/Wnt/β-catenin axis plays a vital role in melatonin-mediated osteogenic differentiation of bone marrow mesenchymal stem cells. Aging (Albany NY). 2021;13:18257-18273 pubmed publisher
  3. Wu Y, Zhang J, Li C, Hu H, Qin B, Wang T, et al. The Activation of ROS/NF-κB/MMP-9 Pathway Promotes Calcium-Induced Kidney Crystal Deposition. Oxid Med Cell Longev. 2021;2021:8836355 pubmed publisher
  4. Huang J, Hu M, Niu H, Wang J, Si Y, Cheng S, et al. Osteopontin isoform c promotes the survival of cisplatin-treated NSCLC cells involving NFATc2-mediated suppression on calcium-induced ROS levels. BMC Cancer. 2021;21:750 pubmed publisher
  5. Huang S, Luo W, Wu G, Shen Q, Zhuang Z, Yang D, et al. Inhibition of CDK9 attenuates atherosclerosis by inhibiting inflammation and phenotypic switching of vascular smooth muscle cells. Aging (Albany NY). 2021;13:14892-14909 pubmed publisher
  6. Wang C, Ying J, Nie X, Zhou T, Xiao D, Swarnkar G, et al. Targeting angiogenesis for fracture nonunion treatment in inflammatory disease. Bone Res. 2021;9:29 pubmed publisher
  7. Hendley A, Rao A, Leonhardt L, Ashe S, Smith J, Giacometti S, et al. Single-cell transcriptome analysis defines heterogeneity of the murine pancreatic ductal tree. elife. 2021;10: pubmed publisher
  8. Ouyang L, Su X, Li W, Tang L, Zhang M, Zhu Y, et al. ALKBH1-demethylated DNA N6-methyladenine modification triggers vascular calcification via osteogenic reprogramming in chronic kidney disease. J Clin Invest. 2021;131: pubmed publisher
  9. Tsutsui K, Machida H, Nakagawa A, Ahn K, Morita R, Sekiguchi K, et al. Mapping the molecular and structural specialization of the skin basement membrane for inter-tissue interactions. Nat Commun. 2021;12:2577 pubmed publisher
  10. Chen Q, Liu X, Wang D, Zheng J, Chen L, Xie Q, et al. Periodontal Inflammation-Triggered by Periodontal Ligament Stem Cell Pyroptosis Exacerbates Periodontitis. Front Cell Dev Biol. 2021;9:663037 pubmed publisher
  11. Huang J, Li R, Yang J, Cai M, Lee Y, Wang A, et al. Bioadaptation of implants to In vitro and In vivo oxidative stress pathological conditions via nanotopography-induced FoxO1 signaling pathways to enhance Osteoimmunal regeneration. Bioact Mater. 2021;6:3164-3176 pubmed publisher
  12. Brito V, Patrocinio M, Sousa M, Barreto A, Frasnelli S, Lara V, et al. Mast cells contribute to alveolar bone loss in Spontaneously Hypertensive Rats with periodontal disease regulating cytokines production. PLoS ONE. 2021;16:e0247372 pubmed publisher
  13. Zhang Y, Yang Y, Xu M, Zheng J, Xu Y, Chen G, et al. The Dual Effects of Reactive Oxygen Species on the Mandibular Alveolar Bone Formation in SOD1 Knockout Mice: Promotion or Inhibition. Oxid Med Cell Longev. 2021;2021:8847140 pubmed publisher
  14. Wang Z, Cheng J, Liu B, Xie F, Li C, Qiao W, et al. Protein deglycase DJ-1 deficiency induces phenotypic switching in vascular smooth muscle cells and exacerbates atherosclerotic plaque instability. J Cell Mol Med. 2021;25:2816-2827 pubmed publisher
  15. Tang C, Wu M, Zhao D, Edwards D, McVicar A, Luo Y, et al. Runx1 is a central regulator of osteogenesis for bone homeostasis by orchestrating BMP and WNT signaling pathways. PLoS Genet. 2021;17:e1009233 pubmed publisher
  16. Barney L, Hall C, Schwartz A, Parks A, Sparages C, Galarza S, et al. Tumor cell-organized fibronectin maintenance of a dormant breast cancer population. Sci Adv. 2020;6:eaaz4157 pubmed publisher
  17. Rabenstein M, Unverricht Yeboah M, Keuters M, Pikhovych A, Hucklenbroich J, Vay S, et al. Transcranial Current Stimulation Alters the Expression of Immune-Mediating Genes. Front Cell Neurosci. 2019;13:461 pubmed publisher
  18. Fang G, Qi J, Huang L, Zhao X. LncRNA MRAK048635_P1 is critical for vascular smooth muscle cell function and phenotypic switching in essential hypertension. Biosci Rep. 2019;: pubmed publisher
  19. He S, Nian F, Chen W, Yin L, Auchoybur M, Tao Z, et al. I-κB kinase-ε knockout protects against angiotensin II induced aortic valve thickening in apolipoprotein E deficient mice. Biomed Pharmacother. 2019;109:1287-1295 pubmed publisher
  20. Hira V, Wormer J, Kakar H, Breznik B, van der Swaan B, Hulsbos R, et al. Periarteriolar Glioblastoma Stem Cell Niches Express Bone Marrow Hematopoietic Stem Cell Niche Proteins. J Histochem Cytochem. 2018;66:155-173 pubmed publisher
  21. Dickerson M, Vierra N, Milian S, Dadi P, Jacobson D. Osteopontin activates the diabetes-associated potassium channel TALK-1 in pancreatic β-cells. PLoS ONE. 2017;12:e0175069 pubmed publisher
  22. Feng W, Zhang K, Liu Y, Chen J, Cai Q, Zhang Y, et al. Apocynin attenuates angiotensin II-induced vascular smooth muscle cells osteogenic switching via suppressing extracellular signal-regulated kinase 1/2. Oncotarget. 2016;7:83588-83600 pubmed publisher
  23. Stanic K, Saldivia N, Förstera B, Torrejon M, Montecinos H, Caprile T. Expression Patterns of Extracellular Matrix Proteins during Posterior Commissure Development. Front Neuroanat. 2016;10:89 pubmed
  24. Spina A, Montella R, Liccardo D, De Rosa A, Laino L, Mitsiadis T, et al. NZ-GMP Approved Serum Improve hDPSC Osteogenic Commitment and Increase Angiogenic Factor Expression. Front Physiol. 2016;7:354 pubmed publisher
  25. Jiang S, Chen G, Feng L, Jiang Z, Yu M, Bao J, et al. Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway. Mol Med Rep. 2016;14:1891-900 pubmed publisher
  26. Yan B, Zhang Z, Jin D, Cai C, Jia C, Liu W, et al. mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation. Nat Commun. 2016;7:11151 pubmed publisher
  27. Qian G, Fan W, Ahlemeyer B, Karnati S, Baumgart Vogt E. Peroxisomes in Different Skeletal Cell Types during Intramembranous and Endochondral Ossification and Their Regulation during Osteoblast Differentiation by Distinct Peroxisome Proliferator-Activated Receptors. PLoS ONE. 2015;10:e0143439 pubmed publisher
  28. Manda K, Tripathi P, Hsi A, Ning J, Ruzinova M, Liapis H, et al. NFATc1 promotes prostate tumorigenesis and overcomes PTEN loss-induced senescence. Oncogene. 2016;35:3282-92 pubmed publisher
  29. Frenay A, Yazdani S, Boersema M, van der Graaf A, Waanders F, van den Born J, et al. Incomplete Restoration of Angiotensin II-Induced Renal Extracellular Matrix Deposition and Inflammation Despite Complete Functional Recovery in Rats. PLoS ONE. 2015;10:e0129732 pubmed publisher
  30. Pautke C, Schieker M, Tischer T, Kolk A, Neth P, Mutschler W, et al. Characterization of osteosarcoma cell lines MG-63, Saos-2 and U-2 OS in comparison to human osteoblasts. Anticancer Res. 2004;24:3743-8 pubmed