This is a Validated Antibody Database (VAD) review about dog STMN1, based on 17 published articles (read how Labome selects the articles), using STMN1 antibody in all methods. It is aimed to help Labome visitors find the most suited STMN1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
STMN1 synonym: stathmin; stathmin 1/oncoprotein 18

Knockout validation
BD Biosciences
mouse monoclonal (8/SMN)
  • western blot knockout validation; mouse; 1:4000; loading ...; fig 3f
In order to determine the efficacy of an adeno-associated viral vector expressing a codon-optimized human SMN1 coding sequence under the control of the constitutive phosphoglycerate kinase promoter in a mouse model of spinal muscular atrophy, BD Biosciences STMN1 antibody (BD, 610646) was used in western blot knockout validation on mouse samples at 1:4000 (fig 3f). Mol Ther Methods Clin Dev (2016) ncbi
BD Biosciences
mouse monoclonal (8/SMN)
  • immunocytochemistry; human; loading ...; fig 1d, 3f
  • western blot; human; 1:100; loading ...; fig 1a, 3g
  • immunocytochemistry; mouse; fig 6a
BD Biosciences STMN1 antibody (BD, 610647) was used in immunocytochemistry on human samples (fig 1d, 3f), in western blot on human samples at 1:100 (fig 1a, 3g) and in immunocytochemistry on mouse samples (fig 6a). Nucleic Acids Res (2018) ncbi
mouse monoclonal (8/SMN)
  • western blot; mouse; 1:4000; loading ...; fig 10a
In order to clarify how actin isoforms modulate the axons of developing motoneurons, BD Biosciences STMN1 antibody (BD, 610646) was used in western blot on mouse samples at 1:4000 (fig 10a). J Cell Biol (2017) ncbi
mouse monoclonal (8/SMN)
  • western blot; human; loading ...; fig 1a
In order to assess the role of individual Survival of MotoNeuron proteins and methylosome components in selenoprotein mRNP assembly and translation, BD Biosciences STMN1 antibody (BD Transduction lab, 610646) was used in western blot on human samples (fig 1a). Nucleic Acids Res (2017) ncbi
mouse monoclonal (8/SMN)
  • western blot; human; 1:1000; loading ...; fig 1a
  • western blot; mouse; 1:1000; loading ...; fig 7e
  • western blot; zebrafish ; 1:1000; loading ...; fig 1d
In order to discuss targeting UBA1 as a means to treat spinal muscular atrophy, BD Biosciences STMN1 antibody (BD Biosciences, 610646) was used in western blot on human samples at 1:1000 (fig 1a), in western blot on mouse samples at 1:1000 (fig 7e) and in western blot on zebrafish samples at 1:1000 (fig 1d). JCI Insight (2016) ncbi
mouse monoclonal (8/SMN)
  • western blot knockout validation; mouse; 1:4000; loading ...; fig 3f
In order to determine the efficacy of an adeno-associated viral vector expressing a codon-optimized human SMN1 coding sequence under the control of the constitutive phosphoglycerate kinase promoter in a mouse model of spinal muscular atrophy, BD Biosciences STMN1 antibody (BD, 610646) was used in western blot knockout validation on mouse samples at 1:4000 (fig 3f). Mol Ther Methods Clin Dev (2016) ncbi
mouse monoclonal (8/SMN)
  • western blot; mouse; 1:300; loading ...; fig 2c
  • immunocytochemistry; human; 1:5000; loading ...; fig 3c
In order to test if Morpholino-modified antisense oligonucleotide alter SMN2 splicing, BD Biosciences STMN1 antibody (BD Bio, 610646) was used in western blot on mouse samples at 1:300 (fig 2c) and in immunocytochemistry on human samples at 1:5000 (fig 3c). Mol Ther (2016) ncbi
mouse monoclonal (8/SMN)
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 4c
In order to test if neuronal loss is caused by toxic gain-of-function cytoplasmic aggregates or loss of nuclear RNA-binding protein function in patients with sporadic and familial amyotrophic lateral sclerosis, BD Biosciences STMN1 antibody (BD Bioscience, 610646) was used in immunohistochemistry on mouse samples at 1:1000 (fig 4c). Brain (2016) ncbi
mouse monoclonal (8/SMN)
  • western blot; human; fig 1
BD Biosciences STMN1 antibody (BD Transduction Laboratories, 610646) was used in western blot on human samples (fig 1). Biol Open (2016) ncbi
mouse monoclonal (8/SMN)
  • western blot; mouse; 1:2000; fig 6a
BD Biosciences STMN1 antibody (BD Transduction Laboratories, 610646) was used in western blot on mouse samples at 1:2000 (fig 6a). PLoS ONE (2016) ncbi
mouse monoclonal (8/SMN)
  • western blot; mouse; 1:2000; fig 3
BD Biosciences STMN1 antibody (BD Biosciences, 610646) was used in western blot on mouse samples at 1:2000 (fig 3). Sci Rep (2016) ncbi
mouse monoclonal (8/SMN)
  • western blot; human; loading ...; fig 1a
BD Biosciences STMN1 antibody (BD transaction laboratory, 8/SMN) was used in western blot on human samples (fig 1a). Nat Struct Mol Biol (2016) ncbi
mouse monoclonal (8/SMN)
  • western blot; mouse; 1:1000
BD Biosciences STMN1 antibody (BD Biosciences, 610647) was used in western blot on mouse samples at 1:1000. Hum Mol Genet (2015) ncbi
mouse monoclonal (8/SMN)
  • western blot; human; 1:5000; fig 1
In order to study how altered association with SMN and U1-snRNP can cause gain and loss of function by ALS-causative mutations in FUS/TLS, BD Biosciences STMN1 antibody (BD, 610646) was used in western blot on human samples at 1:5000 (fig 1). Nat Commun (2015) ncbi
mouse monoclonal (8/SMN)
  • immunocytochemistry; mouse; 1:2000; fig 5
  • western blot; mouse; 1:2000; fig 5
In order to report that Tat-activating regulatory DNA-binding protein-43 regulates the synthesis of the interleukin-6 and -10 splicing activating compartment, BD Biosciences STMN1 antibody (BD Biosciences, 610646) was used in immunocytochemistry on mouse samples at 1:2000 (fig 5) and in western blot on mouse samples at 1:2000 (fig 5). Nat Commun (2015) ncbi
mouse monoclonal (8/SMN)
  • western blot; rat; 1:5000
BD Biosciences STMN1 antibody (BD Biosciences, 610647) was used in western blot on rat samples at 1:5000. J Biol Chem (2014) ncbi
mouse monoclonal (8/SMN)
  • immunohistochemistry - paraffin section; human; fig 3
  • western blot; human; fig 2
In order to study the role of loss of spliceosome integrity in the development of amyotrophic lateral sclerosis and spinal muscular atrophy, BD Biosciences STMN1 antibody (BD transduction, 610646) was used in immunohistochemistry - paraffin section on human samples (fig 3) and in western blot on human samples (fig 2). EMBO Mol Med (2013) ncbi
mouse monoclonal (8/SMN)
  • immunocytochemistry; human; loading ...; fig 5
  • western blot; human; loading ...; fig 3
  • western blot; mouse; loading ...; fig 4
In order to examine the contribution of Gemin8 to the composition and function of the survival motor neuron protein complex, BD Biosciences STMN1 antibody (BD, 8) was used in immunocytochemistry on human samples (fig 5), in western blot on human samples (fig 3) and in western blot on mouse samples (fig 4). J Biol Chem (2006) ncbi
Articles Reviewed
  1. Kannan A, Bhatia K, Branzei D, Gangwani L. Combined deficiency of Senataxin and DNA-PKcs causes DNA damage accumulation and neurodegeneration in spinal muscular atrophy. Nucleic Acids Res. 2018;46:8326-8346 pubmed publisher
  2. Moradi M, Sivadasan R, Saal L, Lüningschrör P, Dombert B, Rathod R, et al. Differential roles of α-, β-, and γ-actin in axon growth and collateral branch formation in motoneurons. J Cell Biol. 2017;216:793-814 pubmed publisher
  3. Gribling Burrer A, Leichter M, Wurth L, Huttin A, Schlotter F, Troffer Charlier N, et al. SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation. Nucleic Acids Res. 2017;45:5399-5413 pubmed publisher
  4. Powis R, Karyka E, Boyd P, Côme J, Jones R, Zheng Y, et al. Systemic restoration of UBA1 ameliorates disease in spinal muscular atrophy. JCI Insight. 2016;1:e87908 pubmed
  5. Armbruster N, Lattanzi A, Jeavons M, Van Wittenberghe L, Gjata B, Marais T, et al. Efficacy and biodistribution analysis of intracerebroventricular administration of an optimized scAAV9-SMN1 vector in a mouse model of spinal muscular atrophy. Mol Ther Methods Clin Dev. 2016;3:16060 pubmed publisher
  6. Osman E, Washington C, Kaifer K, Mazzasette C, Patitucci T, Florea K, et al. Optimization of Morpholino Antisense Oligonucleotides Targeting the Intronic Repressor Element1 in Spinal Muscular Atrophy. Mol Ther. 2016;24:1592-601 pubmed publisher
  7. Shiihashi G, Ito D, Yagi T, Nihei Y, Ebine T, Suzuki N. Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice. Brain. 2016;139:2380-94 pubmed publisher
  8. Poole A, Hebert M. SMN and coilin negatively regulate dyskerin association with telomerase RNA. Biol Open. 2016;5:726-35 pubmed publisher
  9. Seo J, Singh N, Ottesen E, Sivanesan S, Shishimorova M, Singh R. Oxidative Stress Triggers Body-Wide Skipping of Multiple Exons of the Spinal Muscular Atrophy Gene. PLoS ONE. 2016;11:e0154390 pubmed publisher
  10. Ottesen E, Howell M, Singh N, Seo J, Whitley E, Singh R. Severe impairment of male reproductive organ development in a low SMN expressing mouse model of spinal muscular atrophy. Sci Rep. 2016;6:20193 pubmed publisher
  11. So B, Wan L, Zhang Z, Li P, Babiash E, Duan J, et al. A U1 snRNP-specific assembly pathway reveals the SMN complex as a versatile hub for RNP exchange. Nat Struct Mol Biol. 2016;23:225-30 pubmed publisher
  12. McGovern V, Iyer C, Arnold W, Gombash S, Zaworski P, Blatnik A, et al. SMN expression is required in motor neurons to rescue electrophysiological deficits in the SMNΔ7 mouse model of SMA. Hum Mol Genet. 2015;24:5524-41 pubmed publisher
  13. Sun S, Ling S, Qiu J, Albuquerque C, Zhou Y, Tokunaga S, et al. ALS-causative mutations in FUS/TLS confer gain and loss of function by altered association with SMN and U1-snRNP. Nat Commun. 2015;6:6171 pubmed publisher
  14. Lee S, Lee T, Lee E, Kang S, Park A, Kim S, et al. Identification of a subnuclear body involved in sequence-specific cytokine RNA processing. Nat Commun. 2015;6:5791 pubmed publisher
  15. Singh R, Brewer M, Mashburn C, Lou D, Bondada V, Graham B, et al. Calpain 5 is highly expressed in the central nervous system (CNS), carries dual nuclear localization signals, and is associated with nuclear promyelocytic leukemia protein bodies. J Biol Chem. 2014;289:19383-94 pubmed publisher
  16. Tsuiji H, Iguchi Y, Furuya A, Kataoka A, Hatsuta H, Atsuta N, et al. Spliceosome integrity is defective in the motor neuron diseases ALS and SMA. EMBO Mol Med. 2013;5:221-34 pubmed publisher
  17. Carissimi C, Saieva L, Gabanella F, Pellizzoni L. Gemin8 is required for the architecture and function of the survival motor neuron complex. J Biol Chem. 2006;281:37009-16 pubmed