This is a Validated Antibody Database (VAD) review about dog SLC12A5, based on 11 published articles (read how Labome selects the articles), using SLC12A5 antibody in all methods. It is aimed to help Labome visitors find the most suited SLC12A5 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SLC12A5 synonym: solute carrier family 12 member 5; K-Cl cotransporter 2; solute carrier family 12 (potassium-chloride transporter), member 5; solute carrier family 12, (potassium-chloride transporter) member 5

EMD Millipore
rabbit polyclonal
  • immunoprecipitation; rat; loading ...; fig 1C
  • immunocytochemistry; rat; loading ...; fig 1E
  • western blot; rat; loading ...; fig 1B
In order to report that activation of GABAB receptors results in a decrease in potassium-chloride cotransporter protein function, EMD Millipore SLC12A5 antibody (Millipore, 07-432) was used in immunoprecipitation on rat samples (fig 1C), in immunocytochemistry on rat samples (fig 1E) and in western blot on rat samples (fig 1B). J Neurosci (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 2-s1
In order to investigate how prostaglandin E2 regulates breathing in young mice, EMD Millipore SLC12A5 antibody (Millipore, 07-432) was used in immunohistochemistry on mouse samples (fig 2-s1). elife (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:1000; loading ...; fig 4g
In order to determine if medial or lateral habenula or their subnuclei display field-characteristic gene products, EMD Millipore SLC12A5 antibody (Millipore, 07-432) was used in immunohistochemistry on rat samples at 1:1000 (fig 4g). Brain Struct Funct (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:200; fig 4
  • western blot; rat; 1:1000; fig 1
In order to investigate the rescue of seizure susceptibility and spine morphology in atypical febrile seizures by reducing premature KCC2 expression, EMD Millipore SLC12A5 antibody (Millipore, 07-432) was used in immunohistochemistry on rat samples at 1:200 (fig 4) and in western blot on rat samples at 1:1000 (fig 1). Neurobiol Dis (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; rat; 1:2000
  • immunohistochemistry - frozen section; rat; 1:300
EMD Millipore SLC12A5 antibody (Millipore, 07-432) was used in immunohistochemistry - free floating section on rat samples at 1:2000 and in immunohistochemistry - frozen section on rat samples at 1:300. J Comp Neurol (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:500
  • western blot; mouse; 1:1000
EMD Millipore SLC12A5 antibody (Millipore, 07-432) was used in immunohistochemistry - frozen section on mouse samples at 1:500 and in western blot on mouse samples at 1:1000. PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000; loading ...; fig s1
EMD Millipore SLC12A5 antibody (Millipore, 07-432) was used in western blot on rat samples at 1:1000 (fig s1). Nat Neurosci (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:500
EMD Millipore SLC12A5 antibody (Millipore, 07-432) was used in immunohistochemistry - frozen section on mouse samples at 1:500. Front Neural Circuits (2014) ncbi
rabbit polyclonal
  • western blot; rat; 1:1000
EMD Millipore SLC12A5 antibody (Millipore, 07-432) was used in western blot on rat samples at 1:1000. PLoS ONE (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; mouse; 1:100
EMD Millipore SLC12A5 antibody (Millipore, 07-432) was used in immunohistochemistry - free floating section on mouse samples at 1:100. J Comp Neurol (2012) ncbi
rabbit polyclonal
  • immunohistochemistry - free floating section; rat; 1:750
EMD Millipore SLC12A5 antibody (Upstate, 07-432) was used in immunohistochemistry - free floating section on rat samples at 1:750. J Comp Neurol (2008) ncbi
Articles Reviewed
  1. Wright R, Newey S, Ilie A, Wefelmeyer W, Raimondo J, Ginham R, et al. Neuronal Chloride Regulation via KCC2 Is Modulated through a GABAB Receptor Protein Complex. J Neurosci. 2017;37:5447-5462 pubmed publisher
  2. Forsberg D, Horn Z, Tserga E, Smedler E, Silberberg G, Shvarev Y, et al. CO2-evoked release of PGE2 modulates sighs and inspiration as demonstrated in brainstem organotypic culture. elife. 2016;5: pubmed publisher
  3. Wagner F, Bernard R, Derst C, French L, Veh R. Microarray analysis of transcripts with elevated expressions in the rat medial or lateral habenula suggest fast GABAergic excitation in the medial habenula and habenular involvement in the regulation of feeding and energy balance. Brain Struct Funct. 2016;221:4663-4689 pubmed
  4. Awad P, Sanon N, Chattopadhyaya B, Carriço J, Ouardouz M, Gagné J, et al. Reducing premature KCC2 expression rescues seizure susceptibility and spine morphology in atypical febrile seizures. Neurobiol Dis. 2016;91:10-20 pubmed publisher
  5. Javdani F, Holló K, Hegedűs K, Kis G, Hegyi Z, Dócs K, et al. Differential expression patterns of K(+) /Cl(-) cotransporter 2 in neurons within the superficial spinal dorsal horn of rats. J Comp Neurol. 2015;523:1967-83 pubmed publisher
  6. Toda T, Ishida K, Kiyama H, Yamashita T, Lee S. Down-regulation of KCC2 expression and phosphorylation in motoneurons, and increases the number of in primary afferent projections to motoneurons in mice with post-stroke spasticity. PLoS ONE. 2014;9:e114328 pubmed publisher
  7. Deidda G, Allegra M, Cerri C, Naskar S, Bony G, Zunino G, et al. Early depolarizing GABA controls critical-period plasticity in the rat visual cortex. Nat Neurosci. 2015;18:87-96 pubmed publisher
  8. Yassin L, Radtke Schuller S, Asraf H, Grothe B, Hershfinkel M, Forsythe I, et al. Nitric oxide signaling modulates synaptic inhibition in the superior paraolivary nucleus (SPN) via cGMP-dependent suppression of KCC2. Front Neural Circuits. 2014;8:65 pubmed publisher
  9. Ziemli ska E, K gler S, Schachner M, Wewi r I, Czarkowska Bauch J, Skup M. Overexpression of BDNF increases excitability of the lumbar spinal network and leads to robust early locomotor recovery in completely spinalized rats. PLoS ONE. 2014;9:e88833 pubmed publisher
  10. Wang X, Sun Q. Characterization of axo-axonic synapses in the piriform cortex of Mus musculus. J Comp Neurol. 2012;520:832-47 pubmed publisher
  11. Belenky M, Yarom Y, Pickard G. Heterogeneous expression of gamma-aminobutyric acid and gamma-aminobutyric acid-associated receptors and transporters in the rat suprachiasmatic nucleus. J Comp Neurol. 2008;506:708-32 pubmed