This is a Validated Antibody Database (VAD) review about dog SMAD2, based on 23 published articles (read how Labome selects the articles), using SMAD2 antibody in all methods. It is aimed to help Labome visitors find the most suited SMAD2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SMAD2 synonym: mothers against decapentaplegic homolog 2

BD Biosciences
mouse monoclonal (18/Smad2/3)
  • western blot; human; 1:1000; fig 1
  • western blot; mouse; 1:1000; fig s1
BD Biosciences SMAD2 antibody (BD, 610843) was used in western blot on human samples at 1:1000 (fig 1) and in western blot on mouse samples at 1:1000 (fig s1). Sci Rep (2016) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; mouse; 1:500; fig 6
BD Biosciences SMAD2 antibody (BD, 610843) was used in western blot on mouse samples at 1:500 (fig 6). Nat Commun (2016) ncbi
mouse monoclonal (18/Smad2/3)
  • ChIP-Seq; mouse; loading ...; fig 1a
BD Biosciences SMAD2 antibody (Primary Cell Signaling, 610843) was used in ChIP-Seq on mouse samples (fig 1a). PLoS ONE (2016) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; mouse; fig 4
In order to analyze regulation of NLRP3 inflammasome in adipose tissue by phosphodiesterase 3B (PDE3B), BD Biosciences SMAD2 antibody (BD Biosciences, 610843) was used in western blot on mouse samples (fig 4). Sci Rep (2016) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; human; 1:1000; fig 1
BD Biosciences SMAD2 antibody (BD, 610843) was used in western blot on human samples at 1:1000 (fig 1). EMBO Mol Med (2016) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; human; 1:1000; loading ...; fig 7h
In order to evaluate the ability of Chrdl1 to suppress bone morphogenetic protein-induced increases in breast cancer cell migration and invasion, BD Biosciences SMAD2 antibody (BD Biosciences, 610842) was used in western blot on human samples at 1:1000 (fig 7h). Mol Cell Biol (2016) ncbi
mouse monoclonal (18/Smad2/3)
  • chromatin immunoprecipitation; mouse; fig 1
  • western blot; mouse; fig 1
BD Biosciences SMAD2 antibody (BD Biosciences, 610842) was used in chromatin immunoprecipitation on mouse samples (fig 1) and in western blot on mouse samples (fig 1). Diabetes (2016) ncbi
mouse monoclonal (18/Smad2/3)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences SMAD2 antibody (BD, 18) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; mouse; 1:1000; fig 6
BD Biosciences SMAD2 antibody (BD, 610843) was used in western blot on mouse samples at 1:1000 (fig 6). Mol Med Rep (2016) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; mouse; 1:350
BD Biosciences SMAD2 antibody (BD Biosciences, 610842) was used in western blot on mouse samples at 1:350. Reprod Sci (2016) ncbi
mouse monoclonal (18/Smad2/3)
  • immunocytochemistry; human; 1:500
  • western blot; human; 1:1000
In order to show that tamoxifen prevents myofibroblast differentiation, BD Biosciences SMAD2 antibody (BD Biosciences, 610842) was used in immunocytochemistry on human samples at 1:500 and in western blot on human samples at 1:1000. J Cell Physiol (2015) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; human; 1:500; fig 5
BD Biosciences SMAD2 antibody (BD, 610,843) was used in western blot on human samples at 1:500 (fig 5). Nat Commun (2015) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; human; fig 4c
BD Biosciences SMAD2 antibody (BD, 610842) was used in western blot on human samples (fig 4c). Breast Cancer Res (2015) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; human; fig 2
BD Biosciences SMAD2 antibody (BD Biosciences, 610842) was used in western blot on human samples (fig 2). J Biol Chem (2015) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; mouse; fig s4
BD Biosciences SMAD2 antibody (BD Biosciences, BDB610842) was used in western blot on mouse samples (fig s4). Cell (2014) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; human
  • western blot; mouse; fig 2
BD Biosciences SMAD2 antibody (BD Biosciences, 610842) was used in western blot on human samples and in western blot on mouse samples (fig 2). J Biol Chem (2013) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; rat
  • western blot; mouse
BD Biosciences SMAD2 antibody (BD, 610842) was used in western blot on rat samples and in western blot on mouse samples . Am J Pathol (2012) ncbi
mouse monoclonal (18/Smad2/3)
  • western blot; mouse; 1:500
In order to study the role of TGF-beta inhibitors in abolishing the resistance of glioblastoma to ionozing radiation therapy, BD Biosciences SMAD2 antibody (BD Transduction Laboratories, 610842) was used in western blot on mouse samples at 1:500. Cancer Res (2012) ncbi
EMD Millipore
rabbit monoclonal (A5S)
  • western blot; mouse; loading ...; fig 1f
EMD Millipore SMAD2 antibody (Millipore, A5S) was used in western blot on mouse samples (fig 1f). J Clin Invest (2019) ncbi
rabbit monoclonal (A5S)
  • western blot; mouse; 1:1000; loading ...; fig 4a
EMD Millipore SMAD2 antibody (Millipore, 04-953) was used in western blot on mouse samples at 1:1000 (fig 4a). J Exp Med (2018) ncbi
rabbit monoclonal (A5S)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 4m
In order to discuss X-linked mutations that result in thoracic aortic aneurysm and dissection, EMD Millipore SMAD2 antibody (Millipore, 04-953) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 4m). Genet Med (2017) ncbi
rabbit monoclonal (A5S)
  • western blot; rat; 1:2500; loading ...; fig 1a
In order to test if nicotinamide adenosine diphosphate oxidase 2 contributes to cyclosporine A-induced chronic hypoxia, EMD Millipore SMAD2 antibody (Millipore, 04-953) was used in western blot on rat samples at 1:2500 (fig 1a). Transplantation (2016) ncbi
rabbit monoclonal (A5S)
  • western blot; mouse; 1:1000; fig 6
EMD Millipore SMAD2 antibody (Merck Millipore, 04-953) was used in western blot on mouse samples at 1:1000 (fig 6). Mol Med Rep (2016) ncbi
rabbit monoclonal (A5S)
  • western blot; human; 1:500; fig 5
EMD Millipore SMAD2 antibody (Millipore, 04-953) was used in western blot on human samples at 1:500 (fig 5). Nat Commun (2015) ncbi
rabbit monoclonal (A5S)
  • western blot; mouse; fig 5
  • western blot; rat
  • western blot; human
EMD Millipore SMAD2 antibody (Millipore, A5S) was used in western blot on mouse samples (fig 5), in western blot on rat samples and in western blot on human samples . Biol Cell (2013) ncbi
Articles Reviewed
  1. MacFarlane E, Parker S, Shin J, Kang B, Ziegler S, Creamer T, et al. Lineage-specific events underlie aortic root aneurysm pathogenesis in Loeys-Dietz syndrome. J Clin Invest. 2019;129:659-675 pubmed publisher
  2. Jiang L, Han X, Wang J, Wang C, Sun X, Xie J, et al. SHP-1 regulates hematopoietic stem cell quiescence by coordinating TGF-β signaling. J Exp Med. 2018;215:1337-1347 pubmed publisher
  3. Chen P, Qin L, Li G, Tellides G, Simons M. Fibroblast growth factor (FGF) signaling regulates transforming growth factor beta (TGF?)-dependent smooth muscle cell phenotype modulation. Sci Rep. 2016;6:33407 pubmed publisher
  4. Meester J, Vandeweyer G, Pintelon I, Lammens M, Van Hoorick L, De Belder S, et al. Loss-of-function mutations in the X-linked biglycan gene cause a severe syndromic form of thoracic aortic aneurysms and dissections. Genet Med. 2017;19:386-395 pubmed publisher
  5. Nakamichi R, Ito Y, Inui M, Onizuka N, Kayama T, Kataoka K, et al. Mohawk promotes the maintenance and regeneration of the outer annulus fibrosus of intervertebral discs. Nat Commun. 2016;7:12503 pubmed publisher
  6. Omata Y, Nakamura S, Koyama T, Yasui T, Hirose J, Izawa N, et al. Identification of Nedd9 as a TGF-?-Smad2/3 Target Gene Involved in RANKL-Induced Osteoclastogenesis by Comprehensive Analysis. PLoS ONE. 2016;11:e0157992 pubmed publisher
  7. Ahmad F, Chung Y, Tang Y, Hockman S, Liu S, Khan Y, et al. Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue. Sci Rep. 2016;6:28056 pubmed publisher
  8. Chen P, Qin L, Li G, Tellides G, Simons M. Smooth muscle FGF/TGFβ cross talk regulates atherosclerosis progression. EMBO Mol Med. 2016;8:712-28 pubmed publisher
  9. Cyr Depauw C, Northey J, Tabariès S, Annis M, Dong Z, Cory S, et al. Chordin-Like 1 Suppresses Bone Morphogenetic Protein 4-Induced Breast Cancer Cell Migration and Invasion. Mol Cell Biol. 2016;36:1509-25 pubmed publisher
  10. Djamali A, Wilson N, Sadowski E, Zha W, Niles D, Hafez O, et al. Nox2 and Cyclosporine-Induced Renal Hypoxia. Transplantation. 2016;100:1198-210 pubmed publisher
  11. Dhawan S, Dirice E, Kulkarni R, Bhushan A. Inhibition of TGF-β Signaling Promotes Human Pancreatic β-Cell Replication. Diabetes. 2016;65:1208-18 pubmed publisher
  12. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  13. Sawada S, Chosa N, Takizawa N, Yokota J, Igarashi Y, Tomoda K, et al. Establishment of mesenchymal stem cell lines derived from the bone marrow of green fluorescent protein-transgenic mice exhibiting a diversity in intracellular transforming growth factor-β and bone morphogenetic protein signaling. Mol Med Rep. 2016;13:2023-31 pubmed publisher
  14. Bacallao K, Plaza Parrochia F, Cerda A, Gabler F, Romero C, Vantman D, et al. Levels of Regulatory Proteins Associated With Cell Proliferation in Endometria From Untreated Patients Having Polycystic Ovarian Syndrome With and Without Endometrial Hyperplasia. Reprod Sci. 2016;23:211-8 pubmed publisher
  15. Carthy J, Sundqvist A, Heldin A, van Dam H, Kletsas D, Heldin C, et al. Tamoxifen Inhibits TGF-β-Mediated Activation of Myofibroblasts by Blocking Non-Smad Signaling Through ERK1/2. J Cell Physiol. 2015;230:3084-92 pubmed publisher
  16. Aspalter I, Gordon E, Dubrac A, Ragab A, Narloch J, Vizan P, et al. Alk1 and Alk5 inhibition by Nrp1 controls vascular sprouting downstream of Notch. Nat Commun. 2015;6:7264 pubmed publisher
  17. Li Y, Drabsch Y, Pujuguet P, Ren J, van Laar T, Zhang L, et al. Genetic depletion and pharmacological targeting of αv integrin in breast cancer cells impairs metastasis in zebrafish and mouse xenograft models. Breast Cancer Res. 2015;17:28 pubmed publisher
  18. Aki S, Yoshioka K, Okamoto Y, Takuwa N, Takuwa Y. Phosphatidylinositol 3-kinase class II α-isoform PI3K-C2α is required for transforming growth factor β-induced Smad signaling in endothelial cells. J Biol Chem. 2015;290:6086-105 pubmed publisher
  19. Scherz Shouval R, Santagata S, Mendillo M, Sholl L, Ben Aharon I, Beck A, et al. The reprogramming of tumor stroma by HSF1 is a potent enabler of malignancy. Cell. 2014;158:564-78 pubmed publisher
  20. Sakaki Yumoto M, Liu J, Ramalho Santos M, Yoshida N, Derynck R. Smad2 is essential for maintenance of the human and mouse primed pluripotent stem cell state. J Biol Chem. 2013;288:18546-60 pubmed publisher
  21. Juin A, Planus E, Guillemot F, Horáková P, Albiges Rizo C, Genot E, et al. Extracellular matrix rigidity controls podosome induction in microvascular endothelial cells. Biol Cell. 2013;105:46-57 pubmed publisher
  22. Geng H, Lan R, Singha P, Gilchrist A, Weinreb P, Violette S, et al. Lysophosphatidic acid increases proximal tubule cell secretion of profibrotic cytokines PDGF-B and CTGF through LPA2- and G?q-mediated Rho and ?v?6 integrin-dependent activation of TGF-?. Am J Pathol. 2012;181:1236-49 pubmed publisher
  23. Hardee M, Marciscano A, Medina Ramirez C, Zagzag D, Narayana A, Lonning S, et al. Resistance of glioblastoma-initiating cells to radiation mediated by the tumor microenvironment can be abolished by inhibiting transforming growth factor-?. Cancer Res. 2012;72:4119-29 pubmed publisher