This is a Validated Antibody Database (VAD) review about dog SPP1, based on 19 published articles (read how Labome selects the articles), using SPP1 antibody in all methods. It is aimed to help Labome visitors find the most suited SPP1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SPP1 synonym: osteopontin; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)

Abcam
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1a
Abcam SPP1 antibody (Abcam, ab8448) was used in western blot on human samples at 1:1000 (fig 1a). Biomed Pharmacother (2019) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; human; 1:200; loading ...; fig 5h
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - frozen section on human samples at 1:200 (fig 5h). J Histochem Cytochem (2018) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 1f
  • western blot; human; fig 1b
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 1f) and in western blot on human samples (fig 1b). PLoS ONE (2017) ncbi
rabbit polyclonal
  • flow cytometry; rat; loading ...; fig 7c
  • western blot; rat; loading ...; fig 3c
Abcam SPP1 antibody (Abcam, ab8448) was used in flow cytometry on rat samples (fig 7c) and in western blot on rat samples (fig 3c). Oncotarget (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; human; fig 6
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - frozen section on human samples (fig 6). Front Physiol (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000
In order to study the role of kif3a in dental mesenchymal stem and precursor cell differentiation, Abcam SPP1 antibody (Abcam, ab8448) was used in western blot on human samples at 1:1000. Mol Med Rep (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:500; fig 5
Abcam SPP1 antibody (abcam, ab8448) was used in western blot on mouse samples at 1:500 (fig 5). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:100; fig 5b
In order to analyze NFATc1 and its promotion of prostate tumorigenesis and its ability to overcome PTEN loss-induced senescence, Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry on mouse samples at 1:100 (fig 5b). Oncogene (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 6
Abcam SPP1 antibody (Abcam, ab8448) was used in western blot on human samples at 1:1000 (fig 6). PLoS ONE (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:200; fig 5e,5g
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry on rat samples at 1:200 (fig 5e,5g). Mol Med Rep (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on mouse samples . J Mol Cell Cardiol (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200
In order to investigate the role of WDC1 during infection and wound healing using wdc1-null mice, Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry on mouse samples at 1:200. Am J Pathol (2014) ncbi
rabbit polyclonal
  • western blot; mouse
Abcam SPP1 antibody (Abcam, ab8448) was used in western blot on mouse samples . J Cell Mol Med (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; fig s2
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on mouse samples (fig s2). Bone (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; 1:300
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on rat samples at 1:300. Urology (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human
Abcam SPP1 antibody (Abcam, ab8448) was used in immunocytochemistry on human samples . J Biomed Mater Res A (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100
  • western blot; mouse; 1:1000
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 and in western blot on mouse samples at 1:1000. J Biomed Mater Res B Appl Biomater (2013) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:400
Abcam SPP1 antibody (Abcam, ab8448) was used in immunohistochemistry - paraffin section on mouse samples at 1:400. J Cell Physiol (2013) ncbi
Rockland Immunochemicals
rabbit polyclonal
  • western blot; mouse; loading ...; fig s8c
In order to research the atheroprotective effect of OCT4 in smooth muscle cells, Rockland Immunochemicals SPP1 antibody (Rockland, 100-401-404) was used in western blot on mouse samples (fig s8c). Nat Med (2016) ncbi
Articles Reviewed
  1. He S, Nian F, Chen W, Yin L, Auchoybur M, Tao Z, et al. I-κB kinase-ε knockout protects against angiotensin II induced aortic valve thickening in apolipoprotein E deficient mice. Biomed Pharmacother. 2019;109:1287-1295 pubmed publisher
  2. Hira V, Wormer J, Kakar H, Breznik B, van der Swaan B, Hulsbos R, et al. Periarteriolar Glioblastoma Stem Cell Niches Express Bone Marrow Hematopoietic Stem Cell Niche Proteins. J Histochem Cytochem. 2018;66:155-173 pubmed publisher
  3. Dickerson M, Vierra N, Milian S, Dadi P, Jacobson D. Osteopontin activates the diabetes-associated potassium channel TALK-1 in pancreatic β-cells. PLoS ONE. 2017;12:e0175069 pubmed publisher
  4. Feng W, Zhang K, Liu Y, Chen J, Cai Q, Zhang Y, et al. Apocynin attenuates angiotensin II-induced vascular smooth muscle cells osteogenic switching via suppressing extracellular signal-regulated kinase 1/2. Oncotarget. 2016;7:83588-83600 pubmed publisher
  5. Spina A, Montella R, Liccardo D, De Rosa A, Laino L, Mitsiadis T, et al. NZ-GMP Approved Serum Improve hDPSC Osteogenic Commitment and Increase Angiogenic Factor Expression. Front Physiol. 2016;7:354 pubmed publisher
  6. Jiang S, Chen G, Feng L, Jiang Z, Yu M, Bao J, et al. Disruption of kif3a results in defective osteoblastic differentiation in dental mesenchymal stem/precursor cells via the Wnt signaling pathway. Mol Med Rep. 2016;14:1891-900 pubmed publisher
  7. Cherepanova O, Gomez D, Shankman L, Swiatlowska P, Williams J, Sarmento O, et al. Activation of the pluripotency factor OCT4 in smooth muscle cells is atheroprotective. Nat Med. 2016;22:657-65 pubmed publisher
  8. Yan B, Zhang Z, Jin D, Cai C, Jia C, Liu W, et al. mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation. Nat Commun. 2016;7:11151 pubmed publisher
  9. Manda K, Tripathi P, Hsi A, Ning J, Ruzinova M, Liapis H, et al. NFATc1 promotes prostate tumorigenesis and overcomes PTEN loss-induced senescence. Oncogene. 2016;35:3282-92 pubmed publisher
  10. Wu Y, Feng G, Song J, Zhang Y, Yu Y, Huang L, et al. TrAmplification of Human Dental Follicle Cells by piggyBac Transposon - Mediated Reversible Immortalization System. PLoS ONE. 2015;10:e0130937 pubmed publisher
  11. Liu Z, Liu L, Kang C, Xie Q, Zhang B, Li Y. Effects of estrogen deficiency on microstructural changes in rat alveolar bone proper and periodontal ligament. Mol Med Rep. 2015;12:3508-3514 pubmed publisher
  12. Seo K, Lee S, Ye B, Kim Y, Bae S, Kim C. Mechanical stretch enhances the expression and activity of osteopontin and MMP-2 via the Akt1/AP-1 pathways in VSMC. J Mol Cell Cardiol. 2015;85:13-24 pubmed publisher
  13. Ressler S, Dang T, Wu S, Tse D, Gilbert B, Vyakarnam A, et al. WFDC1 is a key modulator of inflammatory and wound repair responses. Am J Pathol. 2014;184:2951-64 pubmed publisher
  14. Su Z, Yin J, Wang T, Sun Y, Ni P, Ma R, et al. Up-regulated HMGB1 in EAM directly led to collagen deposition by a PKC?/Erk1/2-dependent pathway: cardiac fibroblast/myofibroblast might be another source of HMGB1. J Cell Mol Med. 2014;18:1740-51 pubmed publisher
  15. Wu M, Li C, Zhu G, Wang Y, Jules J, Lu Y, et al. Deletion of core-binding factor ? (Cbf?) in mesenchymal progenitor cells provides new insights into Cbf?/Runxs complex function in cartilage and bone development. Bone. 2014;65:49-59 pubmed publisher
  16. Jia Z, Wang S, Tang J, He D, Cui L, Liu Z, et al. Does crystal deposition in genetic hypercalciuric rat kidney tissue share similarities with bone formation?. Urology. 2014;83:509.e7-14 pubmed publisher
  17. Silva A, Paula A, Martins T, Goes A, Pereria M. Synergistic effect between bioactive glass foam and a perfusion bioreactor on osteogenic differentiation of human adipose stem cells. J Biomed Mater Res A. 2014;102:818-27 pubmed publisher
  18. Choi K, Kang B, Kim H, Lee S, Bae S, Kweon O, et al. Low-level laser therapy promotes the osteogenic potential of adipose-derived mesenchymal stem cells seeded on an acellular dermal matrix. J Biomed Mater Res B Appl Biomater. 2013;101:919-28 pubmed publisher
  19. Li S, Zhai Q, Zou D, Meng H, Xie Z, Li C, et al. A pivotal role of bone remodeling in granulocyte colony stimulating factor induced hematopoietic stem/progenitor cells mobilization. J Cell Physiol. 2013;228:1002-9 pubmed publisher