This is a Validated Antibody Database (VAD) review about dog RUVBL1, based on 4 published articles (read how Labome selects the articles), using RUVBL1 antibody in all methods. It is aimed to help Labome visitors find the most suited RUVBL1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RUVBL1 synonym: ruvB-like 1; RuvB-like 1

Knockout validation
Sigma-Aldrich
mouse monoclonal (5G3-11)
  • western blot knockout validation; mouse; 1:1000; fig 1
  • RNA immunoprecipitation; mouse; fig 7
  • chromatin immunoprecipitation; mouse; fig 2
  • immunoprecipitation; mouse; fig s3
Sigma-Aldrich RUVBL1 antibody (sigma, SAB4200194) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1), in RNA immunoprecipitation on mouse samples (fig 7), in chromatin immunoprecipitation on mouse samples (fig 2) and in immunoprecipitation on mouse samples (fig s3). Nat Commun (2015) ncbi
Sigma-Aldrich
mouse monoclonal (5G3-11)
  • western blot; human; loading ...; fig 4b
In order to examine the effects of the arginine methyltransferase PRMT5 on homologous recombination-mediated double-strand break repair, Sigma-Aldrich RUVBL1 antibody (Sigma, SAB4200194) was used in western blot on human samples (fig 4b). Mol Cell (2017) ncbi
mouse monoclonal (5G3-11)
  • immunoprecipitation; human; fig s4b
Sigma-Aldrich RUVBL1 antibody (Sigma-Aldrich, SAB4200194) was used in immunoprecipitation on human samples (fig s4b). Mol Cell Biol (2015) ncbi
mouse monoclonal (5G3-11)
  • western blot; mouse; 1:500; fig 6
Sigma-Aldrich RUVBL1 antibody (Sigma, SAB4200194) was used in western blot on mouse samples at 1:500 (fig 6). Nat Commun (2015) ncbi
mouse monoclonal (5G3-11)
  • western blot knockout validation; mouse; 1:1000; fig 1
  • RNA immunoprecipitation; mouse; fig 7
  • chromatin immunoprecipitation; mouse; fig 2
  • immunoprecipitation; mouse; fig s3
Sigma-Aldrich RUVBL1 antibody (sigma, SAB4200194) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1), in RNA immunoprecipitation on mouse samples (fig 7), in chromatin immunoprecipitation on mouse samples (fig 2) and in immunoprecipitation on mouse samples (fig s3). Nat Commun (2015) ncbi
Articles Reviewed
  1. Clarke T, Sanchez Bailon M, Chiang K, Reynolds J, Herrero Ruiz J, Bandeiras T, et al. PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination. Mol Cell. 2017;65:900-916.e7 pubmed publisher
  2. Mir R, Bele A, Mirza S, Srivastava S, Olou A, Ammons S, et al. A Novel Interaction of Ecdysoneless (ECD) Protein with R2TP Complex Component RUVBL1 Is Required for the Functional Role of ECD in Cell Cycle Progression. Mol Cell Biol. 2015;36:886-99 pubmed publisher
  3. Tarangelo A, Lo N, Teng R, Kim E, Le L, Watson D, et al. Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression. Nat Commun. 2015;6:10028 pubmed publisher
  4. Boo K, Bhin J, Jeon Y, Kim J, Shin H, Park J, et al. Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells. Nat Commun. 2015;6:6810 pubmed publisher